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Protein

Peptide chain release factor 1

Gene

prfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.

GO - Molecular functioni

  • ribosome binding Source: EcoCyc
  • translation release factor activity, codon specific Source: EcoCyc

GO - Biological processi

  • translational termination Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10761-MONOMER.
ECOL316407:JW1202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 1
Short name:
RF-1
Gene namesi
Name:prfA
Synonyms:sueB, uar
Ordered Locus Names:b1211, JW1202
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10761. prfA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi235Q → E or A: Loss of methylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001776681 – 360Peptide chain release factor 1Add BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei235N5-methylglutamine2 Publications1

Post-translational modificationi

Methylated by PrmC. Methylation increases the termination efficiency of RF1.3 Publications

Keywords - PTMi

Methylation

Proteomic databases

EPDiP0A7I0.
PaxDbiP0A7I0.
PRIDEiP0A7I0.

Interactioni

Protein-protein interaction databases

BioGridi4260107. 6 interactors.
DIPiDIP-35936N.
IntActiP0A7I0. 13 interactors.
MINTiMINT-1219241.
STRINGi511145.b1211.

Structurei

Secondary structure

1360
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 26Combined sources17
Helixi35 – 55Combined sources21
Helixi106 – 108Combined sources3
Beta strandi111 – 117Combined sources7
Beta strandi119 – 121Combined sources3
Helixi122 – 141Combined sources20
Beta strandi148 – 153Combined sources6
Beta strandi157 – 166Combined sources10
Beta strandi168 – 170Combined sources3
Helixi172 – 175Combined sources4
Helixi176 – 178Combined sources3
Beta strandi180 – 186Combined sources7
Beta strandi197 – 204Combined sources8
Turni211 – 213Combined sources3
Beta strandi219 – 226Combined sources8
Turni234 – 236Combined sources3
Beta strandi240 – 248Combined sources9
Turni249 – 251Combined sources3
Beta strandi254 – 261Combined sources8
Helixi263 – 278Combined sources16
Turni279 – 284Combined sources6
Beta strandi306 – 311Combined sources6
Turni312 – 315Combined sources4
Beta strandi316 – 319Combined sources4
Helixi320 – 322Combined sources3
Beta strandi324 – 327Combined sources4
Helixi329 – 334Combined sources6
Helixi338 – 352Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B3TX-ray3.10B1-356[»]
5J30X-ray3.20QY/XY1-360[»]
5J3CX-ray3.04QY/XY1-360[»]
5J4DX-ray3.10JA/OC1-360[»]
ProteinModelPortaliP0A7I0.
SMRiP0A7I0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7I0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8K. Bacteria.
COG0216. LUCA.
HOGENOMiHOG000074815.
InParanoidiP0A7I0.
KOiK02835.
OMAiWRKVQDD.
PhylomeDBiP0A7I0.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1. 1 hit.
InterProiIPR014720. dsRBD_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A7I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSIVAKLE ALHERHEEVQ ALLGDAQTIA DQERFRALSR EYAQLSDVSR
60 70 80 90 100
CFTDWQQVQE DIETAQMMLD DPEMREMAQD ELREAKEKSE QLEQQLQVLL
110 120 130 140 150
LPKDPDDERN AFLEVRAGTG GDEAALFAGD LFRMYSRYAE ARRWRVEIMS
160 170 180 190 200
ASEGEHGGYK EIIAKISGDG VYGRLKFESG GHRVQRVPAT ESQGRIHTSA
210 220 230 240 250
CTVAVMPELP DAELPDINPA DLRIDTFRSS GAGGQHVNTT DSAIRITHLP
260 270 280 290 300
TGIVVECQDE RSQHKNKAKA LSVLGARIHA AEMAKRQQAE ASTRRNLLGS
310 320 330 340 350
GDRSDRNRTY NFPQGRVTDH RINLTLYRLD EVMEGKLDML IEPIIQEHQA
360
DQLAALSEQE
Length:360
Mass (Da):40,517
Last modified:June 7, 2005 - v1
Checksum:i47B3B77705A71954
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 91PEMRE…EKSEQ → LRYLLYLRLFVVLFRHVVGD (PubMed:2684779).CuratedAdd BLAST20
Sequence conflicti211D → E in AAC43437 (PubMed:7543480).Curated1
Sequence conflicti241D → G in AAA24519 (PubMed:3889910).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11519 Genomic DNA. Translation: AAA24519.1.
U18555 Genomic DNA. Translation: AAC43437.1.
U00096 Genomic DNA. Translation: AAC74295.1.
AP009048 Genomic DNA. Translation: BAA36069.1.
M25323 Genomic DNA. Translation: AAA23955.1.
D28567 Genomic DNA. Translation: BAA05914.1.
PIRiH64867. FCECR1.
RefSeqiNP_415729.1. NC_000913.3.
WP_000804726.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74295; AAC74295; b1211.
BAA36069; BAA36069; BAA36069.
GeneIDi949002.
KEGGiecj:JW1202.
eco:b1211.
PATRICi32117674. VBIEscCol129921_1259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11519 Genomic DNA. Translation: AAA24519.1.
U18555 Genomic DNA. Translation: AAC43437.1.
U00096 Genomic DNA. Translation: AAC74295.1.
AP009048 Genomic DNA. Translation: BAA36069.1.
M25323 Genomic DNA. Translation: AAA23955.1.
D28567 Genomic DNA. Translation: BAA05914.1.
PIRiH64867. FCECR1.
RefSeqiNP_415729.1. NC_000913.3.
WP_000804726.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B3TX-ray3.10B1-356[»]
5J30X-ray3.20QY/XY1-360[»]
5J3CX-ray3.04QY/XY1-360[»]
5J4DX-ray3.10JA/OC1-360[»]
ProteinModelPortaliP0A7I0.
SMRiP0A7I0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260107. 6 interactors.
DIPiDIP-35936N.
IntActiP0A7I0. 13 interactors.
MINTiMINT-1219241.
STRINGi511145.b1211.

Proteomic databases

EPDiP0A7I0.
PaxDbiP0A7I0.
PRIDEiP0A7I0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74295; AAC74295; b1211.
BAA36069; BAA36069; BAA36069.
GeneIDi949002.
KEGGiecj:JW1202.
eco:b1211.
PATRICi32117674. VBIEscCol129921_1259.

Organism-specific databases

EchoBASEiEB0754.
EcoGeneiEG10761. prfA.

Phylogenomic databases

eggNOGiENOG4105C8K. Bacteria.
COG0216. LUCA.
HOGENOMiHOG000074815.
InParanoidiP0A7I0.
KOiK02835.
OMAiWRKVQDD.
PhylomeDBiP0A7I0.

Enzyme and pathway databases

BioCyciEcoCyc:EG10761-MONOMER.
ECOL316407:JW1202-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A7I0.
PROiP0A7I0.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1. 1 hit.
InterProiIPR014720. dsRBD_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRF1_ECOLI
AccessioniPrimary (citable) accession number: P0A7I0
Secondary accession number(s): P07011, P77340
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 7, 2005
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.