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Protein

Ribosome maturation factor RimM

Gene

rimM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late assembly stage of the 30S ribosomal subunit. An accessory protein needed during the final step in assembly of the 30S ribosomal subunit, for assembly of the head region (the 16S rRNA 3' domain) (PubMed:11514519, PubMed:15496525, PubMed:20188109, PubMed:23611982, PubMed:27382067). It may act while Era is associated and before RimP in 30S subunit assembly (PubMed:20188109). Interacts with S19 (PubMed:15496525). Essential for efficient processing of 16S rRNA; a deletion mutant accumulates 17S rRNA (PubMed:9422595). Deletions also do not assemble the head-associated ribosomal proteins correctly (PubMed:23611982, PubMed:27382067). May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes (PubMed:9226267).7 Publications

GO - Molecular functioni

GO - Biological processi

  • maturation of SSU-rRNA Source: EcoliWiki
  • ribosomal small subunit assembly Source: EcoCyc

Keywordsi

Molecular functionChaperone, RNA-binding, rRNA-binding
Biological processRibosome biogenesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11153-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome maturation factor RimM
Alternative name(s):
21K1 Publication
Gene namesi
Name:rimM
Synonyms:yfjA1 Publication
Ordered Locus Names:b2608, JW5413
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11153. rimM.

Subcellular locationi

  • Cytoplasm 2 Publications

  • Note: Binds 30S subunit, but not 70S ribosomes (PubMed:9226267).1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • ribosome Source: InterPro

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Disruption causes slow growth, incomplete processing of 17S rRNA, accumulation of 30S and 50S subunits and decreased translational efficiency (PubMed:9226267, PubMed:23611982). Altered levels of ribosomal proteins in 30S subunits; S2, S3, S14 and S21 severely depleted, S5, S7 and S11 are decreased, distortion of rRNA hleix 44 near the decoding center (PubMed:23611982). Slightly different results show ribosomal proteins S2 and S21 not found in 30S subunits, decreased S3, S13 and S14 in 30S subunits (PubMed:27382067). Deletion is partially suppressed by mutations in rpsM, the gene for ribosomal protein S13 (PubMed:9226267, PubMed:15496525), by a mutation in rpsS, the gene for S19 (PubMed:15496525) and also by increased expression of RbfA (PubMed:9422595). Suppressors due to in-frame fusions with rpsP, the upstream gene for ribosomal protein S16 have been isolated (PubMed:11514519). Suppression is probably due to increased expression of RimM; the fusion proteins are found in translationally active 70S ribosomes (PubMed:11514519).5 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106 – 107YY → AA: 3-fold decreased growth rate; loss of association with 30S ribosomal subunit; loss of interaction with S19. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001632861 – 182Ribosome maturation factor RimMAdd BLAST182

Proteomic databases

EPDiP0A7X6.
PaxDbiP0A7X6.
PRIDEiP0A7X6.

2D gel databases

SWISS-2DPAGEiP0A7X6.

Expressioni

Inductioni

Translation of the mRNA is repressed by a stem-loop in the first 150 nucleotides of its own transcript (PubMed:1569581). Part of the rpsP-rimM-trmD-rplS operon (PubMed:6357787, PubMed:1569581).2 Publications

Interactioni

Subunit structurei

Binds 30S ribosomal subunit but not 70S ribosomes (PubMed:9226267). Binds ribosomal protein S19 and probably 16S rRNA (PubMed:15496525).2 Publications

Protein-protein interaction databases

BioGridi4263071. 57 interactors.
IntActiP0A7X6. 7 interactors.
STRINGi316385.ECDH10B_2775.

Structurei

3D structure databases

ProteinModelPortaliP0A7X6.
SMRiP0A7X6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 182PRC barrelAdd BLAST80

Domaini

The PRC barrel domain binds ribosomal protein S19.1 Publication

Sequence similaritiesi

Belongs to the RimM family.Curated

Phylogenomic databases

eggNOGiENOG4108ZR6. Bacteria.
COG0806. LUCA.
HOGENOMiHOG000220989.
InParanoidiP0A7X6.
KOiK02860.
PhylomeDBiP0A7X6.

Family and domain databases

Gene3Di2.40.30.60. 1 hit.
HAMAPiMF_00014. Ribosome_mat_RimM. 1 hit.
InterProiView protein in InterPro
IPR011961. 16S_RimM.
IPR027275. PRC-brl_dom.
IPR011033. PRC_barrel-like.
IPR002676. RimM.
IPR009000. Transl_B-barrel.
PfamiView protein in Pfam
PF05239. PRC. 1 hit.
PF01782. RimM. 1 hit.
SUPFAMiSSF50346. SSF50346. 1 hit.
SSF50447. SSF50447. 1 hit.
TIGRFAMsiTIGR02273. 16S_RimM. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A7X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQLTAQAP VDPIVLGKMG SSYGIRGWLR VFSSTEDAES IFDYQPWFIQ
60 70 80 90 100
KAGQWQQVQL ESWKHHNQDM IIKLKGVDDR DAANLLTNCE IVVDSSQLPQ
110 120 130 140 150
LEEGDYYWKD LMGCQVVTTE GYDLGKVVDM METGSNDVLV IKANLKDAFG
160 170 180
IKERLVPFLD GQVIKKVDLT TRSIEVDWDP GF
Length:182
Mass (Da):20,605
Last modified:June 7, 2005 - v1
Checksum:iD8512AB9E4FD3D75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01818 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75657.2.
AP009048 Genomic DNA. Translation: BAA16493.2.
PIRiC65039.
RefSeqiNP_417099.4. NC_000913.3.
WP_000043335.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC75657; AAC75657; b2608.
BAA16493; BAA16493; BAA16493.
GeneIDi947101.
KEGGiecj:JW5413.
eco:b2608.
PATRICifig|1411691.4.peg.4131.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRIMM_ECOLI
AccessioniPrimary (citable) accession number: P0A7X6
Secondary accession number(s): P21504, Q8X9D2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 5, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families