50S ribosomal protein L35 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A7Q1 (RL35_ECOLI)

Last modified June 16, 2009. Version 40. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
50S ribosomal protein L35
Alternative name(s):
Ribosomal protein A

Gene names

Name:	rpmI
Ordered Locus Names:	b1717, JW1707

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	65 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Sequence similarities	Belongs to the ribosomal protein L35P family.
Mass spectrometry	Molecular mass is 7158.0 Da from positions 2 - 65. Determined by MALDI. Ref.7
Sequence caution	The sequence V00291 differs from that shown. Reason: Frameshift at position 6.

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Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5 Ref.6

Chain

2 – 65

50S ribosomal protein L35 HAMAP MF_00514

PRO_0000177359

Experimental info

Sequence conflict

A → G AA sequence Ref.6

Sequence conflict

A → S Ref.1

Sequence conflict

N → D AA sequence Ref.5

Secondary structure

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A7Q1-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 98A72C0AD6CE0A07
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FASTA

7,289

        10         20         30         40         50         60 
MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA 


CLPYA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon." Sacerdot C., Fayat G., Dessen P., Springer M., Plumbridge J.A., Grunberg-Manago M., Blanquet S. EMBO J. 1:311-315(1982) [PubMed: 6325158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nonspecific inhibition of Escherichia coli ornithine decarboxylase by various ribosomal proteins: detection of a new ribosomal protein possessing strong antizyme activity." Kashiwagi K., Igarashi K. Biochim. Biophys. Acta 911:180-190(1987) [PubMed: 3542048] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-42.
[6]	"Primary structures of and genes for new ribosomal proteins A and B in Escherichia coli." Wada A., Sako T. J. Biochem. 101:817-820(1987) [PubMed: 3298224] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE OF 2-36.
[7]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

V00291 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74787.1.
AP009048 Genomic DNA. Translation: BAA15484.1.

PIR

R5EC35. E64930.

RefSeq

AP_002337.1.
NP_416232.3.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VS6	X-ray	3.46	3	1-65	[»]
1VS8	X-ray	3.46	3	1-65	[»]
2AW4	X-ray	3.46	3	2-65	[»]
2AWB	X-ray	3.46	3	2-65	[»]
2I2T	X-ray	3.22	3	2-64	[»]
2I2V	X-ray	3.22	3	2-64	[»]
2QAM	X-ray	3.21	3	2-65	[»]
2QAO	X-ray	3.21	3	2-65	[»]
2QBA	X-ray	3.54	3	2-65	[»]
2QBC	X-ray	3.54	3	2-65	[»]
2QBE	X-ray	3.30	3	2-65	[»]
2QBG	X-ray	3.30	3	2-65	[»]
2QBI	X-ray	4.00	3	2-65	[»]
2QBK	X-ray	4.00	3	2-65	[»]
2QOV	X-ray	3.93	3	2-65	[»]
2QOX	X-ray	3.93	3	2-65	[»]
2QOZ	X-ray	3.50	3	2-65	[»]
2QP1	X-ray	3.50	3	2-65	[»]
2RDO	electron microscopy	9.10	3	2-65	[»]
2VHM	X-ray	3.74	3	2-65	[»]
2VHN	X-ray	3.74	3	2-65	[»]
2Z4L	X-ray	4.45	3	2-65	[»]
2Z4N	X-ray	4.45	3	2-65	[»]
3BBX	electron microscopy	10.00	3	2-65	[»]
3DF2	X-ray	3.50	3	2-64	[»]
3DF4	X-ray	3.50	3	2-64	[»]
3FIK	electron microscopy	-	3	2-65	[»]

ModBase

Search...

Genome annotation databases

GeneID

946349.

GenomeReviews

Gene locus JW1707 in contig AP009048_GR.
Gene locus b1717 in contig U00096_GR.

KEGG

ecj:JW1707.
eco:b1717.

Organism-specific databases

EchoBASE

EB1213.

EcoGene

EG11231. rpmI.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7Q1.

OMA

P0A7Q1. IKTNRGA.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11231-MON.

Family and domain databases

HAMAP

MF_00514.
[Tree]

InterPro

IPR001706. Ribosomal_L35.
IPR018265. Ribosomal_L35_CS.
[Graphical view]

Pfam

PF01632. Ribosomal_L35p. 1 hit.
[Graphical view]

PRINTS

PR00064. RIBOSOMALL35.

ProDom

PD003417. Ribosomal_L35. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00001. rpmI_bact. 1 hit.

PROSITE

PS00936. RIBOSOMAL_L35. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RL35_ECOLI

Accession

Primary (citable) accession number: P0A7Q1
Secondary accession number(s): P07085, P78275

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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