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P0A7N4

- RL32_ECOLI

UniProt

P0A7N4 - RL32_ECOLI

Protein

50S ribosomal protein L32

Gene

rpmF

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10890-MONOMER.
    ECOL316407:JW1075-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L32
    Gene namesi
    Name:rpmF
    Ordered Locus Names:b1089, JW1075
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10890. rpmF.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 575650S ribosomal protein L32PRO_0000172338Add
    BLAST

    Proteomic databases

    PaxDbiP0A7N4.
    PRIDEiP0A7N4.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A7N4.

    Interactioni

    Subunit structurei

    Part of the 50S ribosomal subunit. Contacts L17 (PubMed:2665813).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rpmBP0A7M22EBI-1112732,EBI-543024

    Protein-protein interaction databases

    DIPiDIP-35782N.
    IntActiP0A7N4. 8 interactions.
    STRINGi511145.b1089.

    Structurei

    Secondary structure

    1
    57
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 167
    Helixi17 – 193
    Beta strandi28 – 303
    Beta strandi32 – 343
    Beta strandi37 – 393
    Beta strandi40 – 423
    Beta strandi45 – 495
    Beta strandi50 – 523

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1P85electron microscopy12.30Z2-57[»]
    1P86electron microscopy11.50Z2-57[»]
    1VS6X-ray3.4601-57[»]
    1VS8X-ray3.4601-57[»]
    1VT2X-ray3.3001-57[»]
    2AW4X-ray3.4602-57[»]
    2AWBX-ray3.4602-57[»]
    2GYAelectron microscopy15.00Z28-56[»]
    2GYCelectron microscopy15.00Z28-56[»]
    2I2TX-ray3.2202-57[»]
    2I2VX-ray3.2202-57[»]
    2J28electron microscopy8.0002-57[»]
    2QAMX-ray3.2102-57[»]
    2QAOX-ray3.2102-57[»]
    2QBAX-ray3.5402-57[»]
    2QBCX-ray3.5402-57[»]
    2QBEX-ray3.3002-57[»]
    2QBGX-ray3.3002-57[»]
    2QBIX-ray4.0002-57[»]
    2QBKX-ray4.0002-57[»]
    2QOVX-ray3.9302-57[»]
    2QOXX-ray3.9302-57[»]
    2QOZX-ray3.5002-57[»]
    2QP1X-ray3.5002-57[»]
    2RDOelectron microscopy9.1002-57[»]
    2WWQelectron microscopy5.8032-57[»]
    2Z4LX-ray4.4502-57[»]
    2Z4NX-ray4.4502-57[»]
    3BBXelectron microscopy10.0002-57[»]
    3DF2X-ray3.5002-56[»]
    3DF4X-ray3.5002-56[»]
    3E1Belectron microscopy-T1-57[»]
    3E1Delectron microscopy-T1-57[»]
    3FIKelectron microscopy6.7002-57[»]
    3I1NX-ray3.1901-57[»]
    3I1PX-ray3.1901-57[»]
    3I1RX-ray3.8101-57[»]
    3I1TX-ray3.8101-57[»]
    3I20X-ray3.7101-57[»]
    3I22X-ray3.7101-57[»]
    3IZTelectron microscopy-c1-57[»]
    3IZUelectron microscopy-c1-57[»]
    3J01electron microscopy-02-57[»]
    3J0Telectron microscopy12.1032-57[»]
    3J0Welectron microscopy14.7032-57[»]
    3J0Yelectron microscopy13.5032-57[»]
    3J11electron microscopy13.1032-57[»]
    3J12electron microscopy11.5032-57[»]
    3J14electron microscopy11.5032-57[»]
    3J19electron microscopy8.3002-57[»]
    3J37electron microscopy9.8052-57[»]
    3J4Xelectron microscopy12.0001-57[»]
    3J50electron microscopy20.0001-57[»]
    3J51electron microscopy17.0001-57[»]
    3J52electron microscopy12.0001-57[»]
    3J54electron microscopy13.0001-57[»]
    3J56electron microscopy15.0001-57[»]
    3J58electron microscopy17.0001-57[»]
    3J5Aelectron microscopy12.0001-57[»]
    3J5Celectron microscopy17.0001-57[»]
    3J5Eelectron microscopy17.0001-57[»]
    3J5Gelectron microscopy20.0001-57[»]
    3J5Ielectron microscopy15.0001-57[»]
    3J5Kelectron microscopy9.0001-57[»]
    3J5Lelectron microscopy6.6002-57[»]
    3J5Oelectron microscopy6.8001-57[»]
    3J5Uelectron microscopy7.6032-57[»]
    3J5Welectron microscopy7.6042-57[»]
    3KCRelectron microscopy-01-57[»]
    3OASX-ray3.2502-57[»]
    3OATX-ray3.2502-57[»]
    3OFCX-ray3.1902-57[»]
    3OFDX-ray3.1902-57[»]
    3OFQX-ray3.1002-57[»]
    3OFRX-ray3.1002-57[»]
    3OFZX-ray3.2902-57[»]
    3OG0X-ray3.2902-57[»]
    3ORBX-ray3.3001-57[»]
    3R8SX-ray3.0002-57[»]
    3R8TX-ray3.0002-57[»]
    3SGFX-ray3.2041-57[»]
    3UOSX-ray3.7041-57[»]
    4CSUelectron microscopy5.5032-57[»]
    4GARX-ray3.3001-57[»]
    4GAUX-ray3.3001-57[»]
    4KIXX-ray2.9001-57[»]
    4KIZX-ray2.9001-57[»]
    4KJ1X-ray2.9001-57[»]
    4KJ3X-ray2.9001-57[»]
    4KJ5X-ray2.9001-57[»]
    4KJ7X-ray2.9001-57[»]
    4KJ9X-ray2.9001-57[»]
    4KJBX-ray2.9001-57[»]
    ProteinModelPortaliP0A7N4.
    SMRiP0A7N4. Positions 2-57.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A7N4.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L32P family.Curated

    Phylogenomic databases

    eggNOGiCOG0333.
    HOGENOMiHOG000040269.
    KOiK02911.
    OMAiHRRHHIS.
    OrthoDBiEOG6VMTT4.
    PhylomeDBiP0A7N4.

    Family and domain databases

    HAMAPiMF_00340. Ribosomal_L32.
    InterProiIPR002677. Ribosomal_L32p.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view]
    PfamiPF01783. Ribosomal_L32p. 1 hit.
    [Graphical view]
    SUPFAMiSSF57829. SSF57829. 1 hit.
    TIGRFAMsiTIGR01031. rpmF_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A7N4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR   50
    GRKVIAK 57
    Length:57
    Mass (Da):6,446
    Last modified:January 23, 2007 - v2
    Checksum:iFA07955DAA8E4E94
    GO

    Mass spectrometryi

    Molecular mass is 6315.1 Da from positions 2 - 57. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29698 Genomic DNA. Translation: AAA24575.1.
    U00096 Genomic DNA. Translation: AAC74173.1.
    AP009048 Genomic DNA. Translation: BAA35897.1.
    PIRiJV0048. R5EC32.
    RefSeqiNP_415607.1. NC_000913.3.
    YP_489357.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74173; AAC74173; b1089.
    BAA35897; BAA35897; BAA35897.
    GeneIDi12932035.
    945657.
    KEGGiecj:Y75_p1059.
    eco:b1089.
    PATRICi32117419. VBIEscCol129921_1132.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29698 Genomic DNA. Translation: AAA24575.1 .
    U00096 Genomic DNA. Translation: AAC74173.1 .
    AP009048 Genomic DNA. Translation: BAA35897.1 .
    PIRi JV0048. R5EC32.
    RefSeqi NP_415607.1. NC_000913.3.
    YP_489357.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1P85 electron microscopy 12.30 Z 2-57 [» ]
    1P86 electron microscopy 11.50 Z 2-57 [» ]
    1VS6 X-ray 3.46 0 1-57 [» ]
    1VS8 X-ray 3.46 0 1-57 [» ]
    1VT2 X-ray 3.30 0 1-57 [» ]
    2AW4 X-ray 3.46 0 2-57 [» ]
    2AWB X-ray 3.46 0 2-57 [» ]
    2GYA electron microscopy 15.00 Z 28-56 [» ]
    2GYC electron microscopy 15.00 Z 28-56 [» ]
    2I2T X-ray 3.22 0 2-57 [» ]
    2I2V X-ray 3.22 0 2-57 [» ]
    2J28 electron microscopy 8.00 0 2-57 [» ]
    2QAM X-ray 3.21 0 2-57 [» ]
    2QAO X-ray 3.21 0 2-57 [» ]
    2QBA X-ray 3.54 0 2-57 [» ]
    2QBC X-ray 3.54 0 2-57 [» ]
    2QBE X-ray 3.30 0 2-57 [» ]
    2QBG X-ray 3.30 0 2-57 [» ]
    2QBI X-ray 4.00 0 2-57 [» ]
    2QBK X-ray 4.00 0 2-57 [» ]
    2QOV X-ray 3.93 0 2-57 [» ]
    2QOX X-ray 3.93 0 2-57 [» ]
    2QOZ X-ray 3.50 0 2-57 [» ]
    2QP1 X-ray 3.50 0 2-57 [» ]
    2RDO electron microscopy 9.10 0 2-57 [» ]
    2WWQ electron microscopy 5.80 3 2-57 [» ]
    2Z4L X-ray 4.45 0 2-57 [» ]
    2Z4N X-ray 4.45 0 2-57 [» ]
    3BBX electron microscopy 10.00 0 2-57 [» ]
    3DF2 X-ray 3.50 0 2-56 [» ]
