Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0A7M2 (RL28_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L28
Gene names
Name:rpmB
Ordered Locus Names:b3637, JW3612
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length78 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the ribosomal protein L28P family.

Mass spectrometry

Molecular mass is 8875.0 Da from positions 2 - 78. Determined by MALDI. Ref.8

Binary interactions

With

Entry

#Exp.

IntAct

Notes

rpmFP0A7N42EBI-543024,EBI-1112732

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 787750S ribosomal protein L28 HAMAP-Rule MF_00373
PRO_0000178468

Secondary structure

................... 78
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A7M2 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 2616ADB10772A57C

FASTA789,006
        10         20         30         40         50         60 
MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID 

        70 
KKGIDTVLAE LRARGEKY 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG)."
Lee J.S., An G., Friesen J.D., Isono K.
Mol. Gen. Genet. 184:218-223(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Primary structure of protein L28 from the large subunit of Escherichia coli ribosomes."
Wittmann-Liebold B., Marzinzig E.
FEBS Lett. 81:214-217(1977) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-78.
Strain: K12.
[6]"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 55-66, CROSS-LINKING TO RRNA.
Strain: MRE-600.
[7]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[8]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01677 Genomic DNA. Translation: AAA74099.1.
L10328 Genomic DNA. Translation: AAA61990.1.
U00096 Genomic DNA. Translation: AAC76661.1.
AP009048 Genomic DNA. Translation: BAE77655.1.
PIRR5EC28. S42443.
RefSeqNP_418094.1. NC_000913.3.
YP_491796.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VT2X-ray3.30X1-78[»]
2I2TX-ray3.22X2-77[»]
2I2VX-ray3.22X2-77[»]
2QAMX-ray3.21Z1-78[»]
2QAOX-ray3.21Z1-78[»]
2QBAX-ray3.54Z1-78[»]
2QBCX-ray3.54Z1-78[»]
2QBEX-ray3.30Z1-78[»]
2QBGX-ray3.30Z1-78[»]
2QBIX-ray4.00Z1-78[»]
2QBKX-ray4.00Z1-78[»]
2QOVX-ray3.93Z1-78[»]
2QOXX-ray3.93Z1-78[»]
2QOZX-ray3.50Z1-78[»]
2QP1X-ray3.50Z1-78[»]
2WWQelectron microscopy5.8002-78[»]
2Z4LX-ray4.45Z1-78[»]
2Z4NX-ray4.45Z1-78[»]
3DF2X-ray3.50Z1-78[»]
3DF4X-ray3.50Z1-78[»]
3FIKelectron microscopy6.70X2-78[»]
3I1NX-ray3.19X1-78[»]
3I1PX-ray3.19X1-78[»]
3I1RX-ray3.81X1-78[»]
3I1TX-ray3.81X1-78[»]
3I20X-ray3.71X1-78[»]
3I22X-ray3.71X1-78[»]
3IZTelectron microscopy-Y1-78[»]
3IZUelectron microscopy-Y1-78[»]
3J01electron microscopy-X2-78[»]
3J0Telectron microscopy12.10Z2-77[»]
3J0Welectron microscopy14.70Z2-77[»]
3J0Yelectron microscopy13.50Z2-77[»]
3J11electron microscopy13.10Z2-77[»]
3J12electron microscopy11.50Z2-77[»]
3J14electron microscopy11.50Z2-77[»]
3J19electron microscopy8.30X2-78[»]
3J37electron microscopy9.8012-78[»]
3J4Xelectron microscopy12.00X1-78[»]
3J50electron microscopy20.00X1-78[»]
3J51electron microscopy17.00X1-78[»]
3J52electron microscopy12.00X1-78[»]
3J54electron microscopy13.00X1-78[»]
3J56electron microscopy15.00X1-78[»]
3J58electron microscopy17.00X1-78[»]
3J5Aelectron microscopy12.00X1-78[»]
3J5Celectron microscopy17.00X1-78[»]
3J5Eelectron microscopy17.00X1-78[»]
3J5Gelectron microscopy20.00X1-78[»]
3J5Ielectron microscopy15.00X1-78[»]
3J5Kelectron microscopy9.00X1-78[»]
3J5Oelectron microscopy6.80X1-78[»]
3J5Uelectron microscopy7.60Z2-78[»]
3J5Welectron microscopy7.6012-78[»]
3KCRelectron microscopy-X1-78[»]
3OASX-ray3.25X2-78[»]
3OATX-ray3.25X2-78[»]
3OFCX-ray3.19X2-78[»]
3OFDX-ray3.19X2-78[»]
3OFQX-ray3.10X2-78[»]
3OFRX-ray3.10X2-78[»]
3OFZX-ray3.29X2-78[»]
3OG0X-ray3.29X2-78[»]
3ORBX-ray3.30X1-78[»]
3R8SX-ray3.00X2-78[»]
3R8TX-ray3.00X2-78[»]
3SGFX-ray3.2011-78[»]
3UOSX-ray3.7011-78[»]
4GARX-ray3.30X1-78[»]
4GAUX-ray3.30X1-78[»]
4KIXX-ray2.90X1-78[»]
4KIZX-ray2.90X1-78[»]
4KJ1X-ray2.90X1-78[»]
4KJ3X-ray2.90X1-78[»]
4KJ5X-ray2.90X1-78[»]
4KJ7X-ray2.90X1-78[»]
4KJ9X-ray2.90X1-78[»]
4KJBX-ray2.90X1-78[»]
ProteinModelPortalP0A7M2.
SMRP0A7M2. Positions 2-78.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid851280. 1 interaction.
DIPDIP-35745N.
IntActP0A7M2. 113 interactions.
MINTMINT-1306660.
STRING511145.b3637.

Chemistry

ChEMBLCHEMBL2363135.

PTM databases

PhosSiteP0810433.

Proteomic databases

PaxDbP0A7M2.
PRIDEP0A7M2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76661; AAC76661; b3637.
BAE77655; BAE77655; BAE77655.
GeneID12933571.
946941.
KEGGecj:Y75_p3537.
eco:b3637.
PATRIC32122761. VBIEscCol129921_3757.

Organism-specific databases

EchoBASEEB0879.
EcoGeneEG10886. rpmB.

Phylogenomic databases

eggNOGCOG0227.
HOGENOMHOG000040462.
KOK02902.
OMAFYIPEED.
OrthoDBEOG6W727M.
PhylomeDBP0A7M2.
ProtClustDBPRK00359.

Enzyme and pathway databases

BioCycEcoCyc:EG10886-MONOMER.
ECOL316407:JW3612-MONOMER.

Gene expression databases

GenevestigatorP0A7M2.

Family and domain databases

HAMAPMF_00373. Ribosomal_L28.
InterProIPR001383. Ribosomal_L28.
[Graphical view]
PfamPF00830. Ribosomal_L28. 1 hit.
[Graphical view]
TIGRFAMsTIGR00009. L28. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A7M2.
PROP0A7M2.

Entry information

Entry nameRL28_ECOLI
AccessionPrimary (citable) accession number: P0A7M2
Secondary accession number(s): P02428, Q2M7V1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene