Reviewed, UniProtKB/Swiss-Prot P0A7K2 (RL7_ECOLI)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L7/L12
Alternative name(s):
    L8

Gene names

Name:	rplL
Ordered Locus Names:	b3986, JW3949

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation.
Subunit structure	Part of the 50S ribosomal subunit. L7/L12 forms dimers with an elongated shape. Two dimers associate with a copy of L10 to form a very strong complex (called L8).
Post-translational modification	Acetylation of Ser-2 converts L12 to L7. Lys-82 was found to be 50% monomethylated.
Sequence similarities	Belongs to the ribosomal protein L12P family.
Mass spectrometry	Molecular mass is 12206.7 Da from positions 2 - 121. Determined by MALDI. Non-methylated. Ref.12

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Methylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
crp	P0ACJ8	1	EBI-543702,EBI-547513
kbaY	P0AB74	1	EBI-543702,EBI-543669
moaD	P30748	1	EBI-543702,EBI-554366
nikR	P0A6Z6	1	EBI-543702,EBI-562488
yccU	P75874	1	EBI-543702,EBI-544472

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 121

120

50S ribosomal protein L7/L12

PRO_0000157528

Amino acid modifications

Modified residue

2

1

N-acetylserine; in L7

Modified residue

82

1

N6-methyllysine; partial

Experimental info

Sequence conflict

6

1

D → F AA sequence Ref.9

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

121

Helix Strand Turn

Details...

Helix

5 – 12

8

Helix

17 – 30

14

Beta strand

55 – 61

7

Helix

63 – 65

3

Helix

66 – 77

12

Helix

81 – 89

9

Beta strand

92 – 99

8

Helix

101 – 114

14

Beta strand

117 – 121

5

Sequences

	Sequence		Length	Mass (Da)
	P0A7K2-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 08B051A0CDAA527E	FASTA	121	12,295
	10 20 30 40 50 60 MSITKDQIIE AVAAMSVMDV VELISAMEEK FGVSAAAAVA VAAGPVEAAE EKTEFDVILK 70 80 90 100 110 120 AAGANKVAVI KAVRGATGLG LKEAKDLVES APAALKEGVS KDDAEALKKA LEEAGAEVEV K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli." Post L.E., Strycharz G.D., Nomura M., Lewis H., Dennis P.P. Proc. Natl. Acad. Sci. U.S.A. 76:1697-1701(1979) [PubMed: 377281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence determination in 3'-end proximalely labelled DNA." Gurevich A.I., Avakov A.E. Bioorg. Khim. 5:301-304(1979) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The primary structure of an acidic protein from 50-S ribosomes of Escherichia coli which is involved in GTP hydrolysis dependent on elongation factors G and T." Terhorst C., Moeller W., Laursen R., Wittmann-Liebold B. Eur. J. Biochem. 34:138-152(1973) [PubMed: 4573678] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-121. Strain: MRE-600.
[7]	"The ribosomal protein L8 is a complex L7/L12 and L10." Pettersson I., Hardy S.J.S., Liljas A. FEBS Lett. 64:135-138(1976) [PubMed: 773698] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-121. Strain: MRE-600.
[8]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13. Strain: K12 / EMG2.
[9]	"Small genes/gene-products in Escherichia coli K-12." Wasinger V.C., Humphery-Smith I. FEMS Microbiol. Lett. 169:375-382(1998) [PubMed: 9868784] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Strain: K12.
[10]	"Protein identification with N and C-terminal sequence tags in proteome projects." Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F. J. Mol. Biol. 278:599-608(1998) [PubMed: 9600841] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-5. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[11]	"The nucleotide sequence at the proximal end of rpoB gene of Escherichia coli." Gurevich A.I., Avakov A.E., Kolosov M.N. Bioorg. Khim. 5:1735-1739(1979) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 54-121.
[12]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[13]	"Structure of the C-terminal domain of the ribosomal protein L7/L12 from Escherichia coli at 1.7 A." Leijonmarck M., Liljas A. J. Mol. Biol. 195:555-580(1987) [PubMed: 3309338] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
[14]	"Topology of the secondary structure elements of ribosomal protein L7/L12 from E. coli in solution." Bocharov E.V., Gudkov A.T., Arseniev A.S. FEBS Lett. 379:291-294(1996) [PubMed: 8603708] [Abstract] Cited for: STRUCTURE BY NMR.
[15]	"Conformational independence of N- and C-domains in ribosomal protein L7/L12 and in the complex with protein L10." Bocharov E.V., Gudkov A.T., Budovskaya E.V., Arseniev A.S. FEBS Lett. 423:347-350(1998) [PubMed: 9515737] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

V00339 Genomic DNA. Translation: CAA23624.1.
M38301 Genomic DNA. Translation: AAA24573.1.
U00006 Genomic DNA. Translation: AAC43084.1.
U00096 Genomic DNA. Translation: AAC76960.1.
AP009048 Genomic DNA. Translation: BAE77334.1.

PIR

R5EC7. S12575.

RefSeq

AP_003833.1.
NP_418413.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CTF	X-ray	1.70	A	48-121	[»]
1RQS	NMR	-	A	48-120	[»]
1RQT	NMR	-	A/B	2-37	[»]
1RQU	NMR	-	A/B	2-120	[»]
1RQV	NMR	-	A/B	2-120	[»]
2BCW	electron microscopy	11.20	B	54-120	[»]
2GYA	electron microscopy	15.00	3/5	3-120	[»]
2GYC	electron microscopy	15.00	3/5	3-121	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7K2. 124 interactions.

STRING

P0A7K2.

PTM databases

PhosSite

P0A7K2.

2-D gel databases

SWISS-2DPAGE

P0A7K2.

ECO2DBASE

A013.0. 6TH EDITION.
B013.0. 6TH EDITION.

Genome annotation databases

GeneID

948489.

GenomeReviews

Gene locus JW3949 in contig AP009048_GR.
Gene locus b3986 in contig U00096_GR.

KEGG

ecj:JW3949.
eco:b3986.

Organism-specific databases

EchoBASE

EB0866.

EcoGene

EG10873. rplL.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7K2.

OMA

KINVIKA.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10873-MON.

Gene expression databases

Genevestigator

P0A7K2.

Family and domain databases

HAMAP

MF_00368.
[Tree]

InterPro

IPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
[Graphical view]

Gene3D

G3DSA:3.30.1390.10. Ribosomal_L7/12_C/ClpS-like. 1 hit.

Pfam

PF00542. Ribosomal_L12. 1 hit.
[Graphical view]

ProDom

PD001326. Ribosomal_L12. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00855. L12. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RL7_ECOLI

Accession

Primary (citable) accession number: P0A7K2
Secondary accession number(s): P02392, Q2M8S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)