Reviewed, UniProtKB/Swiss-Prot P0A7I4 (RF3_ECOLI)

Names and origin

Protein names

Recommended name:
    Peptide chain release factor 3
      Short name=RF-3

Gene names

Name:	prfC
Synonyms:	miaD, tos
Ordered Locus Names:	b4375, JW5873

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP. HAMAP MF_00072
Subcellular location	Cytoplasm. HAMAP MF_00072
Sequence similarities	Belongs to the GTP-binding elongation factor family. PrfC subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	regulation of translational termination Inferred from electronic annotation. Source: HAMAP translational termination Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pfkA	P0A796	1	EBI-556252,EBI-554405

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.2 Ref.6

Chain

2 – 529

528

Peptide chain release factor 3 HAMAP MF_00072

PRO_0000210938

Regions

Nucleotide binding

20 – 27

8

GTP By similarity

Nucleotide binding

88 – 92

5

GTP By similarity

Nucleotide binding

142 – 145

4

GTP By similarity

Experimental info

Sequence conflict

315

1

V → L in CAA81223. Ref.2

Secondary structure

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529

Helix Strand Turn

Details...

Helix

5 – 11

7

Beta strand

13 – 19

7

Helix

26 – 36

11

Beta strand

74 – 79

6

Beta strand

82 – 87

6

Helix

97 – 102

6

Helix

103 – 105

3

Beta strand

107 – 114

8

Turn

115 – 117

3

Helix

121 – 130

10

Turn

131 – 134

4

Beta strand

137 – 142

6

Helix

151 – 162

12

Beta strand

165 – 174

10

Helix

176 – 178

3

Beta strand

181 – 184

4

Turn

185 – 188

4

Beta strand

189 – 192

4

Beta strand

198 – 200

3

Beta strand

205 – 207

3

Helix

213 – 237

25

Helix

243 – 248

6

Beta strand

250 – 256

7

Turn

259 – 262

4

Helix

265 – 275

11

Beta strand

282 – 286

5

Beta strand

297 – 303

7

Beta strand

316 – 323

8

Beta strand

330 – 333

4

Turn

334 – 337

4

Beta strand

338 – 341

4

Beta strand

364 – 367

4

Beta strand

378 – 382

5

Beta strand

391 – 393

3

Beta strand

396 – 403

8

Helix

412 – 421

10

Beta strand

422 – 424

3

Beta strand

426 – 430

5

Beta strand

436 – 442

7

Helix

444 – 455

12

Beta strand

461 – 464

4

Beta strand

469 – 475

7

Helix

479 – 488

10

Helix

490 – 492

3

Beta strand

493 – 495

3

Beta strand

501 – 507

7

Helix

508 – 517

10

Beta strand

521 – 528

8

Sequences

	Sequence		Length	Mass (Da)
	P0A7I4-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2A1E6AE984C6B1C6	FASTA	529	59,574
	10 20 30 40 50 60 MTLSPYLQEV AKRRTFAIIS HPDAGKTTIT EKVLLFGQAI QTAGTVKGRG SNQHAKSDWM 70 80 90 100 110 120 EMEKQRGISI TTSVMQFPYH DCLVNLLDTP GHEDFSEDTY RTLTAVDCCL MVIDAAKGVE 130 140 150 160 170 180 DRTRKLMEVT RLRDTPILTF MNKLDRDIRD PMELLDEVEN ELKIGCAPIT WPIGCGKLFK 190 200 210 220 230 240 GVYHLYKDET YLYQSGKGHT IQEVRIVKGL NNPDLDAAVG EDLAQQLRDE LELVKGASNE 250 260 270 280 290 300 FDKELFLAGE ITPVFFGTAL GNFGVDHMLD GLVEWAPAPM PRQTDTRTVE ASEDKFTGFV 310 320 330 340 350 360 FKIQANMDPK HRDRVAFMRV VSGKYEKGMK LRQVRTAKDV VISDALTFMA GDRSHVEEAY 370 380 390 400 410 420 PGDILGLHNH GTIQIGDTFT QGEMMKFTGI PNFAPELFRR IRLKDPLKQK QLLKGLVQLS 430 440 450 460 470 480 EEGAVQVFRP ISNNDLIVGA VGVLQFDVVV ARLKSEYNVE AVYESVNVAT ARWVECADAK 490 500 510 520 KFEEFKRKNE SQLALDGGDN LAYIATSMVN LRLAQERYPD VQFHQTREH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the prfC gene, which encodes peptide-chain-release factor 3 of Escherichia coli." Mikuni O., Ito K., Mofatt J., Matsumura K., McCaughan K., Nobukuni T., Tate W., Nakamura Y. Proc. Natl. Acad. Sci. U.S.A. 91:5798-5802(1994) [PubMed: 8016068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-10. Strain: K12.
[2]	"Localization and characterization of the gene encoding release factor RF3 in Escherichia coli." Grentzmann G., Brechemier-Baey D., Heurgue V., Mora L., Buckingham R.H. Proc. Natl. Acad. Sci. U.S.A. 91:5848-5852(1994) [PubMed: 8016077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Osmo-expression and fast two-step purification of Escherichia coli translation termination factor RF-3." Mortensen K.K., Hansen H.F., Grentzmann G., Buckingham R.H., Sperling-Petersen H.U. Eur. J. Biochem. 234:732-736(1995) [PubMed: 8575429] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-19.
[7]	"Function of polypeptide chain release factor RF-3 in Escherichia coli. RF-3 action in termination is predominantly at UGA-containing stop signals." Grentzmann G., Brechemier-Baey D., Heurgue-Hamard V., Buckingham R.H. J. Biol. Chem. 270:10595-10600(1995) [PubMed: 7737996] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D17724 Genomic DNA. Translation: BAA04578.1.
Z26313 Genomic DNA. Translation: CAA81223.1.
U14003 Genomic DNA. Translation: AAA97271.1.
U00096 Genomic DNA. Translation: AAC77328.1.
AP009048 Genomic DNA. Translation: BAE78363.1.

PIR

I59305.

RefSeq

AP_004862.1.
NP_418792.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H5E	X-ray	2.80	A/B	1-529	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7I4. 5 interactions.

Genome annotation databases

GeneID

948897.

GenomeReviews

Gene locus JW5873 in contig AP009048_GR.
Gene locus b4375 in contig U00096_GR.

KEGG

ecj:JW5873.
eco:b4375.

Organism-specific databases

EchoBASE

EB2037.

EcoGene

EG12114. prfC.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7I4.

OMA

P0A7I4. LMEVCRM.

Enzyme and pathway databases

BioCyc

EcoCyc:EG12114-MON.

Family and domain databases

HAMAP

MF_00072.
[Tree]

InterPro

IPR004548. PrfC.
IPR000795. ProtSyn_GTP_bd.
IPR005225. Small_GTP_bd.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]

Pfam

PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]

PRINTS

PR00315. ELONGATNFCT.

TIGRFAMs

TIGR00503. prfC. 1 hit.
TIGR00231. small_GTP. 1 hit.

PROSITE

PS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RF3_ECOLI

Accession

Primary (citable) accession number: P0A7I4
Secondary accession number(s): P33998, Q2M5U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)