50S ribosomal protein L28 - Escherichia coli (strain K12)

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P0A7M2 (RL28_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 79. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
50S ribosomal protein L28

Gene names

Name:	rpmB
Ordered Locus Names:	b3637, JW3612

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities	Belongs to the ribosomal protein L28P family.
Mass spectrometry	Molecular mass is 8875.0 Da from positions 2 - 78. Determined by MALDI. Ref.8

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rpmF	P0A7N4	1	EBI-543024,EBI-1112732

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5

Chain

2 – 78

50S ribosomal protein L28 HAMAP-Rule MF_00373

PRO_0000178468

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A7M2 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 2616ADB10772A57C
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FASTA

9,006

        10         20         30         40         50         60 
MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID 

        70 
KKGIDTVLAE LRARGEKY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG)." Lee J.S., An G., Friesen J.D., Isono K. Mol. Gen. Genet. 184:218-223(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Primary structure of protein L28 from the large subunit of Escherichia coli ribosomes." Wittmann-Liebold B., Marzinzig E. FEBS Lett. 81:214-217(1977) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-78. Strain: K12.
[6]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 55-66, CROSS-LINKING TO RRNA. Strain: MRE-600.
[7]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
[8]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01677 Genomic DNA. Translation: AAA74099.1.
L10328 Genomic DNA. Translation: AAA61990.1.
U00096 Genomic DNA. Translation: AAC76661.1.
AP009048 Genomic DNA. Translation: BAE77655.1.

PIR

R5EC28. S42443.

RefSeq

NP_418094.1. NC_000913.2.
YP_491796.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VT2	X-ray	3.30	X	1-78	[»]
2I2T	X-ray	3.22	X	2-77	[»]
2I2V	X-ray	3.22	X	2-77	[»]
2QAM	X-ray	3.21	Z	1-78	[»]
2QAO	X-ray	3.21	Z	1-78	[»]
2QBA	X-ray	3.54	Z	1-78	[»]
2QBC	X-ray	3.54	Z	1-78	[»]
2QBE	X-ray	3.30	Z	1-78	[»]
2QBG	X-ray	3.30	Z	1-78	[»]
2QBI	X-ray	4.00	Z	1-78	[»]
2QBK	X-ray	4.00	Z	1-78	[»]
2QOV	X-ray	3.93	Z	1-78	[»]
2QOX	X-ray	3.93	Z	1-78	[»]
2QOZ	X-ray	3.50	Z	1-78	[»]
2QP1	X-ray	3.50	Z	1-78	[»]
2WWQ	electron microscopy	5.80	0	2-78	[»]
2Z4L	X-ray	4.45	Z	1-78	[»]
2Z4N	X-ray	4.45	Z	1-78	[»]
3DF2	X-ray	3.50	Z	1-78	[»]
3DF4	X-ray	3.50	Z	1-78	[»]
3FIK	electron microscopy	6.70	X	2-78	[»]
3I1N	X-ray	3.19	X	1-78	[»]
3I1P	X-ray	3.19	X	1-78	[»]
3I1R	X-ray	3.81	X	1-78	[»]
3I1T	X-ray	3.81	X	1-78	[»]
3I20	X-ray	3.71	X	1-78	[»]
3I22	X-ray	3.71	X	1-78	[»]
3IZT	electron microscopy	-	Y	1-78	[»]
3IZU	electron microscopy	-	Y	1-78	[»]
3J01	electron microscopy	-	X	2-78	[»]
3J0T	electron microscopy	12.10	Z	2-77	[»]
3J0W	electron microscopy	14.70	Z	2-77	[»]
3J0Y	electron microscopy	13.50	Z	2-77	[»]
3J11	electron microscopy	13.10	Z	2-77	[»]
3J12	electron microscopy	11.50	Z	2-77	[»]
3J14	electron microscopy	11.50	Z	2-77	[»]
3J19	electron microscopy	8.30	X	2-78	[»]
3KCR	electron microscopy	-	X	1-78	[»]
3OAS	X-ray	3.25	X	2-78	[»]
3OAT	X-ray	3.25	X	2-78	[»]
3OFC	X-ray	3.19	X	2-78	[»]
3OFD	X-ray	3.19	X	2-78	[»]
3OFQ	X-ray	3.10	X	2-78	[»]
3OFR	X-ray	3.10	X	2-78	[»]
3OFZ	X-ray	3.29	X	2-78	[»]
3OG0	X-ray	3.29	X	2-78	[»]
3ORB	X-ray	3.30	X	1-78	[»]
3R8S	X-ray	3.00	X	2-78	[»]
3R8T	X-ray	3.00	X	2-78	[»]
3SGF	X-ray	3.20	1	1-78	[»]
3UOS	X-ray	3.70	1	1-78	[»]
4GAR	X-ray	3.30	X	1-78	[»]
4GAU	X-ray	3.30	X	1-78	[»]

ProteinModelPortal

P0A7M2.

SMR

P0A7M2. Positions 2-78.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35745N.

IntAct

P0A7M2. 113 interactions.

MINT

MINT-1306660.

STRING

511145.b3637.

PTM databases

PhosSite

P0810433.

Proteomic databases

PaxDb

P0A7M2.

PRIDE

P0A7M2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76661; AAC76661; b3637.
BAE77655; BAE77655; BAE77655.

GeneID

12933571.
946941.

KEGG

ecj:Y75_p3537.
eco:b3637.

PATRIC

32122761. VBIEscCol129921_3757.

Organism-specific databases

EchoBASE

EB0879.

EcoGene

EG10886. rpmB.

Phylogenomic databases

eggNOG

COG0227.

HOGENOM

HOG000040462.

K02902.

OMA

SHARNAT.

ProtClustDB

PRK00359.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10886-MONOMER.
ECOL316407:JW3612-MONOMER.

Gene expression databases

Genevestigator

P0A7M2.

Family and domain databases

HAMAP

MF_00373. Ribosomal_L28.

InterPro

IPR001383. Ribosomal_L28.
[Graphical view]

Pfam

PF00830. Ribosomal_L28. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00009. L28. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A7M2.

Entry information

Entry name

RL28_ECOLI

Accession

Primary (citable) accession number: P0A7M2
Secondary accession number(s): P02428, Q2M7V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Ribosomal proteins

Ribosomal proteins families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents