Reviewed, UniProtKB/Swiss-Prot P0A7H0 (RECF_ECOLI)

Names and origin

Protein names

Recommended name:
    DNA replication and repair protein recF

Gene names

Name:	recF
Synonyms:	uvrF
Ordered Locus Names:	b3700, JW3677

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. HAMAP MF_00365
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the recF family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct single-stranded DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-556839,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 357	356	DNA replication and repair protein recF HAMAP MF_00365		PRO_0000196414
Regions
	Nucleotide binding	30 – 37	8	ATP Potential
Experimental info
	Mutagenesis	36	1	K → R: Weakly active. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P0A7H0-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: ECB395B963C5DDB9	FASTA	357	40,514
	10 20 30 40 50 60 MSLTRLLIRD FRNIETADLA LSPGFNFLVG ANGSGKTSVL EAIYTLGHGR AFRSLQIGRV 70 80 90 100 110 120 IRHEQEAFVL HGRLQGEERE TAIGLTKDKQ GDSKVRIDGT DGHKVAELAH LMPMQLITPE 130 140 150 160 170 180 GFTLLNGGPK YRRAFLDWGC FHNEPGFFTA WSNLKRLLKQ RNAALRQVTR YEQLRPWDKE 190 200 210 220 230 240 LIPLAEQIST WRAEYSAGIA ADMADTCKQF LPEFSLTFSF QRGWEKETEY AEVLERNFER 250 260 270 280 290 300 DRQLTYTAHG PHKADLRIRA DGAPVEDTLS RGQLKLLMCA LRLAQGEFLT RESGRRCLYL 310 320 330 340 350 IDDFASELDD ERRGLLASRL KATQSQVFVS AISAEHVIDM SDENSKMFTV EKGKITD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of the recF gene of Escherichia coli." Blanar M.A., Sandler S.J., Armengod M.-E., Ream L.W., Clark A.J. Proc. Natl. Acad. Sci. U.S.A. 81:4622-4626(1984) [PubMed: 6379647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence and transcription of the region upstream of the E. coli gyrB gene." Adachi T., Mizuuchi K., Menzel R., Gellert M. Nucleic Acids Res. 12:6389-6395(1984) [PubMed: 6089112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Purification and preliminary characterization of the Escherichia coli K-12 recF protein." Griffin T.J. IV, Kolodner R.D. J. Bacteriol. 172:6291-6299(1990) [PubMed: 2228960] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13, DNA-BINDING.
[7]	"Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop." Sandler S.J., Chackerian B., Li J.T., Clark A.J. Nucleic Acids Res. 20:839-845(1992) [PubMed: 1542576] [Abstract] Cited for: MUTAGENESIS OF LYS-36.

Cross-references

Sequence databases
EMBLGenBankDDBJ	K02179 Genomic DNA. Translation: AAA24511.1. X04341 Genomic DNA. Translation: CAA27870.1. L10328 Genomic DNA. Translation: AAA62051.1. U00096 Genomic DNA. Translation: AAC76723.1. AP009048 Genomic DNA. Translation: BAE77594.1.
PIR	RQECF. A03547.
RefSeq	AP_004093.1. NP_418155.1.
3D structure databases
HSSP	HSSP built from PDB template 1F2T based on UniProtKB P58301.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A7H0. 12 interactions.
STRING	P0A7H0.
Proteomic databases
PRIDE	P0A7H0.
Genome annotation databases
GeneID	948209.
GenomeReviews	Gene locus JW3677 in contig AP009048_GR. Gene locus b3700 in contig U00096_GR.
KEGG	ecj:JW3677. eco:b3700.
Organism-specific databases
EchoBASE	EB0821.
EcoGene	EG10828. recF.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A7H0.
OMA	GQQRTLV.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10828-MON. MetaCyc:EG10828-MON.
Gene expression databases
Genevestigator	P0A7H0.
Family and domain databases
HAMAP	MF_00365. [Tree]
InterPro	IPR001238. DNA-binding_RecF. IPR018078. DNA-binding_RecF_CS. IPR003395. RecF/RecN/SMC_N. [Graphical view]
Pfam	PF02463. SMC_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00611. recf. 1 hit.
PROSITE	PS00617. RECF_1. 1 hit. PS00618. RECF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECF_ECOLI

Accession

Primary (citable) accession number: P0A7H0
Secondary accession number(s): P03016, Q2M812

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)