    3DF4 X-ray 3.50 0 2-56 [» ]
    3E1B electron microscopy - T 1-57 [» ]
    3E1D electron microscopy - T 1-57 [» ]
    3FIK electron microscopy 6.70 0 2-57 [» ]
    3I1N X-ray 3.19 0 1-57 [» ]
    3I1P X-ray 3.19 0 1-57 [» ]
    3I1R X-ray 3.81 0 1-57 [» ]
    3I1T X-ray 3.81 0 1-57 [» ]
    3I20 X-ray 3.71 0 1-57 [» ]
    3I22 X-ray 3.71 0 1-57 [» ]
    3IZT electron microscopy - c 1-57 [» ]
    3IZU electron microscopy - c 1-57 [» ]
    3J01 electron microscopy - 0 2-57 [» ]
    3J0T electron microscopy 12.10 3 2-57 [» ]
    3J0W electron microscopy 14.70 3 2-57 [» ]
    3J0Y electron microscopy 13.50 3 2-57 [» ]
    3J11 electron microscopy 13.10 3 2-57 [» ]
    3J12 electron microscopy 11.50 3 2-57 [» ]
    3J14 electron microscopy 11.50 3 2-57 [» ]
    3J19 electron microscopy 8.30 0 2-57 [» ]
    3J37 electron microscopy 9.80 5 2-57 [» ]
    3J4X electron microscopy 12.00 0 1-57 [» ]
    3J50 electron microscopy 20.00 0 1-57 [» ]
    3J51 electron microscopy 17.00 0 1-57 [» ]
    3J52 electron microscopy 12.00 0 1-57 [» ]
    3J54 electron microscopy 13.00 0 1-57 [» ]
    3J56 electron microscopy 15.00 0 1-57 [» ]
    3J58 electron microscopy 17.00 0 1-57 [» ]
    3J5A electron microscopy 12.00 0 1-57 [» ]
    3J5C electron microscopy 17.00 0 1-57 [» ]
    3J5E electron microscopy 17.00 0 1-57 [» ]
    3J5G electron microscopy 20.00 0 1-57 [» ]
    3J5I electron microscopy 15.00 0 1-57 [» ]
    3J5K electron microscopy 9.00 0 1-57 [» ]
    3J5L electron microscopy 6.60 0 2-57 [» ]
    3J5O electron microscopy 6.80 0 1-57 [» ]
    3J5U electron microscopy 7.60 3 2-57 [» ]
    3J5W electron microscopy 7.60 4 2-57 [» ]
    3KCR electron microscopy - 0 1-57 [» ]
    3OAS X-ray 3.25 0 2-57 [» ]
    3OAT X-ray 3.25 0 2-57 [» ]
    3OFC X-ray 3.19 0 2-57 [» ]
    3OFD X-ray 3.19 0 2-57 [» ]
    3OFQ X-ray 3.10 0 2-57 [» ]
    3OFR X-ray 3.10 0 2-57 [» ]
    3OFZ X-ray 3.29 0 2-57 [» ]
    3OG0 X-ray 3.29 0 2-57 [» ]
    3ORB X-ray 3.30 0 1-57 [» ]
    3R8S X-ray 3.00 0 2-57 [» ]
    3R8T X-ray 3.00 0 2-57 [» ]
    3SGF X-ray 3.20 4 1-57 [» ]
    3UOS X-ray 3.70 4 1-57 [» ]
    4CSU electron microscopy 5.50 3 2-57 [» ]
    4GAR X-ray 3.30 0 1-57 [» ]
    4GAU X-ray 3.30 0 1-57 [» ]
    4KIX X-ray 2.90 0 1-57 [» ]
    4KIZ X-ray 2.90 0 1-57 [» ]
    4KJ1 X-ray 2.90 0 1-57 [» ]
    4KJ3 X-ray 2.90 0 1-57 [» ]
    4KJ5 X-ray 2.90 0 1-57 [» ]
    4KJ7 X-ray 2.90 0 1-57 [» ]
    4KJ9 X-ray 2.90 0 1-57 [» ]
    4KJB X-ray 2.90 0 1-57 [» ]
    ProteinModelPortali P0A7N4.
    SMRi P0A7N4. Positions 2-57.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35782N.
    IntActi P0A7N4. 8 interactions.
    STRINGi 511145.b1089.

    Chemistry

    ChEMBLi CHEMBL2363135.

    Proteomic databases

    PaxDbi P0A7N4.
    PRIDEi P0A7N4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74173 ; AAC74173 ; b1089 .
    BAA35897 ; BAA35897 ; BAA35897 .
    GeneIDi 12932035.
    945657.
    KEGGi ecj:Y75_p1059.
    eco:b1089.
    PATRICi 32117419. VBIEscCol129921_1132.

    Organism-specific databases

    EchoBASEi EB0883.
    EcoGenei EG10890. rpmF.

    Phylogenomic databases

    eggNOGi COG0333.
    HOGENOMi HOG000040269.
    KOi K02911.
    OMAi HRRHHIS.
    OrthoDBi EOG6VMTT4.
    PhylomeDBi P0A7N4.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10890-MONOMER.
    ECOL316407:JW1075-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A7N4.
    PROi P0A7N4.

    Gene expression databases

    Genevestigatori P0A7N4.

    Family and domain databases

    HAMAPi MF_00340. Ribosomal_L32.
    InterProi IPR002677. Ribosomal_L32p.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view ]
    Pfami PF01783. Ribosomal_L32p. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57829. SSF57829. 1 hit.
    TIGRFAMsi TIGR01031. rpmF_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of an Escherichia coli operon containing the rpmF gene for ribosomal protein L32 and the gene for a 30-kilodalton protein."
      Tanaka Y., Tsujimura A., Fujita N., Isono S., Isono K.
      J. Bacteriol. 171:5707-5712(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "The primary structure of protein L32 from the 50S subunit of Escherichia coli ribosomes."
      Wittmann-Liebold B., Greuer B., Pannenbecker R.
      Hoppe-Seyler's Z. Physiol. Chem. 356:1977-1979(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-57.
      Strain: K.
    6. "The primary structure of ribosomal protein L32 from E. coli MRE-600 ribosomes."
      Vinokurov L.M., Alakhov Y.B., Golov E.A., Ovchinnikov Y.A.
      Bioorg. Khim. 2:1013-1017(1976)
      Cited for: PROTEIN SEQUENCE OF 2-57.
      Strain: MRE-600.
    7. "Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli."
      Walleczek J., Martin T., Redl B., Stoeffler-Meilicke M., Stoeffler G.
      Biochemistry 28:4099-4105(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: CROSS-LINKING TO L17.
    8. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    9. "Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
      Arnold R.J., Reilly J.P.
      Anal. Biochem. 269:105-112(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
      Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
    10. "Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
      Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
      Cell 113:789-801(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
      Strain: MRE-600.
    11. Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
      Strain: MRE-600.

    Entry informationi

    Entry nameiRL32_ECOLI
    AccessioniPrimary (citable) accession number: P0A7N4
    Secondary accession number(s): P02435
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Ribosomal proteins
      Ribosomal proteins families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3