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P0A812

- RUVB_ECOLI

UniProt

P0A812 - RUVB_ECOLI

Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.1 PublicationUniRule annotation

    Catalytic activityi

    ATP + H2O = ADP + phosphate.1 PublicationUniRule annotation

    Enzyme regulationi

    RuvB possesses weak ATPase activity, which is stimulated by the RuvA protein in the presence of DNA.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 698ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. ATP-dependent DNA helicase activity Source: CACAO
    3. DNA binding Source: InterPro
    4. four-way junction helicase activity Source: EcoCyc
    5. protein binding Source: EcoCyc

    GO - Biological processi

    1. DNA duplex unwinding Source: GOC
    2. recombinational repair Source: EcoCyc
    3. SOS response Source: EcoCyc

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, SOS response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10924-MONOMER.
    ECOL316407:JW1849-MONOMER.
    MetaCyc:EG10924-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
    Gene namesi
    Name:ruvBUniRule annotation
    Ordered Locus Names:b1860, JW1849
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10924. ruvB.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. Holliday junction resolvase complex Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Holliday junction ATP-dependent DNA helicase RuvBPRO_0000165528Add
    BLAST

    Proteomic databases

    PaxDbiP0A812.

    Expressioni

    Inductioni

    Expression of the ruv region is induced by damage to DNA and is regulated by LexA as part of the SOS response. RuvA and RuvB are also involved in mutagenesis induced by UV and X irradiation and by some chemicals.1 Publication

    Gene expression databases

    GenevestigatoriP0A812.

    Interactioni

    Subunit structurei

    Homododecamer composed of two hexameric rings; when bound to DNA in the presence of ATP and magnesium. Forms a complex with RuvA. Interacts with YgbT (Cas1).1 Publication

    Protein-protein interaction databases

    DIPiDIP-47870N.
    IntActiP0A812. 15 interactions.
    MINTiMINT-1266054.
    STRINGi511145.b1860.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A812.
    SMRiP0A812. Positions 22-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2255.
    HOGENOMiHOG000218623.
    KOiK03551.
    OMAiDYAEVKH.
    OrthoDBiEOG6SR93S.
    PhylomeDBiP0A812.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00016. DNA_helic_RuvB.
    InterProiIPR003593. AAA+_ATPase.
    IPR004605. DNA_helicase_Holl-junc_RuvB.
    IPR008823. DNA_helicase_Holl-junc_RuvB_C.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF05491. RuvB_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view]
    ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00635. ruvB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A812-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL    50
    RGDALDHLLI FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM 100
    LTNLEPHDVL FIDEIHRLSP VVEEVLYPAM EDYQLDIMIG EGPAARSIKI 150
    DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR LEFYQVPDLQ YIVSRSARFM 200
    GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG TISADIAAQA 250
    LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV 300
    LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP 336
    Length:336
    Mass (Da):37,174
    Last modified:August 1, 1988 - v1
    Checksum:i79BAB4A9A5687F7C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07091 Genomic DNA. Translation: CAA30120.1.
    M21298 Genomic DNA. Translation: AAA24613.1.
    U00096 Genomic DNA. Translation: AAC74930.1.
    AP009048 Genomic DNA. Translation: BAA15671.1.
    PIRiB28533. BVECVB.
    RefSeqiNP_416374.1. NC_000913.3.
    YP_490122.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74930; AAC74930; b1860.
    BAA15671; BAA15671; BAA15671.
    GeneIDi12931888.
    946371.
    KEGGiecj:Y75_p1836.
    eco:b1860.
    PATRICi32119043. VBIEscCol129921_1939.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07091 Genomic DNA. Translation: CAA30120.1 .
    M21298 Genomic DNA. Translation: AAA24613.1 .
    U00096 Genomic DNA. Translation: AAC74930.1 .
    AP009048 Genomic DNA. Translation: BAA15671.1 .
    PIRi B28533. BVECVB.
    RefSeqi NP_416374.1. NC_000913.3.
    YP_490122.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A812.
    SMRi P0A812. Positions 22-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47870N.
    IntActi P0A812. 15 interactions.
    MINTi MINT-1266054.
    STRINGi 511145.b1860.

    Proteomic databases

    PaxDbi P0A812.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74930 ; AAC74930 ; b1860 .
    BAA15671 ; BAA15671 ; BAA15671 .
    GeneIDi 12931888.
    946371.
    KEGGi ecj:Y75_p1836.
    eco:b1860.
    PATRICi 32119043. VBIEscCol129921_1939.

    Organism-specific databases

    EchoBASEi EB0917.
    EcoGenei EG10924. ruvB.

    Phylogenomic databases

    eggNOGi COG2255.
    HOGENOMi HOG000218623.
    KOi K03551.
    OMAi DYAEVKH.
    OrthoDBi EOG6SR93S.
    PhylomeDBi P0A812.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10924-MONOMER.
    ECOL316407:JW1849-MONOMER.
    MetaCyc:EG10924-MONOMER.

    Miscellaneous databases

    PROi P0A812.

    Gene expression databases

    Genevestigatori P0A812.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00016. DNA_helic_RuvB.
    InterProi IPR003593. AAA+_ATPase.
    IPR004605. DNA_helicase_Holl-junc_RuvB.
    IPR008823. DNA_helicase_Holl-junc_RuvB_C.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF05491. RuvB_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view ]
    ProDomi PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00635. ruvB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes."
      Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G.
      Nucleic Acids Res. 16:1541-1549(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: K12.
    2. "Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination."
      Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.
      J. Bacteriol. 170:4322-4329(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro."
      Tsaneva I.R., Mueller B., West S.C.
      Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
    7. Cited for: INTERACTION WITH YGBT, SUBCELLULAR LOCATION.
      Strain: K12.
    8. "Processing of recombination intermediates by the RuvABC proteins."
      West S.C.
      Annu. Rev. Genet. 31:213-244(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRUVB_ECOLI
    AccessioniPrimary (citable) accession number: P0A812
    Secondary accession number(s): P08577
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3