Reviewed, UniProtKB/Swiss-Prot P0A805 (RRF_ECOLI)

Names and origin

Protein names

Recommended name:
    Ribosome-recycling factor
      Short name=RRF
Alternative name(s):
    Ribosome-releasing factor

Gene names

Name:	frr
Synonyms:	rrf
Ordered Locus Names:	b0172, JW0167

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. HAMAP MF_00040
Subcellular location	Cytoplasm. HAMAP MF_00040
Sequence similarities	Belongs to the RRF family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translational termination Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 185

185

Ribosome-recycling factor HAMAP MF_00040

PRO_0000167454

Amino acid modifications

Modified residue

162

1

N6-acetyllysine Ref.12

Secondary structure

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185

Helix Strand Turn

Details...

Helix

2 – 25

24

Turn

34 – 39

6

Beta strand

41 – 44

4

Beta strand

47 – 50

4

Helix

51 – 53

3

Beta strand

55 – 61

7

Beta strand

64 – 71

8

Helix

72 – 74

3

Helix

75 – 84

10

Beta strand

92 – 94

3

Beta strand

97 – 101

5

Helix

107 – 145

39

Helix

150 – 182

33

Sequences

	Sequence		Length	Mass (Da)
	P0A805-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: CBFB9675AAD0CFD8	FASTA	185	20,639
	10 20 30 40 50 60 MISDIRKDAE VRMDKCVEAF KTQISKIRTG RASPSLLDGI VVEYYGTPTP LRQLASVTVE 70 80 90 100 110 120 DSRTLKINVF DRSMSPAVEK AIMASDLGLN PNSAGSDIRV PLPPLTEERR KDLTKIVRGE 130 140 150 160 170 180 AEQARVAVRN VRRDANDKVK ALLKDKEISE DDDRRSQDDV QKLTDAAIKK IEAALADKEA ELMQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of ribosome releasing factor." Ichikawa S., Kaji A. J. Biol. Chem. 264:20054-20059(1989) [PubMed: 2684966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: MRE-600.
[2]	"Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli." Yamanaka K., Ogura T., Niki H., Hiraga S. J. Bacteriol. 174:7517-7526(1992) [PubMed: 1447125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[9]	Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F. Submitted (SEP-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[10]	"Identification of the promoter region of the ribosome-releasing factor cistron (frr)." Shimizu I., Kaji A. J. Bacteriol. 173:5181-5187(1991) [PubMed: 1860827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
[11]	"Ribosome recycling factor (ribosome releasing factor) is essential for bacterial growth." Janosi L., Shimizu I., Kaji A. Proc. Natl. Acad. Sci. U.S.A. 91:4249-4253(1994) [PubMed: 8183897] [Abstract] Cited for: CHARACTERIZATION.
[12]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 8:215-225(2009) [PubMed: 18723842] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-162, MASS SPECTROMETRY.
[13]	"Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications." Agrawal R.K., Sharma M.R., Kiel M.C., Hirokawa G., Booth T.M., Spahn C.M., Grassucci R.A., Kaji A., Frank J. Proc. Natl. Acad. Sci. U.S.A. 101:8900-8905(2004) [PubMed: 15178758] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J05113 Genomic DNA. Translation: AAA24607.1.
D13334 Genomic DNA. Translation: BAA02599.1.
U70214 Genomic DNA. Translation: AAB08601.1.
U00096 Genomic DNA. Translation: AAC73283.1.
AP009048 Genomic DNA. Translation: BAB96748.1.
M69029 Genomic DNA. No translation available.

PIR

A34495.

RefSeq

AP_000832.1.
NP_414714.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EK8	X-ray	2.30	A	1-185	[»]
1ISE	X-ray	2.20	A	1-185	[»]
1ZN0	electron microscopy	15.50	A	1-185	[»]
1ZN1	electron microscopy	14.10	A	1-185	[»]
2RDO	electron microscopy	9.10	8	1-185	[»]

ModBase

Search...

2-D gel databases

SWISS-2DPAGE

P0A805.

Genome annotation databases

GeneID

946122.

GenomeReviews

Gene locus JW0167 in contig AP009048_GR.
Gene locus b0172 in contig U00096_GR.

KEGG

ecj:JW0167.
eco:b0172.

Organism-specific databases

EchoBASE

EB0331.

EcoGene

EG10335. frr.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A805.

OMA

P0A805. NVRRDGM.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10335-MON.

Family and domain databases

HAMAP

MF_00040.
[Tree]

InterPro

IPR002661. Ribosome_recyc_fac.
IPR015998. Ribosome_recycl_fac_bac-like.
[Graphical view]

PANTHER

PTHR20982. Ribosome_recyc_fac. 1 hit.
PTHR20982:SF3. RRF. 1 hit.

Pfam

PF01765. RRF. 1 hit.
[Graphical view]

ProDom

PD004103. RRF. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00496. frr. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RRF_ECOLI

Accession

Primary (citable) accession number: P0A805
Secondary accession number(s): P16174

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)