Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0A800

- RPOZ_ECOLI

UniProt

P0A800 - RPOZ_ECOLI

Protein

DNA-directed RNA polymerase subunit omega

Gene

rpoZ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP
    3. protein binding Source: EcoCyc

    GO - Biological processi

    1. protein complex assembly Source: EcoCyc
    2. transcription, DNA-templated Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Transcription

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10899-MONOMER.
    ECOL316407:JW3624-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase subunit omega (EC:2.7.7.6)
    Short name:
    RNAP omega subunit
    Alternative name(s):
    RNA polymerase omega subunit
    Transcriptase subunit omega
    Gene namesi
    Name:rpoZ
    Ordered Locus Names:b3649, JW3624
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10899. rpoZ.

    Subcellular locationi

    GO - Cellular componenti

    1. RNA polymerase complex Source: EcoliWiki

    Keywords - Cellular componenti

    DNA-directed RNA polymerase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 9190DNA-directed RNA polymerase subunit omegaPRO_0000128935Add
    BLAST

    Proteomic databases

    PaxDbiP0A800.
    PRIDEiP0A800.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A800.

    Interactioni

    Subunit structurei

    The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.1 Publication

    Protein-protein interaction databases

    DIPiDIP-31837N.
    IntActiP0A800. 26 interactions.
    MINTiMINT-1222720.
    STRINGi511145.b3649.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IYDelectron microscopy-E2-91[»]
    3LU0electron microscopy-E1-91[»]
    4IGCX-ray3.70E/J1-91[»]
    4JK1X-ray3.90E/J1-91[»]
    4JK2X-ray4.20E/J1-91[»]
    4KMUX-ray3.85E/J1-91[»]
    4KN4X-ray3.96E/J1-91[»]
    4KN7X-ray3.69E/J1-91[»]
    4MEXX-ray3.90E/K1-91[»]
    4MEYX-ray3.95E/K1-91[»]
    ProteinModelPortaliP0A800.
    SMRiP0A800. Positions 2-90.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A800.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1758.
    HOGENOMiHOG000245721.
    KOiK03060.
    OMAiIMNAQER.
    OrthoDBiEOG6Q5NV5.
    PhylomeDBiP0A800.

    Family and domain databases

    Gene3Di3.90.940.10. 1 hit.
    HAMAPiMF_00366. RNApol_bact_RpoZ.
    InterProiIPR003716. DNA-dir_RNA_pol_omega.
    IPR006110. Pol_omega/K/RPB6.
    IPR012293. RNAP_RPB6_omega.
    [Graphical view]
    PfamiPF01192. RNA_pol_Rpb6. 1 hit.
    [Graphical view]
    SUPFAMiSSF63562. SSF63562. 1 hit.
    TIGRFAMsiTIGR00690. rpoZ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A800-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARVTVQDAV EKIGNRFDLV LVAARRARQM QVGGKDPLVP EENDKTTVIA   50
    LREIEEGLIN NQILDVRERQ EQQEQEAAEL QAVTAIAEGR R 91
    Length:91
    Mass (Da):10,237
    Last modified:August 1, 1988 - v1
    Checksum:i4DE7E81EF670D887
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24503 Genomic DNA. Translation: AAB00159.1.
    M15266 Genomic DNA. Translation: AAA24229.1.
    L10328 Genomic DNA. Translation: AAA62002.1.
    U00096 Genomic DNA. Translation: AAC76673.1.
    AP009048 Genomic DNA. Translation: BAE77644.1.
    PIRiA29038. RNECO.
    RefSeqiNP_418106.1. NC_000913.3.
    YP_491785.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76673; AAC76673; b3649.
    BAE77644; BAE77644; BAE77644.
    GeneIDi12930353.
    948160.
    KEGGiecj:Y75_p3525.
    eco:b3649.
    PATRICi32122785. VBIEscCol129921_3769.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24503 Genomic DNA. Translation: AAB00159.1 .
    M15266 Genomic DNA. Translation: AAA24229.1 .
    L10328 Genomic DNA. Translation: AAA62002.1 .
    U00096 Genomic DNA. Translation: AAC76673.1 .
    AP009048 Genomic DNA. Translation: BAE77644.1 .
    PIRi A29038. RNECO.
    RefSeqi NP_418106.1. NC_000913.3.
    YP_491785.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IYD electron microscopy - E 2-91 [» ]
    3LU0 electron microscopy - E 1-91 [» ]
    4IGC X-ray 3.70 E/J 1-91 [» ]
    4JK1 X-ray 3.90 E/J 1-91 [» ]
    4JK2 X-ray 4.20 E/J 1-91 [» ]
    4KMU X-ray 3.85 E/J 1-91 [» ]
    4KN4 X-ray 3.96 E/J 1-91 [» ]
    4KN7 X-ray 3.69 E/J 1-91 [» ]
    4MEX X-ray 3.90 E/K 1-91 [» ]
    4MEY X-ray 3.95 E/K 1-91 [» ]
    ProteinModelPortali P0A800.
    SMRi P0A800. Positions 2-90.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-31837N.
    IntActi P0A800. 26 interactions.
    MINTi MINT-1222720.
    STRINGi 511145.b3649.

    Chemistry

    ChEMBLi CHEMBL2364672.

    Proteomic databases

    PaxDbi P0A800.
    PRIDEi P0A800.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76673 ; AAC76673 ; b3649 .
    BAE77644 ; BAE77644 ; BAE77644 .
    GeneIDi 12930353.
    948160.
    KEGGi ecj:Y75_p3525.
    eco:b3649.
    PATRICi 32122785. VBIEscCol129921_3769.

    Organism-specific databases

    EchoBASEi EB0892.
    EcoGenei EG10899. rpoZ.

    Phylogenomic databases

    eggNOGi COG1758.
    HOGENOMi HOG000245721.
    KOi K03060.
    OMAi IMNAQER.
    OrthoDBi EOG6Q5NV5.
    PhylomeDBi P0A800.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10899-MONOMER.
    ECOL316407:JW3624-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A800.
    PROi P0A800.

    Gene expression databases

    Genevestigatori P0A800.

    Family and domain databases

    Gene3Di 3.90.940.10. 1 hit.
    HAMAPi MF_00366. RNApol_bact_RpoZ.
    InterProi IPR003716. DNA-dir_RNA_pol_omega.
    IPR006110. Pol_omega/K/RPB6.
    IPR012293. RNAP_RPB6_omega.
    [Graphical view ]
    Pfami PF01192. RNA_pol_Rpb6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63562. SSF63562. 1 hit.
    TIGRFAMsi TIGR00690. rpoZ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The cloning and sequence of the gene encoding the omega subunit of Escherichia coli RNA polymerase."
      Gentry D.R., Burgess R.R.
      Gene 48:33-40(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization of the spoT gene of Escherichia coli."
      Sarubbi E., Rudd K.E., Xiao H., Ikehara K., Kalman M., Cashel M.
      J. Biol. Chem. 264:15074-15082(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / JM109 / ATCC 53323.
    3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
      Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
      Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Small genes/gene-products in Escherichia coli K-12."
      Wasinger V.C., Humphery-Smith I.
      FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11.
      Strain: K12.
    7. "Three-dimensional EM structure of an intact activator-dependent transcription initiation complex."
      Hudson B.P., Quispe J., Lara-Gonzalez S., Kim Y., Berman H.M., Arnold E., Ebright R.H., Lawson C.L.
      Proc. Natl. Acad. Sci. U.S.A. 106:19830-19835(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (19.80 ANGSTROMS) IN COMPLEX WITH RPOA; RPOB; RPOC; RPOD; CRP AND DNA, DNA-BINDING, SUBUNIT.

    Entry informationi

    Entry nameiRPOZ_ECOLI
    AccessioniPrimary (citable) accession number: P0A800
    Secondary accession number(s): P08374, Q2M7W2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3