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P0A7Y8

- RNPA_ECOLI

UniProt

P0A7Y8 - RNPA_ECOLI

Protein

Ribonuclease P protein component

Gene

rnpA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.1 PublicationUniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.1 PublicationUniRule annotation

    GO - Molecular functioni

    1. ribonuclease P activity Source: EcoCyc
    2. tRNA binding Source: InterPro

    GO - Biological processi

    1. RNA phosphodiester bond hydrolysis Source: GOC
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    3. RNA processing Source: EcoliWiki
    4. tRNA 5'-leader removal Source: EcoliWiki
    5. tRNA processing Source: EcoCyc

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10862-MONOMER.
    ECOL316407:JW3681-MONOMER.
    MetaCyc:EG10862-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein componentUniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P proteinUniRule annotation
    Short name:
    RNaseP proteinUniRule annotation
    Alternative name(s):
    Protein C5UniRule annotation
    Gene namesi
    Name:rnpAUniRule annotation
    Ordered Locus Names:b3704, JW3681
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10862. rnpA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 119119Ribonuclease P protein componentPRO_0000198458Add
    BLAST

    Proteomic databases

    PaxDbiP0A7Y8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A7Y8.

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.1 PublicationUniRule annotation

    Protein-protein interaction databases

    DIPiDIP-36039N.
    IntActiP0A7Y8. 56 interactions.
    MINTiMINT-1218365.
    STRINGi511145.b3704.

    Structurei

    Secondary structure

    1
    119
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 104
    Helixi17 – 237
    Beta strandi33 – 353
    Turni53 – 553
    Helixi59 – 7315
    Turni77 – 793
    Beta strandi82 – 854
    Beta strandi87 – 893
    Helixi93 – 953
    Helixi98 – 11720

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LJPNMR-A1-119[»]
    ProteinModelPortaliP0A7Y8.
    SMRiP0A7Y8. Positions 1-119.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RnpA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0594.
    HOGENOMiHOG000266301.
    KOiK03536.
    OMAiPPMDFVV.
    OrthoDBiEOG6C01C6.
    PhylomeDBiP0A7Y8.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    HAMAPiMF_00227. RNase_P.
    InterProiIPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR000100. RNase_P.
    IPR020539. RNase_P_CS.
    [Graphical view]
    PfamiPF00825. Ribonuclease_P. 1 hit.
    [Graphical view]
    ProDomiPD003629. Ribonuclease_P. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    TIGRFAMsiTIGR00188. rnpA. 1 hit.
    PROSITEiPS00648. RIBONUCLEASE_P. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A7Y8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT    50
    VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA 100
    LSEALEKLWR RHCRLARGS 119
    Length:119
    Mass (Da):13,789
    Last modified:January 1, 1988 - v1
    Checksum:i449D9EDACA1C6240
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11056 Genomic DNA. Translation: AAA24567.1.
    X01861 Genomic DNA. Translation: CAA25983.1.
    L10328 Genomic DNA. Translation: AAA62055.1.
    U00096 Genomic DNA. Translation: AAC76727.1.
    AP009048 Genomic DNA. Translation: BAE77590.1.
    PIRiA00794. NRECP.
    RefSeqiNP_418159.1. NC_000913.3.
    YP_491731.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76727; AAC76727; b3704.
    BAE77590; BAE77590; BAE77590.
    GeneIDi12932772.
    948215.
    KEGGiecj:Y75_p3469.
    eco:b3704.
    PATRICi32122905. VBIEscCol129921_3828.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11056 Genomic DNA. Translation: AAA24567.1 .
    X01861 Genomic DNA. Translation: CAA25983.1 .
    L10328 Genomic DNA. Translation: AAA62055.1 .
    U00096 Genomic DNA. Translation: AAC76727.1 .
    AP009048 Genomic DNA. Translation: BAE77590.1 .
    PIRi A00794. NRECP.
    RefSeqi NP_418159.1. NC_000913.3.
    YP_491731.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LJP NMR - A 1-119 [» ]
    ProteinModelPortali P0A7Y8.
    SMRi P0A7Y8. Positions 1-119.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36039N.
    IntActi P0A7Y8. 56 interactions.
    MINTi MINT-1218365.
    STRINGi 511145.b3704.

    Proteomic databases

    PaxDbi P0A7Y8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76727 ; AAC76727 ; b3704 .
    BAE77590 ; BAE77590 ; BAE77590 .
    GeneIDi 12932772.
    948215.
    KEGGi ecj:Y75_p3469.
    eco:b3704.
    PATRICi 32122905. VBIEscCol129921_3828.

    Organism-specific databases

    EchoBASEi EB0855.
    EcoGenei EG10862. rnpA.

    Phylogenomic databases

    eggNOGi COG0594.
    HOGENOMi HOG000266301.
    KOi K03536.
    OMAi PPMDFVV.
    OrthoDBi EOG6C01C6.
    PhylomeDBi P0A7Y8.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10862-MONOMER.
    ECOL316407:JW3681-MONOMER.
    MetaCyc:EG10862-MONOMER.

    Miscellaneous databases

    PROi P0A7Y8.

    Gene expression databases

    Genevestigatori P0A7Y8.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    HAMAPi MF_00227. RNase_P.
    InterProi IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR000100. RNase_P.
    IPR020539. RNase_P_CS.
    [Graphical view ]
    Pfami PF00825. Ribonuclease_P. 1 hit.
    [Graphical view ]
    ProDomi PD003629. Ribonuclease_P. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    TIGRFAMsi TIGR00188. rnpA. 1 hit.
    PROSITEi PS00648. RIBONUCLEASE_P. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Physical mapping and nucleotide sequence of the rnpA gene that encodes the protein component of ribonuclease P in Escherichia coli."
      Hansen F.G., Hansen E.B., Atlung T.
      Gene 38:85-93(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
      Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
      Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli."
      Hansen F.G., Hansen E.B., Atlung T.
      EMBO J. 1:1043-1048(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
    6. "Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors."
      Chen W.Y., Singh D., Lai L.B., Stiffler M.A., Lai H.D., Foster M.P., Gopalan V.
      Nucleic Acids Res. 40:4666-4680(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, RNA-BINDING.

    Entry informationi

    Entry nameiRNPA_ECOLI
    AccessioniPrimary (citable) accession number: P0A7Y8
    Secondary accession number(s): P06277, Q2M816
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3