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P0A809

- RUVA_ECOLI

UniProt

P0A809 - RUVA_ECOLI

Protein

Holliday junction ATP-dependent DNA helicase RuvA

Gene

ruvA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.1 PublicationUniRule annotation

    Catalytic activityi

    ATP + H2O = ADP + phosphate.1 PublicationUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. four-way junction helicase activity Source: EcoCyc

    GO - Biological processi

    1. DNA duplex unwinding Source: GOC
    2. recombinational repair Source: EcoCyc
    3. SOS response Source: EcoCyc

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, SOS response

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10923-MONOMER.
    ECOL316407:JW1850-MONOMER.
    MetaCyc:EG10923-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Holliday junction ATP-dependent DNA helicase RuvAUniRule annotation (EC:3.6.4.12UniRule annotation)
    Gene namesi
    Name:ruvAUniRule annotation
    Ordered Locus Names:b1861, JW1850
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10923. ruvA.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: In 15% of cell localizes to discrete nucleoid foci (probable DNA damage sites) upon treatment with mitomycin C (MMC) for 2 hours.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. Holliday junction helicase complex Source: EcoCyc
    3. Holliday junction resolvase complex Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 203203Holliday junction ATP-dependent DNA helicase RuvAPRO_0000094629Add
    BLAST

    Proteomic databases

    PaxDbiP0A809.
    PRIDEiP0A809.

    Expressioni

    Inductioni

    Expression of the ruv region is induced by damage to DNA and is regulated by LexA as part of the SOS response. RuvA and RuvB are also involved in mutagenesis induced by UV and X irradiation and by some chemicals.1 Publication

    Gene expression databases

    GenevestigatoriP0A809.

    Interactioni

    Subunit structurei

    Homotetramer; forms a complex with RuvB.2 Publications

    Protein-protein interaction databases

    DIPiDIP-48064N.
    IntActiP0A809. 7 interactions.
    MINTiMINT-1321234.
    STRINGi511145.b1861.

    Structurei

    Secondary structure

    1
    203
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 1310
    Beta strandi16 – 216
    Beta strandi24 – 296
    Helixi32 – 354
    Beta strandi43 – 5412
    Beta strandi57 – 659
    Helixi66 – 7712
    Beta strandi78 – 803
    Helixi83 – 9210
    Helixi95 – 1039
    Helixi107 – 1115
    Helixi118 – 13114
    Helixi132 – 1343
    Turni138 – 1403
    Helixi144 – 1496
    Helixi158 – 17013
    Helixi174 – 18310
    Helixi191 – 20010

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BDXX-ray6.00A/B/C/D1-203[»]
    1C7YX-ray3.10A1-203[»]
    1CUKX-ray1.90A1-203[»]
    1D8LX-ray2.50A/B1-149[»]
    1HJPX-ray2.50A1-203[»]
    ProteinModelPortaliP0A809.
    SMRiP0A809. Positions 1-203.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A809.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0632.
    HOGENOMiHOG000057116.
    KOiK03550.
    OMAiGYKPQQA.
    OrthoDBiEOG679THG.
    PhylomeDBiP0A809.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    HAMAPiMF_00031. DNA_helic_RuvA.
    InterProiIPR011114. DNA_helicas_Holl-junc_RuvA_C.
    IPR013849. DNA_helicase_Holl-junc_RuvA_I.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR012340. NA-bd_OB-fold.
    IPR000085. RuvA.
    IPR010994. RuvA_2-like.
    [Graphical view]
    PfamiPF07499. RuvA_C. 1 hit.
    PF01330. RuvA_N. 1 hit.
    [Graphical view]
    SMARTiSM00278. HhH1. 2 hits.
    [Graphical view]
    SUPFAMiSSF46929. SSF46929. 1 hit.
    SSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00084. ruvA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A809-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIGRLRGIII EKQPPLVLIE VGGVGYEVHM PMTCFYELPE AGQEAIVFTH    50
    FVVREDAQLL YGFNNKQERT LFKELIKTNG VGPKLALAIL SGMSAQQFVN 100
    AVEREEVGAL VKLPGIGKKT AERLIVEMKD RFKGLHGDLF TPAADLVLTS 150
    PASPATDDAE QEAVAALVAL GYKPQEASRM VSKIARPDAS SETLIREALR 200
    AAL 203
    Length:203
    Mass (Da):22,086
    Last modified:June 7, 2005 - v1
    Checksum:i805DDB79DC5A8385
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti166 – 1661A → R in CAA30119. (PubMed:3279394)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07091 Genomic DNA. Translation: CAA30119.1.
    M21298 Genomic DNA. Translation: AAA24612.1.
    U00096 Genomic DNA. Translation: AAC74931.1.
    AP009048 Genomic DNA. Translation: BAA15672.1.
    D10165 Genomic DNA. Translation: BAA01034.1.
    PIRiE64948. BVECRV.
    RefSeqiNP_416375.1. NC_000913.3.
    YP_490123.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74931; AAC74931; b1861.
    BAA15672; BAA15672; BAA15672.
    GeneIDi12930344.
    946369.
    KEGGiecj:Y75_p1837.
    eco:b1861.
    PATRICi32119045. VBIEscCol129921_1940.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07091 Genomic DNA. Translation: CAA30119.1 .
    M21298 Genomic DNA. Translation: AAA24612.1 .
    U00096 Genomic DNA. Translation: AAC74931.1 .
    AP009048 Genomic DNA. Translation: BAA15672.1 .
    D10165 Genomic DNA. Translation: BAA01034.1 .
    PIRi E64948. BVECRV.
    RefSeqi NP_416375.1. NC_000913.3.
    YP_490123.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BDX X-ray 6.00 A/B/C/D 1-203 [» ]
    1C7Y X-ray 3.10 A 1-203 [» ]
    1CUK X-ray 1.90 A 1-203 [» ]
    1D8L X-ray 2.50 A/B 1-149 [» ]
    1HJP X-ray 2.50 A 1-203 [» ]
    ProteinModelPortali P0A809.
    SMRi P0A809. Positions 1-203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48064N.
    IntActi P0A809. 7 interactions.
    MINTi MINT-1321234.
    STRINGi 511145.b1861.

