P0A7Z0 (RPIA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-5-phosphate isomerase A
EC=5.3.1.6

Alternative name(s):
Phosphoriboisomerase A
Short name=PRI

Gene names

Name:	rpiA
Synonyms:	ygfC
Ordered Locus Names:	b2914, JW5475

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-ribose 5-phosphate = D-ribulose 5-phosphate. HAMAP MF_00170
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1. HAMAP MF_00170
Subunit structure	Homodimer.
Sequence similarities	Belongs to the ribose 5-phosphate isomerase family.
Sequence caution	The sequence CAA47309.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	pentose-phosphate shunt, non-oxidative branch Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	ribose-5-phosphate isomerase activity Inferred from direct assay Ref.1. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 219

219

Ribose-5-phosphate isomerase A HAMAP MF_00170

PRO_0000158412

Secondary structure

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219

Helix Strand Turn

Details...

Helix

3 – 13

11

Helix

14 – 16

3

Beta strand

24 – 26

3

Beta strand

49 – 52

4

Helix

70 – 72

3

Beta strand

76 – 81

6

Beta strand

84 – 86

3

Helix

100 – 109

10

Beta strand

110 – 118

9

Helix

119 – 121

3

Beta strand

124 – 126

3

Beta strand

131 – 135

5

Helix

137 – 139

3

Helix

140 – 149

10

Beta strand

153 – 156

4

Beta strand

167 – 173

7

Helix

179 – 187

9

Beta strand

192 – 198

7

Beta strand

204 – 210

7

Beta strand

213 – 217

5

Sequences

	Sequence		Length	Mass (Da)
	P0A7Z0 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: B53C49CC3DB188BC	FASTA	219	22,860
	10 20 30 40 50 60 MTQDELKKAV GWAALQYVQP GTIVGVGTGS TAAHFIDALG TMKGQIEGAV SSSDASTEKL 70 80 90 100 110 120 KSLGIHVFDL NEVDSLGIYV DGADEINGHM QMIKGGGAAL TREKIIASVA EKFICIADAS 130 140 150 160 170 180 KQVDILGKFP LPVEVIPMAR SAVARQLVKL GGRPEYRQGV VTDNGNVILD VHGMEILDPI 190 200 210 AMENAINAIP GVVTVGLFAN RGADVALIGT PDGVKTIVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli rpiA gene encoding ribose phosphate isomerase A." Hove-Jensen B., Maigaard M. J. Bacteriol. 175:5628-5635(1993) [PubMed: 8366047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: K12.
[2]	Roy I., Leadlay P.F. Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The tdh and serA operons of Escherichia coli: mutational analysis of the regulatory elements of leucine-responsive genes." Rex J.H., Aronson B.D., Somerville R.L. J. Bacteriol. 173:5944-5953(1991) [PubMed: 1917830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 154-219. Strain: K12.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[7]	"Protein identification with N and C-terminal sequence tags in proteome projects." Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F. J. Mol. Biol. 278:599-608(1998) [PubMed: 9600841] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-4. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X73026 Genomic DNA. Translation: CAA51509.1.
X66836 Genomic DNA. Translation: CAA47309.1. Different initiation.
U28377 Genomic DNA. Translation: AAA69081.1.
U00096 Genomic DNA. Translation: AAC75951.1.
AP009048 Genomic DNA. Translation: BAE76978.1.
M64630 Genomic DNA. Translation: AAA73015.1.

PIR

A65076.

RefSeq

NP_417389.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KS2	X-ray	1.50	A/B	1-219	[»]
1LKZ	X-ray	2.50	A/B	1-219	[»]
1O8B	X-ray	1.25	A/B	1-219	[»]

ProteinModelPortal

P0A7Z0.

SMR

P0A7Z0. Positions 2-219.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7Z0. 1 interaction.

2D gel databases

SWISS-2DPAGE

P0A7Z0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004984; EBESCP00000004984; EBESCG00000004069.
EBESCT00000017127; EBESCP00000016418; EBESCG00000016186.

GeneID

947407.

GenomeReviews

Gene locus JW5475 in contig AP009048_GR.
Gene locus b2914 in contig U00096_GR.

KEGG

ecj:JW5475.
eco:b2914.

PATRIC

32121242. VBIEscCol129921_3009.

Organism-specific databases

EchoBASE

EB1413.

EcoGene

EG11443. rpiA.

Phylogenomic databases

eggNOG

COG0120.

GeneTree

EBGT00050000011820.

HOGENOM

HBG515603.

OMA

GGDPEYR.

PhylomeDB

P0A7Z0.

ProtClustDB

PRK00702.

Enzyme and pathway databases

BioCyc

EcoCyc:RIB5PISOMA-MONOMER.
MetaCyc:RIB5PISOMA-MONOMER.

Gene expression databases

Genevestigator

P0A7Z0.

Family and domain databases

HAMAP

MF_00170. Rib_5P_isom_A.
[Tree]

InterPro

IPR004788. Ribose5P_isomerase_typA.
IPR020672. Ribose5P_isomerase_typA_subgr.
[Graphical view]

KO

K01807.

PANTHER

PTHR11934. RpiA. 1 hit.

Pfam

PF06026. Rib_5-P_isom_A. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00021. RpiA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RPIA_ECOLI

Accession

Primary (citable) accession number: P0A7Z0
Secondary accession number(s): P27252, Q2M9S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program