Reviewed,
UniProtKB/Swiss-Prot P0A7H0 (RECF_ECOLI)
Last modified
November 3, 2009.
Version 42.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DNA replication and repair protein recF | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 357 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. HAMAP MF_00365 |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the recF family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair DNA replication SOS response |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding DNA-binding Nucleotide-binding |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | DNA repair Inferred from electronic annotation. Source: HAMAP DNA replicationInferred from electronic annotation. Source: HAMAP SOS responseInferred from electronic annotation. Source: HAMAP |
| Cellular component | chromosome Inferred from electronic annotation. Source: InterPro cytoplasmInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: HAMAP protein bindingInferred from physical interaction. Source: IntAct single-stranded DNA bindingInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.6 | ||||||
| Chain | 2 – 357 | 356 | DNA replication and repair protein recF HAMAP MF_00365 | PRO_0000196414 | |||||
Regions | |||||||||
| Nucleotide binding | 30 – 37 | 8 | ATP Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 36 | 1 | K → R: Weakly active. Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular analysis of the recF gene of Escherichia coli." Blanar M.A., Sandler S.J., Armengod M.-E., Ream L.W., Clark A.J. Proc. Natl. Acad. Sci. U.S.A. 81:4622-4626(1984) [PubMed: 6379647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "DNA sequence and transcription of the region upstream of the E. coli gyrB gene." Adachi T., Mizuuchi K., Menzel R., Gellert M. Nucleic Acids Res. 12:6389-6395(1984) [PubMed: 6089112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [3] | "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [5] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [6] | "Purification and preliminary characterization of the Escherichia coli K-12 recF protein." Griffin T.J. IV, Kolodner R.D. J. Bacteriol. 172:6291-6299(1990) [PubMed: 2228960] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13, DNA-BINDING. |
| [7] | "Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop." Sandler S.J., Chackerian B., Li J.T., Clark A.J. Nucleic Acids Res. 20:839-845(1992) [PubMed: 1542576] [Abstract] Cited for: MUTAGENESIS OF LYS-36. |
Cross-references
Sequence databases | |
|---|---|
| K02179 Genomic DNA. Translation: AAA24511.1. X04341 Genomic DNA. Translation: CAA27870.1. L10328 Genomic DNA. Translation: AAA62051.1. U00096 Genomic DNA. Translation: AAC76723.1. AP009048 Genomic DNA. Translation: BAE77594.1. | |
| PIR | RQECF. A03547. |
| RefSeq | AP_004093.1. NP_418155.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1F2T based on UniProtKB P58301. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P0A7H0. 12 interactions. |
| STRING | P0A7H0. |
Proteomic databases | |
| PRIDE | P0A7H0. |
Genome annotation databases | |
| GeneID | 948209. |
| GenomeReviews | Gene locus JW3677 in contig AP009048_GR. Gene locus b3700 in contig U00096_GR. |
| KEGG | ecj:JW3677. eco:b3700. |
Organism-specific databases | |
| EchoBASE | EB0821. |
| EcoGene | EG10828. recF. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P0A7H0. |
| OMA | GQQRTLV. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:EG10828-MON. MetaCyc:EG10828-MON. |
Gene expression databases | |
| Genevestigator | P0A7H0. |
Family and domain databases | |
| HAMAP | MF_00365. [Tree] |
| InterPro | IPR001238. DNA-binding_RecF. IPR018078. DNA-binding_RecF_CS. IPR003395. RecF/RecN/SMC_N. [Graphical view] |
| Pfam | PF02463. SMC_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00611. recf. 1 hit. |
| PROSITE | PS00617. RECF_1. 1 hit. PS00618. RECF_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RECF_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0A7H0 Secondary accession number(s): P03016, Q2M812 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


