Aerobic C4-dicarboxylate transport protein - Escherichia coli (strain K12)

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P0A830 (DCTA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aerobic C4-dicarboxylate transport protein

Gene names

Name:	dctA
Ordered Locus Names:	b3528, JW3496

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the aerobic transport of the dicarboxylates fumarate, L- and D-malate and to a lesser extent succinate, from the periplasm across the inner membrane. Ref.6
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Induction	Subject to CRP-mediated catabolite repression. Repressed in the absence of oxygen, induced in the stationary phase and approximately 2-fold by citrate and C4-dicarboxylates. Ref.6
Disruption phenotype	Cells lacking this gene are only able to grow after a 2-day lag period on D-malate as sole carbon source. Ref.6
Sequence similarities	Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. [View classification]

Ontologies

Keywords
Biological process	Sugar transport Symport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-aspartate transport Inferred from direct assay PubMed 5999. Source: EcoCyc carbohydrate transport Inferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	integral to membrane Inferred from direct assay PubMed 357845. Source: EcoCyc plasma membrane Inferred from direct assay PubMed 1092298. Source: EcoCyc
Molecular_function	L-aspartate transmembrane transporter activity Inferred from direct assay PubMed 5999. Source: EcoCyc fumarate transmembrane transporter activity Inferred from mutant phenotype PubMed 4346810. Source: EcoCyc malate:hydrogen symporter activity Inferred from direct assay PubMed 5999. Source: EcoCyc sodium:dicarboxylate symporter activity Inferred from electronic annotation. Source: InterPro succinate transmembrane transporter activity Inferred from mutant phenotype PubMed 4346810. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 428

428

Aerobic C4-dicarboxylate transport protein HAMAP-Rule MF_01300

PRO_0000202094

Regions

Transmembrane

5 – 27

Helical; Potential

Transmembrane

47 – 64

Helical; Potential

Transmembrane

77 – 99

Helical; Potential

Transmembrane

141 – 163

Helical; Potential

Transmembrane

184 – 206

Helical; Potential

Transmembrane

219 – 241

Helical; Potential

Transmembrane

326 – 348

Helical; Potential

Transmembrane

352 – 374

Helical; Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P0A830 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: D9B32F987B62D234
Show »

FASTA

428

45,436

        10         20         30         40         50         60 
MKTSLFKSLY FQVLTAIAIG ILLGHFYPEI GEQMKPLGDG FVKLIKMIIA PVIFCTVVTG 

        70         80         90        100        110        120 
IAGMESMKAV GRTGAVALLY FEIVSTIALI IGLIIVNVVQ PGAGMNVDPA TLDAKAVAVY 

       130        140        150        160        170        180 
ADQAKDQGIV AFIMDVIPAS VIGAFASGNI LQVLLFAVLF GFALHRLGSK GQLIFNVIES 

       190        200        210        220        230        240 
FSQVIFGIIN MIMRLAPIGA FGAMAFTIGK YGVGTLVQLG QLIICFYITC ILFVVLVLGS 

       250        260        270        280        290        300 
IAKATGFSIF KFIRYIREEL LIVLGTSSSE SALPRMLDKM EKLGCRKSVV GLVIPTGYSF 

       310        320        330        340        350        360 
NLDGTSIYLT MAAVFIAQAT NSQMDIVHQI TLLIVLLLSS KGAAGVTGSG FIVLAATLSA 

       370        380        390        400        410        420 
VGHLPVAGLA LILGIDRFMS EARALTNLVG NGVATIVVAK WVKELDHKKL DDVLNNRAPD 


GKTHELSS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli." Davies S.J., Golby P., Omrani D., Broad S.A., Harrington V.L., Guest J.R., Kelly D.J., Andrews S.C. J. Bacteriol. 181:5624-5635(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Systems approach to refining genome annotation." Reed J.L., Patel T.R., Chen K.H., Joyce A.R., Applebee M.K., Herring C.D., Bui O.T., Knight E.M., Fong S.S., Palsson B.O. Proc. Natl. Acad. Sci. U.S.A. 103:17480-17484(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A D-MALATE TRANSPORTER, ROLE IN D-MALATE UTILIZATION, DISRUPTION PHENOTYPE, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18505.1. U00096 Genomic DNA. Translation: AAC76553.1. AP009048 Genomic DNA. Translation: BAE77766.1.
PIR	S47749.
RefSeq	NP_417985.1. NC_000913.2. YP_491907.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A830.
SMR	P0A830. Positions 19-400.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47958N.
IntAct	P0A830. 1 interaction.
MINT	MINT-1288518.
STRING	511145.b3528.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76553; AAC76553; b3528. BAE77766; BAE77766; BAE77766.
GeneID	12933165. 948039.
KEGG	ecj:Y75_p3649. eco:b3528.
PATRIC	32122520. VBIEscCol129921_3638.
Organism-specific databases
EchoBASE	EB4140.
EcoGene	EG20044. dctA.
Phylogenomic databases
eggNOG	COG1301.
HOGENOM	HOG000208775.
KO	K11103.
OMA	NIINMIM.
ProtClustDB	PRK01663.
Enzyme and pathway databases
BioCyc	EcoCyc:DCTA-MONOMER. ECOL316407:JW3496-MONOMER.
Gene expression databases
Genevestigator	P0A830.
Family and domain databases
HAMAP	MF_01300. C4_dicarb_transport.
InterPro	IPR023954. C4_dicarb_transport. IPR001991. Na-dicarboxylate_symporter. IPR018107. Na-dicarboxylate_symporter_CS. [Graphical view]
PANTHER	PTHR11958. PTHR11958. 1 hit.
Pfam	PF00375. SDF. 1 hit. [Graphical view]
PRINTS	PR00173. EDTRNSPORT.
PROSITE	PS00713. NA_DICARBOXYL_SYMP_1. 1 hit. PS00714. NA_DICARBOXYL_SYMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCTA_ECOLI

Accession

Primary (citable) accession number: P0A830
Secondary accession number(s): P37312, Q2M7J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents