Reviewed, UniProtKB/Swiss-Prot P0A7G2 (RBFA_ECOLI)

Names and origin

Protein names

Recommended name:
    Ribosome-binding factor A
Alternative name(s):
    Protein P15B

Gene names

Name:	rbfA
Synonyms:	P15B, yhbB
Ordered Locus Names:	b3167, JW3136

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Overexpression suppresses rimM deletion mutants. Ref.6
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the rbfA family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1131774,EBI-548960

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4

Chain

2 – 133

132

Ribosome-binding factor A HAMAP MF_00003

PRO_0000102658

Secondary structure

1

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133

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Helix

8 – 22

15

Beta strand

25 – 29

5

Helix

30 – 32

3

Beta strand

41 – 44

4

Turn

45 – 48

4

Beta strand

49 – 55

7

Helix

58 – 60

3

Helix

62 – 74

13

Helix

76 – 86

11

Beta strand

94 – 99

6

Beta strand

104 – 106

3

Sequences

	Sequence		Length	Mass (Da)
	P0A7G2-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C8EE4FBFD8C01F08	FASTA	133	15,154
	10 20 30 40 50 60 MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK 70 80 90 100 110 120 DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMRMS NLVTSVVKHD 130 EERRVNPDDS KED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping." Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M., Hershey J.W.B. Nucleic Acids Res. 16:10803-10816(1988) [PubMed: 2849753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Frutiger S., Hughes G.J., Tonella L., Hochstrasser D.F. Submitted (SEP-1996) to UniProtKB Cited for: PARTIAL PROTEIN SEQUENCE OF 2-12. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA." Dammel C.S., Noller H.F. Genes Dev. 9:626-637(1995) [PubMed: 7535280] [Abstract] Cited for: IDENTIFICATION.
[6]	"RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli." Bylund G.O., Wipemo L.C., Lundberg L.A., Wikstroem P.M. J. Bacteriol. 180:73-82(1998) [PubMed: 9422595] [Abstract] Cited for: FUNCTION. Strain: MW100.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X13270 Genomic DNA. Translation: CAA31634.1.
X13775 Genomic DNA. Translation: CAA32020.1.
U18997 Genomic DNA. Translation: AAA57970.1.
U00096 Genomic DNA. Translation: AAC76201.1.
AP009048 Genomic DNA. Translation: BAE77213.1.

PIR

Q9EC15. S01915.

RefSeq

AP_003712.1.
NP_417636.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KKG	NMR	-	A	1-108	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7G2. 2 interactions.

2-D gel databases

SWISS-2DPAGE

P0A7G2.

Genome annotation databases

GeneID

947685.

GenomeReviews

Gene locus JW3136 in contig AP009048_GR.
Gene locus b3167 in contig U00096_GR.

KEGG

ecj:JW3136.
eco:b3167.

Organism-specific databases

EchoBASE

EB1165.

EcoGene

EG11178. rbfA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7G2.

OMA

P0A7G2. SIEYGNR.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11178-MON.

Family and domain databases

HAMAP

MF_00003.
[Tree]

InterPro

IPR000238. Ribosome-bd_factorA.
[Graphical view]

Pfam

PF02033. RBFA. 1 hit.
[Graphical view]

ProDom

PD007327. Rib_bind_factA. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00082. rbfA. 1 hit.

PROSITE

PS01319. RBFA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RBFA_ECOLI

Accession

Primary (citable) accession number: P0A7G2
Secondary accession number(s): P09170, Q2M943

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)