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Protein

Ribonuclease HI

Gene

rnhA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium 1By similarity
Metal bindingi10 – 101Magnesium 2By similarity
Metal bindingi48 – 481Magnesium 1By similarity
Metal bindingi70 – 701Magnesium 1By similarity
Metal bindingi134 – 1341Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-215-MONOMER.
ECOO157:RNHA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HI (EC:3.1.26.4)
Short name:
RNase HI
Gene namesi
Name:rnhA
Ordered Locus Names:Z0239, ECs0210
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 155155Ribonuclease HIPRO_0000195373Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

MINTiMINT-1224000.
STRINGi155864.Z0239.

Structurei

3D structure databases

ProteinModelPortaliP0A7Y6.
SMRiP0A7Y6. Positions 1-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 142142RNase HAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.Curated
Contains 1 RNase H domain.Curated

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.
OMAiLVTDSQY.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A7Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM
60 70 80 90 100
AAIVALEALK EHCEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN
110 120 130 140 150
VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERCDELARAA AMNPTLEDTG

YQVEV
Length:155
Mass (Da):17,597
Last modified:July 21, 1986 - v1
Checksum:iB8EF81EEB2F94CBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54510.1.
BA000007 Genomic DNA. Translation: BAB33633.1.
PIRiB85506.
B90655.
RefSeqiNP_308237.1. NC_002695.1.
WP_000917883.1. NZ_LPWC01000417.1.

Genome annotation databases

EnsemblBacteriaiAAG54510; AAG54510; Z0239.
BAB33633; BAB33633; BAB33633.
GeneIDi914053.
KEGGiece:Z0239.
ecs:ECs0210.
PATRICi18349380. VBIEscCol44059_0214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54510.1.
BA000007 Genomic DNA. Translation: BAB33633.1.
PIRiB85506.
B90655.
RefSeqiNP_308237.1. NC_002695.1.
WP_000917883.1. NZ_LPWC01000417.1.

3D structure databases

ProteinModelPortaliP0A7Y6.
SMRiP0A7Y6. Positions 1-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1224000.
STRINGi155864.Z0239.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54510; AAG54510; Z0239.
BAB33633; BAB33633; BAB33633.
GeneIDi914053.
KEGGiece:Z0239.
ecs:ECs0210.
PATRICi18349380. VBIEscCol44059_0214.

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.
OMAiLVTDSQY.
OrthoDBiEOG696BTR.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-215-MONOMER.
ECOO157:RNHA-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiRNH_ECO57
AccessioniPrimary (citable) accession number: P0A7Y6
Secondary accession number(s): P00647, Q8FKY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.