P0A7G2 (RBFA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome-binding factor A

Alternative name(s):
Protein P15B

Gene names

Name:	rbfA
Synonyms:	P15B, yhbB
Ordered Locus Names:	b3167, JW3136

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Overexpression suppresses a cold-sensitive C23U 16S rRNA mutation and rimM deletion mutants. Its function probably overlaps Era. Ref.6 Ref.8
Subcellular location	Cytoplasm Potential HAMAP MF_00003.
Disruption phenotype	Unable to grow at 25 degrees Celsius; has decreased amounts of 70S ribosomes and increased amounts of 30s and 50S subunits. Both phenotypes are partially suppressed by overexpression of Era and completely suppressed by overexpression of Era-delta 'Ala-40-Gly-49' mutation. Ref.7
Sequence similarities	Belongs to the RbfA family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA Inferred from mutant phenotype Ref.6. Source: EcoliWiki
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1131774,EBI-548960

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4

Chain

2 – 133

132

Ribosome-binding factor A HAMAP MF_00003

PRO_0000102658

Secondary structure

1

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133

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Helix

8 – 22

15

Beta strand

25 – 29

5

Helix

30 – 32

3

Beta strand

41 – 44

4

Turn

45 – 48

4

Beta strand

49 – 55

7

Helix

58 – 60

3

Helix

62 – 74

13

Helix

76 – 86

11

Beta strand

94 – 99

6

Beta strand

104 – 106

3

Sequences

	Sequence		Length	Mass (Da)
	P0A7G2 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C8EE4FBFD8C01F08	FASTA	133	15,154
	10 20 30 40 50 60 MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK 70 80 90 100 110 120 DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMRMS NLVTSVVKHD 130 EERRVNPDDS KED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping." Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M., Hershey J.W.B. Nucleic Acids Res. 16:10803-10816(1988) [PubMed: 2849753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Frutiger S., Hughes G.J., Tonella L., Hochstrasser D.F. Submitted (SEP-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA." Dammel C.S., Noller H.F. Genes Dev. 9:626-637(1995) [PubMed: 7535280] [Abstract] Cited for: IDENTIFICATION, SUPPRESSION OF AN RRNA COLD-SENSITIVE MUTATION.
[6]	"RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli." Bylund G.O., Wipemo L.C., Lundberg L.A., Wikstroem P.M. J. Bacteriol. 180:73-82(1998) [PubMed: 9422595] [Abstract] Cited for: FUNCTION. Strain: MW100.
[7]	"Suppression of defective ribosome assembly in a rbfA deletion mutant by overexpression of Era, an essential GTPase in Escherichia coli." Inoue K., Alsina J., Chen J., Inouye M. Mol. Microbiol. 48:1005-1016(2003) [PubMed: 12753192] [Abstract] Cited for: DISRUPTION PHENOTYPE, SUPPRESSION BY ERA. Strain: K12 / ATCC 35607 / JM83 and K12 / MC4100.
[8]	"Era and RbfA have overlapping function in ribosome biogenesis in Escherichia coli." Inoue K., Chen J., Tan Q., Inouye M. J. Mol. Microbiol. Biotechnol. 11:41-52(2006) [PubMed: 16825789] [Abstract] Cited for: FUNCTION. Strain: K12 / ATCC 35607 / JM83 and K12 / MC4100.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X13270 Genomic DNA. Translation: CAA31634.1.
X13775 Genomic DNA. Translation: CAA32020.1.
U18997 Genomic DNA. Translation: AAA57970.1.
U00096 Genomic DNA. Translation: AAC76201.1.
AP009048 Genomic DNA. Translation: BAE77213.1.

PIR

Q9EC15. S01915.

RefSeq

NP_417636.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KKG	NMR	-	A	1-108	[»]

ProteinModelPortal

P0A7G2.

SMR

P0A7G2. Positions 1-108.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7G2. 2 interactions.

MINT

MINT-1265558.

2D gel databases

SWISS-2DPAGE

P0A7G2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002223; EBESCP00000002223; EBESCG00000001823.
EBESCT00000002224; EBESCP00000002224; EBESCG00000001823.
EBESCT00000017462; EBESCP00000016753; EBESCG00000016518.

GeneID

947685.

GenomeReviews

Gene locus JW3136 in contig AP009048_GR.
Gene locus b3167 in contig U00096_GR.

KEGG

ecj:JW3136.
eco:b3167.

PATRIC

32121752. VBIEscCol129921_3262.

Organism-specific databases

EchoBASE

EB1165.

EcoGene

EG11178. rbfA.

Phylogenomic databases

eggNOG

COG0858.

GeneTree

EBGT00050000011345.

HOGENOM

HBG751055.

OMA

QDASGFI.

PhylomeDB

P0A7G2.

ProtClustDB

PRK00521.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11178-MONOMER.

Gene expression databases

Genevestigator

P0A7G2.

Family and domain databases

HAMAP

MF_00003. RbfA.
[Tree]

InterPro

IPR015946. KH_dom-like_a/b.
IPR000238. Ribosome-bd_facA.
IPR023799. Ribosome-bd_facA_dom.
IPR020053. Ribosome-bd_factorA_CS.
[Graphical view]

Gene3D

G3DSA:3.30.300.20. KH_prok. 1 hit.

KO

K02834.

Pfam

PF02033. RBFA. 1 hit.
[Graphical view]

ProDom

PD007327. Rib_bd_factA. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF89919. Rib_bd_factA. 1 hit.

TIGRFAMs

TIGR00082. RbfA. 1 hit.

PROSITE

PS01319. RBFA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RBFA_ECOLI

Accession

Primary (citable) accession number: P0A7G2
Secondary accession number(s): P09170, Q2M943

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program