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Protein

Sugar fermentation stimulation protein A

Gene

sfsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds to DNA non-specifically. Could be a regulatory factor involved in maltose metabolism.UniRule annotation2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi201 – 22020H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoCyc

GO - Biological processi

  • positive regulation of transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10949-MONOMER.
ECOL316407:JW0142-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sugar fermentation stimulation protein AUniRule annotation
Gene namesi
Name:sfsAUniRule annotation
Synonyms:sfs1
Ordered Locus Names:b0146, JW0142
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10949. sfsA.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 234234Sugar fermentation stimulation protein APRO_0000152282Add
BLAST

Proteomic databases

PaxDbiP0A823.
PRIDEiP0A823.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
yehTP0AFT52EBI-556413,EBI-556431

Protein-protein interaction databases

BioGridi4259743. 19 interactions.
DIPiDIP-48084N.
IntActiP0A823. 7 interactions.
MINTiMINT-1271616.
STRINGi511145.b0146.

Structurei

Secondary structure

1
234
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 169Combined sources
Turni17 – 193Combined sources
Beta strandi20 – 245Combined sources
Beta strandi30 – 345Combined sources
Turni42 – 443Combined sources
Beta strandi50 – 556Combined sources
Beta strandi65 – 717Combined sources
Beta strandi77 – 793Combined sources
Helixi82 – 843Combined sources
Helixi85 – 9410Combined sources
Helixi99 – 1013Combined sources
Beta strandi105 – 1128Combined sources
Beta strandi119 – 1268Combined sources
Beta strandi129 – 13911Combined sources
Beta strandi141 – 1433Combined sources
Beta strandi146 – 1494Combined sources
Helixi155 – 16915Combined sources
Beta strandi173 – 1808Combined sources
Beta strandi187 – 1904Combined sources
Turni192 – 1943Combined sources
Helixi196 – 20712Combined sources
Beta strandi211 – 22010Combined sources
Beta strandi223 – 2297Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DAPX-ray2.20A1-234[»]
ProteinModelPortaliP0A823.
SMRiP0A823. Positions 1-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SfsA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105PW4. Bacteria.
COG1489. LUCA.
HOGENOMiHOG000218438.
InParanoidiP0A823.
KOiK06206.
OMAiMLYFINR.
OrthoDBiEOG6QZMTM.
PhylomeDBiP0A823.

Family and domain databases

HAMAPiMF_00095. SfsA.
InterProiIPR005224. SfsA.
[Graphical view]
PfamiPF03749. SfsA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00230. sfsA. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSPPLQRA TLIQRYKRFL ADVITPDGRE LTLHCPNTGA MTGCATPGDT
60 70 80 90 100
VWYSTSDNTK RKYPHTWELT QSQSGAFICV NTLWANRLTK EAILNESISE
110 120 130 140 150
LSGYSSLKSE VKYGAERSRI DFMLQADSRP DCYIEVKSVT LAENEQGYFP
160 170 180 190 200
DAVTERGQKH LRELMSVAAE GQRAVIFFAV LHSAITRFSP ARHIDEKYAQ
210 220 230
LLSEAQQRGV EILAYKAEIS AEGMALKKSL PVTL
Length:234
Mass (Da):26,229
Last modified:June 7, 2005 - v1
Checksum:i8AF528989FE78DCD
GO

Sequence cautioni

The sequence M34945 differs from that shown. Reason: Frameshift at position 160. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34945 Genomic DNA. No translation available.
M60726 Genomic DNA. Translation: AAA72977.1.
U00096 Genomic DNA. Translation: AAC73257.1.
AP009048 Genomic DNA. Translation: BAB96723.1.
PIRiA43671.
RefSeqiNP_414688.1. NC_000913.3.
WP_000396036.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73257; AAC73257; b0146.
BAB96723; BAB96723; BAB96723.
GeneIDi944855.
KEGGiecj:JW0142.
eco:b0146.
PATRICi32115399. VBIEscCol129921_0151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34945 Genomic DNA. No translation available.
M60726 Genomic DNA. Translation: AAA72977.1.
U00096 Genomic DNA. Translation: AAC73257.1.
AP009048 Genomic DNA. Translation: BAB96723.1.
PIRiA43671.
RefSeqiNP_414688.1. NC_000913.3.
WP_000396036.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DAPX-ray2.20A1-234[»]
ProteinModelPortaliP0A823.
SMRiP0A823. Positions 1-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259743. 19 interactions.
DIPiDIP-48084N.
IntActiP0A823. 7 interactions.
MINTiMINT-1271616.
STRINGi511145.b0146.

Proteomic databases

PaxDbiP0A823.
PRIDEiP0A823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73257; AAC73257; b0146.
BAB96723; BAB96723; BAB96723.
GeneIDi944855.
KEGGiecj:JW0142.
eco:b0146.
PATRICi32115399. VBIEscCol129921_0151.

Organism-specific databases

EchoBASEiEB0942.
EcoGeneiEG10949. sfsA.

Phylogenomic databases

eggNOGiENOG4105PW4. Bacteria.
COG1489. LUCA.
HOGENOMiHOG000218438.
InParanoidiP0A823.
KOiK06206.
OMAiMLYFINR.
OrthoDBiEOG6QZMTM.
PhylomeDBiP0A823.

Enzyme and pathway databases

BioCyciEcoCyc:EG10949-MONOMER.
ECOL316407:JW0142-MONOMER.

Miscellaneous databases

PROiP0A823.

Family and domain databases

HAMAPiMF_00095. SfsA.
InterProiIPR005224. SfsA.
[Graphical view]
PfamiPF03749. SfsA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00230. sfsA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation."
    Kang P.J., Craig E.A.
    J. Bacteriol. 172:2055-2064(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli."
    Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi Y.-L., Komano T.
    J. Bacteriol. 173:2644-2648(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Effects of the Escherichia coli sfsA gene on mal genes expression and a DNA binding activity of SfsA."
    Takeda K., Akimoto C., Kawamukai M.
    Biosci. Biotechnol. Biochem. 65:213-217(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSFSA_ECOLI
AccessioniPrimary (citable) accession number: P0A823
Secondary accession number(s): P18273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: January 20, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.