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Protein

Ribonuclease HI

Gene

rnhA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10Magnesium 1By similarity1
Metal bindingi10Magnesium 2By similarity1
Metal bindingi48Magnesium 1By similarity1
Metal bindingi70Magnesium 1By similarity1
Metal bindingi134Magnesium 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HI (EC:3.1.26.4)
Short name:
RNase HI
Gene namesi
Name:rnhA
Ordered Locus Names:c0251
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001953741 – 155Ribonuclease HIAdd BLAST155

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi199310.c0251.

Structurei

3D structure databases

ProteinModelPortaliP0A7Y5.
SMRiP0A7Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 142RNase HAdd BLAST142

Sequence similaritiesi

Belongs to the RNase H family.Curated

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.

Family and domain databases

CDDicd09278. RNase_HI_prokaryote_like. 1 hit.
Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H. 1 hit.
InterProiView protein in InterPro
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR022892. RNaseHI.
PfamiView protein in Pfam
PF00075. RNase_H. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiView protein in PROSITE
PS50879. RNASE_H. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A7Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM
60 70 80 90 100
AAIVALEALK EHCEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN
110 120 130 140 150
VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERCDELARAA AMNPTLEDTG

YQVEV
Length:155
Mass (Da):17,597
Last modified:July 21, 1986 - v1
Checksum:iB8EF81EEB2F94CBE
GO

Sequence cautioni

The sequence AAN78741 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78741.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiAAN78741; AAN78741; c0251.
KEGGiecc:c0251.
PATRICi18278577. VBIEscCol75197_0237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78741.1. Different initiation.

3D structure databases

ProteinModelPortaliP0A7Y5.
SMRiP0A7Y5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN78741; AAN78741; c0251.
KEGGiecc:c0251.
PATRICi18278577. VBIEscCol75197_0237.

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.

Family and domain databases

CDDicd09278. RNase_HI_prokaryote_like. 1 hit.
Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H. 1 hit.
InterProiView protein in InterPro
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR022892. RNaseHI.
PfamiView protein in Pfam
PF00075. RNase_H. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiView protein in PROSITE
PS50879. RNASE_H. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNH_ECOL6
AccessioniPrimary (citable) accession number: P0A7Y5
Secondary accession number(s): P00647, Q8FKY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 15, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.