Reviewed, UniProtKB/Swiss-Prot P0A7T7 (RS18_ECOLI)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S18

Gene names

Name:	rpsR
Ordered Locus Names:	b4202, JW4160

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	75 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit. HAMAP MF_00270
Subunit structure	Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6. HAMAP MF_00270
Sequence similarities	Belongs to the ribosomal protein S18P family.
Mass spectrometry	Molecular mass is 8897.0 Da from positions 2 - 75. Determined by MALDI. Ref.7

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 75

74

30S ribosomal protein S18 HAMAP MF_00270

PRO_0000111153

Amino acid modifications

Modified residue

2

1

N-acetylalanine HAMAP MF_00270

Experimental info

Sequence conflict

24

1

K → E AA sequence Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

75

Helix Strand Turn

Details...

Beta strand

22 – 24

3

Helix

26 – 28

3

Helix

30 – 32

3

Turn

43 – 45

3

Helix

50 – 64

15

Sequences

	Sequence		Length	Mass (Da)
	P0A7T7-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AB2E8288901B73B8	FASTA	75	8,986
	10 20 30 40 50 60 MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK 70 RARYLSLLPY TDRHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of an Escherichia coli chromosomal region containing the genes for ribosomal proteins S6, S18, L9 and an open reading frame." Schnier J., Kitakawa M., Isono K. Mol. Gen. Genet. 204:126-132(1986) [PubMed: 3528756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Primary structure of protein S18 from the small Escherichia coli ribosomal subunit." Yaguchi M. FEBS Lett. 59:217-220(1975) [PubMed: 776663] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-75. Strain: K.
[6]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-38, CROSS-LINKING TO RRNA. Strain: MRE-600.
[7]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[9]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[10]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X04022 Genomic DNA. Translation: CAA27654.1.
U14003 Genomic DNA. Translation: AAA97098.1.
U00096 Genomic DNA. Translation: AAC77159.1.
AP009048 Genomic DNA. Translation: BAE78203.1.

PIR

R3EC18. S56427.

RefSeq

AP_004702.1.
NP_418623.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	R	5-75	[»]
1P6G	electron microscopy	12.30	R	2-75	[»]
1P87	electron microscopy	11.50	R	2-75	[»]
1VS5	X-ray	3.46	R	1-75	[»]
1VS7	X-ray	3.46	R	1-75	[»]
2AVY	X-ray	3.46	R	2-75	[»]
2AW7	X-ray	3.46	R	2-75	[»]
2GY9	electron microscopy	15.00	R	7-74	[»]
2GYB	electron microscopy	15.00	R	7-74	[»]
2I2P	X-ray	3.22	R	2-74	[»]
2I2U	X-ray	3.22	R	2-74	[»]
2QAL	X-ray	3.21	R	2-75	[»]
2QAN	X-ray	3.21	R	2-75	[»]
2QB9	X-ray	3.54	R	2-75	[»]
2QBB	X-ray	3.54	R	2-75	[»]
2QBD	X-ray	3.30	R	2-75	[»]
2QBF	X-ray	3.30	R	2-75	[»]
2QBH	X-ray	4.00	R	2-75	[»]
2QBJ	X-ray	4.00	R	2-75	[»]
2QOU	X-ray	3.93	R	2-75	[»]
2QOW	X-ray	3.93	R	2-75	[»]
2QOY	X-ray	3.50	R	2-75	[»]
2QP0	X-ray	3.50	R	2-75	[»]
2VHO	X-ray	3.74	R	2-75	[»]
2VHP	X-ray	3.74	R	2-75	[»]
2Z4K	X-ray	4.45	R	2-75	[»]
2Z4M	X-ray	4.45	R	2-75	[»]
3DF1	X-ray	3.50	R	2-74	[»]
3DF3	X-ray	3.50	R	2-74	[»]
3E1A	electron microscopy	-	K	1-75	[»]
3E1C	electron microscopy	-	K	1-75	[»]
3FIH	electron microscopy	6.70	R	20-74	[»]
3I1M	X-ray	3.19	R	1-75	[»]
3I1O	X-ray	3.19	R	1-75	[»]
3I1Q	X-ray	3.81	R	1-75	[»]
3I1S	X-ray	3.81	R	1-75	[»]
3I1Z	X-ray	3.71	R	1-75	[»]
3I21	X-ray	3.71	R	1-75	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0A7T7.

Genome annotation databases

GeneID

948721.

GenomeReviews

Gene locus JW4160 in contig AP009048_GR.
Gene locus b4202 in contig U00096_GR.

KEGG

ecj:JW4160.
eco:b4202.

Organism-specific databases

EchoBASE

EB0910.

EcoGene

EG10917. rpsR.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7T7.

OMA

KDKRPKR.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10917-MON.

Gene expression databases

Genevestigator

P0A7T7.

Family and domain databases

HAMAP

MF_00270.
[Tree]

InterPro

IPR001648. Ribosomal_S18.
IPR018275. Ribosomal_S18_CS.
[Graphical view]

Gene3D

G3DSA:4.10.640.10. Ribosomal_S18. 1 hit.

PANTHER

PTHR13479. Ribosomal_S18. 1 hit.

Pfam

PF01084. Ribosomal_S18. 1 hit.
[Graphical view]

PRINTS

PR00974. RIBOSOMALS18.

ProDom

PD002239. Ribosomal_S18. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00165. S18. 1 hit.

PROSITE

PS00057. RIBOSOMAL_S18. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RS18_ECOLI

Accession

Primary (citable) accession number: P0A7T7
Secondary accession number(s): P02374, Q2M6A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)