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Protein

Holliday junction ATP-dependent DNA helicase RuvA

Gene

ruvA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • four-way junction helicase activity Source: EcoCyc

GO - Biological processi

  • recombinational repair Source: EcoCyc
  • SOS response Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10923-MONOMER.
ECOL316407:JW1850-MONOMER.
MetaCyc:EG10923-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvAUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvAUniRule annotation
Ordered Locus Names:b1861, JW1850
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10923. ruvA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Holliday junction helicase complex Source: EcoCyc
  • Holliday junction resolvase complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000946291 – 203Holliday junction ATP-dependent DNA helicase RuvAAdd BLAST203

Proteomic databases

PaxDbiP0A809.
PRIDEiP0A809.

Expressioni

Inductioni

Expression of the ruv region is induced by damage to DNA and is regulated by LexA as part of the SOS response. RuvA and RuvB are also involved in mutagenesis induced by UV and X irradiation and by some chemicals (PubMed:3279394). Induced by hydroxyurea (PubMed:20005847).2 Publications

Interactioni

Subunit structurei

Homotetramer; forms a complex with RuvB.2 Publications

Protein-protein interaction databases

BioGridi4259338. 130 interactors.
DIPiDIP-48064N.
IntActiP0A809. 7 interactors.
MINTiMINT-1321234.
STRINGi511145.b1861.

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 13Combined sources10
Beta strandi16 – 21Combined sources6
Beta strandi24 – 29Combined sources6
Helixi32 – 35Combined sources4
Beta strandi43 – 54Combined sources12
Beta strandi57 – 65Combined sources9
Helixi66 – 77Combined sources12
Beta strandi78 – 80Combined sources3
Helixi83 – 92Combined sources10
Helixi95 – 103Combined sources9
Helixi107 – 111Combined sources5
Helixi118 – 131Combined sources14
Helixi132 – 134Combined sources3
Turni138 – 140Combined sources3
Helixi144 – 149Combined sources6
Helixi158 – 170Combined sources13
Helixi174 – 183Combined sources10
Helixi191 – 200Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BDXX-ray6.00A/B/C/D1-203[»]
1C7YX-ray3.10A1-203[»]
1CUKX-ray1.90A1-203[»]
1D8LX-ray2.50A/B1-149[»]
1HJPX-ray2.50A1-203[»]
ProteinModelPortaliP0A809.
SMRiP0A809.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A809.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KA4. Bacteria.
COG0632. LUCA.
HOGENOMiHOG000057116.
InParanoidiP0A809.
KOiK03550.
OMAiVGYQVHC.
PhylomeDBiP0A809.

Family and domain databases

CDDicd14332. UBA_RuvA_C. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA. 1 hit.
InterProiIPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
IPR011114. RuvA_C.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A809-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGRLRGIII EKQPPLVLIE VGGVGYEVHM PMTCFYELPE AGQEAIVFTH
60 70 80 90 100
FVVREDAQLL YGFNNKQERT LFKELIKTNG VGPKLALAIL SGMSAQQFVN
110 120 130 140 150
AVEREEVGAL VKLPGIGKKT AERLIVEMKD RFKGLHGDLF TPAADLVLTS
160 170 180 190 200
PASPATDDAE QEAVAALVAL GYKPQEASRM VSKIARPDAS SETLIREALR

AAL
Length:203
Mass (Da):22,086
Last modified:June 7, 2005 - v1
Checksum:i805DDB79DC5A8385
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166A → R in CAA30119 (PubMed:3279394).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07091 Genomic DNA. Translation: CAA30119.1.
M21298 Genomic DNA. Translation: AAA24612.1.
U00096 Genomic DNA. Translation: AAC74931.1.
AP009048 Genomic DNA. Translation: BAA15672.1.
D10165 Genomic DNA. Translation: BAA01034.1.
PIRiE64948. BVECRV.
RefSeqiNP_416375.1. NC_000913.3.
WP_000580323.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74931; AAC74931; b1861.
BAA15672; BAA15672; BAA15672.
GeneIDi946369.
KEGGiecj:JW1850.
eco:b1861.
PATRICi32119045. VBIEscCol129921_1940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07091 Genomic DNA. Translation: CAA30119.1.
M21298 Genomic DNA. Translation: AAA24612.1.
U00096 Genomic DNA. Translation: AAC74931.1.
AP009048 Genomic DNA. Translation: BAA15672.1.
D10165 Genomic DNA. Translation: BAA01034.1.
PIRiE64948. BVECRV.
RefSeqiNP_416375.1. NC_000913.3.
WP_000580323.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BDXX-ray6.00A/B/C/D1-203[»]
1C7YX-ray3.10A1-203[»]
1CUKX-ray1.90A1-203[»]
1D8LX-ray2.50A/B1-149[»]
1HJPX-ray2.50A1-203[»]
ProteinModelPortaliP0A809.
SMRiP0A809.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259338. 130 interactors.
DIPiDIP-48064N.
IntActiP0A809. 7 interactors.
MINTiMINT-1321234.
STRINGi511145.b1861.

Proteomic databases

PaxDbiP0A809.
PRIDEiP0A809.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74931; AAC74931; b1861.
BAA15672; BAA15672; BAA15672.
GeneIDi946369.
KEGGiecj:JW1850.
eco:b1861.
PATRICi32119045. VBIEscCol129921_1940.

Organism-specific databases

EchoBASEiEB0916.
EcoGeneiEG10923. ruvA.

Phylogenomic databases

eggNOGiENOG4105KA4. Bacteria.
COG0632. LUCA.
HOGENOMiHOG000057116.
InParanoidiP0A809.
KOiK03550.
OMAiVGYQVHC.
PhylomeDBiP0A809.

Enzyme and pathway databases

BioCyciEcoCyc:EG10923-MONOMER.
ECOL316407:JW1850-MONOMER.
MetaCyc:EG10923-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A809.
PROiP0A809.

Family and domain databases

CDDicd14332. UBA_RuvA_C. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA. 1 hit.
InterProiIPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
IPR011114. RuvA_C.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVA_ECOLI
AccessioniPrimary (citable) accession number: P0A809
Secondary accession number(s): P08576
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.