30S ribosomal protein S16 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A7T3 (RS16_ECOLI)

Last modified June 16, 2009. Version 43. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
30S ribosomal protein S16

Gene names

Name:	rpsP
Ordered Locus Names:	b2609, JW2590

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	82 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function

In addition to being a ribosomal protein, S16 also has a cation-dependent endonuclease activity. Ref.6

In-frame fusions with the ribosome maturation factor rimM suppress mutations in the latter (probably due to increased rimM expression) and are found in translationally active 70S ribosomes. Ref.6

Sequence similarities

Belongs to the ribosomal protein S16P family.

Mass spectrometry

Molecular mass is 9190.5 Da from positions 1 - 82. Determined by MALDI. Ref.9

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Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 82

30S ribosomal protein S16 HAMAP MF_00385

PRO_0000167184

Secondary structure

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A7T3-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: F94D07049A6D489D
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FASTA

9,191

        10         20         30         40         50         60 
MVTIRLARHG AKKRPFYQVV VADSRNARNG RFIERVGFFN PIASEKEEGT RLDLDRIAHW 

        70         80 
VGQGATISDR VAALIKEVNK AA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete amino acid sequence of protein S16 from Escherichia coli." Vandekerckhove J., Rombauts W., Wittmann-Liebold B. Hoppe-Seyler's Z. Physiol. Chem. 358:989-1002(1977) [PubMed: 336510] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: K.
[2]	"The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide." Bystroem A.S., Hjalmarsson K.J., Wikstroem P.M., Bjoerk G.R. EMBO J. 2:899-905(1983) [PubMed: 6357787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The Escherichia coli ribosomal protein S16 is an endonuclease." Oberto J., Bonnefoy E., Mouay E., Pellegrini O., Wilkstroem P.M., Rouviere-Yaniv J. Mol. Microbiol. 19:1319-1330(1996) [PubMed: 8730873] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15, FUNCTION AS AN ENDONUCLEASE.
[7]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[8]	"Hybrid protein between ribosomal protein S16 and RimM of Escherichia coli retains the ribosome maturation function of both proteins." Loevgren J.M., Wikstroem P.M. J. Bacteriol. 183:5352-5357(2001) [PubMed: 11514519] [Abstract] Cited for: FUSION WITH RIBOSOME MATURATION FACTOR RIMM. Strain: MW100.
[9]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[10]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[11]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[12]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X01818 Genomic DNA. Translation: CAA25958.1.
U00096 Genomic DNA. Translation: AAC75658.1.
AP009048 Genomic DNA. Translation: BAA16494.2.

PIR

R3EC16. S07948.

RefSeq

AP_003190.1.
NP_417100.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	P	1-82	[»]
1P6G	electron microscopy	12.30	P	1-82	[»]
1P87	electron microscopy	11.50	P	1-82	[»]
1VS5	X-ray	3.46	P	1-82	[»]
1VS7	X-ray	3.46	P	1-82	[»]
2AVY	X-ray	3.46	P	1-82	[»]
2AW7	X-ray	3.46	P	1-82	[»]
2GY9	electron microscopy	2.00	P	1-78	[»]
2GYB	electron microscopy	2.00	P	1-78	[»]
2I2P	X-ray	3.22	P	1-82	[»]
2I2U	X-ray	3.22	P	1-82	[»]
2QAL	X-ray	3.21	P	1-82	[»]
2QAN	X-ray	3.21	P	1-82	[»]
2QB9	X-ray	3.54	P	1-82	[»]
2QBB	X-ray	3.54	P	1-82	[»]
2QBD	X-ray	3.30	P	1-82	[»]
2QBF	X-ray	3.30	P	1-82	[»]
2QBH	X-ray	4.00	P	1-82	[»]
2QBJ	X-ray	4.00	P	1-82	[»]
2QOU	X-ray	3.93	P	1-82	[»]
2QOW	X-ray	3.93	P	1-82	[»]
2QOY	X-ray	3.50	P	1-82	[»]
2QP0	X-ray	3.50	P	1-82	[»]
2VHO	X-ray	3.74	P	1-82	[»]
2VHP	X-ray	3.74	P	1-82	[»]
2Z4K	X-ray	4.45	P	1-82	[»]
2Z4M	X-ray	4.45	P	1-82	[»]
3DF1	X-ray	3.50	P	1-82	[»]
3DF3	X-ray	3.50	P	1-82	[»]
3FIH	electron microscopy	-	P	1-80	[»]

ModBase

Search...

2-D gel databases

ECO2DBASE

I011.9. 6TH EDITION.

Genome annotation databases

GeneID

947103.

GenomeReviews

Gene locus JW2590 in contig AP009048_GR.
Gene locus b2609 in contig U00096_GR.

KEGG

ecj:JW2590.
eco:b2609.

Organism-specific databases

EchoBASE

EB0908.

EcoGene

EG10915. rpsP.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7T3.

OMA

P0A7T3. YHVVATD.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10915-MON.

Family and domain databases

HAMAP

MF_00385.
[Tree]

InterPro

IPR000307. Ribosomal_S16.
[Graphical view]

Gene3D

G3DSA:3.30.1320.10. Ribosomal_S16. 1 hit.

PANTHER

PTHR12919. Ribosomal_S16. 1 hit.

Pfam

PF00886. Ribosomal_S16. 1 hit.
[Graphical view]

ProDom

PD003791. Ribosomal_S16. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00002. S16. 1 hit.

PROSITE

PS00732. RIBOSOMAL_S16. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RS16_ECOLI

Accession

Primary (citable) accession number: P0A7T3
Secondary accession number(s): P02372, P77006

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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