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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.2 Publications

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Magnesium1
Metal bindingi67Magnesium1
Metal bindingi139Magnesium1
Metal bindingi142Magnesium1

GO - Molecular functioni

GO - Biological processi

  • recombinational repair Source: EcoCyc
  • response to radiation Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10925-MONOMER.
MetaCyc:EG10925-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:b1863, JW1852
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10925. ruvC.

Subcellular locationi

P0A814:

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Holliday junction resolvase complex Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001830962 – 173Crossover junction endodeoxyribonuclease RuvCAdd BLAST172

Proteomic databases

PaxDbiP0A814.
PRIDEiP0A814.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4263235. 95 interactors.
DIPiDIP-35952N.
IntActiP0A814. 3 interactors.
STRINGi316385.ECDH10B_2004.

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Beta strandi11 – 22Combined sources12
Beta strandi25 – 35Combined sources11
Helixi41 – 59Combined sources19
Beta strandi62 – 68Combined sources7
Turni75 – 77Combined sources3
Helixi78 – 93Combined sources16
Turni94 – 96Combined sources3
Beta strandi99 – 103Combined sources5
Helixi104 – 111Combined sources8
Beta strandi113 – 116Combined sources4
Helixi119 – 129Combined sources11
Helixi141 – 152Combined sources12
Beta strandi155 – 157Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HJRX-ray2.50A/B/C/D2-159[»]
ProteinModelPortaliP0A814.
SMRiP0A814.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A814.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.Curated

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012181.
InParanoidiP0A814.
KOiK01159.
PhylomeDBiP0A814.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiView protein in InterPro
IPR012337. RNaseH-like_sf.
IPR036397. RNaseH_sf.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
PANTHERiPTHR30194. PTHR30194. 1 hit.
PfamiView protein in Pfam
PF02075. RuvC. 1 hit.
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiView protein in PROSITE
PS01321. RUVC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIILGIDPG SRVTGYGVIR QVGRQLSYLG SGCIRTKVDD LPSRLKLIYA
60 70 80 90 100
GVTEIITQFQ PDYFAIEQVF MAKNADSALK LGQARGVAIV AAVNQELPVF
110 120 130 140 150
EYAARQVKQT VVGIGSAEKS QVQHMVRTLL KLPANPQADA ADALAIAITH
160 170
CHVSQNAMQM SESRLNLARG RLR
Length:173
Mass (Da):18,747
Last modified:January 23, 2007 - v2
Checksum:i2170DCFA4AD43FE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59551 Genomic DNA. Translation: CAA42128.1.
D10165 Genomic DNA. Translation: BAA01032.1.
U00096 Genomic DNA. Translation: AAC74933.1.
AP009048 Genomic DNA. Translation: BAA15674.1.
PIRiD38113.
RefSeqiNP_416377.1. NC_000913.3.
WP_001295503.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74933; AAC74933; b1863.
BAA15674; BAA15674; BAA15674.
GeneIDi946378.
KEGGiecj:JW1852.
eco:b1863.
PATRICifig|1411691.4.peg.385.

Similar proteinsi

Entry informationi

Entry nameiRUVC_ECOLI
AccessioniPrimary (citable) accession number: P0A814
Secondary accession number(s): P24239
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families