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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation1 Publication

Enzyme regulationi

RuvB possesses weak ATPase activity, which is stimulated by the RuvA protein in the presence of DNA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 698ATPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • ATP-dependent DNA helicase activity Source: CACAO
  • DNA binding Source: InterPro
  • four-way junction helicase activity Source: EcoCyc

GO - Biological processi

  • DNA duplex unwinding Source: GOC
  • recombinational repair Source: EcoCyc
  • SOS response Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10924-MONOMER.
ECOL316407:JW1849-MONOMER.
MetaCyc:EG10924-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:b1860, JW1849
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10924. ruvB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Holliday junction resolvase complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Holliday junction ATP-dependent DNA helicase RuvBPRO_0000165528Add
BLAST

Proteomic databases

PaxDbiP0A812.

Expressioni

Inductioni

Expression of the ruv region is induced by damage to DNA and is regulated by LexA as part of the SOS response. RuvA and RuvB are also involved in mutagenesis induced by UV and X irradiation and by some chemicals (PubMed:3279394). Induced by hydroxyurea (PubMed:20005847).2 Publications

Interactioni

Subunit structurei

Homododecamer composed of two hexameric rings; when bound to DNA in the presence of ATP and magnesium. Forms a complex with RuvA. Interacts with YgbT (Cas1).1 Publication

Protein-protein interaction databases

DIPiDIP-47870N.
IntActiP0A812. 15 interactions.
MINTiMINT-1266054.
STRINGi511145.b1860.

Structurei

3D structure databases

ProteinModelPortaliP0A812.
SMRiP0A812. Positions 22-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2255.
HOGENOMiHOG000218623.
InParanoidiP0A812.
KOiK03551.
OMAiDRFGMSF.
OrthoDBiEOG6SR93S.
PhylomeDBiP0A812.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL
60 70 80 90 100
RGDALDHLLI FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM
110 120 130 140 150
LTNLEPHDVL FIDEIHRLSP VVEEVLYPAM EDYQLDIMIG EGPAARSIKI
160 170 180 190 200
DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR LEFYQVPDLQ YIVSRSARFM
210 220 230 240 250
GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG TISADIAAQA
260 270 280 290 300
LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV
310 320 330
LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP
Length:336
Mass (Da):37,174
Last modified:August 1, 1988 - v1
Checksum:i79BAB4A9A5687F7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07091 Genomic DNA. Translation: CAA30120.1.
M21298 Genomic DNA. Translation: AAA24613.1.
U00096 Genomic DNA. Translation: AAC74930.1.
AP009048 Genomic DNA. Translation: BAA15671.1.
PIRiB28533. BVECVB.
RefSeqiNP_416374.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74930; AAC74930; b1860.
BAA15671; BAA15671; BAA15671.
GeneIDi946371.
KEGGiecj:Y75_p1836.
eco:b1860.
PATRICi32119043. VBIEscCol129921_1939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07091 Genomic DNA. Translation: CAA30120.1.
M21298 Genomic DNA. Translation: AAA24613.1.
U00096 Genomic DNA. Translation: AAC74930.1.
AP009048 Genomic DNA. Translation: BAA15671.1.
PIRiB28533. BVECVB.
RefSeqiNP_416374.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0A812.
SMRiP0A812. Positions 22-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47870N.
IntActiP0A812. 15 interactions.
MINTiMINT-1266054.
STRINGi511145.b1860.

Proteomic databases

PaxDbiP0A812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74930; AAC74930; b1860.
BAA15671; BAA15671; BAA15671.
GeneIDi946371.
KEGGiecj:Y75_p1836.
eco:b1860.
PATRICi32119043. VBIEscCol129921_1939.

Organism-specific databases

EchoBASEiEB0917.
EcoGeneiEG10924. ruvB.

Phylogenomic databases

eggNOGiCOG2255.
HOGENOMiHOG000218623.
InParanoidiP0A812.
KOiK03551.
OMAiDRFGMSF.
OrthoDBiEOG6SR93S.
PhylomeDBiP0A812.

Enzyme and pathway databases

BioCyciEcoCyc:EG10924-MONOMER.
ECOL316407:JW1849-MONOMER.
MetaCyc:EG10924-MONOMER.

Miscellaneous databases

PROiP0A812.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes."
    Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G.
    Nucleic Acids Res. 16:1541-1549(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: K12.
  2. "Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination."
    Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.
    J. Bacteriol. 170:4322-4329(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro."
    Tsaneva I.R., Mueller B., West S.C.
    Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
  7. "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli."
    Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J., Walker G.C.
    Mol. Cell 36:845-860(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYDROXYUREA.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  8. Cited for: INTERACTION WITH YGBT, SUBCELLULAR LOCATION.
    Strain: K12.
  9. "Processing of recombination intermediates by the RuvABC proteins."
    West S.C.
    Annu. Rev. Genet. 31:213-244(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRUVB_ECOLI
AccessioniPrimary (citable) accession number: P0A812
Secondary accession number(s): P08577
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.