30S ribosomal protein S19 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A7U3 (RS19_ECOLI)

Last modified June 16, 2009. Version 50. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
30S ribosomal protein S19

Gene names

Name:	rpsS
Ordered Locus Names:	b3316, JW3278

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	In the E.coli 70S ribosome in the initiation state (Ref.10) it has been modeled to contact the 23S rRNA of the 50S subunit forming part of bridge B1a; this bridge is broken in the model with bound EF-G. The 23S rRNA contact site in bridge B1a is modeled to differ in different ribosomal states (Ref.11), contacting alternately S13 or S19. In the 3.5 angstroms resolved ribosome structures (Ref.12) the contacts between L5, S13 and S19 bridge B1b are different, confirming the dynamic nature of this interaction. Bridge B1a is not visible in the crystallized ribosomes due to 23S rRNA disorder. HAMAP MF_00531 Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. Contacts the A site tRNA. HAMAP MF_00531
Subunit structure	Part of the 30S ribosomal subunit. Binds S13 and L5 and cross-links to the A site tRNA. Ref.10 Ref.12
Sequence similarities	Belongs to the ribosomal protein S19P family.
Mass spectrometry	Molecular mass is 10299.6 Da from positions 2 - 92. Determined by MALDI. Ref.8

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Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.4

Chain

2 – 92

30S ribosomal protein S19 HAMAP MF_00531

PRO_0000129819

Natural variations

Natural variant

H → Y in MW145; suppresses a rimM deletion. HAMAP MF_00531

Experimental info

Sequence conflict

37 – 43

RSTIFPN → STIFPDR AA sequence Ref.4

Sequence conflict

D → N AA sequence Ref.4

Secondary structure

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A7U3-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 439F951848E95CB8
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FASTA

10,430

        10         20         30         40         50         60 
MPRSLKKGPF IDLHLLKKVE KAVESGDKKP LRTWSRRSTI FPNMIGLTIA VHNGRQHVPV 

        70         80         90 
FVTDEMVGHK LGEFAPTRTY RGHAADKKAK KK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the Escherichia coli S10 ribosomal protein operon." Zurawski G., Zurawski S.M. Nucleic Acids Res. 13:4521-4526(1985) [PubMed: 3892488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Primary structure of protein S19 from the small ribosomal subunit of Escherichia coli." Yaguchi M., Wittmann H.G. FEBS Lett. 88:227-230(1978) [PubMed: 348496] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-92. Strain: K.
[5]	"Identification of a cross-link in the Escherichia coli ribosomal protein pair S13-S19 at the amino acid level." Pohl T., Wittmann-Liebold B. J. Biol. Chem. 263:4293-4301(1988) [PubMed: 3279034] [Abstract] Cited for: PROTEIN SEQUENCE OF 66-70, CROSS-LINKING TO S13. Strain: K12 / A19.
[6]	"The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site." Osswald M., Doering T., Brimacombe R. Nucleic Acids Res. 23:4635-4641(1995) [PubMed: 8524654] [Abstract] Cited for: CROSS-LINKING TO THE TRNA CENTRAL FOLD. Strain: MRE-600.
[7]	"The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits." Loevgren J.M., Bylund G.O., Srivastava M.K., Lundberg L.A.C., Persson O.P., Wingsle G., Wikstroem P.M. RNA 10:1798-1812(2004) [PubMed: 15496525] [Abstract] Cited for: ABILITY OF VARIANT MW145 TO SUPPRESS A RIMM DELETION. Strain: MW100.
[8]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[9]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[10]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS), INTERSUBUNIT BRIDGE FORMATION. Strain: MRE-600.
[11]	"Locking and unlocking of ribosomal motions." Valle M., Zavialov A., Sengupta J., Rawat U., Ehrenberg M., Frank J. Cell 114:123-134(2003) [PubMed: 12859903] [Abstract] Cited for: 3D-STRUCTURE MODELING OF RIBOSOMAL COMPLEXES INCLUDING BRIDGE CHANGES UPPON TRANSLOCATION.
[12]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES INCLUDING THE INTERSUBUNIT BRIDGE B1B. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X02613 Genomic DNA. Translation: CAA26464.1.
U18997 Genomic DNA. Translation: AAA58113.1.
U00096 Genomic DNA. Translation: AAC76341.1.
AP009048 Genomic DNA. Translation: BAE77975.1.

PIR

R3EC19. F23129.

RefSeq

AP_004474.1.
NP_417775.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	S	3-81	[»]
1P6G	electron microscopy	12.30	S	2-92	[»]
1P87	electron microscopy	11.50	S	2-92	[»]
1VS5	X-ray	3.46	S	1-92	[»]
1VS7	X-ray	3.46	S	1-92	[»]
2AVY	X-ray	3.46	S	2-92	[»]
2AW7	X-ray	3.46	S	2-92	[»]
2GY9	electron microscopy	-	S	2-87	[»]
2GYB	electron microscopy	-	S	2-87	[»]
2I2P	X-ray	3.22	S	2-91	[»]
2I2U	X-ray	3.22	S	2-91	[»]
2QAL	X-ray	3.21	S	2-92	[»]
2QAN	X-ray	3.21	S	2-92	[»]
2QB9	X-ray	3.54	S	2-92	[»]
2QBB	X-ray	3.54	S	2-92	[»]
2QBD	X-ray	3.30	S	2-92	[»]
2QBF	X-ray	3.30	S	2-92	[»]
2QBH	X-ray	4.00	S	2-92	[»]
2QBJ	X-ray	4.00	S	2-92	[»]
2QOU	X-ray	3.93	S	2-92	[»]
2QOW	X-ray	3.93	S	2-92	[»]
2QOY	X-ray	3.50	S	2-92	[»]
2QP0	X-ray	3.50	S	2-92	[»]
2Z4K	X-ray	4.45	S	2-92	[»]
2Z4M	X-ray	4.45	S	2-92	[»]
3DF1	X-ray	3.50	S	2-91	[»]
3DF3	X-ray	3.50	S	2-91	[»]
3FIH	electron microscopy	-	S	3-81	[»]

ModBase

Search...

Genome annotation databases

GeneID

947811.

GenomeReviews

Gene locus JW3278 in contig AP009048_GR.
Gene locus b3316 in contig U00096_GR.

KEGG

ecj:JW3278.
eco:b3316.

Organism-specific databases

EchoBASE

EB0911.

EcoGene

EG10918. rpsS.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7U3.

OMA

P0A7U3. SGRNEVI.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10918-MON.

Family and domain databases

HAMAP

MF_00531.
[Tree]

InterPro

IPR002222. Ribosomal_S19.
IPR005732. Ribosomal_S19_bac-type.
[Graphical view]

PANTHER

PTHR11880. Ribosomal_S19. 1 hit.

Pfam

PF00203. Ribosomal_S19. 1 hit.
[Graphical view]

PIRSF

PIRSF002144. Ribosomal_S19. 1 hit.

PRINTS

PR00975. RIBOSOMALS19.

ProDom

PD001012. Ribosomal_S19. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01050. rpsS_bact. 1 hit.

PROSITE

PS00323. RIBOSOMAL_S19. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RS19_ECOLI

Accession

Primary (citable) accession number: P0A7U3
Secondary accession number(s): P02375, Q2M6Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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