    Proteomic databases

    PaxDbi P0A809.
    PRIDEi P0A809.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74931 ; AAC74931 ; b1861 .
    BAA15672 ; BAA15672 ; BAA15672 .
    GeneIDi 12930344.
    946369.
    KEGGi ecj:Y75_p1837.
    eco:b1861.
    PATRICi 32119045. VBIEscCol129921_1940.

    Organism-specific databases

    EchoBASEi EB0916.
    EcoGenei EG10923. ruvA.

    Phylogenomic databases

    eggNOGi COG0632.
    HOGENOMi HOG000057116.
    KOi K03550.
    OMAi GYKPQQA.
    OrthoDBi EOG679THG.
    PhylomeDBi P0A809.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10923-MONOMER.
    ECOL316407:JW1850-MONOMER.
    MetaCyc:EG10923-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A809.
    PROi P0A809.

    Gene expression databases

    Genevestigatori P0A809.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    HAMAPi MF_00031. DNA_helic_RuvA.
    InterProi IPR011114. DNA_helicas_Holl-junc_RuvA_C.
    IPR013849. DNA_helicase_Holl-junc_RuvA_I.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR012340. NA-bd_OB-fold.
    IPR000085. RuvA.
    IPR010994. RuvA_2-like.
    [Graphical view ]
    Pfami PF07499. RuvA_C. 1 hit.
    PF01330. RuvA_N. 1 hit.
    [Graphical view ]
    SMARTi SM00278. HhH1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF46929. SSF46929. 1 hit.
    SSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00084. ruvA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes."
      Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G.
      Nucleic Acids Res. 16:1541-1549(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: K12.
    2. "Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination."
      Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.
      J. Bacteriol. 170:4322-4329(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease."
      Takahagi M., Iwasaki H., Nakata A., Shinagawa H.
      J. Bacteriol. 173:5747-5753(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
    7. "RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro."
      Tsaneva I.R., Mueller B., West S.C.
      Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    8. Cited for: SUBCELLULAR LOCATION.
      Strain: K12.
    9. "Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction."
      Rafferty J.B., Sedelnikova S.E., Hargreaves D., Artymiuk P.J., Baker P.J., Sharples G.J., Mahdi A.A., Lloyd R.G., Rice D.W.
      Science 274:415-421(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT.
    10. "Crystal structure of E.coli RuvA with bound DNA Holliday junction at 6-A resolution."
      Hargreaves D., Rice D.W., Sedelnikova S.E., Artymiuk P.J., Lloyd R.G., Rafferty J.B.
      Nat. Struct. Biol. 5:441-446(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (6.0 ANGSTROMS).
    11. "Functional analyses of the domain structure in the Holliday junction binding protein RuvA."
      Nishino T., Ariyoshi M., Iwasaki H., Shinagawa H., Morikawa K.
      Structure 6:11-21(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SUBUNIT.
    12. "Modulation of RuvB function by the mobile domain III of the Holliday junction recognition protein RuvA."
      Nishino T., Iwasaki H., Kataoka M., Ariyoshi M., Fujita T., Shinagawa H., Morikawa K.
      J. Mol. Biol. 298:407-416(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-149.
    13. "Processing of recombination intermediates by the RuvABC proteins."
      West S.C.
      Annu. Rev. Genet. 31:213-244(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRUVA_ECOLI
    AccessioniPrimary (citable) accession number: P0A809
    Secondary accession number(s): P08576
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3