Reviewed, UniProtKB/Swiss-Prot P0A7I0 (RF1_ECOLI)

Names and origin

Protein names

Recommended name:
    Peptide chain release factor 1
      Short name=RF-1

Gene names

Name:	prfA
Synonyms:	sueB, uar
Ordered Locus Names:	b1211, JW1202

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. HAMAP MF_00093
Subcellular location	Cytoplasm. HAMAP MF_00093
Post-translational modification	Methylated by hemK. Ref.10
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	translational termination Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 360

360

Peptide chain release factor 1 HAMAP MF_00093

PRO_0000177668

Amino acid modifications

Modified residue

235

1

N5-methylglutamine Ref.10

Experimental info

Sequence conflict

72 – 91

20

PEMRE…EKSEQ → LRYLLYLRLFVVLFRHVVGD Ref.7

Sequence conflict

211

1

D → E in AAC43437. Ref.3

Sequence conflict

241

1

D → G in AAA24519. Ref.1

Secondary structure

1

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360

Helix Strand Turn

Details...

Helix

10 – 26

17

Helix

35 – 55

21

Helix

106 – 108

3

Beta strand

111 – 117

7

Beta strand

119 – 121

3

Helix

122 – 141

20

Beta strand

148 – 153

6

Beta strand

157 – 166

10

Beta strand

168 – 170

3

Helix

172 – 175

4

Helix

176 – 178

3

Beta strand

180 – 186

7

Beta strand

197 – 204

8

Turn

211 – 213

3

Beta strand

219 – 226

8

Turn

234 – 236

3

Beta strand

240 – 248

9

Turn

249 – 251

3

Beta strand

254 – 261

8

Helix

263 – 278

16

Turn

279 – 284

6

Beta strand

306 – 311

6

Turn

312 – 315

4

Beta strand

316 – 319

4

Helix

320 – 322

3

Beta strand

324 – 327

4

Helix

329 – 334

6

Helix

338 – 352

15

Sequences

	Sequence		Length	Mass (Da)
	P0A7I0-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 47B3B77705A71954	FASTA	360	40,517
	10 20 30 40 50 60 MKPSIVAKLE ALHERHEEVQ ALLGDAQTIA DQERFRALSR EYAQLSDVSR CFTDWQQVQE 70 80 90 100 110 120 DIETAQMMLD DPEMREMAQD ELREAKEKSE QLEQQLQVLL LPKDPDDERN AFLEVRAGTG 130 140 150 160 170 180 GDEAALFAGD LFRMYSRYAE ARRWRVEIMS ASEGEHGGYK EIIAKISGDG VYGRLKFESG 190 200 210 220 230 240 GHRVQRVPAT ESQGRIHTSA CTVAVMPELP DAELPDINPA DLRIDTFRSS GAGGQHVNTT 250 260 270 280 290 300 DSAIRITHLP TGIVVECQDE RSQHKNKAKA LSVLGARIHA AEMAKRQQAE ASTRRNLLGS 310 320 330 340 350 360 GDRSDRNRTY NFPQGRVTDH RINLTLYRLD EVMEGKLDML IEPIIQEHQA DQLAALSEQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacterial peptide chain release factors: conserved primary structure and possible frameshift regulation of release factor 2." Craigen W.J., Cook R.G., Tate W.P., Caskey C.T. Proc. Natl. Acad. Sci. U.S.A. 82:3616-3620(1985) [PubMed: 3889910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Rapid and precise mapping of the Escherichia coli release factor genes by two physical approaches." Lee C.C., Kohara Y., Akiyama K., Smith C.L., Craigen W.J., Caskey C.T. J. Bacteriol. 170:4537-4541(1988) [PubMed: 3049538] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12." Strohmaier H., Remler P., Renner W., Hoegenauer G. J. Bacteriol. 177:4488-4500(1995) [PubMed: 7543480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Cloning and structure of the hem A gene of Escherichia coli K-12." Li J.-M., Russell C.S., Cosloy S.D. Gene 82:209-217(1989) [PubMed: 2684779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-91. Strain: K12.
[8]	"Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene." Verkamp E., Chelm B.K. J. Bacteriol. 171:4728-4735(1989) [PubMed: 2548996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6. Strain: K12.
[9]	"Cloning and sequencing of a previously unidentified gene that is involved in the biosynthesis of heme in Escherichia coli." Nakayashiki T., Nishimura K., Inokuchi H. Gene 153:67-70(1995) [PubMed: 7883187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 339-360. Strain: K12.
[10]	"A post-translational modification in the GGQ motif of RF2 from Escherichia coli stimulates termination of translation." Dincbas-Renqvist V., Engstroem A., Mora L., Heurgue-Hamard V., Buckingham R., Ehrenberg M. EMBO J. 19:6900-6907(2000) [PubMed: 11118225] [Abstract] Cited for: METHYLATION AT GLN-235.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M11519 Genomic DNA. Translation: AAA24519.1.
U18555 Genomic DNA. Translation: AAC43437.1.
U00096 Genomic DNA. Translation: AAC74295.1.
AP009048 Genomic DNA. Translation: BAA36069.1.
M25323 Genomic DNA. Translation: AAA23955.1.
D28567 Genomic DNA. Translation: BAA05914.1.

PIR

FCECR1. H64867.

RefSeq

AP_001836.1.
NP_415729.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B3T	X-ray	3.10	B	1-356	[»]

ModBase

Search...

2-D gel databases

ECO2DBASE

H021.6. 6TH EDITION.
H021.7. 6TH EDITION.

Genome annotation databases

GeneID

949002.

GenomeReviews

Gene locus JW1202 in contig AP009048_GR.
Gene locus b1211 in contig U00096_GR.

KEGG

ecj:JW1202.
eco:b1211.

Organism-specific databases

EchoBASE

EB0754.

EcoGene

EG10761. prfA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7I0.

OMA

P0A7I0. RAYSREY.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10761-MON.

Family and domain databases

HAMAP

MF_00093.
[Tree]

InterPro

IPR005139. PCRF.
IPR000352. Pep_rel_factor_I.
IPR004373. PrfA.
[Graphical view]

PANTHER

PTHR11075:SF9. PrfA. 1 hit.

Pfam

PF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00019. prfA. 1 hit.

PROSITE

PS00745. RF_PROK_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RF1_ECOLI

Accession

Primary (citable) accession number: P0A7I0
Secondary accession number(s): P07011, P77340

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)