P0A7W4 (RS5_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S5

Gene names

Name:	rpsE
Ordered Locus Names:	STM3423

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	With S4 and S12 plays an important role in translational accuracy. Many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations). HAMAP MF_01307_B Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body. HAMAP MF_01307_B
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.
Domain	The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity. HAMAP MF_01307_B
Miscellaneous	Altered S5 proteins have been identified in a number of mutants. Some mutations in S5 have been shown to increase translational error frequencies. HAMAP MF_01307_B
Sequence similarities	Belongs to the ribosomal protein S5P family. Contains 1 S5 DRBM domain.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: InterPro
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 167	166	30S ribosomal protein S5 HAMAP MF_01307_B		PRO_0000131589
Regions
	Domain	11 – 74	64	S5 DRBM
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Natural variations
	Natural variant	102	1	G → D Confers streptomycin independence on streptomycin-dependent S12 mutant P90L.
	Natural variant	109	1	G → S in strain: JB279; fast-growing suppressor of streptomycin resistant S12 mutant K42N.
	Natural variant	112	1	R → C Confers streptomycin independence on streptomycin-dependent S12 mutant P90L.
Experimental info
	Sequence conflict	92	1	S → L in CAA11205. Ref.1
	Sequence conflict	95	1	F → I in CAA11205. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A7W4 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0B7EA2CB34018CAB	FASTA	167	17,603
	10 20 30 40 50 60 MAHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI 70 80 90 100 110 120 QKAMEKARRN MINVALNNGT LQHPVKGVHT GSRVFMQPAS EGTGIIAGGA MRAVLEVAGV 130 140 150 160 HNVLAKAYGS TNPINVVRAT IDGLENMNSP EMVAAKRGKS VEEILGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Virulence of antibiotic-resistant Salmonella typhimurium." Bjoerkman J., Hughes D., Andersson D.I. Proc. Natl. Acad. Sci. U.S.A. 95:3949-3953(1998) [PubMed: 9520473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Novel ribosomal mutations affecting translational accuracy, antibiotic resistance and virulence of Salmonella typhimurium." Bjoerkman J., Samuelsson P., Andersson D.I., Hughes D. Mol. Microbiol. 31:53-58(1999) [PubMed: 9987109] [Abstract] Cited for: CHARACTERIZATION OF VARIANTS. Strain: LT2.
[4]	"Limitations of translational accuracy." Kurland C.G., Hughes D., Ehrenberg M. (In) Neidhardt F.C., Curtiss R. III, Ingraham J.L., Lin E.C.C., Low K.B. Magasanik B., Reznikoff W.S., Riley M., Schaechter M., Umbarger H.E. (eds.); Escherichia coli and Salmonella: Cellular and molecular biology (2nd ed.), pp.979-1004, American Society for Microbiology Press, Washington D.C. (1996) Cited for: REVIEW ON TRANSLATIONAL ACCURACY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223237 Genomic DNA. Translation: CAA11205.1. AE006468 Genomic DNA. Translation: AAL22286.1.
RefSeq	NP_462327.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A7W4.
SMR	P0A7W4. Positions 2-167.
ModBase	Search...
Proteomic databases
PRIDE	P0A7W4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1254946.
GenomeReviews	Gene locus STM3423 in contig AE006468_GR.
KEGG	stm:STM3423.
PATRIC	32385645. VBISalEnt20916_3620.
Phylogenomic databases
HOGENOM	HBG299153.
OMA	HNVVKAT.
ProtClustDB	PRK00550.
Enzyme and pathway databases
BioCyc	STYP99287:STM3423-MONOMER.
Family and domain databases
HAMAP	MF_01307_B. Ribosomal_S5_B. [Tree]
InterPro	IPR014720. dsRNA-bd-like. IPR000851. Ribosomal_S5. IPR005712. Ribosomal_S5_bac-type. IPR005324. Ribosomal_S5_C. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR013810. Ribosomal_S5_N. IPR018192. Ribosomal_S5_N_CS. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K02988.
PANTHER	PTHR13718. Ribosomal_S5. 1 hit.
Pfam	PF00333. Ribosomal_S5. 1 hit. PF03719. Ribosomal_S5_C. 1 hit. [Graphical view]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01021. RpsE_bact. 1 hit.
PROSITE	PS00585. RIBOSOMAL_S5. 1 hit. PS50881. S5_DSRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RS5_SALTY

Accession

Primary (citable) accession number: P0A7W4
Secondary accession number(s): O54299, P02356

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program