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Protein

Ribonuclease P protein component

Gene

rnpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.UniRule annotation1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation1 Publication

GO - Molecular functioni

  • ribonuclease P activity Source: EcoCyc
  • tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  • RNA processing Source: EcoliWiki
  • tRNA 5'-leader removal Source: EcoliWiki
  • tRNA processing Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10862-MONOMER.
ECOL316407:JW3681-MONOMER.
MetaCyc:EG10862-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein componentUniRule annotation (EC:3.1.26.5UniRule annotation)
Short name:
RNase P proteinUniRule annotation
Short name:
RNaseP proteinUniRule annotation
Alternative name(s):
Protein C5UniRule annotation
Gene namesi
Name:rnpAUniRule annotation
Ordered Locus Names:b3704, JW3681
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10862. rnpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 119119Ribonuclease P protein componentPRO_0000198458Add
BLAST

Proteomic databases

PaxDbiP0A7Y8.

Interactioni

Subunit structurei

Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261766. 14 interactions.
DIPiDIP-36039N.
IntActiP0A7Y8. 56 interactions.
MINTiMINT-1218365.
STRINGi511145.b3704.

Structurei

Secondary structure

1
119
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 104Combined sources
Helixi17 – 237Combined sources
Beta strandi33 – 353Combined sources
Turni53 – 553Combined sources
Helixi59 – 7315Combined sources
Turni77 – 793Combined sources
Beta strandi82 – 854Combined sources
Beta strandi87 – 893Combined sources
Helixi93 – 953Combined sources
Helixi98 – 11720Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LJPNMR-A1-119[»]
ProteinModelPortaliP0A7Y8.
SMRiP0A7Y8. Positions 1-119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RnpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NVJ. Bacteria.
COG0594. LUCA.
HOGENOMiHOG000266301.
InParanoidiP0A7Y8.
KOiK03536.
OMAiPPMDFVV.
PhylomeDBiP0A7Y8.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
HAMAPiMF_00227. RNase_P. 1 hit.
InterProiIPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000100. RNase_P.
IPR020539. RNase_P_CS.
[Graphical view]
PfamiPF00825. Ribonuclease_P. 1 hit.
[Graphical view]
ProDomiPD003629. Ribonuclease_P. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR00188. rnpA. 1 hit.
PROSITEiPS00648. RIBONUCLEASE_P. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A7Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT
60 70 80 90 100
VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA
110
LSEALEKLWR RHCRLARGS
Length:119
Mass (Da):13,789
Last modified:January 1, 1988 - v1
Checksum:i449D9EDACA1C6240
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11056 Genomic DNA. Translation: AAA24567.1.
X01861 Genomic DNA. Translation: CAA25983.1.
L10328 Genomic DNA. Translation: AAA62055.1.
U00096 Genomic DNA. Translation: AAC76727.1.
AP009048 Genomic DNA. Translation: BAE77590.1.
PIRiA00794. NRECP.
RefSeqiNP_418159.1. NC_000913.3.
WP_000239730.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76727; AAC76727; b3704.
BAE77590; BAE77590; BAE77590.
GeneIDi948215.
KEGGiecj:JW3681.
eco:b3704.
PATRICi32122905. VBIEscCol129921_3828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11056 Genomic DNA. Translation: AAA24567.1.
X01861 Genomic DNA. Translation: CAA25983.1.
L10328 Genomic DNA. Translation: AAA62055.1.
U00096 Genomic DNA. Translation: AAC76727.1.
AP009048 Genomic DNA. Translation: BAE77590.1.
PIRiA00794. NRECP.
RefSeqiNP_418159.1. NC_000913.3.
WP_000239730.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LJPNMR-A1-119[»]
ProteinModelPortaliP0A7Y8.
SMRiP0A7Y8. Positions 1-119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261766. 14 interactions.
DIPiDIP-36039N.
IntActiP0A7Y8. 56 interactions.
MINTiMINT-1218365.
STRINGi511145.b3704.

Proteomic databases

PaxDbiP0A7Y8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76727; AAC76727; b3704.
BAE77590; BAE77590; BAE77590.
GeneIDi948215.
KEGGiecj:JW3681.
eco:b3704.
PATRICi32122905. VBIEscCol129921_3828.

Organism-specific databases

EchoBASEiEB0855.
EcoGeneiEG10862. rnpA.

Phylogenomic databases

eggNOGiENOG4105NVJ. Bacteria.
COG0594. LUCA.
HOGENOMiHOG000266301.
InParanoidiP0A7Y8.
KOiK03536.
OMAiPPMDFVV.
PhylomeDBiP0A7Y8.

Enzyme and pathway databases

BioCyciEcoCyc:EG10862-MONOMER.
ECOL316407:JW3681-MONOMER.
MetaCyc:EG10862-MONOMER.

Miscellaneous databases

PROiP0A7Y8.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
HAMAPiMF_00227. RNase_P. 1 hit.
InterProiIPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000100. RNase_P.
IPR020539. RNase_P_CS.
[Graphical view]
PfamiPF00825. Ribonuclease_P. 1 hit.
[Graphical view]
ProDomiPD003629. Ribonuclease_P. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR00188. rnpA. 1 hit.
PROSITEiPS00648. RIBONUCLEASE_P. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNPA_ECOLI
AccessioniPrimary (citable) accession number: P0A7Y8
Secondary accession number(s): P06277, Q2M816
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.