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Reviewed, UniProtKB/Swiss-Prot P0A7V3 (RS3_ECOLI)

Last modified June 16, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    30S ribosomal protein S3
Gene names
Name: rpsC
Ordered Locus Names: b3314, JW3276
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation By similarity.

Plays a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp. Ref.7

Subunit structure

Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14 By similarity. With proteins S4 and S5 encircles the mRNA as it enters the ribosome, which may play a role in mRNA helicase processivity. Some nascent polypeptide chains are able to cross-link to this protein in situ.

Sequence similarities

Belongs to the ribosomal protein S3P family.

Contains 1 KH type-2 domain.

Mass spectrometry

Molecular mass is 25851.9 Da from positions 2 - 233. Determined by MALDI. Ref.8

Binary interactions

With

Entry

#Exp.

IntAct

Notes

iclRP165281EBI-544851,EBI-552904

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 23323230S ribosomal protein S3 HAMAP MF_01309
PRO_0000130113

Regions

Domain39 – 10769KH type-2

Experimental info

Mutagenesis131 – 1355RRAMK → AAAMA: Decreases mRNA unwinding ability of the ribosome. HAMAP MF_01309

Secondary structure

.................................. 233
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A7V3-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 6D0131A285B7AAB3

FASTA23325,983
        10         20         30         40         50         60 
MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP 

        70         80         90        100        110        120 
AKSIRVTIHT ARPGIVIGKK GEDVEKLRKV VADIAGVPAQ INIAEVRKPE LDAKLVADSI 

       130        140        150        160        170        180 
TSQLERRVMF RRAMKRAVQN AMRLGAKGIK VEVSGRLGGA EIARTEWYRE GRVPLHTLRA 

       190        200        210        220        230 
DIDYNTSEAH TTYGVIGVKV WIFKGEILGG MAAVEQPEKP AAQPKKQQRK GRK 

« Hide

References

« Hide 'large scale' references
[1]"Structure of the Escherichia coli S10 ribosomal protein operon."
Zurawski G., Zurawski S.M.
Nucleic Acids Res. 13:4521-4526(1985) [PubMed: 3892488] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The primary structure of protein S3 from the small ribosomal subunit of Escherichia coli."
Brauer D., Roming R.
FEBS Lett. 106:352-357(1979) [PubMed: 387449] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-233.
Strain: K.
[5]"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract]
Cited for: PROTEIN SEQUENCE OF 39-49 AND 87-108, CROSS-LINKING TO RRNA.
Strain: MRE-600.
[6]"Flexibility of the nascent polypeptide chain within the ribosome --contacts from the peptide N-terminus to a specific region of the 30S subunit."
Choi K.M., Atkins J.F., Gesteland R.F., Brimacombe R.
Eur. J. Biochem. 255:409-413(1998) [PubMed: 9716382] [Abstract]
Cited for: CROSS-LINKING TO NASCENT POLYPEPTIDE CHAINS.
[7]"mRNA helicase activity of the ribosome."
Takyar S., Hickerson R.P., Noller H.F.
Cell 120:49-58(2005) [PubMed: 15652481] [Abstract]
Cited for: ROLE IN MRNA HELICASE ACTIVITY, MUTAGENESIS.
Strain: MRE-600.
[8]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[9]"All-atom homology model of the Escherichia coli 30S ribosomal subunit."
Tung C.-S., Joseph S., Sanbonmatsu K.Y.
Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[10]"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
Cell 113:789-801(2003) [PubMed: 12809609] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
Strain: MRE-600.
[11]"Structures of the bacterial ribosome at 3.5 A resolution."
Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.
Science 310:827-834(2005) [PubMed: 16272117] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
Strain: MRE-600.
+Additional computationally mapped references.

Cross-references

Sequence databases

X02613 Genomic DNA. Translation: CAA26466.1.
U18997 Genomic DNA. Translation: AAA58111.1.
U00096 Genomic DNA. Translation: AAC76339.1.
AP009048 Genomic DNA. Translation: BAE77977.1.
PIRR3EC3. H23129.
RefSeqAP_004476.1.
NP_417773.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1M5Gmodel-C2-207[»]
1P6Gelectron microscopy12.30C2-233[»]
1P87electron microscopy11.50C2-233[»]
1VS5X-ray3.46C1-233[»]
1VS7X-ray3.46C1-233[»]
2AVYX-ray3.46C2-233[»]
2AW7X-ray3.46C2-233[»]
2GY9electron microscopy-C2-206[»]
2GYBelectron microscopy-C2-206[»]
2I2PX-ray3.22C2-232[»]
2I2UX-ray3.22C2-232[»]
2QALX-ray3.21C2-233[»]
2QANX-ray3.21C2-233[»]
2QB9X-ray3.54C2-233[»]
2QBBX-ray3.54C2-233[»]
2QBDX-ray3.30C2-233[»]
2QBFX-ray3.30C2-233[»]
2QBHX-ray4.00C2-233[»]
2QBJX-ray4.00C2-233[»]
2QOUX-ray3.93C2-233[»]
2QOWX-ray3.93C2-233[»]
2QOYX-ray3.50C2-233[»]
2QP0X-ray3.50C2-233[»]
2VHOX-ray3.74C2-233[»]
2VHPX-ray3.74C2-233[»]
2Z4KX-ray4.45C2-233[»]
2Z4MX-ray4.45C2-233[»]
3DF1X-ray3.50C2-232[»]
3DF3X-ray3.50C2-232[»]
3FIHelectron microscopy-C2-207[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP0A7V3. 184 interactions.

Genome annotation databases

GeneID947814.
GenomeReviewsGene locus JW3276 in contig AP009048_GR.
Gene locus b3314 in contig U00096_GR.
KEGGecj:JW3276.
eco:b3314.

Organism-specific databases

EchoBASEEB0895.
EcoGeneEG10902. rpsC.
CMRSearch...

Phylogenomic databases

HOGENOMP0A7V3.
OMAP0A7V3. KPEIDAT.

Enzyme and pathway databases

BioCycEcoCyc:EG10902-MON.

Family and domain databases

HAMAPMF_01309.
[Tree]
InterProIPR004087. KH.
IPR015946. KH-like_a/b.
IPR004044. KH_type_2.
IPR005704. Ribosomal_S3_bac.
IPR001351. Ribosomal_S3_C.
IPR018280. Ribosomal_S3_CS.
IPR008282. Ribosomal_S3_N.
[Graphical view]
Gene3DG3DSA:3.30.300.20. KH_prok. 1 hit.
G3DSA:3.30.1140.32. Ribosomal_S3_C. 1 hit.
PfamPF07650. KH_2. 1 hit.
PF00189. Ribosomal_S3_C. 1 hit.
PF00417. Ribosomal_S3_N. 1 hit.
[Graphical view]
SMARTSM00322. KH. 1 hit.
[Graphical view]
TIGRFAMsTIGR01009. rpsC_bact. 1 hit.
PROSITEPS50823. KH_TYPE_2. 1 hit.
PS00548. RIBOSOMAL_S3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRS3_ECOLI
AccessionPrimary (citable) accession number: P0A7V3
Secondary accession number(s): P02352, Q2M6X9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents