P0A805 (RRF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome-recycling factor
Short name=RRF

Alternative name(s):
Ribosome-releasing factor

Gene names

Name:	frr
Synonyms:	rrf
Ordered Locus Names:	b0172, JW0167

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. HAMAP MF_00040
Subcellular location	Cytoplasm HAMAP MF_00040.
Sequence similarities	Belongs to the RRF family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 185

185

Ribosome-recycling factor HAMAP MF_00040

PRO_0000167454

Amino acid modifications

Modified residue

162

1

N6-acetyllysine Ref.12

Secondary structure

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185

Helix Strand Turn

Details...

Helix

2 – 25

24

Turn

34 – 39

6

Beta strand

41 – 44

4

Beta strand

47 – 50

4

Helix

51 – 53

3

Beta strand

55 – 61

7

Beta strand

64 – 71

8

Helix

72 – 74

3

Helix

75 – 84

10

Beta strand

92 – 94

3

Beta strand

97 – 101

5

Helix

107 – 145

39

Helix

150 – 182

33

Sequences

	Sequence		Length	Mass (Da)
	P0A805 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: CBFB9675AAD0CFD8	FASTA	185	20,639
	10 20 30 40 50 60 MISDIRKDAE VRMDKCVEAF KTQISKIRTG RASPSLLDGI VVEYYGTPTP LRQLASVTVE 70 80 90 100 110 120 DSRTLKINVF DRSMSPAVEK AIMASDLGLN PNSAGSDIRV PLPPLTEERR KDLTKIVRGE 130 140 150 160 170 180 AEQARVAVRN VRRDANDKVK ALLKDKEISE DDDRRSQDDV QKLTDAAIKK IEAALADKEA ELMQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of ribosome releasing factor." Ichikawa S., Kaji A. J. Biol. Chem. 264:20054-20059(1989) [PubMed: 2684966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: MRE-600.
[2]	"Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli." Yamanaka K., Ogura T., Niki H., Hiraga S. J. Bacteriol. 174:7517-7526(1992) [PubMed: 1447125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[9]	Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F. Submitted (SEP-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[10]	"Identification of the promoter region of the ribosome-releasing factor cistron (frr)." Shimizu I., Kaji A. J. Bacteriol. 173:5181-5187(1991) [PubMed: 1860827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
[11]	"Ribosome recycling factor (ribosome releasing factor) is essential for bacterial growth." Janosi L., Shimizu I., Kaji A. Proc. Natl. Acad. Sci. U.S.A. 91:4249-4253(1994) [PubMed: 8183897] [Abstract] Cited for: CHARACTERIZATION.
[12]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 8:215-225(2009) [PubMed: 18723842] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-162, MASS SPECTROMETRY. Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.
[13]	"Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications." Agrawal R.K., Sharma M.R., Kiel M.C., Hirokawa G., Booth T.M., Spahn C.M., Grassucci R.A., Kaji A., Frank J. Proc. Natl. Acad. Sci. U.S.A. 101:8900-8905(2004) [PubMed: 15178758] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J05113 Genomic DNA. Translation: AAA24607.1.
D13334 Genomic DNA. Translation: BAA02599.1.
U70214 Genomic DNA. Translation: AAB08601.1.
U00096 Genomic DNA. Translation: AAC73283.1.
AP009048 Genomic DNA. Translation: BAB96748.1.
M69029 Genomic DNA. No translation available.

PIR

A34495.

RefSeq

NP_414714.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EK8	X-ray	2.30	A	1-185	[»]
1ISE	X-ray	2.20	A	1-185	[»]
1ZN0	electron microscopy	15.50	A	1-185	[»]
1ZN1	electron microscopy	14.10	A	1-185	[»]
2RDO	electron microscopy	9.10	8	1-185	[»]
3R8N	X-ray	3.00	Y	2-183	[»]

ProteinModelPortal

P0A805.

SMR

P0A805. Positions 1-185.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48120N.

IntAct

P0A805. 4 interactions.

MINT

MINT-1308979.

2D gel databases

SWISS-2DPAGE

P0A805.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001100; EBESCP00000001100; EBESCG00000000913.
EBESCT00000016070; EBESCP00000015361; EBESCG00000015130.

GeneID

946122.

GenomeReviews

Gene locus JW0167 in contig AP009048_GR.
Gene locus b0172 in contig U00096_GR.

KEGG

ecj:JW0167.
eco:b0172.

PATRIC

32115453. VBIEscCol129921_0178.

Organism-specific databases

EchoBASE

EB0331.

EcoGene

EG10335. frr.

Phylogenomic databases

eggNOG

COG0233.

GeneTree

EBGT00050000009395.

HOGENOM

HBG533975.

OMA

IRLNIPP.

PhylomeDB

P0A805.

ProtClustDB

PRK00083.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10335-MONOMER.

Gene expression databases

Genevestigator

P0A805.

Family and domain databases

HAMAP

MF_00040. RRF.
[Tree]

InterPro

IPR002661. Ribosome_recyc_fac.
IPR023584. Ribosome_recyc_fac_dom.
[Graphical view]

KO

K02838.

PANTHER

PTHR20982. Ribosome_recyc_fac. 1 hit.

Pfam

PF01765. RRF. 1 hit.
[Graphical view]

SUPFAM

SSF55194. Ribosome_recyc_fac. 1 hit.

TIGRFAMs

TIGR00496. Frr. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RRF_ECOLI

Accession

Primary (citable) accession number: P0A805
Secondary accession number(s): P16174

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program