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Reviewed, UniProtKB/Swiss-Prot P0A7T7 (RS18_ECOLI)

Last modified November 3, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    30S ribosomal protein S18
Gene names
Name: rpsR
Ordered Locus Names: b4202, JW4160
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length75 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit. HAMAP MF_00270

Subunit structure

Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6. HAMAP MF_00270

Sequence similarities

Belongs to the ribosomal protein S18P family.

Mass spectrometry

Molecular mass is 8897.0 Da from positions 2 - 75. Determined by MALDI. Ref.7

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 757430S ribosomal protein S18 HAMAP MF_00270
PRO_0000111153

Amino acid modifications

Modified residue21N-acetylalanine HAMAP MF_00270

Experimental info

Sequence conflict241K → E AA sequence Ref.5

Secondary structure

........... 75
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A7T7-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: AB2E8288901B73B8

FASTA758,986
        10         20         30         40         50         60 
MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK 

        70 
RARYLSLLPY TDRHQ 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of an Escherichia coli chromosomal region containing the genes for ribosomal proteins S6, S18, L9 and an open reading frame."
Schnier J., Kitakawa M., Isono K.
Mol. Gen. Genet. 204:126-132(1986) [PubMed: 3528756] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Primary structure of protein S18 from the small Escherichia coli ribosomal subunit."
Yaguchi M.
FEBS Lett. 59:217-220(1975) [PubMed: 776663] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-75.
Strain: K.
[6]"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract]
Cited for: PROTEIN SEQUENCE OF 31-38, CROSS-LINKING TO RRNA.
Strain: MRE-600.
[7]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]"All-atom homology model of the Escherichia coli 30S ribosomal subunit."
Tung C.-S., Joseph S., Sanbonmatsu K.Y.
Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[9]"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
Cell 113:789-801(2003) [PubMed: 12809609] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
Strain: MRE-600.
[10]"Structures of the bacterial ribosome at 3.5 A resolution."
Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.
Science 310:827-834(2005) [PubMed: 16272117] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
Strain: MRE-600.
+Additional computationally mapped references.

Cross-references

Sequence databases

X04022 Genomic DNA. Translation: CAA27654.1.
U14003 Genomic DNA. Translation: AAA97098.1.
U00096 Genomic DNA. Translation: AAC77159.1.
AP009048 Genomic DNA. Translation: BAE78203.1.
PIRR3EC18. S56427.
RefSeqAP_004702.1.
NP_418623.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1M5Gmodel-R5-75[»]
1P6Gelectron microscopy12.30R2-75[»]
1P87electron microscopy11.50R2-75[»]
1VS5X-ray3.46R1-75[»]
1VS7X-ray3.46R1-75[»]
2AVYX-ray3.46R2-75[»]
2AW7X-ray3.46R2-75[»]
2GY9electron microscopy15.00R7-74[»]
2GYBelectron microscopy15.00R7-74[»]
2I2PX-ray3.22R2-74[»]
2I2UX-ray3.22R2-74[»]
2QALX-ray3.21R2-75[»]
2QANX-ray3.21R2-75[»]
2QB9X-ray3.54R2-75[»]
2QBBX-ray3.54R2-75[»]
2QBDX-ray3.30R2-75[»]
2QBFX-ray3.30R2-75[»]
2QBHX-ray4.00R2-75[»]
2QBJX-ray4.00R2-75[»]
2QOUX-ray3.93R2-75[»]
2QOWX-ray3.93R2-75[»]
2QOYX-ray3.50R2-75[»]
2QP0X-ray3.50R2-75[»]
2VHOX-ray3.74R2-75[»]
2VHPX-ray3.74R2-75[»]
2Z4KX-ray4.45R2-75[»]
2Z4MX-ray4.45R2-75[»]
3DF1X-ray3.50R2-74[»]
3DF3X-ray3.50R2-74[»]
3E1Aelectron microscopy-K1-75[»]
3E1Celectron microscopy-K1-75[»]
3FIHelectron microscopy6.70R20-74[»]
3I1MX-ray3.19R1-75[»]
3I1OX-ray3.19R1-75[»]
3I1QX-ray3.81R1-75[»]
3I1SX-ray3.81R1-75[»]
3I1ZX-ray3.71R1-75[»]
3I21X-ray3.71R1-75[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP0A7T7.

Genome annotation databases

GeneID948721.
GenomeReviewsGene locus JW4160 in contig AP009048_GR.
Gene locus b4202 in contig U00096_GR.
KEGGecj:JW4160.
eco:b4202.

Organism-specific databases

EchoBASEEB0910.
EcoGeneEG10917. rpsR.
CMRSearch...

Phylogenomic databases

HOGENOMP0A7T7.
OMAKDKRPKR.

Enzyme and pathway databases

BioCycEcoCyc:EG10917-MON.

Gene expression databases

GenevestigatorP0A7T7.

Family and domain databases

HAMAPMF_00270.
[Tree]
InterProIPR001648. Ribosomal_S18.
IPR018275. Ribosomal_S18_CS.
[Graphical view]
Gene3DG3DSA:4.10.640.10. Ribosomal_S18. 1 hit.
PANTHERPTHR13479. Ribosomal_S18. 1 hit.
PfamPF01084. Ribosomal_S18. 1 hit.
[Graphical view]
PRINTSPR00974. RIBOSOMALS18.
ProDomPD002239. Ribosomal_S18. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00165. S18. 1 hit.
PROSITEPS00057. RIBOSOMAL_S18. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRS18_ECOLI
AccessionPrimary (citable) accession number: P0A7T7
Secondary accession number(s): P02374, Q2M6A3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents