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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation1 Publication

Enzyme regulationi

RuvB possesses weak ATPase activity, which is stimulated by the RuvA protein in the presence of DNA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 69ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: CACAO
  • DNA binding Source: InterPro
  • four-way junction helicase activity Source: EcoCyc

GO - Biological processi

  • recombinational repair Source: EcoCyc
  • SOS response Source: EcoCyc

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10924-MONOMER.
MetaCyc:EG10924-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:b1860, JW1849
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10924. ruvB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • Holliday junction resolvase complex Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001655281 – 336Holliday junction ATP-dependent DNA helicase RuvBAdd BLAST336

Proteomic databases

PaxDbiP0A812.
PRIDEiP0A812.

Expressioni

Inductioni

Expression of the ruv region is induced by damage to DNA and is regulated by LexA as part of the SOS response. RuvA and RuvB are also involved in mutagenesis induced by UV and X irradiation and by some chemicals (PubMed:3279394). Induced by hydroxyurea (PubMed:20005847).2 Publications

Interactioni

Subunit structurei

Homododecamer composed of two hexameric rings; when bound to DNA in the presence of ATP and magnesium. Forms a complex with RuvA. Interacts with YgbT (Cas1).1 Publication

Protein-protein interaction databases

BioGridi4260882. 80 interactors.
DIPiDIP-47870N.
IntActiP0A812. 15 interactors.
MINTiMINT-1266054.
STRINGi316385.ECDH10B_2001.

Structurei

3D structure databases

ProteinModelPortaliP0A812.
SMRiP0A812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
HOGENOMiHOG000218623.
InParanoidiP0A812.
KOiK03551.
PhylomeDBiP0A812.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR42848. PTHR42848. 1 hit.
PfamiView protein in Pfam
PF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL
60 70 80 90 100
RGDALDHLLI FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM
110 120 130 140 150
LTNLEPHDVL FIDEIHRLSP VVEEVLYPAM EDYQLDIMIG EGPAARSIKI
160 170 180 190 200
DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR LEFYQVPDLQ YIVSRSARFM
210 220 230 240 250
GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG TISADIAAQA
260 270 280 290 300
LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV
310 320 330
LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP
Length:336
Mass (Da):37,174
Last modified:August 1, 1988 - v1
Checksum:i79BAB4A9A5687F7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07091 Genomic DNA. Translation: CAA30120.1.
M21298 Genomic DNA. Translation: AAA24613.1.
U00096 Genomic DNA. Translation: AAC74930.1.
AP009048 Genomic DNA. Translation: BAA15671.1.
PIRiB28533. BVECVB.
RefSeqiNP_416374.1. NC_000913.3.
WP_000568519.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74930; AAC74930; b1860.
BAA15671; BAA15671; BAA15671.
GeneIDi946371.
KEGGiecj:JW1849.
eco:b1860.
PATRICifig|1411691.4.peg.388.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRUVB_ECOLI
AccessioniPrimary (citable) accession number: P0A812
Secondary accession number(s): P08577
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 5, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families