P0A7G2 (RBFA_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 63.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ribosome-binding factor A Alternative name(s): Protein P15B | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 133 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Overexpression suppresses a cold-sensitive C23U 16S rRNA mutation and rimM deletion mutants. Its function probably overlaps Era. Ref.6 Ref.8 |
| Subcellular location | Cytoplasm Potential HAMAP MF_00003. |
| Disruption phenotype | Unable to grow at 25 degrees Celsius; has decreased amounts of 70S ribosomes and increased amounts of 30s and 50S subunits. Both phenotypes are partially suppressed by overexpression of Era and completely suppressed by overexpression of Era-delta 'Ala-40-Gly-49' mutation. Ref.7 |
| Sequence similarities | Belongs to the RbfA family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Ribosome biogenesis rRNA processing |
| Cellular component | Cytoplasm |
| Technical term | 3D-structure Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | maturation of SSU-rRNA Inferred from mutant phenotype Ref.6. Source: EcoliWiki |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| nadE | P18843 | 1 | EBI-1131774,EBI-548960 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.4 | ||||||||||||||||||||||||||
| Chain | 2 – 133 | 132 | Ribosome-binding factor A HAMAP MF_00003 | PRO_0000102658 | |||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||
| Beta strand | 5 – 7 | 3 | |||||||||||||||||||||||||||
| Helix | 8 – 22 | 15 | |||||||||||||||||||||||||||
| Beta strand | 25 – 29 | 5 | |||||||||||||||||||||||||||
| Helix | 30 – 32 | 3 | |||||||||||||||||||||||||||
| Beta strand | 41 – 44 | 4 | |||||||||||||||||||||||||||
| Turn | 45 – 48 | 4 | |||||||||||||||||||||||||||
| Beta strand | 49 – 55 | 7 | |||||||||||||||||||||||||||
| Helix | 58 – 60 | 3 | |||||||||||||||||||||||||||
| Helix | 62 – 74 | 13 | |||||||||||||||||||||||||||
| Helix | 76 – 86 | 11 | |||||||||||||||||||||||||||
| Beta strand | 94 – 99 | 6 | |||||||||||||||||||||||||||
| Beta strand | 104 – 106 | 3 | |||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping." Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M., Hershey J.W.B. Nucleic Acids Res. 16:10803-10816(1988) [PubMed: 2849753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | Frutiger S., Hughes G.J., Tonella L., Hochstrasser D.F. Submitted (SEP-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA." Dammel C.S., Noller H.F. Genes Dev. 9:626-637(1995) [PubMed: 7535280] [Abstract] Cited for: IDENTIFICATION, SUPPRESSION OF AN RRNA COLD-SENSITIVE MUTATION. |
| [6] | "RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli." Bylund G.O., Wipemo L.C., Lundberg L.A., Wikstroem P.M. J. Bacteriol. 180:73-82(1998) [PubMed: 9422595] [Abstract] Cited for: FUNCTION. Strain: MW100. |
| [7] | "Suppression of defective ribosome assembly in a rbfA deletion mutant by overexpression of Era, an essential GTPase in Escherichia coli." Inoue K., Alsina J., Chen J., Inouye M. Mol. Microbiol. 48:1005-1016(2003) [PubMed: 12753192] [Abstract] Cited for: DISRUPTION PHENOTYPE, SUPPRESSION BY ERA. Strain: K12 / ATCC 35607 / JM83 and K12 / MC4100. |
| [8] | "Era and RbfA have overlapping function in ribosome biogenesis in Escherichia coli." Inoue K., Chen J., Tan Q., Inouye M. J. Mol. Microbiol. Biotechnol. 11:41-52(2006) [PubMed: 16825789] [Abstract] Cited for: FUNCTION. Strain: K12 / ATCC 35607 / JM83 and K12 / MC4100. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | X13270 Genomic DNA. Translation: CAA31634.1. X13775 Genomic DNA. Translation: CAA32020.1. U18997 Genomic DNA. Translation: AAA57970.1. U00096 Genomic DNA. Translation: AAC76201.1. AP009048 Genomic DNA. Translation: BAE77213.1. | ||||||||||||
| PIR | Q9EC15. S01915. | ||||||||||||
| RefSeq | NP_417636.1. NC_000913.2. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||
| ProteinModelPortal | P0A7G2. | ||||||||||||
| SMR | P0A7G2. Positions 1-108. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P0A7G2. 2 interactions. | ||||||||||||
| MINT | MINT-1265558. | ||||||||||||
2D gel databases | |||||||||||||
| SWISS-2DPAGE | P0A7G2. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| EnsemblBacteria | EBESCT00000002223; EBESCP00000002223; EBESCG00000001823. EBESCT00000002224; EBESCP00000002224; EBESCG00000001823. EBESCT00000017462; EBESCP00000016753; EBESCG00000016518. | ||||||||||||
| GeneID | 947685. | ||||||||||||
| GenomeReviews | Gene locus JW3136 in contig AP009048_GR. Gene locus b3167 in contig U00096_GR. | ||||||||||||
| KEGG | ecj:JW3136. eco:b3167. | ||||||||||||
| PATRIC | 32121752. VBIEscCol129921_3262. | ||||||||||||
Organism-specific databases | |||||||||||||
| EchoBASE | EB1165. | ||||||||||||
| EcoGene | EG11178. rbfA. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | COG0858. | ||||||||||||
| GeneTree | EBGT00050000011345. | ||||||||||||
| HOGENOM | HBG751055. | ||||||||||||
| OMA | QDASGFI. | ||||||||||||
| PhylomeDB | P0A7G2. | ||||||||||||
| ProtClustDB | PRK00521. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BioCyc | EcoCyc:EG11178-MONOMER. | ||||||||||||
Gene expression databases | |||||||||||||
| Genevestigator | P0A7G2. | ||||||||||||
Family and domain databases | |||||||||||||
| HAMAP | MF_00003. RbfA. [Tree] | ||||||||||||
| InterPro | IPR015946. KH_dom-like_a/b. IPR000238. Ribosome-bd_facA. IPR023799. Ribosome-bd_facA_dom. IPR020053. Ribosome-bd_factorA_CS. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.30.300.20. KH_prok. 1 hit. | ||||||||||||
| KO | K02834. | ||||||||||||
| Pfam | PF02033. RBFA. 1 hit. [Graphical view] | ||||||||||||
| ProDom | PD007327. Rib_bd_factA. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| SUPFAM | SSF89919. Rib_bd_factA. 1 hit. | ||||||||||||
| TIGRFAMs | TIGR00082. RbfA. 1 hit. | ||||||||||||
| PROSITE | PS01319. RBFA. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | RBFA_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0A7G2 Secondary accession number(s): P09170, Q2M943 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

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