ID   RBFA_ECOLI              Reviewed;         133 AA.
AC   P0A7G2; P09170; Q2M943;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=Ribosome-binding factor A;
DE   AltName: Full=Protein P15B;
GN   Name=rbfA; Synonyms=P15B, yhbB; OrderedLocusNames=b3167, JW3136;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   MEDLINE=89083498; PubMed=2849753; DOI=10.1093/nar/16.22.10803;
RA   Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M.,
RA   Hershey J.W.B.;
RT   "The existence of two genes between infB and rpsO in the Escherichia
RT   coli genome: DNA sequencing and S1 nuclease mapping.";
RL   Nucleic Acids Res. 16:10803-10816(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   PARTIAL PROTEIN SEQUENCE OF 2-12.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Frutiger S., Hughes G.J., Tonella L., Hochstrasser D.F.;
RL   Submitted (SEP-1996) to UniProtKB.
RN   [5]
RP   IDENTIFICATION.
RX   MEDLINE=95212911; PubMed=7535280;
RA   Dammel C.S., Noller H.F.;
RT   "Suppression of a cold-sensitive mutation in 16S rRNA by
RT   overexpression of a novel ribosome-binding factor, RbfA.";
RL   Genes Dev. 9:626-637(1995).
RN   [6]
RP   FUNCTION.
RC   STRAIN=MW100;
RX   MEDLINE=98083058; PubMed=9422595;
RA   Bylund G.O., Wipemo L.C., Lundberg L.A., Wikstroem P.M.;
RT   "RimM and RbfA are essential for efficient processing of 16S rRNA in
RT   Escherichia coli.";
RL   J. Bacteriol. 180:73-82(1998).
CC   -!- FUNCTION: Essential for efficient processing of 16S rRNA. Probably
CC       part of the 30S subunit prior to or during the final step in the
CC       processing of 16S free 30S ribosomal subunits. Could be some
CC       accessory protein needed for efficient assembly of the 30S
CC       subunit. May interact with the 5'-terminal helix region of 16S
CC       rRNA. Has affinity for free ribosomal 30S subunits but not for 70S
CC       ribosomes. Overexpression suppresses rimM deletion mutants.
CC   -!- INTERACTION:
CC       P18843:nadE; NbExp=1; IntAct=EBI-1131774, EBI-548960;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- SIMILARITY: Belongs to the rbfA family.
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DR   EMBL; X13270; CAA31634.1; -; Genomic_DNA.
DR   EMBL; X13775; CAA32020.1; -; Genomic_DNA.
DR   EMBL; U18997; AAA57970.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76201.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77213.1; -; Genomic_DNA.
DR   PIR; S01915; Q9EC15.
DR   RefSeq; AP_003712.1; -.
DR   RefSeq; NP_417636.1; -.
DR   PDB; 1KKG; NMR; -; A=1-108.
DR   PDBsum; 1KKG; -.
DR   IntAct; P0A7G2; 2.
DR   SWISS-2DPAGE; P0A7G2; -.
DR   GeneID; 947685; -.
DR   GenomeReviews; AP009048_GR; JW3136.
DR   GenomeReviews; U00096_GR; b3167.
DR   KEGG; ecj:JW3136; -.
DR   KEGG; eco:b3167; -.
DR   EchoBASE; EB1165; -.
DR   EcoGene; EG11178; rbfA.
DR   HOGENOM; P0A7G2; -.
DR   OMA; P0A7G2; SIEYGNR.
DR   BioCyc; EcoCyc:EG11178-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0006364; P:rRNA processing; IEA:HAMAP.
DR   HAMAP; MF_00003; -; 1.
DR   InterPro; IPR000238; Ribosome-bd_factorA.
DR   Pfam; PF02033; RBFA; 1.
DR   ProDom; PD007327; Rib_bind_factA; 1.
DR   TIGRFAMs; TIGR00082; rbfA; 1.
DR   PROSITE; PS01319; RBFA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing;
KW   rRNA processing.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    133       Ribosome-binding factor A.
FT                                /FTId=PRO_0000102658.
FT   STRAND        5      7
FT   HELIX         8     22
FT   STRAND       25     29
FT   HELIX        30     32
FT   STRAND       41     44
FT   TURN         45     48
FT   STRAND       49     55
FT   HELIX        58     60
FT   HELIX        62     74
FT   HELIX        76     86
FT   STRAND       94     99
FT   STRAND      104    106
SQ   SEQUENCE   133 AA;  15154 MW;  C8EE4FBFD8C01F08 CRC64;
     MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK
     DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMRMS NLVTSVVKHD
     EERRVNPDDS KED
//