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P0A7I4 (RF3_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide chain release factor 3

Short name=RF-3
Gene names
Name:prfC
Synonyms:miaD, tos
Ordered Locus Names:b4375, JW5873
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP. HAMAP-Rule MF_00072

Subcellular location

Cytoplasm HAMAP-Rule MF_00072.

Sequence similarities

Belongs to the GTP-binding elongation factor family. PrfC subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

pfkAP0A7961EBI-556252,EBI-554405

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.2 Ref.6
Chain2 – 529528Peptide chain release factor 3 HAMAP-Rule MF_00072
PRO_0000210938

Regions

Nucleotide binding20 – 278GTP By similarity
Nucleotide binding88 – 925GTP By similarity
Nucleotide binding142 – 1454GTP By similarity

Experimental info

Sequence conflict3151V → L in CAA81223. Ref.2

Secondary structure

............................................................................ 529
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A7I4 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 2A1E6AE984C6B1C6

FASTA52959,574
        10         20         30         40         50         60 
MTLSPYLQEV AKRRTFAIIS HPDAGKTTIT EKVLLFGQAI QTAGTVKGRG SNQHAKSDWM 

        70         80         90        100        110        120 
EMEKQRGISI TTSVMQFPYH DCLVNLLDTP GHEDFSEDTY RTLTAVDCCL MVIDAAKGVE 

       130        140        150        160        170        180 
DRTRKLMEVT RLRDTPILTF MNKLDRDIRD PMELLDEVEN ELKIGCAPIT WPIGCGKLFK 

       190        200        210        220        230        240 
GVYHLYKDET YLYQSGKGHT IQEVRIVKGL NNPDLDAAVG EDLAQQLRDE LELVKGASNE 

       250        260        270        280        290        300 
FDKELFLAGE ITPVFFGTAL GNFGVDHMLD GLVEWAPAPM PRQTDTRTVE ASEDKFTGFV 

       310        320        330        340        350        360 
FKIQANMDPK HRDRVAFMRV VSGKYEKGMK LRQVRTAKDV VISDALTFMA GDRSHVEEAY 

       370        380        390        400        410        420 
PGDILGLHNH GTIQIGDTFT QGEMMKFTGI PNFAPELFRR IRLKDPLKQK QLLKGLVQLS 

       430        440        450        460        470        480 
EEGAVQVFRP ISNNDLIVGA VGVLQFDVVV ARLKSEYNVE AVYESVNVAT ARWVECADAK 

       490        500        510        520 
KFEEFKRKNE SQLALDGGDN LAYIATSMVN LRLAQERYPD VQFHQTREH 

« Hide

References

« Hide 'large scale' references
[1]"Identification of the prfC gene, which encodes peptide-chain-release factor 3 of Escherichia coli."
Mikuni O., Ito K., Mofatt J., Matsumura K., McCaughan K., Nobukuni T., Tate W., Nakamura Y.
Proc. Natl. Acad. Sci. U.S.A. 91:5798-5802(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-10.
Strain: K12.
[2]"Localization and characterization of the gene encoding release factor RF3 in Escherichia coli."
Grentzmann G., Brechemier-Baey D., Heurgue V., Mora L., Buckingham R.H.
Proc. Natl. Acad. Sci. U.S.A. 91:5848-5852(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Osmo-expression and fast two-step purification of Escherichia coli translation termination factor RF-3."
Mortensen K.K., Hansen H.F., Grentzmann G., Buckingham R.H., Sperling-Petersen H.U.
Eur. J. Biochem. 234:732-736(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-19.
[7]"Function of polypeptide chain release factor RF-3 in Escherichia coli. RF-3 action in termination is predominantly at UGA-containing stop signals."
Grentzmann G., Brechemier-Baey D., Heurgue-Hamard V., Buckingham R.H.
J. Biol. Chem. 270:10595-10600(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17724 Genomic DNA. Translation: BAA04578.1.
Z26313 Genomic DNA. Translation: CAA81223.1.
U14003 Genomic DNA. Translation: AAA97271.1.
U00096 Genomic DNA. Translation: AAC77328.1.
AP009048 Genomic DNA. Translation: BAE78363.1.
PIRI59305.
RefSeqNP_418792.1. NC_000913.3.
YP_492504.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2H5EX-ray2.80A/B1-529[»]
2O0Felectron microscopy15.50A1-529[»]
3SFSX-ray3.20W1-529[»]
3UOQX-ray3.70W1-529[»]
3ZVOX-ray3.80Y1-529[»]
ProteinModelPortalP0A7I4.
SMRP0A7I4. Positions 3-527.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-39402N.
IntActP0A7I4. 5 interactions.
MINTMINT-1229875.
STRING511145.b4375.

Proteomic databases

PaxDbP0A7I4.
PRIDEP0A7I4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77328; AAC77328; b4375.
BAE78363; BAE78363; BAE78363.
GeneID12932002.
948897.
KEGGecj:Y75_p4258.
eco:b4375.
PATRIC32124360. VBIEscCol129921_4520.

Organism-specific databases

EchoBASEEB2037.
EcoGeneEG12114. prfC.

Phylogenomic databases

eggNOGCOG4108.
HOGENOMHOG000236725.
KOK02837.
OMAFHKTREH.
OrthoDBEOG6677Q1.
PhylomeDBP0A7I4.

Enzyme and pathway databases

BioCycEcoCyc:EG12114-MONOMER.
ECOL316407:JW5873-MONOMER.

Gene expression databases

GenevestigatorP0A7I4.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00072. Rel_fac_3.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR027417. P-loop_NTPase.
IPR004548. PrfC.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 1 hit.
TIGRFAMsTIGR00503. prfC. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0A7I4.
PROP0A7I4.

Entry information

Entry nameRF3_ECOLI
AccessionPrimary (citable) accession number: P0A7I4
Secondary accession number(s): P33998, Q2M5U3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene