30S ribosomal protein S10 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A7R5 (RS10_ECOLI)

Last modified June 16, 2009. Version 46. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
30S ribosomal protein S10

Gene names

Name:	rpsJ
Synonyms:	nusE
Ordered Locus Names:	b3321, JW3283

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	103 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in the binding of tRNA to the ribosomes. HAMAP MF_00508
Sequence similarities	Belongs to the ribosomal protein S10P family.
Mass spectrometry	Molecular mass is 11734.5 Da from positions 1 - 103. Determined by MALDI. Ref.8

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Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
rsuA	P0AA43	1	EBI-544602,EBI-557810

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 103

103

30S ribosomal protein S10 HAMAP MF_00508

PRO_0000146529

Secondary structure

103

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A7R5-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: DAEB35A2557F1D59
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FASTA

103

11,736

        10         20         30         40         50         60 
MQNQRIRIRL KAFDHRLIDQ ATAEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD 

        70         80         90        100 
ARDQYEIRTH LRLVDIVEPT EKTVDALMRL DLAAGVDVQI SLG

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of protein S10 from the small ribosomal subunit of Escherichia coli." Yaguchi M., Roy C., Wittmann H.G. FEBS Lett. 121:113-116(1980) [PubMed: 7007073] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Regulation of the S10 ribosomal protein operon in E. coli: nucleotide sequence at the start of the operon." Olins P.O., Nomura M. Cell 26:205-211(1981) [PubMed: 7037196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structure of the Escherichia coli S10 ribosomal protein operon." Zurawski G., Zurawski S.M. Nucleic Acids Res. 13:4521-4526(1985) [PubMed: 3892488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[7]	Noorani S.M., Lindahl L., Zengel J.M. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9. Strain: ECOR 30.
[8]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[9]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[10]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[11]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

V00344 Genomic DNA. Translation: CAA23633.1.
X02613 Genomic DNA. Translation: CAA26459.1.
U18997 Genomic DNA. Translation: AAA58118.1.
U00096 Genomic DNA. Translation: AAC76346.1.
AP009048 Genomic DNA. Translation: BAE77970.1.
AF058450 Genomic DNA. Translation: AAC14290.1.

PIR

R3EC10. A02720.

RefSeq

AP_004469.1.
NP_417780.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	J	5-102	[»]
1P6G	electron microscopy	12.30	J	1-103	[»]
1P87	electron microscopy	11.50	J	1-103	[»]
1VS5	X-ray	3.46	J	1-103	[»]
1VS7	X-ray	3.46	J	1-103	[»]
2AVY	X-ray	3.46	J	1-103	[»]
2AW7	X-ray	3.46	J	1-103	[»]
2GY9	electron microscopy	2.00	J	5-100	[»]
2GYB	electron microscopy	2.00	J	5-100	[»]
2I2P	X-ray	3.22	J	1-103	[»]
2I2U	X-ray	3.22	J	1-103	[»]
2QAL	X-ray	3.21	J	1-103	[»]
2QAN	X-ray	3.21	J	1-103	[»]
2QB9	X-ray	3.54	J	1-103	[»]
2QBB	X-ray	3.54	J	1-103	[»]
2QBD	X-ray	3.30	J	1-103	[»]
2QBF	X-ray	3.30	J	1-103	[»]
2QBH	X-ray	4.00	J	1-103	[»]
2QBJ	X-ray	4.00	J	1-103	[»]
2QOU	X-ray	3.93	J	1-103	[»]
2QOW	X-ray	3.93	J	1-103	[»]
2QOY	X-ray	3.50	J	1-103	[»]
2QP0	X-ray	3.50	J	1-103	[»]
2VHO	X-ray	3.74	J	1-103	[»]
2VHP	X-ray	3.74	J	1-103	[»]
2Z4K	X-ray	4.45	J	1-103	[»]
2Z4M	X-ray	4.45	J	1-103	[»]
3D3B	X-ray	1.30	J	1-103	[»]
3D3C	X-ray	2.60	J/K/L	1-103	[»]
3DF1	X-ray	3.50	J	1-103	[»]
3DF3	X-ray	3.50	J	1-103	[»]
3FIH	electron microscopy	-	J	5-102	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7R5. 96 interactions.

2-D gel databases

ECO2DBASE

I012.1. 6TH EDITION.

Genome annotation databases

GeneID

947816.

GenomeReviews

Gene locus JW3283 in contig AP009048_GR.
Gene locus b3321 in contig U00096_GR.

KEGG

ecj:JW3283.
eco:b3321.

Organism-specific databases

EchoBASE

EB0902.

EcoGene

EG10909. rpsJ.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7R5.

OMA

P0A7R5. STADIVE.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10909-MON.

Family and domain databases

HAMAP

MF_00508.
[Tree]

InterPro

IPR001848. Ribosomal_S10.
IPR005731. Ribosomal_S10_bac.
IPR018268. Ribosomal_S10_CS.
[Graphical view]

Gene3D

G3DSA:3.30.70.600. Ribosomal_S10. 1 hit.

PANTHER

PTHR11700:SF2. Ribos_S10_bac. 1 hit.
PTHR11700. Ribosomal_S10. 1 hit.

Pfam

PF00338. Ribosomal_S10. 1 hit.
[Graphical view]

PRINTS

PR00971. RIBOSOMALS10.

ProDom

PD001272. Ribosomal_S10. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01049. rpsJ_bact. 1 hit.

PROSITE

PS00361. RIBOSOMAL_S10. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00698. Nitrofurantoin.

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Entry information

Entry name

RS10_ECOLI

Accession

Primary (citable) accession number: P0A7R5
Secondary accession number(s): P02364, Q2M6Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2-D gel databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other Resources

Entry information

Relevant documents