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P0A7Y8

- RNPA_ECOLI

UniProt

P0A7Y8 - RNPA_ECOLI

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Protein

Ribonuclease P protein component

Gene

rnpA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.1 PublicationUniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.1 PublicationUniRule annotation

GO - Molecular functioni

  1. ribonuclease P activity Source: EcoCyc
  2. tRNA binding Source: InterPro

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. RNA processing Source: EcoliWiki
  4. tRNA 5'-leader removal Source: EcoliWiki
  5. tRNA processing Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10862-MONOMER.
ECOL316407:JW3681-MONOMER.
MetaCyc:EG10862-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein componentUniRule annotation (EC:3.1.26.5UniRule annotation)
Short name:
RNase P proteinUniRule annotation
Short name:
RNaseP proteinUniRule annotation
Alternative name(s):
Protein C5UniRule annotation
Gene namesi
Name:rnpAUniRule annotation
Ordered Locus Names:b3704, JW3681
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10862. rnpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 119119Ribonuclease P protein componentPRO_0000198458Add
BLAST

Proteomic databases

PaxDbiP0A7Y8.

Expressioni

Gene expression databases

GenevestigatoriP0A7Y8.

Interactioni

Subunit structurei

Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.1 PublicationUniRule annotation

Protein-protein interaction databases

DIPiDIP-36039N.
IntActiP0A7Y8. 56 interactions.
MINTiMINT-1218365.
STRINGi511145.b3704.

Structurei

Secondary structure

1
119
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 104Combined sources
Helixi17 – 237Combined sources
Beta strandi33 – 353Combined sources
Turni53 – 553Combined sources
Helixi59 – 7315Combined sources
Turni77 – 793Combined sources
Beta strandi82 – 854Combined sources
Beta strandi87 – 893Combined sources
Helixi93 – 953Combined sources
Helixi98 – 11720Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LJPNMR-A1-119[»]
ProteinModelPortaliP0A7Y8.
SMRiP0A7Y8. Positions 1-119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RnpA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0594.
HOGENOMiHOG000266301.
InParanoidiP0A7Y8.
KOiK03536.
OMAiPPMDFVV.
OrthoDBiEOG6C01C6.
PhylomeDBiP0A7Y8.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
HAMAPiMF_00227. RNase_P.
InterProiIPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000100. RNase_P.
IPR020539. RNase_P_CS.
[Graphical view]
PfamiPF00825. Ribonuclease_P. 1 hit.
[Graphical view]
ProDomiPD003629. Ribonuclease_P. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR00188. rnpA. 1 hit.
PROSITEiPS00648. RIBONUCLEASE_P. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A7Y8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT
60 70 80 90 100
VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA
110
LSEALEKLWR RHCRLARGS
Length:119
Mass (Da):13,789
Last modified:January 1, 1988 - v1
Checksum:i449D9EDACA1C6240
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11056 Genomic DNA. Translation: AAA24567.1.
X01861 Genomic DNA. Translation: CAA25983.1.
L10328 Genomic DNA. Translation: AAA62055.1.
U00096 Genomic DNA. Translation: AAC76727.1.
AP009048 Genomic DNA. Translation: BAE77590.1.
PIRiA00794. NRECP.
RefSeqiNP_418159.1. NC_000913.3.
YP_491731.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76727; AAC76727; b3704.
BAE77590; BAE77590; BAE77590.
GeneIDi12932772.
948215.
KEGGiecj:Y75_p3469.
eco:b3704.
PATRICi32122905. VBIEscCol129921_3828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11056 Genomic DNA. Translation: AAA24567.1 .
X01861 Genomic DNA. Translation: CAA25983.1 .
L10328 Genomic DNA. Translation: AAA62055.1 .
U00096 Genomic DNA. Translation: AAC76727.1 .
AP009048 Genomic DNA. Translation: BAE77590.1 .
PIRi A00794. NRECP.
RefSeqi NP_418159.1. NC_000913.3.
YP_491731.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LJP NMR - A 1-119 [» ]
ProteinModelPortali P0A7Y8.
SMRi P0A7Y8. Positions 1-119.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36039N.
IntActi P0A7Y8. 56 interactions.
MINTi MINT-1218365.
STRINGi 511145.b3704.

Proteomic databases

PaxDbi P0A7Y8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76727 ; AAC76727 ; b3704 .
BAE77590 ; BAE77590 ; BAE77590 .
GeneIDi 12932772.
948215.
KEGGi ecj:Y75_p3469.
eco:b3704.
PATRICi 32122905. VBIEscCol129921_3828.

Organism-specific databases

EchoBASEi EB0855.
EcoGenei EG10862. rnpA.

Phylogenomic databases

eggNOGi COG0594.
HOGENOMi HOG000266301.
InParanoidi P0A7Y8.
KOi K03536.
OMAi PPMDFVV.
OrthoDBi EOG6C01C6.
PhylomeDBi P0A7Y8.

Enzyme and pathway databases

BioCyci EcoCyc:EG10862-MONOMER.
ECOL316407:JW3681-MONOMER.
MetaCyc:EG10862-MONOMER.

Miscellaneous databases

PROi P0A7Y8.

Gene expression databases

Genevestigatori P0A7Y8.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
HAMAPi MF_00227. RNase_P.
InterProi IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000100. RNase_P.
IPR020539. RNase_P_CS.
[Graphical view ]
Pfami PF00825. Ribonuclease_P. 1 hit.
[Graphical view ]
ProDomi PD003629. Ribonuclease_P. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF54211. SSF54211. 1 hit.
TIGRFAMsi TIGR00188. rnpA. 1 hit.
PROSITEi PS00648. RIBONUCLEASE_P. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Physical mapping and nucleotide sequence of the rnpA gene that encodes the protein component of ribonuclease P in Escherichia coli."
    Hansen F.G., Hansen E.B., Atlung T.
    Gene 38:85-93(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli."
    Hansen F.G., Hansen E.B., Atlung T.
    EMBO J. 1:1043-1048(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
  6. "Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors."
    Chen W.Y., Singh D., Lai L.B., Stiffler M.A., Lai H.D., Foster M.P., Gopalan V.
    Nucleic Acids Res. 40:4666-4680(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, RNA-BINDING.

Entry informationi

Entry nameiRNPA_ECOLI
AccessioniPrimary (citable) accession number: P0A7Y8
Secondary accession number(s): P06277, Q2M816
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3