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P0A816

- RUVC_ECO57

UniProt

P0A816 - RUVC_ECO57

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Protein
Crossover junction endodeoxyribonuclease RuvC
Gene
ruvC, Z2915, ECs2573
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium By similarity
Metal bindingi67 – 671Magnesium By similarity
Metal bindingi139 – 1391Magnesium By similarity
Metal bindingi142 – 1421Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-2547-MONOMER.
ECOO157:RUVC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:Z2915, ECs2573
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 173172Crossover junction endodeoxyribonuclease RuvCUniRule annotation
PRO_0000183097Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z2915.

Structurei

3D structure databases

ProteinModelPortaliP0A816.
SMRiP0A816. Positions 2-159.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiRQVQEMV.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A816-1 [UniParc]FASTAAdd to Basket

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MAIILGIDPG SRVTGYGVIR QVGRQLSYLG SGCIRTKVDD LPSRLKLIYA    50
GVTEIITQFQ PDYFAIEQVF MAKNADSALK LGQARGVAIV AAVNQELPVF 100
EYAARQVKQT VVGIGSAEKS QVQHMVRTLL KLPANPQADA ADALAIAITH 150
CHVSQNAMQM SESRLNLARG RLR 173
Length:173
Mass (Da):18,747
Last modified:January 23, 2007 - v2
Checksum:i2170DCFA4AD43FE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56853.1.
BA000007 Genomic DNA. Translation: BAB35996.1.
PIRiA85799.
E90950.
RefSeqiNP_288300.1. NC_002655.2.
NP_310600.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG56853; AAG56853; Z2915.
BAB35996; BAB35996; BAB35996.
GeneIDi912572.
961836.
KEGGiece:Z2915.
ecs:ECs2573.
PATRICi18354518. VBIEscCol44059_2470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56853.1 .
BA000007 Genomic DNA. Translation: BAB35996.1 .
PIRi A85799.
E90950.
RefSeqi NP_288300.1. NC_002655.2.
NP_310600.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0A816.
SMRi P0A816. Positions 2-159.
ModBasei Search...

Protein-protein interaction databases

STRINGi 155864.Z2915.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG56853 ; AAG56853 ; Z2915 .
BAB35996 ; BAB35996 ; BAB35996 .
GeneIDi 912572.
961836.
KEGGi ece:Z2915.
ecs:ECs2573.
PATRICi 18354518. VBIEscCol44059_2470.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
KOi K01159.
OMAi RQVQEMV.
OrthoDBi EOG6RG044.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-2547-MONOMER.
ECOO157:RUVC-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiRUVC_ECO57
AccessioniPrimary (citable) accession number: P0A816
Secondary accession number(s): P24239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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