50S ribosomal protein L20 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A7L3 (RL20_ECOLI)

Last modified June 16, 2009. Version 48. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
50S ribosomal protein L20

Gene names

Name:	rplT
Synonyms:	pdzA
Ordered Locus Names:	b1716, JW1706

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds close to the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. HAMAP MF_00382
Subunit structure	Part of the 50S ribosomal subunit. Contacts L21 (Ref.9). Ref.8
Sequence similarities	Belongs to the ribosomal protein L20P family.
Mass spectrometry	Molecular mass is 13366.9 Da from positions 2 - 118. Determined by MALDI. Ref.10

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Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ribosomal large subunit assembly Inferred from electronic annotation. Source: HAMAP translation Inferred from electronic annotation. Source: InterPro
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.6

Chain

2 – 118

117

50S ribosomal protein L20 HAMAP MF_00382

PRO_0000177156

Experimental info

Sequence conflict

R → A in CAA23562. Ref.1

Sequence conflict

R → A in AAA51468. Ref.1

Secondary structure

118

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A7L3-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: E60AFDBB6F05DFB3
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FASTA

118

13,497

        10         20         30         40         50         60 
MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL 

        70         80         90        100        110 
WIARINAAAR QNGISYSKFI NGLKKASVEI DRKILADIAV FDKVAFTALV EKAKAALA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20." Fayat G., Mayaux J.-F., Sacerdot C., Fromant M., Springer M., Grunberg-Manago M., Blanquet S. J. Mol. Biol. 171:239-261(1983) [PubMed: 6317865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Primary structure of the himA gene of Escherichia coli: homology with DNA-binding protein HU and association with the phenylalanyl-tRNA synthetase operon." Miller H.I. Cold Spring Harb. Symp. Quant. Biol. 49:691-698(1984) [PubMed: 6397321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The primary structure of protein L20 from the large subunit of the Escherichia coli ribosome." Wittmann-Liebold B., Seib C. FEBS Lett. 103:61-65(1979) [PubMed: 381019] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-118. Strain: K12.
[7]	"Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon." Springer M., Mayaux J.-F., Fayat G., Plumbridge J.A., Graffe M., Blanquet S., Grunberg-Manago M. J. Mol. Biol. 181:467-478(1985) [PubMed: 3158742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 110-118.
[8]	"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes." Herold M., Nierhaus K.H. J. Biol. Chem. 262:8826-8833(1987) [PubMed: 3298242] [Abstract] Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT. Strain: K12.
[9]	"Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli." Walleczek J., Martin T., Redl B., Stoeffler-Meilicke M., Stoeffler G. Biochemistry 28:4099-4105(1989) [PubMed: 2665813] [Abstract] Cited for: CROSS-LINKING TO L21.
[10]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[11]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[12]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

V00291 Genomic DNA. Translation: CAA23562.1.
K02844 Genomic DNA. Translation: AAA51468.1.
U00096 Genomic DNA. Translation: AAC74786.1.
AP009048 Genomic DNA. Translation: BAA15483.1.
M10423 Genomic DNA. Translation: AAA23960.1.

PIR

R5EC20. D64930.

RefSeq

AP_002336.1.
NP_416231.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	O	2-118	[»]
1P86	electron microscopy	11.50	O	2-118	[»]
1VS6	X-ray	3.46	Q	1-118	[»]
1VS8	X-ray	3.46	Q	1-118	[»]
2AW4	X-ray	3.46	Q	2-118	[»]
2AWB	X-ray	3.46	Q	2-118	[»]
2GYA	electron microscopy	-	O	3-116	[»]
2GYC	electron microscopy	2.00	O	3-117	[»]
2I2T	X-ray	3.22	Q	2-117	[»]
2I2V	X-ray	3.22	Q	2-117	[»]
2J28	electron microscopy	8.00	Q	2-117	[»]
2QAM	X-ray	3.21	Q	2-118	[»]
2QAO	X-ray	3.21	Q	2-118	[»]
2QBA	X-ray	3.54	Q	2-118	[»]
2QBC	X-ray	3.54	Q	2-118	[»]
2QBE	X-ray	3.30	Q	2-118	[»]
2QBG	X-ray	3.30	Q	2-118	[»]
2QBI	X-ray	4.00	Q	2-118	[»]
2QBK	X-ray	4.00	Q	2-118	[»]
2QOV	X-ray	3.93	Q	2-118	[»]
2QOX	X-ray	3.93	Q	2-118	[»]
2QOZ	X-ray	3.50	Q	2-118	[»]
2QP1	X-ray	3.50	Q	2-118	[»]
2RDO	electron microscopy	9.10	Q	2-118	[»]
2VHM	X-ray	3.74	Q	2-118	[»]
2VHN	X-ray	3.74	Q	2-118	[»]
2Z4L	X-ray	4.45	Q	2-118	[»]
2Z4N	X-ray	4.45	Q	2-118	[»]
3BBX	electron microscopy	10.00	Q	2-118	[»]
3DF2	X-ray	3.50	Q	2-117	[»]
3DF4	X-ray	3.50	Q	2-117	[»]
3FIK	electron microscopy	-	Q	2-118	[»]

ModBase

Search...

Genome annotation databases

GeneID

945152.

GenomeReviews

Gene locus JW1706 in contig AP009048_GR.
Gene locus b1716 in contig U00096_GR.

KEGG

ecj:JW1706.
eco:b1716.

Organism-specific databases

EchoBASE

EB0874.

EcoGene

EG10881. rplT.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7L3.

OMA

P0A7L3. FYGRRKN.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10881-MON.

Family and domain databases

HAMAP

MF_00382.
[Tree]

InterPro

IPR005813. Ribosomal_L20.
IPR005812. Ribosomal_L20_bac-type.
[Graphical view]

PANTHER

PTHR10986. Ribosomal_L20. 1 hit.

Pfam

PF00453. Ribosomal_L20. 1 hit.
[Graphical view]

PRINTS

PR00062. RIBOSOMALL20.

ProDom

PD002389. Ribosomal_L20. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01032. rplT_bact. 1 hit.

PROSITE

PS00937. RIBOSOMAL_L20. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RL20_ECOLI

Accession

Primary (citable) accession number: P0A7L3
Secondary accession number(s): P02421, Q47253

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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