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Protein

Ribosome-binding factor A

Gene

rbfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Overexpression suppresses a cold-sensitive C23U 16S rRNA mutation and rimM deletion mutants. Its function probably overlaps Era.2 Publications

GO - Molecular functioni

  • ribosomal small subunit binding Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • maturation of SSU-rRNA Source: EcoliWiki
  • response to cold Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG11178-MONOMER.
ECOL316407:JW3136-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-binding factor AUniRule annotation
Alternative name(s):
Protein P15B
Gene namesi
Name:rbfAUniRule annotation
Synonyms:P15B, yhbB
Ordered Locus Names:b3167, JW3136
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11178. rbfA.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Unable to grow at 25 degrees Celsius; has decreased amounts of 70S ribosomes and increased amounts of 30s and 50S subunits. Both phenotypes are partially suppressed by overexpression of Era and completely suppressed by overexpression of Era-delta 'Ala-40-Gly-49' mutation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 133132Ribosome-binding factor APRO_0000102658Add
BLAST

Proteomic databases

EPDiP0A7G2.
PaxDbiP0A7G2.
PRIDEiP0A7G2.

2D gel databases

SWISS-2DPAGEP0A7G2.

Interactioni

Protein-protein interaction databases

BioGridi4262435. 56 interactions.
IntActiP0A7G2. 2 interactions.
MINTiMINT-1265558.
STRINGi511145.b3167.

Structurei

Secondary structure

1
133
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 73Combined sources
Helixi8 – 2215Combined sources
Beta strandi25 – 295Combined sources
Helixi30 – 323Combined sources
Beta strandi41 – 444Combined sources
Turni45 – 484Combined sources
Beta strandi49 – 557Combined sources
Helixi58 – 603Combined sources
Helixi62 – 7413Combined sources
Helixi76 – 8611Combined sources
Beta strandi94 – 996Combined sources
Beta strandi104 – 1063Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KKGNMR-A1-108[»]
ProteinModelPortaliP0A7G2.
SMRiP0A7G2. Positions 1-108.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7G2.

Family & Domainsi

Sequence similaritiesi

Belongs to the RbfA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KG7. Bacteria.
COG0858. LUCA.
HOGENOMiHOG000218325.
InParanoidiP0A7G2.
KOiK02834.
OMAiVMPEIRF.
OrthoDBiEOG6XQ3TJ.
PhylomeDBiP0A7G2.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
HAMAPiMF_00003. RbfA.
InterProiIPR015946. KH_dom-like_a/b.
IPR000238. Ribosome-bd_facA.
IPR023799. Ribosome-bd_facA_dom.
IPR020053. Ribosome-bd_factorA_CS.
[Graphical view]
PfamiPF02033. RBFA. 1 hit.
[Graphical view]
ProDomiPD007327. Rib_bd_factA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF89919. SSF89919. 1 hit.
TIGRFAMsiTIGR00082. rbfA. 1 hit.
PROSITEiPS01319. RBFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA
60 70 80 90 100
KVYVTFLNDK DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD
110 120 130
NSLVEGMRMS NLVTSVVKHD EERRVNPDDS KED
Length:133
Mass (Da):15,154
Last modified:January 23, 2007 - v2
Checksum:iC8EE4FBFD8C01F08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13270 Genomic DNA. Translation: CAA31634.1.
X13775 Genomic DNA. Translation: CAA32020.1.
U18997 Genomic DNA. Translation: AAA57970.1.
U00096 Genomic DNA. Translation: AAC76201.1.
AP009048 Genomic DNA. Translation: BAE77213.1.
PIRiS01915. Q9EC15.
RefSeqiNP_417636.1. NC_000913.3.
WP_001040205.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76201; AAC76201; b3167.
BAE77213; BAE77213; BAE77213.
GeneIDi947685.
KEGGiecj:JW3136.
eco:b3167.
PATRICi32121752. VBIEscCol129921_3262.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13270 Genomic DNA. Translation: CAA31634.1.
X13775 Genomic DNA. Translation: CAA32020.1.
U18997 Genomic DNA. Translation: AAA57970.1.
U00096 Genomic DNA. Translation: AAC76201.1.
AP009048 Genomic DNA. Translation: BAE77213.1.
PIRiS01915. Q9EC15.
RefSeqiNP_417636.1. NC_000913.3.
WP_001040205.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KKGNMR-A1-108[»]
ProteinModelPortaliP0A7G2.
SMRiP0A7G2. Positions 1-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262435. 56 interactions.
IntActiP0A7G2. 2 interactions.
MINTiMINT-1265558.
STRINGi511145.b3167.

2D gel databases

SWISS-2DPAGEP0A7G2.

Proteomic databases

EPDiP0A7G2.
PaxDbiP0A7G2.
PRIDEiP0A7G2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76201; AAC76201; b3167.
BAE77213; BAE77213; BAE77213.
GeneIDi947685.
KEGGiecj:JW3136.
eco:b3167.
PATRICi32121752. VBIEscCol129921_3262.

Organism-specific databases

EchoBASEiEB1165.
EcoGeneiEG11178. rbfA.

Phylogenomic databases

eggNOGiENOG4105KG7. Bacteria.
COG0858. LUCA.
HOGENOMiHOG000218325.
InParanoidiP0A7G2.
KOiK02834.
OMAiVMPEIRF.
OrthoDBiEOG6XQ3TJ.
PhylomeDBiP0A7G2.

Enzyme and pathway databases

BioCyciEcoCyc:EG11178-MONOMER.
ECOL316407:JW3136-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A7G2.
PROiP0A7G2.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
HAMAPiMF_00003. RbfA.
InterProiIPR015946. KH_dom-like_a/b.
IPR000238. Ribosome-bd_facA.
IPR023799. Ribosome-bd_facA_dom.
IPR020053. Ribosome-bd_factorA_CS.
[Graphical view]
PfamiPF02033. RBFA. 1 hit.
[Graphical view]
ProDomiPD007327. Rib_bd_factA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF89919. SSF89919. 1 hit.
TIGRFAMsiTIGR00082. rbfA. 1 hit.
PROSITEiPS01319. RBFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping."
    Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M., Hershey J.W.B.
    Nucleic Acids Res. 16:10803-10816(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Frutiger S., Hughes G.J., Tonella L., Hochstrasser D.F.
    Submitted (SEP-1996) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-12.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA."
    Dammel C.S., Noller H.F.
    Genes Dev. 9:626-637(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUPPRESSION OF AN RRNA COLD-SENSITIVE MUTATION.
  6. "RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli."
    Bylund G.O., Wipemo L.C., Lundberg L.A., Wikstroem P.M.
    J. Bacteriol. 180:73-82(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: MW100.
  7. "Suppression of defective ribosome assembly in a rbfA deletion mutant by overexpression of Era, an essential GTPase in Escherichia coli."
    Inoue K., Alsina J., Chen J., Inouye M.
    Mol. Microbiol. 48:1005-1016(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, SUPPRESSION BY ERA.
    Strain: K12 / ATCC 35607 / JM83 and K12 / MC4100.
  8. "Era and RbfA have overlapping function in ribosome biogenesis in Escherichia coli."
    Inoue K., Chen J., Tan Q., Inouye M.
    J. Mol. Microbiol. Biotechnol. 11:41-52(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / ATCC 35607 / JM83 and K12 / MC4100.

Entry informationi

Entry nameiRBFA_ECOLI
AccessioniPrimary (citable) accession number: P0A7G2
Secondary accession number(s): P09170, Q2M943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.