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Protein

Peptide chain release factor 3

Gene

prfC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 27GTPBy similarity8
Nucleotide bindingi88 – 92GTPBy similarity5
Nucleotide bindingi142 – 145GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: EcoCyc
  • GTPase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-HAMAP
  • translation release factor activity, codon nonspecific Source: EcoCyc
  • translation release factor activity, codon specific Source: UniProtKB-HAMAP

GO - Biological processi

  • regulation of translational termination Source: UniProtKB-HAMAP
  • translational termination Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12114-MONOMER.
ECOL316407:JW5873-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 3
Short name:
RF-3
Gene namesi
Name:prfC
Synonyms:miaD, tos
Ordered Locus Names:b4375, JW5873
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12114. prfC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00002109382 – 529Peptide chain release factor 3Add BLAST528

Proteomic databases

EPDiP0A7I4.
PaxDbiP0A7I4.
PRIDEiP0A7I4.

Interactioni

Protein-protein interaction databases

BioGridi4260996. 17 interactors.
DIPiDIP-39402N.
IntActiP0A7I4. 5 interactors.
MINTiMINT-1229875.
STRINGi511145.b4375.

Structurei

Secondary structure

1529
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 11Combined sources6
Beta strandi13 – 20Combined sources8
Helixi26 – 35Combined sources10
Beta strandi74 – 79Combined sources6
Beta strandi82 – 87Combined sources6
Helixi97 – 101Combined sources5
Helixi102 – 105Combined sources4
Beta strandi107 – 114Combined sources8
Turni115 – 117Combined sources3
Helixi121 – 130Combined sources10
Turni131 – 134Combined sources4
Beta strandi137 – 142Combined sources6
Helixi151 – 162Combined sources12
Beta strandi165 – 174Combined sources10
Helixi176 – 178Combined sources3
Beta strandi181 – 184Combined sources4
Turni185 – 188Combined sources4
Beta strandi189 – 192Combined sources4
Helixi213 – 219Combined sources7
Helixi221 – 237Combined sources17
Helixi243 – 247Combined sources5
Beta strandi250 – 256Combined sources7
Turni259 – 262Combined sources4
Helixi265 – 275Combined sources11
Beta strandi282 – 286Combined sources5
Beta strandi297 – 303Combined sources7
Beta strandi309 – 312Combined sources4
Beta strandi316 – 323Combined sources8
Beta strandi330 – 333Combined sources4
Turni334 – 336Combined sources3
Beta strandi339 – 341Combined sources3
Beta strandi364 – 367Combined sources4
Beta strandi378 – 382Combined sources5
Beta strandi391 – 393Combined sources3
Beta strandi396 – 404Combined sources9
Helixi412 – 421Combined sources10
Beta strandi426 – 430Combined sources5
Beta strandi436 – 442Combined sources7
Helixi444 – 456Combined sources13
Beta strandi461 – 464Combined sources4
Beta strandi469 – 475Combined sources7
Helixi479 – 488Combined sources10
Helixi490 – 492Combined sources3
Beta strandi493 – 495Combined sources3
Beta strandi501 – 507Combined sources7
Helixi508 – 517Combined sources10
Beta strandi521 – 528Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H5EX-ray2.80A/B1-529[»]
2O0Felectron microscopy15.50A1-529[»]
4V85X-ray3.20AW1-529[»]
4V89X-ray3.70AW1-529[»]
4V8OX-ray3.80AY1-529[»]
ProteinModelPortaliP0A7I4.
SMRiP0A7I4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7I4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 280tr-type GAdd BLAST270

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8A. Bacteria.
COG4108. LUCA.
HOGENOMiHOG000236725.
InParanoidiP0A7I4.
KOiK02837.
OMAiVIQYRLE.
PhylomeDBiP0A7I4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00072. Rel_fac_3. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR004548. PrfC.
IPR032090. RF3_C.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF16658. RF3_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 1 hit.
TIGRFAMsiTIGR00503. prfC. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSPYLQEV AKRRTFAIIS HPDAGKTTIT EKVLLFGQAI QTAGTVKGRG
60 70 80 90 100
SNQHAKSDWM EMEKQRGISI TTSVMQFPYH DCLVNLLDTP GHEDFSEDTY
110 120 130 140 150
RTLTAVDCCL MVIDAAKGVE DRTRKLMEVT RLRDTPILTF MNKLDRDIRD
160 170 180 190 200
PMELLDEVEN ELKIGCAPIT WPIGCGKLFK GVYHLYKDET YLYQSGKGHT
210 220 230 240 250
IQEVRIVKGL NNPDLDAAVG EDLAQQLRDE LELVKGASNE FDKELFLAGE
260 270 280 290 300
ITPVFFGTAL GNFGVDHMLD GLVEWAPAPM PRQTDTRTVE ASEDKFTGFV
310 320 330 340 350
FKIQANMDPK HRDRVAFMRV VSGKYEKGMK LRQVRTAKDV VISDALTFMA
360 370 380 390 400
GDRSHVEEAY PGDILGLHNH GTIQIGDTFT QGEMMKFTGI PNFAPELFRR
410 420 430 440 450
IRLKDPLKQK QLLKGLVQLS EEGAVQVFRP ISNNDLIVGA VGVLQFDVVV
460 470 480 490 500
ARLKSEYNVE AVYESVNVAT ARWVECADAK KFEEFKRKNE SQLALDGGDN
510 520
LAYIATSMVN LRLAQERYPD VQFHQTREH
Length:529
Mass (Da):59,574
Last modified:January 23, 2007 - v2
Checksum:i2A1E6AE984C6B1C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti315V → L in CAA81223 (PubMed:8016077).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17724 Genomic DNA. Translation: BAA04578.1.
Z26313 Genomic DNA. Translation: CAA81223.1.
U14003 Genomic DNA. Translation: AAA97271.1.
U00096 Genomic DNA. Translation: AAC77328.1.
AP009048 Genomic DNA. Translation: BAE78363.1.
PIRiI59305.
RefSeqiNP_418792.1. NC_000913.3.
WP_000175940.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77328; AAC77328; b4375.
BAE78363; BAE78363; BAE78363.
GeneIDi948897.
KEGGiecj:JW5873.
eco:b4375.
PATRICi32124360. VBIEscCol129921_4520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17724 Genomic DNA. Translation: BAA04578.1.
Z26313 Genomic DNA. Translation: CAA81223.1.
U14003 Genomic DNA. Translation: AAA97271.1.
U00096 Genomic DNA. Translation: AAC77328.1.
AP009048 Genomic DNA. Translation: BAE78363.1.
PIRiI59305.
RefSeqiNP_418792.1. NC_000913.3.
WP_000175940.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H5EX-ray2.80A/B1-529[»]
2O0Felectron microscopy15.50A1-529[»]
4V85X-ray3.20AW1-529[»]
4V89X-ray3.70AW1-529[»]
4V8OX-ray3.80AY1-529[»]
ProteinModelPortaliP0A7I4.
SMRiP0A7I4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260996. 17 interactors.
DIPiDIP-39402N.
IntActiP0A7I4. 5 interactors.
MINTiMINT-1229875.
STRINGi511145.b4375.

Proteomic databases

EPDiP0A7I4.
PaxDbiP0A7I4.
PRIDEiP0A7I4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77328; AAC77328; b4375.
BAE78363; BAE78363; BAE78363.
GeneIDi948897.
KEGGiecj:JW5873.
eco:b4375.
PATRICi32124360. VBIEscCol129921_4520.

Organism-specific databases

EchoBASEiEB2037.
EcoGeneiEG12114. prfC.

Phylogenomic databases

eggNOGiENOG4105C8A. Bacteria.
COG4108. LUCA.
HOGENOMiHOG000236725.
InParanoidiP0A7I4.
KOiK02837.
OMAiVIQYRLE.
PhylomeDBiP0A7I4.

Enzyme and pathway databases

BioCyciEcoCyc:EG12114-MONOMER.
ECOL316407:JW5873-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A7I4.
PROiP0A7I4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00072. Rel_fac_3. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR004548. PrfC.
IPR032090. RF3_C.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF16658. RF3_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 1 hit.
TIGRFAMsiTIGR00503. prfC. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRF3_ECOLI
AccessioniPrimary (citable) accession number: P0A7I4
Secondary accession number(s): P33998, Q2M5U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.