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P0A800

- RPOZ_ECOLI

UniProt

P0A800 - RPOZ_ECOLI

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Protein

DNA-directed RNA polymerase subunit omega

Gene

rpoZ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein complex assembly Source: EcoCyc
  2. transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciEcoCyc:EG10899-MONOMER.
ECOL316407:JW3624-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit omega (EC:2.7.7.6)
Short name:
RNAP omega subunit
Alternative name(s):
RNA polymerase omega subunit
Transcriptase subunit omega
Gene namesi
Name:rpoZ
Ordered Locus Names:b3649, JW3624
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10899. rpoZ.

Subcellular locationi

GO - Cellular componenti

  1. RNA polymerase complex Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 9190DNA-directed RNA polymerase subunit omegaPRO_0000128935Add
BLAST

Proteomic databases

PaxDbiP0A800.
PRIDEiP0A800.

Expressioni

Gene expression databases

GenevestigatoriP0A800.

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.1 Publication

Protein-protein interaction databases

DIPiDIP-31837N.
IntActiP0A800. 26 interactions.
MINTiMINT-1222720.
STRINGi511145.b3649.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IYDelectron microscopy-E2-91[»]
3LU0electron microscopy-E1-91[»]
4IGCX-ray3.70E/J1-91[»]
4JK1X-ray3.90E/J1-91[»]
4JK2X-ray4.20E/J1-91[»]
4KMUX-ray3.85E/J1-91[»]
4KN4X-ray3.96E/J1-91[»]
4KN7X-ray3.69E/J1-91[»]
4MEXX-ray3.90E/K1-91[»]
4MEYX-ray3.95E/K1-91[»]
ProteinModelPortaliP0A800.
SMRiP0A800. Positions 2-90.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A800.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1758.
HOGENOMiHOG000245721.
InParanoidiP0A800.
KOiK03060.
OMAiIMNAQER.
OrthoDBiEOG6Q5NV5.
PhylomeDBiP0A800.

Family and domain databases

Gene3Di3.90.940.10. 1 hit.
HAMAPiMF_00366. RNApol_bact_RpoZ.
InterProiIPR003716. DNA-dir_RNA_pol_omega.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
[Graphical view]
PfamiPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMiSSF63562. SSF63562. 1 hit.
TIGRFAMsiTIGR00690. rpoZ. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A800-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARVTVQDAV EKIGNRFDLV LVAARRARQM QVGGKDPLVP EENDKTTVIA
60 70 80 90
LREIEEGLIN NQILDVRERQ EQQEQEAAEL QAVTAIAEGR R
Length:91
Mass (Da):10,237
Last modified:August 1, 1988 - v1
Checksum:i4DE7E81EF670D887
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24503 Genomic DNA. Translation: AAB00159.1.
M15266 Genomic DNA. Translation: AAA24229.1.
L10328 Genomic DNA. Translation: AAA62002.1.
U00096 Genomic DNA. Translation: AAC76673.1.
AP009048 Genomic DNA. Translation: BAE77644.1.
PIRiA29038. RNECO.
RefSeqiNP_418106.1. NC_000913.3.
YP_491785.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76673; AAC76673; b3649.
BAE77644; BAE77644; BAE77644.
GeneIDi12930353.
948160.
KEGGiecj:Y75_p3525.
eco:b3649.
PATRICi32122785. VBIEscCol129921_3769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24503 Genomic DNA. Translation: AAB00159.1 .
M15266 Genomic DNA. Translation: AAA24229.1 .
L10328 Genomic DNA. Translation: AAA62002.1 .
U00096 Genomic DNA. Translation: AAC76673.1 .
AP009048 Genomic DNA. Translation: BAE77644.1 .
PIRi A29038. RNECO.
RefSeqi NP_418106.1. NC_000913.3.
YP_491785.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3IYD electron microscopy - E 2-91 [» ]
3LU0 electron microscopy - E 1-91 [» ]
4IGC X-ray 3.70 E/J 1-91 [» ]
4JK1 X-ray 3.90 E/J 1-91 [» ]
4JK2 X-ray 4.20 E/J 1-91 [» ]
4KMU X-ray 3.85 E/J 1-91 [» ]
4KN4 X-ray 3.96 E/J 1-91 [» ]
4KN7 X-ray 3.69 E/J 1-91 [» ]
4MEX X-ray 3.90 E/K 1-91 [» ]
4MEY X-ray 3.95 E/K 1-91 [» ]
ProteinModelPortali P0A800.
SMRi P0A800. Positions 2-90.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-31837N.
IntActi P0A800. 26 interactions.
MINTi MINT-1222720.
STRINGi 511145.b3649.

Chemistry

ChEMBLi CHEMBL2364672.

Proteomic databases

PaxDbi P0A800.
PRIDEi P0A800.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76673 ; AAC76673 ; b3649 .
BAE77644 ; BAE77644 ; BAE77644 .
GeneIDi 12930353.
948160.
KEGGi ecj:Y75_p3525.
eco:b3649.
PATRICi 32122785. VBIEscCol129921_3769.

Organism-specific databases

EchoBASEi EB0892.
EcoGenei EG10899. rpoZ.

Phylogenomic databases

eggNOGi COG1758.
HOGENOMi HOG000245721.
InParanoidi P0A800.
KOi K03060.
OMAi IMNAQER.
OrthoDBi EOG6Q5NV5.
PhylomeDBi P0A800.

Enzyme and pathway databases

BioCyci EcoCyc:EG10899-MONOMER.
ECOL316407:JW3624-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A800.
PROi P0A800.

Gene expression databases

Genevestigatori P0A800.

Family and domain databases

Gene3Di 3.90.940.10. 1 hit.
HAMAPi MF_00366. RNApol_bact_RpoZ.
InterProi IPR003716. DNA-dir_RNA_pol_omega.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
[Graphical view ]
Pfami PF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view ]
SUPFAMi SSF63562. SSF63562. 1 hit.
TIGRFAMsi TIGR00690. rpoZ. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The cloning and sequence of the gene encoding the omega subunit of Escherichia coli RNA polymerase."
    Gentry D.R., Burgess R.R.
    Gene 48:33-40(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of the spoT gene of Escherichia coli."
    Sarubbi E., Rudd K.E., Xiao H., Ikehara K., Kalman M., Cashel M.
    J. Biol. Chem. 264:15074-15082(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / JM109 / ATCC 53323.
  3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Small genes/gene-products in Escherichia coli K-12."
    Wasinger V.C., Humphery-Smith I.
    FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.
    Strain: K12.
  7. "Three-dimensional EM structure of an intact activator-dependent transcription initiation complex."
    Hudson B.P., Quispe J., Lara-Gonzalez S., Kim Y., Berman H.M., Arnold E., Ebright R.H., Lawson C.L.
    Proc. Natl. Acad. Sci. U.S.A. 106:19830-19835(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (19.80 ANGSTROMS) IN COMPLEX WITH RPOA; RPOB; RPOC; RPOD; CRP AND DNA, DNA-BINDING, SUBUNIT.

Entry informationi

Entry nameiRPOZ_ECOLI
AccessioniPrimary (citable) accession number: P0A800
Secondary accession number(s): P08374, Q2M7W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3