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Reviewed, UniProtKB/Swiss-Prot P0A7V0 (RS2_ECOLI)

Last modified November 3, 2009. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    30S ribosomal protein S2
Gene names
Name: rpsB
Ordered Locus Names: b0169, JW0164
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Subunit structure

Part of the 30S ribosomal subunit. Some nascent polypeptide chains are able to cross-link to this protein in situ. HAMAP MF_00291

Sequence similarities

Belongs to the ribosomal protein S2P family.

Mass spectrometry

Molecular mass is 26610.5 Da from positions 2 - 241. Determined by MALDI. Ref.11

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6 Ref.7 Ref.8
Chain2 – 24124030S ribosomal protein S2 HAMAP MF_00291
PRO_0000134165

Secondary structure

........................................ 241
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A7V0-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 87A38C696115212E

FASTA24126,744
        10         20         30         40         50         60 
MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI 

        70         80         90        100        110        120 
ASRKGKILFV GTKRAASEAV KDAALSCDQF FVNHRWLGGM LTNWKTVRQS IKRLKDLETQ 

       130        140        150        160        170        180 
SQDGTFDKLT KKEALMRTRE LEKLENSLGG IKDMGGLPDA LFVIDADHEH IAIKEANNLG 

       190        200        210        220        230        240 
IPVFAIVDTN SDPDGVDFVI PGNDDAIRAV TLYLGAVAAT VREGRSQDLA SQAEESFVEA 


E 

« Hide

References

« Hide 'large scale' references
[1]"Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts."
An G., Bendiak D.S., Mamelak L.A., Friesen J.D.
Nucleic Acids Res. 9:4163-4172(1981) [PubMed: 6272196] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
Fujita N., Mori H., Yura T., Ishihama A.
Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Primary structure of protein S2 from the Escherichia coli ribosome."
Wittmann-Liebold B., Bosserhoff A.
FEBS Lett. 129:10-16(1981) [PubMed: 7023985] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-241.
Strain: K12.
[7]"Identification of Escherichia coli proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit."
Ueshima R., Fujita N., Ishihama A.
Biochem. Biophys. Res. Commun. 184:634-639(1992) [PubMed: 1575737] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-31.
[8]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-13.
Strain: K12 / EMG2.
[9]"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-35, CROSS-LINKING TO RRNA.
Strain: MRE-600.
[10]"Flexibility of the nascent polypeptide chain within the ribosome --contacts from the peptide N-terminus to a specific region of the 30S subunit."
Choi K.M., Atkins J.F., Gesteland R.F., Brimacombe R.
Eur. J. Biochem. 255:409-413(1998) [PubMed: 9716382] [Abstract]
Cited for: CROSS-LINKING TO NASCENT POLYPEPTIDE CHAINS.
[11]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[12]"All-atom homology model of the Escherichia coli 30S ribosomal subunit."
Tung C.-S., Joseph S., Sanbonmatsu K.Y.
Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[13]"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
Cell 113:789-801(2003) [PubMed: 12809609] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
Strain: MRE-600.
[14]"Structures of the bacterial ribosome at 3.5 A resolution."
Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.
Science 310:827-834(2005) [PubMed: 16272117] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
Strain: MRE-600.
+Additional computationally mapped references.

Cross-references

Sequence databases

V00343 Genomic DNA. Translation: CAA23631.1.
U70214 Genomic DNA. Translation: AAB08598.1.
U00096 Genomic DNA. Translation: AAC73280.1.
AP009048 Genomic DNA. Translation: BAB96744.1.
PIRR3EC2. A02696.
RefSeqAP_000829.1.
NP_414711.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1M5Gmodel-B6-214[»]
1P87electron microscopy11.50B2-241[»]
1VS5X-ray3.46B1-241[»]
1VS7X-ray3.46B1-241[»]
2AVYX-ray3.46B2-241[»]
2AW7X-ray3.46B2-241[»]
2GY9electron microscopy15.00B6-240[»]
2GYBelectron microscopy15.00B6-240[»]
2I2PX-ray3.22B2-240[»]
2I2UX-ray3.22B2-240[»]
2QALX-ray3.21B2-241[»]
2QANX-ray3.21B2-241[»]
2QB9X-ray3.54B2-241[»]
2QBBX-ray3.54B2-241[»]
2QBDX-ray3.30B2-241[»]
2QBFX-ray3.30B2-241[»]
2QBHX-ray4.00B2-241[»]
2QBJX-ray4.00B2-241[»]
2QOUX-ray3.93B2-241[»]
2QOWX-ray3.93B2-241[»]
2QOYX-ray3.50B2-241[»]
2QP0X-ray3.50B2-241[»]
2VHOX-ray3.74B2-241[»]
2VHPX-ray3.74B2-241[»]
2Z4KX-ray4.45B2-241[»]
2Z4MX-ray4.45B2-241[»]
3DF1X-ray3.50B2-240[»]
3DF3X-ray3.50B2-240[»]
3E1Aelectron microscopy-B1-241[»]
3E1Celectron microscopy-B1-241[»]
3FIHelectron microscopy6.70B9-226[»]
3I1MX-ray3.19B1-241[»]
3I1OX-ray3.19B1-241[»]
3I1QX-ray3.81B1-241[»]
3I1SX-ray3.81B1-241[»]
3I1ZX-ray3.71B1-241[»]
3I21X-ray3.71B1-241[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP0A7V0. 204 interactions.
STRINGP0A7V0.

2-D gel databases

SWISS-2DPAGEP0A7V0.
ECO2DBASEH027.0. 6TH EDITION.

Proteomic databases

PRIDEP0A7V0.

Genome annotation databases

GeneID947874.
GenomeReviewsGene locus JW0164 in contig AP009048_GR.
Gene locus b0169 in contig U00096_GR.
KEGGecj:JW0164.
eco:b0169.

Organism-specific databases

EchoBASEEB0894.
EcoGeneEG10901. rpsB.
CMRSearch...

Phylogenomic databases

HOGENOMP0A7V0.
OMALTNFQTV.

Enzyme and pathway databases

BioCycEcoCyc:EG10901-MON.

Gene expression databases

GenevestigatorP0A7V0.

Family and domain databases

HAMAPMF_00291.
[Tree]
InterProIPR001865. Ribosomal_S2.
IPR020589. Ribosomal_S2_bac/chp.
IPR005706. Ribosomal_S2_bac/mit/plastid.
IPR018130. Ribosomal_S2_CS.
[Graphical view]
PANTHERPTHR12534. Ribosom_S2_bac. 1 hit.
PfamPF00318. Ribosomal_S2. 1 hit.
[Graphical view]
PRINTSPR00395. RIBOSOMALS2.
TIGRFAMsTIGR01011. rpsB_bact. 1 hit.
PROSITEPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRS2_ECOLI
AccessionPrimary (citable) accession number: P0A7V0
Secondary accession number(s): P02351, Q9R2E5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents