Reviewed, UniProtKB/Swiss-Prot P0A7N9 (RL33_ECOLI)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L33

Gene names

Name:	rpmG
Ordered Locus Names:	b3636, JW3611

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	55 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Part of the 50S ribosomal subunit. Cross-links to the P and E site tRNAs. HAMAP MF_00294
Miscellaneous	Surface exposed on the 50S subunit. HAMAP MF_00294
Sequence similarities	Belongs to the ribosomal protein L33P family.
Mass spectrometry	Molecular mass is 6254.1 Da from positions 2 - 55. Determined by MALDI. Ref.7

Ontologies

Keywords
Ligand	RNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Methylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 55

54

50S ribosomal protein L33 HAMAP MF_00294

PRO_0000170158

Amino acid modifications

Modified residue

2

1

N-methylalanine HAMAP MF_00294

Experimental info

Sequence conflict

48 – 49

2

IY → YI AA sequence Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

55

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Beta strand

16 – 18

3

Beta strand

28 – 31

4

Beta strand

42 – 44

3

Sequences

	Sequence		Length	Mass (Da)
	P0A7N9-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 31681CC3DAC0C5D6	FASTA	55	6,372
	10 20 30 40 50 MAKGIREKIK LVSSAGTGHF YTTTKNKRTK PEKLELKKFD PVVRQHVIYK EAKIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG)." Lee J.S., An G., Friesen J.D., Isono K. Mol. Gen. Genet. 184:218-223(1981) [PubMed: 7035835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Primary structure of protein L33 from the large subunit of the Escherichia coli ribosome." Wittmann-Liebold B., Pannenbecker R. FEBS Lett. 68:115-118(1976) [PubMed: 786732] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-55. Strain: K.
[6]	"The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site." Osswald M., Doering T., Brimacombe R. Nucleic Acids Res. 23:4635-4641(1995) [PubMed: 8524654] [Abstract] Cited for: CROSS-LINKING TO THE TRNA CENTRAL FOLD. Strain: MRE-600.
[7]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[9]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J01677 Genomic DNA. Translation: AAA74100.1.
L10328 Genomic DNA. Translation: AAA61989.1.
U00096 Genomic DNA. Translation: AAC76660.1.
AP009048 Genomic DNA. Translation: BAE77656.1.

PIR

R5EC33. S42444.

RefSeq

AP_004155.1.
NP_418093.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	1	2-55	[»]
1P86	electron microscopy	11.50	1	2-55	[»]
1VS6	X-ray	3.46	1	1-55	[»]
1VS8	X-ray	3.46	1	1-55	[»]
2AW4	X-ray	3.46	1	2-55	[»]
2AWB	X-ray	3.46	1	2-55	[»]
2GYA	electron microscopy	15.00	1	2-52	[»]
2GYC	electron microscopy	15.00	1	2-53	[»]
2I2T	X-ray	3.22	1	2-54	[»]
2I2V	X-ray	3.22	1	2-54	[»]
2J28	electron microscopy	8.00	1	2-54	[»]
2QAM	X-ray	3.21	1	2-55	[»]
2QAO	X-ray	3.21	1	2-55	[»]
2QBA	X-ray	3.54	1	2-55	[»]
2QBC	X-ray	3.54	1	2-55	[»]
2QBE	X-ray	3.30	1	2-55	[»]
2QBG	X-ray	3.30	1	2-55	[»]
2QBI	X-ray	4.00	1	2-55	[»]
2QBK	X-ray	4.00	1	2-55	[»]
2QOV	X-ray	3.93	1	2-55	[»]
2QOX	X-ray	3.93	1	2-55	[»]
2QOZ	X-ray	3.50	1	2-55	[»]
2QP1	X-ray	3.50	1	2-55	[»]
2RDO	electron microscopy	9.10	1	2-55	[»]
2VHM	X-ray	3.74	1	2-55	[»]
2VHN	X-ray	3.74	1	2-55	[»]
2Z4L	X-ray	4.45	1	2-55	[»]
2Z4N	X-ray	4.45	1	2-55	[»]
3BBX	electron microscopy	10.00	1	2-55	[»]
3DF2	X-ray	3.50	1	2-54	[»]
3DF4	X-ray	3.50	1	2-54	[»]
3E1B	electron microscopy	-	U	2-55	[»]
3E1D	electron microscopy	-	U	2-55	[»]
3FIK	electron microscopy	6.70	1	4-53	[»]
3I1N	X-ray	3.19	1	1-55	[»]
3I1P	X-ray	3.19	1	1-55	[»]
3I1R	X-ray	3.81	1	1-55	[»]
3I1T	X-ray	3.81	1	1-55	[»]
3I20	X-ray	3.71	1	1-55	[»]
3I22	X-ray	3.71	1	1-55	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0A7N9.

Proteomic databases

PRIDE

P0A7N9.

Genome annotation databases

GeneID

946318.

GenomeReviews

Gene locus JW3611 in contig AP009048_GR.
Gene locus b3636 in contig U00096_GR.

KEGG

ecj:JW3611.
eco:b3636.

Organism-specific databases

EchoBASE

EB0884.

EcoGene

EG10891. rpmG.

CMR

Search...

Phylogenomic databases

HOGENOM

HBG751058.

OMA

SKNKRTT.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10891-MON.
ECOL168927:B3636-MON.

Gene expression databases

Genevestigator

P0A7N9.

Family and domain databases

HAMAP

MF_00294.
[Tree]

InterPro

IPR001705. Ribosomal_L33.
IPR018264. Ribosomal_L33_CS.
[Graphical view]

Pfam

PF00471. Ribosomal_L33. 1 hit.
[Graphical view]

PROSITE

PS00582. RIBOSOMAL_L33. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL33_ECOLI

Accession

Primary (citable) accession number: P0A7N9
Secondary accession number(s): P02436, Q2M7V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)