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P0A800 (RPOZ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit omega

Short name=RNAP omega subunit
EC=2.7.7.6
Alternative name(s):
RNA polymerase omega subunit
Transcriptase subunit omega
Gene names
Name:rpoZ
Ordered Locus Names:b3649, JW3624
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length91 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. HAMAP-Rule MF_00366

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00366

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. Ref.7

Sequence similarities

Belongs to the RNA polymerase subunit omega family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 9190DNA-directed RNA polymerase subunit omega HAMAP-Rule MF_00366
PRO_0000128935

Sequences

Sequence LengthMass (Da)Tools
P0A800 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 4DE7E81EF670D887

FASTA9110,237
        10         20         30         40         50         60 
MARVTVQDAV EKIGNRFDLV LVAARRARQM QVGGKDPLVP EENDKTTVIA LREIEEGLIN 

        70         80         90 
NQILDVRERQ EQQEQEAAEL QAVTAIAEGR R 

« Hide

References

« Hide 'large scale' references
[1]"The cloning and sequence of the gene encoding the omega subunit of Escherichia coli RNA polymerase."
Gentry D.R., Burgess R.R.
Gene 48:33-40(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Characterization of the spoT gene of Escherichia coli."
Sarubbi E., Rudd K.E., Xiao H., Ikehara K., Kalman M., Cashel M.
J. Biol. Chem. 264:15074-15082(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / JM109 / ATCC 53323.
[3]"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Small genes/gene-products in Escherichia coli K-12."
Wasinger V.C., Humphery-Smith I.
FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11.
Strain: K12.
[7]"Three-dimensional EM structure of an intact activator-dependent transcription initiation complex."
Hudson B.P., Quispe J., Lara-Gonzalez S., Kim Y., Berman H.M., Arnold E., Ebright R.H., Lawson C.L.
Proc. Natl. Acad. Sci. U.S.A. 106:19830-19835(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (19.80 ANGSTROMS) IN COMPLEX WITH RPOA; RPOB; RPOC; RPOD; CRP AND DNA, DNA-BINDING, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24503 Genomic DNA. Translation: AAB00159.1.
M15266 Genomic DNA. Translation: AAA24229.1.
L10328 Genomic DNA. Translation: AAA62002.1.
U00096 Genomic DNA. Translation: AAC76673.1.
AP009048 Genomic DNA. Translation: BAE77644.1.
PIRRNECO. A29038.
RefSeqNP_418106.1. NC_000913.3.
YP_491785.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IYDelectron microscopy-E2-91[»]
3LU0electron microscopy-E1-91[»]
4IGCX-ray3.70E/J1-91[»]
4JK1X-ray3.90E/J1-91[»]
4JK2X-ray4.20E/J1-91[»]
4KMUX-ray3.85E/J1-91[»]
4KN4X-ray3.96E/J1-91[»]
4KN7X-ray3.69E/J1-91[»]
4MEXX-ray3.90E/K1-91[»]
4MEYX-ray3.95E/K1-91[»]
ProteinModelPortalP0A800.
SMRP0A800. Positions 2-90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-31837N.
IntActP0A800. 26 interactions.
MINTMINT-1222720.
STRING511145.b3649.

Chemistry

ChEMBLCHEMBL2364672.

Proteomic databases

PaxDbP0A800.
PRIDEP0A800.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76673; AAC76673; b3649.
BAE77644; BAE77644; BAE77644.
GeneID12930353.
948160.
KEGGecj:Y75_p3525.
eco:b3649.
PATRIC32122785. VBIEscCol129921_3769.

Organism-specific databases

EchoBASEEB0892.
EcoGeneEG10899. rpoZ.

Phylogenomic databases

eggNOGCOG1758.
HOGENOMHOG000245721.
KOK03060.
OMAIMNAQER.
OrthoDBEOG6Q5NV5.
PhylomeDBP0A800.

Enzyme and pathway databases

BioCycEcoCyc:EG10899-MONOMER.
ECOL316407:JW3624-MONOMER.

Gene expression databases

GenevestigatorP0A800.

Family and domain databases

Gene3D3.90.940.10. 1 hit.
HAMAPMF_00366. RNApol_bact_RpoZ.
InterProIPR003716. DNA-dir_RNA_pol_omega.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
[Graphical view]
PfamPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMSSF63562. SSF63562. 1 hit.
TIGRFAMsTIGR00690. rpoZ. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A800.
PROP0A800.

Entry information

Entry nameRPOZ_ECOLI
AccessionPrimary (citable) accession number: P0A800
Secondary accession number(s): P08374, Q2M7W2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene