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Protein

DNA replication and repair protein RecF

Gene

recF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 37ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • DNA synthesis involved in DNA repair Source: EcoliWiki
  • double-strand break repair Source: GO_Central
  • response to UV Source: EcoliWiki
  • SOS response Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair, DNA replication, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10828-MONOMER.
MetaCyc:EG10828-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication and repair protein RecF
Gene namesi
Name:recF
Synonyms:uvrF
Ordered Locus Names:b3700, JW3677
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10828. recF.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36K → R: Weakly active. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001964142 – 357DNA replication and repair protein RecFAdd BLAST356

Proteomic databases

PaxDbiP0A7H0.
PRIDEiP0A7H0.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F53EBI-556839,EBI-543750

Protein-protein interaction databases

BioGridi4261540. 197 interactors.
DIPiDIP-36037N.
IntActiP0A7H0. 13 interactors.
MINTiMINT-1291712.
STRINGi316385.ECDH10B_3886.

Structurei

3D structure databases

ProteinModelPortaliP0A7H0.
SMRiP0A7H0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecF family.Curated

Phylogenomic databases

eggNOGiENOG4105C3X. Bacteria.
COG1195. LUCA.
HOGENOMiHOG000269560.
InParanoidiP0A7H0.
KOiK03629.
PhylomeDBiP0A7H0.

Family and domain databases

HAMAPiMF_00365. RecF. 1 hit.
InterProiView protein in InterPro
IPR001238. DNA-binding_RecF.
IPR018078. DNA-binding_RecF_CS.
IPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
PANTHERiPTHR32182:SF15. PTHR32182:SF15. 1 hit.
PfamiView protein in Pfam
PF02463. SMC_N. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00611. recf. 1 hit.
PROSITEiView protein in PROSITE
PS00617. RECF_1. 1 hit.
PS00618. RECF_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLTRLLIRD FRNIETADLA LSPGFNFLVG ANGSGKTSVL EAIYTLGHGR
60 70 80 90 100
AFRSLQIGRV IRHEQEAFVL HGRLQGEERE TAIGLTKDKQ GDSKVRIDGT
110 120 130 140 150
DGHKVAELAH LMPMQLITPE GFTLLNGGPK YRRAFLDWGC FHNEPGFFTA
160 170 180 190 200
WSNLKRLLKQ RNAALRQVTR YEQLRPWDKE LIPLAEQIST WRAEYSAGIA
210 220 230 240 250
ADMADTCKQF LPEFSLTFSF QRGWEKETEY AEVLERNFER DRQLTYTAHG
260 270 280 290 300
PHKADLRIRA DGAPVEDTLS RGQLKLLMCA LRLAQGEFLT RESGRRCLYL
310 320 330 340 350
IDDFASELDD ERRGLLASRL KATQSQVFVS AISAEHVIDM SDENSKMFTV

EKGKITD
Length:357
Mass (Da):40,514
Last modified:January 23, 2007 - v2
Checksum:iECB395B963C5DDB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02179 Genomic DNA. Translation: AAA24511.1.
X04341 Genomic DNA. Translation: CAA27870.1.
L10328 Genomic DNA. Translation: AAA62051.1.
U00096 Genomic DNA. Translation: AAC76723.1.
AP009048 Genomic DNA. Translation: BAE77594.1.
PIRiA03547. RQECF.
RefSeqiNP_418155.1. NC_000913.3.
WP_000060112.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76723; AAC76723; b3700.
BAE77594; BAE77594; BAE77594.
GeneIDi948209.
KEGGiecj:JW3677.
eco:b3700.
PATRICifig|1411691.4.peg.3003.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRECF_ECOLI
AccessioniPrimary (citable) accession number: P0A7H0
Secondary accession number(s): P03016, Q2M812
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families