30S ribosomal protein S8 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A7W7 (RS8_ECOLI)

Last modified June 16, 2009. Version 44. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
30S ribosomal protein S8

Gene names

Name:	rpsH
Ordered Locus Names:	b3306, JW3268

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit By similarity. Protein S8 is a translational repressor protein, it controls the translation of the spc operon by binding to its mRNA. HAMAP MF_01302
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S5 and S12 By similarity.
Sequence similarities	Belongs to the ribosomal protein S8P family.
Mass spectrometry	Molecular mass is 13993.2 Da from positions 2 - 130. Determined by MALDI. Ref.7

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Ontologies

Keywords
Biological process	Translation regulation
Ligand	RNA-binding rRNA-binding
Molecular function	Repressor Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.4

Chain

2 – 130

129

30S ribosomal protein S8 HAMAP MF_01302

PRO_0000126405

Experimental info

Sequence conflict

E → Q in CAA25719. Ref.1

Secondary structure

130

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A7W7-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 74641BC5021DA651
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FASTA

130

14,127

        10         20         30         40         50         60 
MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK VEGDTKPELE 

        70         80         90        100        110        120 
LTLKYFQGKA VVESIQRVSR PGLRIYKRKD ELPKVMAGLG IAVVSTSKGV MTDRAARQAG 

       130 
LGGEIICYVA

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M. Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The amino acid sequence of the ribosomal protein S8 of Escherichia coli." Allen G., Wittmann-Liebold B. Hoppe-Seyler's Z. Physiol. Chem. 359:1509-1525(1978) [PubMed: 365698] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-130. Strain: K.
[5]	"Translational regulation by ribosomal protein S8 in Escherichia coli: structural homology between rRNA binding site and feedback target on mRNA." Olins P.O., Nomura M. Nucleic Acids Res. 9:1757-1764(1981) [PubMed: 6262737] [Abstract] Cited for: MECHANISM OF TRANSLATION REGULATION.
[6]	"Mutagenesis of ribosomal protein S8 from Escherichia coli: defects in regulation of the spc operon." Wower I., Kowaleski M.P., Sears L.E., Zimmermann R.A. J. Bacteriol. 174:1213-1221(1992) [PubMed: 1735715] [Abstract] Cited for: MUTAGENESIS.
[7]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[9]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[10]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X01563 Genomic DNA. Translation: CAA25719.1.
U18997 Genomic DNA. Translation: AAA58103.1.
U00096 Genomic DNA. Translation: AAC76331.1.
AP009048 Genomic DNA. Translation: BAE77985.1.

PIR

R3EC8. E65123.

RefSeq

AP_004484.1.
NP_417765.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	H	2-130	[»]
1P6G	electron microscopy	12.30	H	2-130	[»]
1P87	electron microscopy	11.50	H	2-130	[»]
1S03	X-ray	2.70	G/H	2-129	[»]
1VS5	X-ray	3.46	H	1-130	[»]
1VS7	X-ray	3.46	H	1-130	[»]
2AVY	X-ray	3.46	H	2-130	[»]
2AW7	X-ray	3.46	H	2-130	[»]
2GY9	electron microscopy	-	H	3-128	[»]
2GYB	electron microscopy	-	H	3-128	[»]
2I2P	X-ray	3.22	H	2-129	[»]
2I2U	X-ray	3.22	H	2-129	[»]
2QAL	X-ray	3.21	H	2-130	[»]
2QAN	X-ray	3.21	H	2-130	[»]
2QB9	X-ray	3.54	H	2-130	[»]
2QBB	X-ray	3.54	H	2-130	[»]
2QBD	X-ray	3.30	H	2-130	[»]
2QBF	X-ray	3.30	H	2-130	[»]
2QBH	X-ray	4.00	H	2-130	[»]
2QBJ	X-ray	4.00	H	2-130	[»]
2QOU	X-ray	3.93	H	2-130	[»]
2QOW	X-ray	3.93	H	2-130	[»]
2QOY	X-ray	3.50	H	2-130	[»]
2QP0	X-ray	3.50	H	2-130	[»]
2VHO	X-ray	3.74	H	2-130	[»]
2VHP	X-ray	3.74	H	2-130	[»]
2Z4K	X-ray	4.45	H	2-130	[»]
2Z4M	X-ray	4.45	H	2-130	[»]
3DF1	X-ray	3.50	H	2-129	[»]
3DF3	X-ray	3.50	H	2-129	[»]
3FIH	electron microscopy	-	H	2-130	[»]

ModBase

Search...

2-D gel databases

ECO2DBASE

I013.5. 6TH EDITION.

Genome annotation databases

GeneID

947802.

GenomeReviews

Gene locus JW3268 in contig AP009048_GR.
Gene locus b3306 in contig U00096_GR.

KEGG

ecj:JW3268.
eco:b3306.

Organism-specific databases

EchoBASE

EB0900.

EcoGene

EG10907. rpsH.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7W7.

OMA

P0A7W7. TRIRNAS.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10907-MON.

Family and domain databases

HAMAP

MF_01302.
[Tree]

InterPro

IPR000630. Ribosomal_S8.
[Graphical view]

PANTHER

PTHR11758. Ribosomal_S8. 1 hit.

Pfam

PF00410. Ribosomal_S8. 1 hit.
[Graphical view]

ProDom

PD001098. Ribosomal_S8. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00053. RIBOSOMAL_S8. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RS8_ECOLI

Accession

Primary (citable) accession number: P0A7W7
Secondary accession number(s): P02361, Q2M6X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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