P0A7Q6 (RL36_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L36

Alternative name(s):
Ribosomal protein B

Gene names

Name:	rpmJ
Ordered Locus Names:	b3299, JW3261

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	38 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities	Belongs to the ribosomal protein L36P family.
Mass spectrometry	Molecular mass is 4364.2 Da from positions 1 - 38. Determined by MALDI. Ref.5

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 38

38

50S ribosomal protein L36 HAMAP MF_00251

PRO_0000126183

Secondary structure

1

.

.

.

.

.

38

Helix Strand Turn

Details...

Beta strand

11 – 13

3

Beta strand

19 – 21

3

Sequences

	Sequence		Length	Mass (Da)
	P0A7Q6 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: C4785CEC0578EB24	FASTA	38	4,364
	10 20 30 MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of the promoter region for the alpha ribosomal protein operon in Escherichia coli." Post L.E., Arfsten A.E., Davis G.R., Nomura M. J. Biol. Chem. 255:4653-4659(1980) [PubMed: 6154696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Primary structures of and genes for new ribosomal proteins A and B in Escherichia coli." Wada A., Sako T. J. Biochem. 101:817-820(1987) [PubMed: 3298224] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17.
[5]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[6]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[7]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M12432 Genomic DNA. Translation: AAA83902.1.
X01563 Genomic DNA. Translation: CAA25726.1.
U18997 Genomic DNA. Translation: AAA58096.1.
U00096 Genomic DNA. Translation: AAC76324.1.
AP009048 Genomic DNA. Translation: BAE77992.1.

PIR

R5EC36. S14057.

RefSeq

NP_417758.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	4	1-38	[»]
1P86	electron microscopy	11.50	4	1-38	[»]
1VS6	X-ray	3.46	4	1-38	[»]
1VS8	X-ray	3.46	4	1-38	[»]
1VT2	X-ray	3.30	4	1-38	[»]
2AW4	X-ray	3.46	4	1-38	[»]
2AWB	X-ray	3.46	4	1-38	[»]
2I2T	X-ray	3.22	4	1-38	[»]
2I2V	X-ray	3.22	4	1-38	[»]
2J28	electron microscopy	8.00	4	1-38	[»]
2QAM	X-ray	3.21	4	1-38	[»]
2QAO	X-ray	3.21	4	1-38	[»]
2QBA	X-ray	3.54	4	1-38	[»]
2QBC	X-ray	3.54	4	1-38	[»]
2QBE	X-ray	3.30	4	1-38	[»]
2QBG	X-ray	3.30	4	1-38	[»]
2QBI	X-ray	4.00	4	1-38	[»]
2QBK	X-ray	4.00	4	1-38	[»]
2QOV	X-ray	3.93	4	1-38	[»]
2QOX	X-ray	3.93	4	1-38	[»]
2QOZ	X-ray	3.50	4	1-38	[»]
2QP1	X-ray	3.50	4	1-38	[»]
2RDO	electron microscopy	9.10	4	1-38	[»]
2VHM	X-ray	3.74	4	1-38	[»]
2VHN	X-ray	3.74	4	1-38	[»]
2WWQ	electron microscopy	5.80	8	1-38	[»]
2Z4L	X-ray	4.45	4	1-38	[»]
2Z4N	X-ray	4.45	4	1-38	[»]
3BBX	electron microscopy	10.00	4	1-38	[»]
3DF2	X-ray	3.50	4	1-38	[»]
3DF4	X-ray	3.50	4	1-38	[»]
3E1B	electron microscopy	-	X	1-38	[»]
3E1D	electron microscopy	-	X	1-38	[»]
3FIK	electron microscopy	6.70	4	1-38	[»]
3I1N	X-ray	3.19	4	1-38	[»]
3I1P	X-ray	3.19	4	1-38	[»]
3I1R	X-ray	3.81	4	1-38	[»]
3I1T	X-ray	3.81	4	1-38	[»]
3I20	X-ray	3.71	4	1-38	[»]
3I22	X-ray	3.71	4	1-38	[»]
3J01	electron microscopy	-	4	1-38	[»]
3KCR	electron microscopy	-	4	1-38	[»]
3OAS	X-ray	3.25	4	1-38	[»]
3OAT	X-ray	3.25	4	1-38	[»]
3OFC	X-ray	3.19	4	1-38	[»]
3OFD	X-ray	3.19	4	1-38	[»]
3OFQ	X-ray	3.10	4	1-38	[»]
3OFR	X-ray	3.10	4	1-38	[»]
3OFZ	X-ray	3.29	4	1-38	[»]
3OG0	X-ray	3.29	4	1-38	[»]
3ORB	X-ray	3.30	4	1-38	[»]
3R8S	X-ray	3.00	4	1-38	[»]
3R8T	X-ray	3.00	4	1-38	[»]

ProteinModelPortal

P0A7Q6.

SMR

P0A7Q6. Positions 1-38.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7Q6. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002931; EBESCP00000002931; EBESCG00000002396.
EBESCT00000016384; EBESCP00000015675; EBESCG00000015444.

GeneID

947805.

GenomeReviews

Gene locus JW3261 in contig AP009048_GR.
Gene locus b3299 in contig U00096_GR.

KEGG

ecj:JW3261.
eco:b3299.

PATRIC

32122030. VBIEscCol129921_3392.

Organism-specific databases

EchoBASE

EB1214.

EcoGene

EG11232. rpmJ.

Phylogenomic databases

eggNOG

COG0257.

GeneTree

EBGT00050000012119.

HOGENOM

HBG734088.

PhylomeDB

P0A7Q6.

ProtClustDB

PRK00465.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11232-MONOMER.

Gene expression databases

Genevestigator

P0A7Q6.

Family and domain databases

HAMAP

MF_00251. Ribosomal_L36.
[Tree]

InterPro

IPR000473. Ribosomal_L36.
[Graphical view]

KO

K02919.

PANTHER

PTHR18804. Ribosomal_L36. 1 hit.

Pfam

PF00444. Ribosomal_L36. 1 hit.
[Graphical view]

SUPFAM

SSF57840. Ribosomal_L36. 1 hit.

TIGRFAMs

TIGR01022. RpmJ_bact. 1 hit.

PROSITE

PS00828. RIBOSOMAL_L36. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL36_ECOLI

Accession

Primary (citable) accession number: P0A7Q6
Secondary accession number(s): P21194, Q2M6W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program