P0A7Y0 (RNC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	b2567, JW2551

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs. HAMAP MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Enzyme regulation	Activity is down-regulated during cold shock by direct interaction with YmdB. Also down-regulated during entry into stationary phase by an YmdB-independent mechanism. Ref.10
Subunit structure	Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes.
Subcellular location	Cytoplasm HAMAP MF_00104.
Induction	Expression increases as the growth rate increases. Encoded in the rnc-era-recO operon. Ref.9 Ref.10
Disruption phenotype	No visible phenotype. In the absence of Era and Rnc there is a defect in chromosome partitioning. Ref.6 Ref.9
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro rRNA catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Ribonuclease 3 HAMAP MF_00104		PRO_0000180395
Regions
	Domain	6 – 128	123	RNase III
	Domain	155 – 225	71	DRBM
Experimental info
	Mutagenesis	44	1	G → D in rnc-105; loss of activity.
	Sequence conflict	168 – 195	28	HLPLP…HCQVS → PSAAADLSGSPGTWSKRTIR NLLSTARSV in CAA26504. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A7Y0 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: D9E2858F2E0AA3A5	FASTA	226	25,550
	10 20 30 40 50 60 MNPIVINRLQ RKLGYTFNHQ ELLQQALTHR SASSKHNERL EFLGDSILSY VIANALYHRF 70 80 90 100 110 120 PRVDEGDMSR MRATLVRGNT LAELAREFEL GECLRLGPGE LKSGGFRRES ILADTVEALI 130 140 150 160 170 180 GGVFLDSDIQ TVEKLILNWY QTRLDEISPG DKQKDPKTRL QEYLQGRHLP LPTYLVVQVR 190 200 210 220 GEAHDQEFTI HCQVSGLSEP VVGTGSSRRK AEQAAAEQAL KKLELE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of the Escherichia coli ribonuclease III gene and its mutations." Nashimoto H., Uchida H. Mol. Gen. Genet. 201:25-29(1985) [PubMed: 3903434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence of the gene (rnc) encoding ribonuclease III of Escherichia coli." March P.E., Ahnn J., Inouye M. Nucleic Acids Res. 13:4677-4685(1985) [PubMed: 3895158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / CS520.
[3]	Nashimoto H., Saito N. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Genetic analysis of the rnc operon of Escherichia coli." Takiff H.E., Chen S.M., Court D.L. J. Bacteriol. 171:2581-2590(1989) [PubMed: 2540151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45, DISRUPTION PHENOTYPE, OPERON STRUCTURE. Strain: K12 / W3110.
[7]	"Expression and characterization of RNase III and Era proteins. Products of the rnc operon of Escherichia coli." Chen S.M., Takiff H.E., Barber A.M., Dubois G.C., Bardwell J.C., Court D.L. J. Biol. Chem. 265:2888-2895(1990) [PubMed: 2105934] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30, RNA PROCESSING, ATP-BINDING. Strain: K12 / W3110.
[8]	"A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins." Ahnn J., March P.E., Takiff H.E., Inouye M. Proc. Natl. Acad. Sci. U.S.A. 83:8849-8853(1986) [PubMed: 3097637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-226.
[9]	"Cell cycle arrest in Era GTPase mutants: a potential growth rate-regulated checkpoint in Escherichia coli." Britton R.A., Powell B.S., Dasgupta S., Sun Q., Margolin W., Lupski J.R., Court D.L. Mol. Microbiol. 27:739-750(1998) [PubMed: 9515700] [Abstract] Cited for: INDUCTION, DISRUPTION PHENOTYPE. Strain: K12 / W3110.
[10]	"YmdB: a stress-responsive ribonuclease-binding regulator of E. coli RNase III activity." Kim K.S., Manasherob R., Cohen S.N. Genes Dev. 22:3497-3508(2008) [PubMed: 19141481] [Abstract] Cited for: ENZYME REGULATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02946 Genomic DNA. Translation: CAA26692.1. X02673 Genomic DNA. Translation: CAA26504.1. D64044 Genomic DNA. Translation: BAA10914.1. U36841 Genomic DNA. Translation: AAA79829.1. U00096 Genomic DNA. Translation: AAC75620.1. AP009048 Genomic DNA. Translation: BAE76743.1. M26415 Genomic DNA. Translation: AAA21843.1. M14658 Unassigned DNA. Translation: AAA03241.1.
PIR	NREC3. F65034.
RefSeq	NP_417062.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0A7Y0.
SMR	P0A7Y0. Positions 5-225.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48223N.
IntAct	P0A7Y0. 5 interactions.
MINT	MINT-1227120.
PTM databases
PhosSite	P0A7Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001804; EBESCP00000001804; EBESCG00000001489. EBESCT00000001805; EBESCP00000001805; EBESCG00000001489. EBESCT00000015272; EBESCP00000014563; EBESCG00000014332.
GeneID	947033.
GenomeReviews	Gene locus JW2551 in contig AP009048_GR. Gene locus b2567 in contig U00096_GR.
KEGG	ecj:JW2551. eco:b2567.
PATRIC	32120533. VBIEscCol129921_2669.
Organism-specific databases
EchoBASE	EB0850.
EcoGene	EG10857. rnc.
Phylogenomic databases
eggNOG	COG0571.
GeneTree	EBGT00050000011884.
HOGENOM	HBG744556.
OMA	ALTHKSF.
PhylomeDB	P0A7Y0.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10857-MONOMER. MetaCyc:EG10857-MONOMER.
BRENDA	3.1.26.3. 2026.
Gene expression databases
Genevestigator	P0A7Y0.
Family and domain databases
HAMAP	MF_00104. RNase_III. [Tree]
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
KO	K03685.
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_ECOLI

Accession

Primary (citable) accession number: P0A7Y0
Secondary accession number(s): P05797, P06141, Q2MAG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program