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Protein

Protein RecA

Gene

recA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).4 Publications

Enzyme regulationi

The rate of DNA-strand exchange is stimulated by RadA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 74ATPUniRule annotation1 Publication8

GO - Molecular functioni

GO - Biological processi

  • cell motility Source: EcoliWiki
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA recombinase assembly Source: GO_Central
  • DNA recombination Source: CACAO
  • mitotic recombination Source: GO_Central
  • response to ionizing radiation Source: EcoCyc
  • SOS response Source: CACAO
  • strand invasion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response, Stress response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10823-MONOMER.
ECOL316407:JW2669-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecAUniRule annotation
Alternative name(s):
Recombinase AUniRule annotation
Gene namesi
Name:recAUniRule annotation
Synonyms:lexB, recH, rnmB, tif, umuB, zab
Ordered Locus Names:b2699, JW2669
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10823. recA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Triple mazE-mazF-recA mutant cells no longer undergo an apoptotic-like death upon DNA damage characterized by membrane depolarization (PubMed:22412352).

Chemistry databases

ChEMBLiCHEMBL3434.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001227032 – 353Protein RecAAdd BLAST352

Proteomic databases

EPDiP0A7G6.
PaxDbiP0A7G6.
PRIDEiP0A7G6.

2D gel databases

SWISS-2DPAGEP0A7G6.

Expressioni

Inductioni

In response to low temperature. Sensitive to temperature through changes in the linking number of the DNA. 5.1-fold induced by hydroxyurea treatment (at protein level) (PubMed:20005847). mRNA levels are repressed in a mazE-mazF-mediated manner (PubMed:22412352).2 Publications

Interactioni

Subunit structurei

Polymerizes non-specifically on ssDNA to form filaments; filament formation requires ATP or ATP-gamma-S. Interacts with and activates LexA leading to autocatalytic cleavage of LexA, which derepresses the SOS regulon and activates DNA repair. Interacts with the C-terminus of RecB, facilitating loading of RecA onto ssDNA at chi sites. Interaction is decreased by ATP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mutLP233673EBI-370331,EBI-554913

Protein-protein interaction databases

BioGridi4259218. 403 interactors.
DIPiDIP-31832N.
IntActiP0A7G6. 28 interactors.
MINTiMINT-1300726.
STRINGi511145.b2699.

Chemistry databases

BindingDBiP0A7G6.

Structurei

Secondary structure

1353
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 22Combined sources15
Helixi24 – 26Combined sources3
Beta strandi27 – 29Combined sources3
Helixi30 – 32Combined sources3
Helixi34 – 36Combined sources3
Helixi46 – 51Combined sources6
Beta strandi53 – 58Combined sources6
Beta strandi61 – 66Combined sources6
Helixi73 – 86Combined sources14
Beta strandi91 – 97Combined sources7
Helixi102 – 107Combined sources6
Helixi112 – 114Combined sources3
Beta strandi116 – 118Combined sources3
Helixi123 – 136Combined sources14
Beta strandi140 – 145Combined sources6
Helixi147 – 149Combined sources3
Helixi153 – 156Combined sources4
Helixi167 – 186Combined sources20
Beta strandi189 – 194Combined sources6
Beta strandi196 – 198Combined sources3
Beta strandi208 – 212Combined sources5
Helixi214 – 219Combined sources6
Beta strandi221 – 253Combined sources33
Beta strandi258 – 264Combined sources7
Turni265 – 267Combined sources3
Helixi271 – 281Combined sources11
Beta strandi284 – 286Combined sources3
Beta strandi292 – 294Combined sources3
Beta strandi297 – 302Combined sources6
Helixi303 – 312Combined sources10
Helixi314 – 328Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AA3NMR-A269-331[»]
1N03electron microscopy20.00A/B/C/D/E/F/G2-353[»]
1REAX-ray2.70A2-353[»]
1U94X-ray1.90A2-353[»]
1U98X-ray2.00A2-353[»]
1U99X-ray2.60A2-353[»]
1XMSX-ray2.10A2-353[»]
1XMVX-ray1.90A2-353[»]
2REBX-ray2.30A2-353[»]
2RECelectron microscopy-A/B/C/D/E/F1-353[»]
3CMTX-ray3.15A/D2-335[»]
3CMUX-ray4.20A2-335[»]
3CMVX-ray4.30A/B/C/D/E/F/G/H2-335[»]
3CMWX-ray2.80A/C2-335[»]
3CMXX-ray3.40A/D2-335[»]
4TWZX-ray2.80A2-353[»]
ProteinModelPortaliP0A7G6.
SMRiP0A7G6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7G6.

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C68. Bacteria.
COG0468. LUCA.
HOGENOMiHOG000264120.
InParanoidiP0A7G6.
KOiK03553.
OMAiPPFKEAH.
PhylomeDBiP0A7G6.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
TIGRFAMsiTIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7G6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDENKQKA LAAALGQIEK QFGKGSIMRL GEDRSMDVET ISTGSLSLDI
60 70 80 90 100
ALGAGGLPMG RIVEIYGPES SGKTTLTLQV IAAAQREGKT CAFIDAEHAL
110 120 130 140 150
DPIYARKLGV DIDNLLCSQP DTGEQALEIC DALARSGAVD VIVVDSVAAL
160 170 180 190 200
TPKAEIEGEI GDSHMGLAAR MMSQAMRKLA GNLKQSNTLL IFINQIRMKI
210 220 230 240 250
GVMFGNPETT TGGNALKFYA SVRLDIRRIG AVKEGENVVG SETRVKVVKN
260 270 280 290 300
KIAAPFKQAE FQILYGEGIN FYGELVDLGV KEKLIEKAGA WYSYKGEKIG
310 320 330 340 350
QGKANATAWL KDNPETAKEI EKKVRELLLS NPNSTPDFSV DDSEGVAETN

EDF
Length:353
Mass (Da):37,973
Last modified:January 23, 2007 - v2
Checksum:i866EF8F83BE32A36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191Missing AA sequence (PubMed:7588783).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00328 Genomic DNA. Translation: CAA23618.1.
U00096 Genomic DNA. Translation: AAC75741.1.
AP009048 Genomic DNA. Translation: BAA16561.2.
PIRiG65049. RQECA.
RefSeqiNP_417179.1. NC_000913.3.
WP_000963143.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75741; AAC75741; b2699.
BAA16561; BAA16561; BAA16561.
GeneIDi947170.
KEGGiecj:JW2669.
eco:b2699.
PATRICi32120796. VBIEscCol129921_2790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00328 Genomic DNA. Translation: CAA23618.1.
U00096 Genomic DNA. Translation: AAC75741.1.
AP009048 Genomic DNA. Translation: BAA16561.2.
PIRiG65049. RQECA.
RefSeqiNP_417179.1. NC_000913.3.
WP_000963143.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AA3NMR-A269-331[»]
1N03electron microscopy20.00A/B/C/D/E/F/G2-353[»]
1REAX-ray2.70A2-353[»]
1U94X-ray1.90A2-353[»]
1U98X-ray2.00A2-353[»]
1U99X-ray2.60A2-353[»]
1XMSX-ray2.10A2-353[»]
1XMVX-ray1.90A2-353[»]
2REBX-ray2.30A2-353[»]
2RECelectron microscopy-A/B/C/D/E/F1-353[»]
3CMTX-ray3.15A/D2-335[»]
3CMUX-ray4.20A2-335[»]
3CMVX-ray4.30A/B/C/D/E/F/G/H2-335[»]
3CMWX-ray2.80A/C2-335[»]
3CMXX-ray3.40A/D2-335[»]
4TWZX-ray2.80A2-353[»]
ProteinModelPortaliP0A7G6.
SMRiP0A7G6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259218. 403 interactors.
DIPiDIP-31832N.
IntActiP0A7G6. 28 interactors.
MINTiMINT-1300726.
STRINGi511145.b2699.

Chemistry databases

BindingDBiP0A7G6.
ChEMBLiCHEMBL3434.

2D gel databases

SWISS-2DPAGEP0A7G6.

Proteomic databases

EPDiP0A7G6.
PaxDbiP0A7G6.
PRIDEiP0A7G6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75741; AAC75741; b2699.
BAA16561; BAA16561; BAA16561.
GeneIDi947170.
KEGGiecj:JW2669.
eco:b2699.
PATRICi32120796. VBIEscCol129921_2790.

Organism-specific databases

EchoBASEiEB0816.
EcoGeneiEG10823. recA.

Phylogenomic databases

eggNOGiENOG4105C68. Bacteria.
COG0468. LUCA.
HOGENOMiHOG000264120.
InParanoidiP0A7G6.
KOiK03553.
OMAiPPFKEAH.
PhylomeDBiP0A7G6.

Enzyme and pathway databases

BioCyciEcoCyc:EG10823-MONOMER.
ECOL316407:JW2669-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A7G6.
PROiP0A7G6.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
TIGRFAMsiTIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECA_ECOLI
AccessioniPrimary (citable) accession number: P0A7G6
Secondary accession number(s): P03017, P26347, P78213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.