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P0A814 (RUVC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:b1863, JW1852
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length173 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group. Ref.6 Ref.7

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm. Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours. Ref.8

Sequence similarities

Belongs to the RuvC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6 Ref.7
Chain2 – 173172Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183096

Sites

Metal binding81Magnesium
Metal binding671Magnesium
Metal binding1391Magnesium
Metal binding1421Magnesium

Secondary structure

.......................... 173
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A814 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 2170DCFA4AD43FE1

FASTA17318,747
        10         20         30         40         50         60 
MAIILGIDPG SRVTGYGVIR QVGRQLSYLG SGCIRTKVDD LPSRLKLIYA GVTEIITQFQ 

        70         80         90        100        110        120 
PDYFAIEQVF MAKNADSALK LGQARGVAIV AAVNQELPVF EYAARQVKQT VVGIGSAEKS 

       130        140        150        160        170 
QVQHMVRTLL KLPANPQADA ADALAIAITH CHVSQNAMQM SESRLNLARG RLR 

« Hide

References

« Hide 'large scale' references
[1]"Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein."
Sharples G.J., Lloyd R.G.
J. Bacteriol. 173:7711-7715(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease."
Takahagi M., Iwasaki H., Nakata A., Shinagawa H.
J. Bacteriol. 173:5747-5753(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure."
Iwasaki H., Takahagi M., Shiba T., Nakata A., Shinagawa H.
EMBO J. 10:4381-4389(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-42, FUNCTION.
[7]"Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins."
Dunderdale H.J., Benson F.E., Parsons C.A., Sharples G.J., Lloyd R.G., West S.C.
Nature 354:506-510(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-25, FUNCTION.
[8]"A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair."
Babu M., Beloglazova N., Flick R., Graham C., Skarina T., Nocek B., Gagarinova A., Pogoutse O., Brown G., Binkowski A., Phanse S., Joachimiak A., Koonin E.V., Savchenko A., Emili A., Greenblatt J., Edwards A.M., Yakunin A.F.
Mol. Microbiol. 79:484-502(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12.
[9]"Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli."
Ariyoshi M., Vassylyev D.G., Iwasaki H., Nakamura H., Shinagawa H., Morikawa K.
Cell 78:1063-1072(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[10]"Processing of recombination intermediates by the RuvABC proteins."
West S.C.
Annu. Rev. Genet. 31:213-244(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59551 Genomic DNA. Translation: CAA42128.1.
D10165 Genomic DNA. Translation: BAA01032.1.
U00096 Genomic DNA. Translation: AAC74933.1.
AP009048 Genomic DNA. Translation: BAA15674.1.
PIRD38113.
RefSeqNP_416377.1. NC_000913.3.
YP_490125.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HJRX-ray2.50A/B/C/D2-159[»]
ProteinModelPortalP0A814.
SMRP0A814. Positions 2-159.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35952N.
IntActP0A814. 3 interactions.
STRING511145.b1863.

Proteomic databases

PaxDbP0A814.
PRIDEP0A814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74933; AAC74933; b1863.
BAA15674; BAA15674; BAA15674.
GeneID12934241.
946378.
KEGGecj:Y75_p1839.
eco:b1863.
PATRIC32119049. VBIEscCol129921_1942.

Organism-specific databases

EchoBASEEB0918.
EcoGeneEG10925. ruvC.

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMARQVQEMV.
OrthoDBEOG6RG044.
PhylomeDBP0A814.

Enzyme and pathway databases

BioCycEcoCyc:EG10925-MONOMER.
ECOL316407:JW1852-MONOMER.
MetaCyc:EG10925-MONOMER.

Gene expression databases

GenevestigatorP0A814.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0A814.
PROP0A814.

Entry information

Entry nameRUVC_ECOLI
AccessionPrimary (citable) accession number: P0A814
Secondary accession number(s): P24239
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene