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Protein

Aerobic C4-dicarboxylate transport protein

Gene

dctA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the aerobic transport of the dicarboxylates fumarate, L- and D-malate and to a lesser extent succinate, from the periplasm across the inner membrane.1 Publication

GO - Molecular functioni

  • fumarate transmembrane transporter activity Source: EcoCyc
  • L-aspartate transmembrane transporter activity Source: EcoCyc
  • malate:proton symporter activity Source: EcoCyc
  • succinate transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • fumarate transport Source: EcoliWiki
  • L-aspartate transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:DCTA-MONOMER.
ECOL316407:JW3496-MONOMER.
MetaCyc:DCTA-MONOMER.

Protein family/group databases

TCDBi2.A.23.1.7. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Aerobic C4-dicarboxylate transport protein
Gene namesi
Name:dctA
Ordered Locus Names:b3528, JW3496
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20044. dctA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2723HelicalSequence analysisAdd
BLAST
Transmembranei47 – 6418HelicalSequence analysisAdd
BLAST
Transmembranei77 – 9923HelicalSequence analysisAdd
BLAST
Transmembranei141 – 16323HelicalSequence analysisAdd
BLAST
Transmembranei184 – 20623HelicalSequence analysisAdd
BLAST
Transmembranei219 – 24123HelicalSequence analysisAdd
BLAST
Transmembranei326 – 34823HelicalSequence analysisAdd
BLAST
Transmembranei352 – 37423HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are only able to grow after a 2-day lag period on D-malate as sole carbon source.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Aerobic C4-dicarboxylate transport proteinPRO_0000202094Add
BLAST

Proteomic databases

EPDiP0A830.
PaxDbiP0A830.

Expressioni

Inductioni

Subject to CRP-mediated catabolite repression. Repressed in the absence of oxygen, induced in the stationary phase and approximately 2-fold by citrate and C4-dicarboxylates.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262159. 13 interactions.
DIPiDIP-47958N.
IntActiP0A830. 1 interaction.
MINTiMINT-1288518.
STRINGi511145.b3528.

Structurei

3D structure databases

ProteinModelPortaliP0A830.
SMRiP0A830. Positions 5-409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK2. Bacteria.
COG1301. LUCA.
HOGENOMiHOG000208775.
InParanoidiP0A830.
KOiK11103.
OMAiIKMERLG.
PhylomeDBiP0A830.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
HAMAPiMF_01300. C4_dicarb_transport. 1 hit.
InterProiIPR023954. C4_dicarb_transport.
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSLFKSLY FQVLTAIAIG ILLGHFYPEI GEQMKPLGDG FVKLIKMIIA
60 70 80 90 100
PVIFCTVVTG IAGMESMKAV GRTGAVALLY FEIVSTIALI IGLIIVNVVQ
110 120 130 140 150
PGAGMNVDPA TLDAKAVAVY ADQAKDQGIV AFIMDVIPAS VIGAFASGNI
160 170 180 190 200
LQVLLFAVLF GFALHRLGSK GQLIFNVIES FSQVIFGIIN MIMRLAPIGA
210 220 230 240 250
FGAMAFTIGK YGVGTLVQLG QLIICFYITC ILFVVLVLGS IAKATGFSIF
260 270 280 290 300
KFIRYIREEL LIVLGTSSSE SALPRMLDKM EKLGCRKSVV GLVIPTGYSF
310 320 330 340 350
NLDGTSIYLT MAAVFIAQAT NSQMDIVHQI TLLIVLLLSS KGAAGVTGSG
360 370 380 390 400
FIVLAATLSA VGHLPVAGLA LILGIDRFMS EARALTNLVG NGVATIVVAK
410 420
WVKELDHKKL DDVLNNRAPD GKTHELSS
Length:428
Mass (Da):45,436
Last modified:June 7, 2005 - v1
Checksum:iD9B32F987B62D234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18505.1.
U00096 Genomic DNA. Translation: AAC76553.1.
AP009048 Genomic DNA. Translation: BAE77766.1.
PIRiS47749.
RefSeqiNP_417985.1. NC_000913.3.
WP_000858214.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76553; AAC76553; b3528.
BAE77766; BAE77766; BAE77766.
GeneIDi948039.
KEGGiecj:JW3496.
eco:b3528.
PATRICi32122520. VBIEscCol129921_3638.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18505.1.
U00096 Genomic DNA. Translation: AAC76553.1.
AP009048 Genomic DNA. Translation: BAE77766.1.
PIRiS47749.
RefSeqiNP_417985.1. NC_000913.3.
WP_000858214.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A830.
SMRiP0A830. Positions 5-409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262159. 13 interactions.
DIPiDIP-47958N.
IntActiP0A830. 1 interaction.
MINTiMINT-1288518.
STRINGi511145.b3528.

Protein family/group databases

TCDBi2.A.23.1.7. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Proteomic databases

EPDiP0A830.
PaxDbiP0A830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76553; AAC76553; b3528.
BAE77766; BAE77766; BAE77766.
GeneIDi948039.
KEGGiecj:JW3496.
eco:b3528.
PATRICi32122520. VBIEscCol129921_3638.

Organism-specific databases

EchoBASEiEB4140.
EcoGeneiEG20044. dctA.

Phylogenomic databases

eggNOGiENOG4105CK2. Bacteria.
COG1301. LUCA.
HOGENOMiHOG000208775.
InParanoidiP0A830.
KOiK11103.
OMAiIKMERLG.
PhylomeDBiP0A830.

Enzyme and pathway databases

BioCyciEcoCyc:DCTA-MONOMER.
ECOL316407:JW3496-MONOMER.
MetaCyc:DCTA-MONOMER.

Miscellaneous databases

PROiP0A830.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
HAMAPiMF_01300. C4_dicarb_transport. 1 hit.
InterProiIPR023954. C4_dicarb_transport.
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCTA_ECOLI
AccessioniPrimary (citable) accession number: P0A830
Secondary accession number(s): P37312, Q2M7J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.