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P0A814

- RUVC_ECOLI

UniProt

P0A814 - RUVC_ECOLI

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.2 Publications

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Magnesium
    Metal bindingi67 – 671Magnesium
    Metal bindingi139 – 1391Magnesium
    Metal bindingi142 – 1421Magnesium

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: EcoCyc
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro
    4. protein binding Source: EcoCyc

    GO - Biological processi

    1. DNA catabolic process, endonucleolytic Source: GOC
    2. recombinational repair Source: EcoCyc
    3. replication fork reversal Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10925-MONOMER.
    ECOL316407:JW1852-MONOMER.
    MetaCyc:EG10925-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease RuvC
    Holliday junction resolvase RuvC
    Gene namesi
    Name:ruvC
    Ordered Locus Names:b1863, JW1852
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10925. ruvC.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. Holliday junction resolvase complex Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 173172Crossover junction endodeoxyribonuclease RuvCPRO_0000183096Add
    BLAST

    Proteomic databases

    PaxDbiP0A814.
    PRIDEiP0A814.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A814.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-35952N.
    IntActiP0A814. 3 interactions.
    STRINGi511145.b1863.

    Structurei

    Secondary structure

    1
    173
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Beta strandi11 – 2212
    Beta strandi25 – 3511
    Helixi41 – 5919
    Beta strandi62 – 687
    Turni75 – 773
    Helixi78 – 9316
    Turni94 – 963
    Beta strandi99 – 1035
    Helixi104 – 1118
    Beta strandi113 – 1164
    Helixi119 – 12911
    Helixi141 – 15212
    Beta strandi155 – 1573

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HJRX-ray2.50A/B/C/D2-159[»]
    ProteinModelPortaliP0A814.
    SMRiP0A814. Positions 2-159.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A814.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.Curated

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012181.
    KOiK01159.
    OMAiRQVQEMV.
    OrthoDBiEOG6RG044.
    PhylomeDBiP0A814.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIILGIDPG SRVTGYGVIR QVGRQLSYLG SGCIRTKVDD LPSRLKLIYA    50
    GVTEIITQFQ PDYFAIEQVF MAKNADSALK LGQARGVAIV AAVNQELPVF 100
    EYAARQVKQT VVGIGSAEKS QVQHMVRTLL KLPANPQADA ADALAIAITH 150
    CHVSQNAMQM SESRLNLARG RLR 173
    Length:173
    Mass (Da):18,747
    Last modified:January 23, 2007 - v2
    Checksum:i2170DCFA4AD43FE1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59551 Genomic DNA. Translation: CAA42128.1.
    D10165 Genomic DNA. Translation: BAA01032.1.
    U00096 Genomic DNA. Translation: AAC74933.1.
    AP009048 Genomic DNA. Translation: BAA15674.1.
    PIRiD38113.
    RefSeqiNP_416377.1. NC_000913.3.
    YP_490125.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74933; AAC74933; b1863.
    BAA15674; BAA15674; BAA15674.
    GeneIDi12934241.
    946378.
    KEGGiecj:Y75_p1839.
    eco:b1863.
    PATRICi32119049. VBIEscCol129921_1942.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59551 Genomic DNA. Translation: CAA42128.1 .
    D10165 Genomic DNA. Translation: BAA01032.1 .
    U00096 Genomic DNA. Translation: AAC74933.1 .
    AP009048 Genomic DNA. Translation: BAA15674.1 .
    PIRi D38113.
    RefSeqi NP_416377.1. NC_000913.3.
    YP_490125.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HJR X-ray 2.50 A/B/C/D 2-159 [» ]
    ProteinModelPortali P0A814.
    SMRi P0A814. Positions 2-159.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35952N.
    IntActi P0A814. 3 interactions.
    STRINGi 511145.b1863.

    Proteomic databases

    PaxDbi P0A814.
    PRIDEi P0A814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74933 ; AAC74933 ; b1863 .
    BAA15674 ; BAA15674 ; BAA15674 .
    GeneIDi 12934241.
    946378.
    KEGGi ecj:Y75_p1839.
    eco:b1863.
    PATRICi 32119049. VBIEscCol129921_1942.

    Organism-specific databases

    EchoBASEi EB0918.
    EcoGenei EG10925. ruvC.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012181.
    KOi K01159.
    OMAi RQVQEMV.
    OrthoDBi EOG6RG044.
    PhylomeDBi P0A814.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10925-MONOMER.
    ECOL316407:JW1852-MONOMER.
    MetaCyc:EG10925-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A814.
    PROi P0A814.

    Gene expression databases

    Genevestigatori P0A814.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein."
      Sharples G.J., Lloyd R.G.
      J. Bacteriol. 173:7711-7715(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease."
      Takahagi M., Iwasaki H., Nakata A., Shinagawa H.
      J. Bacteriol. 173:5747-5753(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure."
      Iwasaki H., Takahagi M., Shiba T., Nakata A., Shinagawa H.
      EMBO J. 10:4381-4389(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-42, FUNCTION.
    7. "Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins."
      Dunderdale H.J., Benson F.E., Parsons C.A., Sharples G.J., Lloyd R.G., West S.C.
      Nature 354:506-510(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-25, FUNCTION.
    8. Cited for: SUBCELLULAR LOCATION.
      Strain: K12.
    9. "Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli."
      Ariyoshi M., Vassylyev D.G., Iwasaki H., Nakamura H., Shinagawa H., Morikawa K.
      Cell 78:1063-1072(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    10. "Processing of recombination intermediates by the RuvABC proteins."
      West S.C.
      Annu. Rev. Genet. 31:213-244(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRUVC_ECOLI
    AccessioniPrimary (citable) accession number: P0A814
    Secondary accession number(s): P24239
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3