P0A812 (RUVB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:	ruvB
Ordered Locus Names:	b1860, JW1849

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. Ref.6
Catalytic activity	ATP + H2O = ADP + phosphate. Ref.6
Enzyme regulation	RuvB possesses weak ATPase activity, which is stimulated by the RuvA protein in the presence of DNA. Ref.6
Subunit structure	Homododecamer composed of two hexameric rings; when bound to DNA in the presence of ATP and magnesium. Forms a complex with RuvA. Interacts with YgbT (Cas1). Ref.7
Subcellular location	Cytoplasm. Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours. Ref.7
Induction	Expression of the ruv region is induced by damage to DNA and is regulated by LexA as part of the SOS response. RuvA and RuvB are also involved in mutagenesis induced by UV and X irradiation and by some chemicals. Ref.1 Ref.6
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SOS response Inferred from expression pattern Ref.2. Source: EcoCyc recombinational repair Inferred from mutant phenotype PubMed 6374379. Source: EcoCyc
Cellular_component	Holliday junction resolvase complex Inferred from direct assay PubMed 9160752. Source: EcoCyc cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from direct assay PubMed 1608954PubMed 1617728. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Holliday junction ATP-dependent DNA helicase RuvB HAMAP-Rule MF_00016		PRO_0000165528
Regions
	Nucleotide binding	62 – 69	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A812 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 79BAB4A9A5687F7C	FASTA	336	37,174
	10 20 30 40 50 60 MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL RGDALDHLLI 70 80 90 100 110 120 FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM LTNLEPHDVL FIDEIHRLSP 130 140 150 160 170 180 VVEEVLYPAM EDYQLDIMIG EGPAARSIKI DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR 190 200 210 220 230 240 LEFYQVPDLQ YIVSRSARFM GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG 250 260 270 280 290 300 TISADIAAQA LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV 310 320 330 LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes." Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G. Nucleic Acids Res. 16:1541-1549(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: K12.
[2]	"Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination." Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A. J. Bacteriol. 170:4322-4329(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro." Tsaneva I.R., Mueller B., West S.C. Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
[7]	"A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair." Babu M., Beloglazova N., Flick R., Graham C., Skarina T., Nocek B., Gagarinova A., Pogoutse O., Brown G., Binkowski A., Phanse S., Joachimiak A., Koonin E.V., Savchenko A., Emili A., Greenblatt J., Edwards A.M., Yakunin A.F. Mol. Microbiol. 79:484-502(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YGBT, SUBCELLULAR LOCATION. Strain: K12.
[8]	"Processing of recombination intermediates by the RuvABC proteins." West S.C. Annu. Rev. Genet. 31:213-244(1997) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07091 Genomic DNA. Translation: CAA30120.1. M21298 Genomic DNA. Translation: AAA24613.1. U00096 Genomic DNA. Translation: AAC74930.1. AP009048 Genomic DNA. Translation: BAA15671.1.
PIR	BVECVB. B28533.
RefSeq	NP_416374.1. NC_000913.2. YP_490122.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A812.
SMR	P0A812. Positions 22-333.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47870N.
IntAct	P0A812. 15 interactions.
MINT	MINT-1266054.
STRING	511145.b1860.
Proteomic databases
PaxDb	P0A812.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74930; AAC74930; b1860. BAA15671; BAA15671; BAA15671.
GeneID	12931888. 946371.
KEGG	ecj:Y75_p1836. eco:b1860.
PATRIC	32119043. VBIEscCol129921_1939.
Organism-specific databases
EchoBASE	EB0917.
EcoGene	EG10924. ruvB.
Phylogenomic databases
eggNOG	COG2255.
HOGENOM	HOG000218623.
KO	K03551.
OMA	HIIAKEM.
ProtClustDB	PRK00080.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10924-MONOMER. ECOL316407:JW1849-MONOMER. MetaCyc:EG10924-MONOMER.
Gene expression databases
Genevestigator	P0A812.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00016. DNA_helic_RuvB.
InterPro	IPR003593. AAA+_ATPase. IPR004605. DNA_helicase_Holl-junc_RuvB. IPR008823. DNA_helicase_Holl-junc_RuvB_C. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13779:SF2. PTHR13779:SF2. 1 hit.
Pfam	PF05491. RuvB_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
ProDom	PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00635. ruvB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVB_ECOLI

Accession

Primary (citable) accession number: P0A812
Secondary accession number(s): P08577

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program