30S ribosomal protein S20 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A7U7 (RS20_ECOLI)

Last modified June 16, 2009. Version 49. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
30S ribosomal protein S20

Gene names

Name:	rpsT
Ordered Locus Names:	b0023, JW0022

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Binds directly to 16S ribosomal RNA. HAMAP MF_00500
Sequence similarities	Belongs to the ribosomal protein S20P family.
Mass spectrometry	Molecular mass is 9553.6 Da from positions 2 - 87. Determined by MALDI. Ref.8

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Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.7

Chain

2 – 87

30S ribosomal protein S20 HAMAP MF_00500

PRO_0000167958

Experimental info

Sequence conflict

A → R in AAA24604. Ref.2

Secondary structure

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A7U7-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F02154BE2D1E22D9
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FASTA

9,684

        10         20         30         40         50         60 
MANIKSAKKR AIQSEKARKH NASRRSMMRT FIKKVYAAIE AGDKAAAQKA FNEMQPIVDR 

        70         80 
QAAKGLIHKN KAARHKANLT AQINKLA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene for ribosomal protein S20 and its flanking regions." Mackie G.A. J. Biol. Chem. 256:8177-8182(1981) [PubMed: 6267039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon." Kamio Y., Lin C.-K., Regue M., Wu H.C. J. Biol. Chem. 260:5616-5620(1985) [PubMed: 2985604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element." Mackie G.A. Nucleic Acids Res. 14:6965-6981(1986) [PubMed: 2429258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Primary structure of protein S20 from the small ribosomal subunit of Escherichia coli." Wittmann-Liebold B., Marzinzig E., Lehmann A. FEBS Lett. 68:110-114(1976) [PubMed: 786731] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-87. Strain: K.
[8]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[9]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[10]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[11]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M10428 Genomic DNA. Translation: AAA24604.1.
X04382 Genomic DNA. Translation: CAA27968.1.
U00096 Genomic DNA. Translation: AAC73134.1.
AP009048 Genomic DNA. Translation: BAB96593.1.

PIR

R3EC20. A30425.

RefSeq

AP_000687.1.
NP_414564.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	T	3-87	[»]
1P6G	electron microscopy	12.30	T	2-87	[»]
1P87	electron microscopy	11.50	T	2-87	[»]
1VS5	X-ray	3.46	T	1-87	[»]
1VS7	X-ray	3.46	T	1-87	[»]
2AVY	X-ray	3.46	T	2-87	[»]
2AW7	X-ray	3.46	T	2-87	[»]
2GY9	electron microscopy	-	T	5-86	[»]
2GYB	electron microscopy	-	T	5-86	[»]
2I2P	X-ray	3.22	T	2-86	[»]
2I2U	X-ray	3.22	T	2-86	[»]
2QAL	X-ray	3.21	T	2-87	[»]
2QAN	X-ray	3.21	T	2-87	[»]
2QB9	X-ray	3.54	T	2-87	[»]
2QBB	X-ray	3.54	T	2-87	[»]
2QBD	X-ray	3.30	T	2-87	[»]
2QBF	X-ray	3.30	T	2-87	[»]
2QBH	X-ray	4.00	T	2-87	[»]
2QBJ	X-ray	4.00	T	2-87	[»]
2QOU	X-ray	3.93	T	2-87	[»]
2QOW	X-ray	3.93	T	2-87	[»]
2QOY	X-ray	3.50	T	2-87	[»]
2QP0	X-ray	3.50	T	2-87	[»]
2Z4K	X-ray	4.45	T	2-87	[»]
2Z4M	X-ray	4.45	T	2-87	[»]
3DF1	X-ray	3.50	T	2-86	[»]
3DF3	X-ray	3.50	T	2-86	[»]
3FIH	electron microscopy	-	T	3-87	[»]

ModBase

Search...

2-D gel databases

ECO2DBASE

I012.9. 6TH EDITION.

Genome annotation databases

GeneID

944759.

GenomeReviews

Gene locus JW0022 in contig AP009048_GR.
Gene locus b0023 in contig U00096_GR.

KEGG

ecj:JW0022.
eco:b0023.

Organism-specific databases

EchoBASE

EB0912.

EcoGene

EG10919. rpsT.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7U7.

OMA

P0A7U7. SMMRTYI.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10919-MON.

Family and domain databases

HAMAP

MF_00500.
[Tree]

InterPro

IPR002583. Ribosomal_S20.
[Graphical view]

Gene3D

G3DSA:1.20.58.110. Ribosomal_S20. 1 hit.

Pfam

PF01649. Ribosomal_S20p. 1 hit.
[Graphical view]

ProDom

PD004231. Ribosomal_S20p. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00029. S20. 1 hit.

ProtoNet

Search...

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Entry information

Entry name

RS20_ECOLI

Accession

Primary (citable) accession number: P0A7U7
Secondary accession number(s): P02378

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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