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P0A814

- RUVC_ECOLI

UniProt

P0A814 - RUVC_ECOLI

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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.2 Publications

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Binds 1 magnesium ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium
Metal bindingi67 – 671Magnesium
Metal bindingi139 – 1391Magnesium
Metal bindingi142 – 1421Magnesium

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: EcoCyc
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA catabolic process, endonucleolytic Source: GOC
  2. recombinational repair Source: EcoCyc
  3. replication fork reversal Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10925-MONOMER.
ECOL316407:JW1852-MONOMER.
MetaCyc:EG10925-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:b1863, JW1852
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10925. ruvC.

Subcellular locationi

Cytoplasm 1 Publication
Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. Holliday junction resolvase complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 173172Crossover junction endodeoxyribonuclease RuvCPRO_0000183096Add
BLAST

Proteomic databases

PaxDbiP0A814.
PRIDEiP0A814.

Expressioni

Gene expression databases

GenevestigatoriP0A814.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-35952N.
IntActiP0A814. 3 interactions.
STRINGi511145.b1863.

Structurei

Secondary structure

1
173
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Beta strandi11 – 2212
Beta strandi25 – 3511
Helixi41 – 5919
Beta strandi62 – 687
Turni75 – 773
Helixi78 – 9316
Turni94 – 963
Beta strandi99 – 1035
Helixi104 – 1118
Beta strandi113 – 1164
Helixi119 – 12911
Helixi141 – 15212
Beta strandi155 – 1573

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HJRX-ray2.50A/B/C/D2-159[»]
ProteinModelPortaliP0A814.
SMRiP0A814. Positions 2-159.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A814.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.Curated

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
InParanoidiP0A814.
KOiK01159.
OMAiRQVQEMV.
OrthoDBiEOG6RG044.
PhylomeDBiP0A814.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A814-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIILGIDPG SRVTGYGVIR QVGRQLSYLG SGCIRTKVDD LPSRLKLIYA
60 70 80 90 100
GVTEIITQFQ PDYFAIEQVF MAKNADSALK LGQARGVAIV AAVNQELPVF
110 120 130 140 150
EYAARQVKQT VVGIGSAEKS QVQHMVRTLL KLPANPQADA ADALAIAITH
160 170
CHVSQNAMQM SESRLNLARG RLR
Length:173
Mass (Da):18,747
Last modified:January 23, 2007 - v2
Checksum:i2170DCFA4AD43FE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59551 Genomic DNA. Translation: CAA42128.1.
D10165 Genomic DNA. Translation: BAA01032.1.
U00096 Genomic DNA. Translation: AAC74933.1.
AP009048 Genomic DNA. Translation: BAA15674.1.
PIRiD38113.
RefSeqiNP_416377.1. NC_000913.3.
YP_490125.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74933; AAC74933; b1863.
BAA15674; BAA15674; BAA15674.
GeneIDi12934241.
946378.
KEGGiecj:Y75_p1839.
eco:b1863.
PATRICi32119049. VBIEscCol129921_1942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59551 Genomic DNA. Translation: CAA42128.1 .
D10165 Genomic DNA. Translation: BAA01032.1 .
U00096 Genomic DNA. Translation: AAC74933.1 .
AP009048 Genomic DNA. Translation: BAA15674.1 .
PIRi D38113.
RefSeqi NP_416377.1. NC_000913.3.
YP_490125.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HJR X-ray 2.50 A/B/C/D 2-159 [» ]
ProteinModelPortali P0A814.
SMRi P0A814. Positions 2-159.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35952N.
IntActi P0A814. 3 interactions.
STRINGi 511145.b1863.

Proteomic databases

PaxDbi P0A814.
PRIDEi P0A814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74933 ; AAC74933 ; b1863 .
BAA15674 ; BAA15674 ; BAA15674 .
GeneIDi 12934241.
946378.
KEGGi ecj:Y75_p1839.
eco:b1863.
PATRICi 32119049. VBIEscCol129921_1942.

Organism-specific databases

EchoBASEi EB0918.
EcoGenei EG10925. ruvC.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
InParanoidi P0A814.
KOi K01159.
OMAi RQVQEMV.
OrthoDBi EOG6RG044.
PhylomeDBi P0A814.

Enzyme and pathway databases

BioCyci EcoCyc:EG10925-MONOMER.
ECOL316407:JW1852-MONOMER.
MetaCyc:EG10925-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A814.
PROi P0A814.

Gene expression databases

Genevestigatori P0A814.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein."
    Sharples G.J., Lloyd R.G.
    J. Bacteriol. 173:7711-7715(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease."
    Takahagi M., Iwasaki H., Nakata A., Shinagawa H.
    J. Bacteriol. 173:5747-5753(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure."
    Iwasaki H., Takahagi M., Shiba T., Nakata A., Shinagawa H.
    EMBO J. 10:4381-4389(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-42, FUNCTION.
  7. "Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins."
    Dunderdale H.J., Benson F.E., Parsons C.A., Sharples G.J., Lloyd R.G., West S.C.
    Nature 354:506-510(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-25, FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION.
    Strain: K12.
  9. "Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli."
    Ariyoshi M., Vassylyev D.G., Iwasaki H., Nakamura H., Shinagawa H., Morikawa K.
    Cell 78:1063-1072(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  10. "Processing of recombination intermediates by the RuvABC proteins."
    West S.C.
    Annu. Rev. Genet. 31:213-244(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRUVC_ECOLI
AccessioniPrimary (citable) accession number: P0A814
Secondary accession number(s): P24239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3