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Protein

50S ribosomal protein L20

Gene

rplT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins, it binds close to the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly.1 Publication

GO - Molecular functioni

  • large ribosomal subunit rRNA binding Source: EcoCyc
  • mRNA binding Source: EcoCyc
  • structural constituent of ribosome Source: EcoCyc
  • translation repressor activity, mRNA regulatory element binding Source: EcoCyc

GO - Biological processi

  • ribosomal large subunit assembly Source: EcoCyc
  • translation Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10881-MONOMER
MetaCyc:EG10881-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L20
Alternative name(s):
Large ribosomal subunit protein bL201 Publication
Gene namesi
Name:rplT
Synonyms:pdzA
Ordered Locus Names:b1716, JW1706
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10881 rplT

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • cytosolic large ribosomal subunit Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001771562 – 11850S ribosomal protein L20Add BLAST117

Proteomic databases

EPDiP0A7L3
PaxDbiP0A7L3
PRIDEiP0A7L3

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit (PubMed:381019, PubMed:10094780, PubMed:12809609, PubMed:16272117, PubMed:25310980, PubMed:24844575, PubMed:27934701, PubMed:27906160, PubMed:27906161). Contacts L21 (PubMed:2665813).10 Publications

Protein-protein interaction databases

DIPiDIP-47941N
IntActiP0A7L3, 39 interactors
STRINGi316385.ECDH10B_1852

Structurei

Secondary structure

1118
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 20Combined sources14
Turni21 – 23Combined sources3
Helixi26 – 29Combined sources4
Helixi32 – 71Combined sources40
Helixi76 – 85Combined sources10
Helixi92 – 101Combined sources10
Helixi103 – 117Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J28electron microscopy8.00Q2-118[»]
2RDOelectron microscopy9.10Q2-118[»]
3BBXelectron microscopy10.00Q2-118[»]
3J5Lelectron microscopy6.60Q2-118[»]
3J7Zelectron microscopy3.90Q1-118[»]
3J8Gelectron microscopy5.00Q1-118[»]
3J9Yelectron microscopy3.90Q1-118[»]
3J9Zelectron microscopy3.60LO2-118[»]
3JA1electron microscopy3.60LS2-118[»]
3JBUelectron microscopy3.64q1-118[»]
3JBVelectron microscopy3.32q1-118[»]
3JCDelectron microscopy3.70Q1-118[»]
3JCEelectron microscopy3.20Q1-118[»]
3JCJelectron microscopy3.70P1-118[»]
3JCNelectron microscopy4.60Q1-118[»]
4CSUelectron microscopy5.50Q2-118[»]
4U1UX-ray2.95BQ/DQ2-118[»]
4U1VX-ray3.00BQ/DQ2-118[»]
4U20X-ray2.90BQ/DQ2-118[»]
4U24X-ray2.90BQ/DQ2-118[»]
4U25X-ray2.90BQ/DQ2-118[»]
4U26X-ray2.80BQ/DQ2-118[»]
4U27X-ray2.80BQ/DQ2-118[»]
4UY8electron microscopy3.80Q2-118[»]
4V47electron microscopy12.30AO2-118[»]
4V48electron microscopy11.50AO2-118[»]
4V4HX-ray3.46BQ/DQ1-118[»]
4V4QX-ray3.46BQ/DQ2-118[»]
4V4Velectron microscopy15.00BO3-117[»]
4V4Welectron microscopy15.00BO3-117[»]
4V50X-ray3.22BQ/DQ2-118[»]
4V52X-ray3.21BQ/DQ2-118[»]
4V53X-ray3.54BQ/DQ2-118[»]
4V54X-ray3.30BQ/DQ2-118[»]
4V55X-ray4.00BQ/DQ2-118[»]
4V56X-ray3.93BQ/DQ2-118[»]
4V57X-ray3.50BQ/DQ2-118[»]
4V5BX-ray3.74AQ/CQ2-118[»]
4V5Helectron microscopy5.80BQ2-118[»]
4V5YX-ray4.45BQ/DQ2-118[»]
4V64X-ray3.50BQ/DQ2-118[»]
4V65electron microscopy9.00BJ1-118[»]
4V66electron microscopy9.00BJ1-118[»]
4V69electron microscopy6.70BQ2-118[»]
4V6CX-ray3.19BQ/DQ1-118[»]
4V6DX-ray3.81BQ/DQ1-118[»]
4V6EX-ray3.71BQ/DQ1-118[»]
4V6Kelectron microscopy8.25AR1-118[»]
4V6Lelectron microscopy13.20BR1-118[»]
4V6Melectron microscopy7.10BQ2-118[»]
4V6Nelectron microscopy12.10AS2-118[»]
4V6Oelectron microscopy14.70BS2-118[»]
4V6Pelectron microscopy13.50BS2-118[»]
4V6Qelectron microscopy11.50BS2-118[»]
4V6Relectron microscopy11.50BS2-118[»]
4V6Selectron microscopy13.10AS2-118[»]
4V6Telectron microscopy8.30BQ2-118[»]
4V6Velectron microscopy9.80BU2-118[»]
4V6Yelectron microscopy12.00BQ1-118[»]
4V6Zelectron microscopy12.00BQ1-118[»]
4V70electron microscopy17.00BQ1-118[»]
4V71electron microscopy20.00BQ1-118[»]
4V72electron microscopy13.00BQ1-118[»]
4V73electron microscopy15.00BQ1-118[»]
4V74electron microscopy17.00BQ1-118[»]
4V75electron microscopy12.00BQ1-118[»]
4V76electron microscopy17.00BQ1-118[»]
4V77electron microscopy17.00BQ1-118[»]
4V78electron microscopy20.00BQ1-118[»]
4V79electron microscopy15.00BQ1-118[»]
4V7Aelectron microscopy9.00BQ1-118[»]
4V7Belectron microscopy6.80BQ1-118[»]
4V7Celectron microscopy7.60BS2-118[»]
4V7Delectron microscopy7.60AT2-118[»]
4V7Ielectron microscopy9.60AQ1-118[»]
4V7SX-ray3.25BQ/DQ2-118[»]
4V7TX-ray3.19BQ/DQ2-118[»]
4V7UX-ray3.10BQ/DQ2-118[»]
4V7VX-ray3.29BQ/DQ2-118[»]
4V85X-ray3.20U1-118[»]
4V89X-ray3.70BU1-118[»]
4V9CX-ray3.30BQ/DQ1-118[»]
4V9DX-ray3.00CQ/DQ2-118[»]
4V9OX-ray2.90AQ/CQ/EQ/GQ1-118[»]
4V9PX-ray2.90AQ/CQ/EQ/GQ1-118[»]
4WF1X-ray3.09BQ/DQ2-118[»]
4WOIX-ray3.00BQ/CQ1-118[»]
4WWWX-ray3.10RQ/YQ2-118[»]
4YBBX-ray2.10CR/DR2-118[»]
5ADYelectron microscopy4.50Q1-118[»]
5AFIelectron microscopy2.90Q1-118[»]
5AKAelectron microscopy5.70Q2-118[»]
5GADelectron microscopy3.70R1-118[»]
5GAEelectron microscopy3.33R1-118[»]
5GAFelectron microscopy4.30R2-118[»]
5GAGelectron microscopy3.80R1-118[»]
5GAHelectron microscopy3.80R1-118[»]
5H5Uelectron microscopy3.00R2-118[»]
5IQRelectron microscopy3.00Q1-118[»]
5IT8X-ray3.12CR/DR2-118[»]
5J5BX-ray2.80CR/DR2-118[»]
5J7LX-ray3.00CR/DR2-118[»]
5J88X-ray3.32CR/DR2-118[»]
5J8AX-ray3.10CR/DR2-118[»]
5J91X-ray2.96CR/DR2-118[»]
5JC9X-ray3.03CR/DR2-118[»]
5JTEelectron microscopy3.60BQ1-118[»]
5JU8electron microscopy3.60BQ1-118[»]
5KCRelectron microscopy3.601U1-118[»]
5KCSelectron microscopy3.901U1-118[»]
5KPSelectron microscopy3.90Q1-118[»]
5KPVelectron microscopy4.10P1-118[»]
5KPWelectron microscopy3.90P1-118[»]
5KPXelectron microscopy3.90P1-118[»]
5L3Pelectron microscopy3.70U1-118[»]
5LZAelectron microscopy3.60Q2-118[»]
5LZBelectron microscopy5.30Q2-118[»]
5LZCelectron microscopy4.80Q2-118[»]
5LZDelectron microscopy3.40Q2-118[»]
5LZEelectron microscopy3.50Q2-118[»]
5LZFelectron microscopy4.60Q2-118[»]
5MDVelectron microscopy2.97Q1-118[»]
5MDWelectron microscopy3.06Q1-118[»]
5MDYelectron microscopy3.35Q1-118[»]
5MDZelectron microscopy3.10Q1-118[»]
5MGPelectron microscopy3.10Q2-118[»]
5NCOelectron microscopy4.80R2-118[»]
5NP6electron microscopy3.60o2-118[»]
5NWYelectron microscopy2.93d1-118[»]
5O2Relectron microscopy3.40Q2-118[»]
5U4Ielectron microscopy3.50R1-118[»]
5U9Felectron microscopy3.20191-118[»]
5U9Gelectron microscopy3.20191-118[»]
5UYKelectron microscopy3.90192-118[»]
5UYLelectron microscopy3.60192-118[»]
5UYMelectron microscopy3.20192-118[»]
5UYNelectron microscopy4.00192-118[»]
5UYPelectron microscopy3.90192-118[»]
5UYQelectron microscopy3.80192-118[»]
5WDTelectron microscopy3.00Q2-116[»]
5WE4electron microscopy3.10Q2-116[»]
5WE6electron microscopy3.40Q2-116[»]
5WFKelectron microscopy3.40Q2-116[»]
6BU8electron microscopy3.50192-118[»]
6C4Ielectron microscopy3.24R1-118[»]
6ENFelectron microscopy3.20Q2-118[»]
6ENJelectron microscopy3.70Q2-118[»]
6ENUelectron microscopy3.10Q2-118[»]
ProteinModelPortaliP0A7L3
SMRiP0A7L3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7L3

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108YZX Bacteria
COG0292 LUCA
HOGENOMiHOG000035046
InParanoidiP0A7L3
KOiK02887
OMAiDLAMNHP
PhylomeDBiP0A7L3

Family and domain databases

CDDicd07026 Ribosomal_L20, 1 hit
Gene3Di1.10.1900.20, 1 hit
HAMAPiMF_00382 Ribosomal_L20, 1 hit
InterProiView protein in InterPro
IPR005813 Ribosomal_L20
IPR035566 Ribosomal_protein_L20_C
PANTHERiPTHR10986 PTHR10986, 1 hit
PfamiView protein in Pfam
PF00453 Ribosomal_L20, 1 hit
PRINTSiPR00062 RIBOSOMALL20
SUPFAMiSSF74731 SSF74731, 1 hit
TIGRFAMsiTIGR01032 rplT_bact, 1 hit
PROSITEiView protein in PROSITE
PS00937 RIBOSOMAL_L20, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR
60 70 80 90 100
RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVEI DRKILADIAV
110
FDKVAFTALV EKAKAALA
Length:118
Mass (Da):13,497
Last modified:January 23, 2007 - v2
Checksum:iE60AFDBB6F05DFB3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33R → A in CAA23562 (PubMed:6317865).Curated1
Sequence conflicti33R → A in AAA51468 (PubMed:6317865).Curated1

Mass spectrometryi

Molecular mass is 13366.9 Da from positions 2 - 118. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00291 Genomic DNA Translation: CAA23562.1
K02844 Genomic DNA Translation: AAA51468.1
U00096 Genomic DNA Translation: AAC74786.1
AP009048 Genomic DNA Translation: BAA15483.1
M10423 Genomic DNA Translation: AAA23960.1
PIRiD64930 R5EC20
RefSeqiNP_416231.1, NC_000913.3
WP_000124850.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74786; AAC74786; b1716
BAA15483; BAA15483; BAA15483
GeneIDi34153126
945152
KEGGiecj:JW1706
eco:b1716
PATRICifig|1411691.4.peg.541

Similar proteinsi

Entry informationi

Entry nameiRL20_ECOLI
AccessioniPrimary (citable) accession number: P0A7L3
Secondary accession number(s): P02421, Q47253
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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