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Protein

50S ribosomal protein L27

Gene

rpmA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • ribosome binding Source: CAFA
  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: CAFA
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • assembly of large subunit precursor of preribosome Source: CAFA
  • positive regulation of ribosome biogenesis Source: CAFA
  • regulation of cell growth Source: CAFA
  • ribosomal large subunit assembly Source: CAFA
  • translation Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG50002-MONOMER.
MetaCyc:EG50002-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L27
Alternative name(s):
Large ribosomal subunit protein bL271 Publication
Gene namesi
Name:rpmA
Ordered Locus Names:b3185, JW3152
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG50002. rpmA.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: CAFA

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001810852 – 8550S ribosomal protein L27Add BLAST84

Proteomic databases

PaxDbiP0A7L8.
PRIDEiP0A7L8.

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit (PubMed:1225626, PubMed:10094780, PubMed:12809609, PubMed:16272117, PubMed:25310980, PubMed:24844575, PubMed:27934701, PubMed:27906160, PubMed:27906161). Cross-links to the 23S rRNA (PubMed:7556101, PubMed:6170935). Cross-links to the A and P site tRNAs (PubMed:8524654).12 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mqsAQ468642EBI-546875,EBI-1120353

Protein-protein interaction databases

BioGridi4261193. 214 interactors.
849576. 1 interactor.
DIPiDIP-47933N.
IntActiP0A7L8. 26 interactors.
MINTiMINT-1317488.
STRINGi316385.ECDH10B_3359.

Structurei

Secondary structure

185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 25Combined sources4
Beta strandi36 – 39Combined sources4
Beta strandi44 – 47Combined sources4
Beta strandi51 – 53Combined sources3
Beta strandi59 – 72Combined sources14
Turni73 – 76Combined sources4
Beta strandi77 – 83Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J28electron microscopy8.00W2-85[»]
2RDOelectron microscopy9.10W2-85[»]
3BBXelectron microscopy10.00W2-85[»]
3J5Lelectron microscopy6.60W7-85[»]
3J7Zelectron microscopy3.90W1-85[»]
3J8Gelectron microscopy5.00Y1-85[»]
3J9Yelectron microscopy3.90W1-85[»]
3J9Zelectron microscopy3.60LU2-85[»]
3JA1electron microscopy3.60LY2-85[»]
3JBUelectron microscopy3.64y1-85[»]
3JBVelectron microscopy3.32y1-85[»]
3JCDelectron microscopy3.70W1-85[»]
3JCEelectron microscopy3.20W1-85[»]
3JCJelectron microscopy3.70V1-85[»]
3JCNelectron microscopy4.60W1-85[»]
4CSUelectron microscopy5.50Y2-85[»]
4U1UX-ray2.95BW/DW10-85[»]
4U1VX-ray3.00BW/DW10-85[»]
4U20X-ray2.90BW/DW10-85[»]
4U24X-ray2.90BW/DW10-85[»]
4U25X-ray2.90BW/DW10-85[»]
4U26X-ray2.80BW/DW10-85[»]
4U27X-ray2.80BW/DW10-85[»]
4UY8electron microscopy3.80W7-85[»]
4V47electron microscopy12.30AU2-85[»]
4V48electron microscopy11.50AU2-85[»]
4V4HX-ray3.46BW/DW1-85[»]
4V4QX-ray3.46BW/DW2-85[»]
4V4Velectron microscopy15.00BU2-85[»]
4V4Welectron microscopy15.00BU2-85[»]
4V50X-ray3.22BW/DW2-85[»]
4V52X-ray3.21BW/DW2-85[»]
4V53X-ray3.54BW/DW2-85[»]
4V54X-ray3.30BW/DW2-85[»]
4V55X-ray4.00BW/DW2-85[»]
4V56X-ray3.93BW/DW2-85[»]
4V57X-ray3.50BW/DW2-85[»]
4V5BX-ray3.74AW/CW2-85[»]
4V5Helectron microscopy5.80BY7-85[»]
4V5YX-ray4.45BW/DW2-85[»]
4V64X-ray3.50BW/DW2-85[»]
4V65electron microscopy9.00BP1-85[»]
4V66electron microscopy9.00BP1-85[»]
4V69electron microscopy6.70BW7-85[»]
4V6CX-ray3.19BW/DW1-85[»]
4V6DX-ray3.81BW/DW1-85[»]
4V6EX-ray3.71BW/DW1-85[»]
4V6Kelectron microscopy8.25AX1-85[»]
4V6Lelectron microscopy13.20BX1-85[»]
4V6Melectron microscopy7.10BW2-85[»]
4V6Nelectron microscopy12.10AY2-85[»]
4V6Oelectron microscopy14.70BY2-85[»]
4V6Pelectron microscopy13.50BY2-85[»]
4V6Qelectron microscopy11.50BY2-85[»]
4V6Relectron microscopy11.50BY2-85[»]
4V6Selectron microscopy13.10AY2-85[»]
4V6Telectron microscopy8.30BW10-85[»]
4V6Velectron microscopy9.80B02-85[»]
4V6Yelectron microscopy12.00BW7-85[»]
4V6Zelectron microscopy12.00BW7-85[»]
4V70electron microscopy17.00BW7-85[»]
4V71electron microscopy20.00BW7-85[»]
4V72electron microscopy13.00BW7-85[»]
4V73electron microscopy15.00BW7-85[»]
4V74electron microscopy17.00BW7-85[»]
4V75electron microscopy12.00BW7-85[»]
4V76electron microscopy17.00BW7-85[»]
4V77electron microscopy17.00BW7-85[»]
4V78electron microscopy20.00BW7-85[»]
4V79electron microscopy15.00BW7-85[»]
4V7Aelectron microscopy9.00BW7-85[»]
4V7Belectron microscopy6.80BW1-85[»]
4V7Celectron microscopy7.60BY2-85[»]
4V7Delectron microscopy7.60AZ2-85[»]
4V7Ielectron microscopy9.60AW1-85[»]
4V7SX-ray3.25BW/DW7-85[»]
4V7TX-ray3.19BW/DW7-85[»]
4V7UX-ray3.10BW/DW7-85[»]
4V7VX-ray3.29BW/DW7-85[»]
4V85X-ray3.2001-85[»]
4V89X-ray3.70B01-85[»]
4V9CX-ray3.30BW/DW1-85[»]
4V9DX-ray3.00CW10-85[»]
DW11-85[»]
4V9OX-ray2.90AW/CW/EW/GW1-85[»]
4V9PX-ray2.90AW/CW/EW/GW1-85[»]
4WF1X-ray3.09BW/DW10-85[»]
4WOIX-ray3.00BW/CW1-85[»]
4WWWX-ray3.10RW/YW7-85[»]
4YBBX-ray2.10CX/DX10-85[»]
5ADYelectron microscopy4.50W1-85[»]
5AFIelectron microscopy2.90W1-85[»]
5AKAelectron microscopy5.70W2-85[»]
5GADelectron microscopy3.70X1-85[»]
5GAEelectron microscopy3.33X1-85[»]
5GAFelectron microscopy4.30X10-85[»]
5GAGelectron microscopy3.80X1-85[»]
5GAHelectron microscopy3.80X1-85[»]
5H5Uelectron microscopy3.00X2-85[»]
5IQRelectron microscopy3.00W1-85[»]
5IT8X-ray3.12CX/DX10-85[»]
5J5BX-ray2.80CX/DX10-85[»]
5J7LX-ray3.00CX/DX10-85[»]
5J88X-ray3.32CX/DX10-85[»]
5J8AX-ray3.10CX/DX3-85[»]
5J91X-ray2.96CX/DX10-85[»]
5JC9X-ray3.03CX/DX10-85[»]
5JTEelectron microscopy3.60BW1-85[»]
5JU8electron microscopy3.60BW1-85[»]
5KCRelectron microscopy3.60101-85[»]
5KCSelectron microscopy3.90101-85[»]
5KPSelectron microscopy3.90W1-85[»]
5KPVelectron microscopy4.10V1-85[»]
5KPWelectron microscopy3.90V1-85[»]
5KPXelectron microscopy3.90V1-85[»]
5L3Pelectron microscopy3.7001-85[»]
5LZAelectron microscopy3.60W11-85[»]
5LZBelectron microscopy5.30W11-85[»]
5LZCelectron microscopy4.80W11-85[»]
5LZDelectron microscopy3.40W11-85[»]
5LZEelectron microscopy3.50W11-85[»]
5LZFelectron microscopy4.60W11-85[»]
5MDVelectron microscopy2.97W1-85[»]
5MDWelectron microscopy3.06W1-85[»]
5MDYelectron microscopy3.35W1-85[»]
5MDZelectron microscopy3.10W1-85[»]
5MGPelectron microscopy3.10W11-85[»]
5NCOelectron microscopy4.80X10-85[»]
5NP6electron microscopy3.60u11-85[»]
5U4Ielectron microscopy3.50X1-85[»]
5U9Felectron microscopy3.20251-85[»]
5U9Gelectron microscopy3.20251-85[»]
5UYKelectron microscopy3.902511-85[»]
5UYLelectron microscopy3.602511-85[»]
5UYMelectron microscopy3.202511-85[»]
5UYNelectron microscopy4.002511-85[»]
5UYPelectron microscopy3.902511-85[»]
5UYQelectron microscopy3.802511-85[»]
DisProtiDP00140.
ProteinModelPortaliP0A7L8.
SMRiP0A7L8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7L8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105K46. Bacteria.
COG0211. LUCA.
HOGENOMiHOG000111610.
InParanoidiP0A7L8.
KOiK02899.
PhylomeDBiP0A7L8.

Family and domain databases

HAMAPiMF_00539. Ribosomal_L27. 1 hit.
InterProiView protein in InterPro
IPR001684. Ribosomal_L27.
IPR018261. Ribosomal_L27_CS.
PANTHERiPTHR15893. PTHR15893. 1 hit.
PfamiView protein in Pfam
PF01016. Ribosomal_L27. 1 hit.
PRINTSiPR00063. RIBOSOMALL27.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD003114. Ribosomal_L27. 1 hit.
TIGRFAMsiTIGR00062. L27. 1 hit.
PROSITEiView protein in PROSITE
PS00831. RIBOSOMAL_L27. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7L8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN
60 70 80
VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE
Length:85
Mass (Da):9,124
Last modified:January 23, 2007 - v2
Checksum:iB43AAE180ABC23EA
GO

Mass spectrometryi

Molecular mass is 8993.5 Da from positions 2 - 85. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13267 Genomic DNA. Translation: BAA02526.1.
U18997 Genomic DNA. Translation: AAA57986.1.
U00096 Genomic DNA. Translation: AAC76217.1.
AP009048 Genomic DNA. Translation: BAE77229.1.
PIRiJS0767. R5EC27.
RefSeqiNP_417652.1. NC_000913.3.
WP_000940595.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76217; AAC76217; b3185.
BAE77229; BAE77229; BAE77229.
GeneIDi945190.
KEGGiecj:JW3152.
eco:b3185.
PATRICifig|1411691.4.peg.3546.

Similar proteinsi

Entry informationi

Entry nameiRL27_ECOLI
AccessioniPrimary (citable) accession number: P0A7L8
Secondary accession number(s): P02427, Q2M927
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families