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Protein

L-seryl-tRNA(Sec) selenium transferase

Gene

selA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis. Requires selenophosphate as the selenium-donor molecule.2 Publications

Catalytic activityi

L-seryl-tRNA(Sec) + selenophosphate = L-selenocysteinyl-tRNA(Sec) + phosphate.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (bacterial route).
Proteins known to be involved in this subpathway in this organism are:
  1. L-seryl-tRNA(Sec) selenium transferase (selA)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (bacterial route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • L-seryl-tRNASec selenium transferase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

  • selenocysteine biosynthetic process Source: EcoCyc
  • selenocysteine incorporation Source: InterPro
  • selenocysteinyl-tRNA(Sec) biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processProtein biosynthesis
LigandPyridoxal phosphate, Selenium

Enzyme and pathway databases

BioCyciEcoCyc:EG10941-MONOMER
MetaCyc:EG10941-MONOMER
UniPathwayiUPA00906; UER00896

Names & Taxonomyi

Protein namesi
Recommended name:
L-seryl-tRNA(Sec) selenium transferase (EC:2.9.1.1)
Alternative name(s):
Selenocysteine synthase
Short name:
Sec synthase
Selenocysteinyl-tRNA(Sec) synthase
Gene namesi
Name:selA
Synonyms:fdhA
Ordered Locus Names:b3591, JW3564
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10941 selA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi224K → N: Still binds PLP. 1 Publication1
Mutagenesisi295K → N: Loss of activity and PLP binding. 1 Publication1
Mutagenesisi328K → N: Loss of PLP binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001895991 – 463L-seryl-tRNA(Sec) selenium transferaseAdd BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295N6-(pyridoxal phosphate)lysine1 Publication1

Proteomic databases

PaxDbiP0A821
PRIDEiP0A821

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers. Binds only one seryl-tRNA(Sec) per dimer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261876, 36 interactors
DIPiDIP-10847N
IntActiP0A821, 2 interactors
STRINGi316407.85676452

Structurei

3D structure databases

ProteinModelPortaliP0A821
SMRiP0A821
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SelA family.Curated

Phylogenomic databases

eggNOGiENOG4105C1Y Bacteria
COG1921 LUCA
HOGENOMiHOG000163726
InParanoidiP0A821
KOiK01042
OMAiLESFCVK
PhylomeDBiP0A821

Family and domain databases

Gene3Di3.40.640.10, 1 hit
HAMAPiMF_00423 SelA, 1 hit
InterProiView protein in InterPro
IPR015424 PyrdxlP-dep_Trfase
IPR015421 PyrdxlP-dep_Trfase_major
IPR018319 SelA-like
IPR004534 SelA_trans
IPR025862 SelA_trans_N_dom
PANTHERiPTHR32328:SF0 PTHR32328:SF0, 1 hit
PfamiView protein in Pfam
PF12390 Se-cys_synth_N, 1 hit
PF03841 SelA, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00474 selA, 1 hit

Sequencei

Sequence statusi: Complete.

P0A821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTETRSLYS QLPAIDRLLR DSSFLSLRDT YGHTRVVELL RQMLDEAREV
60 70 80 90 100
IRGSQTLPAW CENWAQEVDA RLTKEAQSAL RPVINLTGTV LHTNLGRALQ
110 120 130 140 150
AEAAVEAVAQ AMRSPVTLEY DLDDAGRGHR DRALAQLLCR ITGAEDACIV
160 170 180 190 200
NNNAAAVLLM LAATASGKEV VVSRGELVEI GGAFRIPDVM RQAGCTLHEV
210 220 230 240 250
GTTNRTHAND YRQAVNENTA LLMKVHTSNY SIQGFTKAID EAELVALGKE
260 270 280 290 300
LDVPVVTDLG SGSLVDLSQY GLPKEPMPQE LIAAGVSLVS FSGDKLLGGP
310 320 330 340 350
QAGIIVGKKE MIARLQSHPL KRALRADKMT LAALEATLRL YLHPEALSEK
360 370 380 390 400
LPTLRLLTRS AEVIQIQAQR LQAPLAAHYG AEFAVQVMPC LSQIGSGSLP
410 420 430 440 450
VDRLPSAALT FTPHDGRGSH LESLAARWRE LPVPVIGRIY DGRLWLDLRC
460
LEDEQRFLEM LLK
Length:463
Mass (Da):50,607
Last modified:June 7, 2005 - v1
Checksum:iCD724FF681D882DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7S → F in AAA24624 (PubMed:2007584).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64177 Genomic DNA Translation: AAA24624.1
U00039 Genomic DNA Translation: AAB18568.1
U00096 Genomic DNA Translation: AAC76615.1
AP009048 Genomic DNA Translation: BAE77702.1
PIRiA65159
RefSeqiNP_418048.1, NC_000913.3
WP_000206223.1, NZ_CP014272.1
WP_000206275.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76615; AAC76615; b3591
BAE77702; BAE77702; BAE77702
GeneIDi948124
KEGGiecj:JW3564
eco:b3591
PATRICifig|1411691.4.peg.3120

Similar proteinsi

Entry informationi

Entry nameiSELA_ECOLI
AccessioniPrimary (citable) accession number: P0A821
Secondary accession number(s): P23328
, P58225, P78119, Q2M7Q4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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