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Protein

Aspartate carbamoyltransferase regulatory chain

Gene

pyrI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in allosteric regulation of aspartate carbamoyltransferase.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Zinc1
Metal bindingi114Zinc1
Metal bindingi138Zinc1
Metal bindingi141Zinc1

GO - Molecular functioni

  • zinc ion binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ASPCARBREG-MONOMER.
ECOL316407:JW4203-MONOMER.
MetaCyc:ASPCARBREG-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase regulatory chain
Gene namesi
Name:pyrI
Ordered Locus Names:b4244, JW4203
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10811. pyrI.

Subcellular locationi

GO - Cellular componenti

  • aspartate carbamoyltransferase complex Source: InterPro
  • cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001423032 – 153Aspartate carbamoyltransferase regulatory chainAdd BLAST152

Proteomic databases

EPDiP0A7F3.
PaxDbiP0A7F3.
PRIDEiP0A7F3.

2D gel databases

SWISS-2DPAGEP0A7F3.

Interactioni

Subunit structurei

Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
pyrBP0A78614EBI-906630,EBI-906620

Protein-protein interaction databases

BioGridi4263117. 2 interactors.
DIPiDIP-35088N.
IntActiP0A7F3. 2 interactors.
MINTiMINT-1541364.
STRINGi511145.b4244.

Structurei

Secondary structure

1153
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Beta strandi13 – 21Combined sources9
Turni23 – 25Combined sources3
Helixi26 – 32Combined sources7
Turni33 – 36Combined sources4
Beta strandi37 – 40Combined sources4
Beta strandi42 – 50Combined sources9
Turni51 – 53Combined sources3
Beta strandi54 – 62Combined sources9
Helixi68 – 72Combined sources5
Turni73 – 77Combined sources5
Beta strandi78 – 80Combined sources3
Beta strandi82 – 87Combined sources6
Beta strandi90 – 95Combined sources6
Beta strandi101 – 106Combined sources6
Beta strandi112 – 114Combined sources3
Turni115 – 118Combined sources4
Beta strandi119 – 121Combined sources3
Beta strandi124 – 129Combined sources6
Beta strandi131 – 138Combined sources8
Turni139 – 141Combined sources3
Beta strandi144 – 146Combined sources3
Helixi147 – 152Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ACMX-ray2.80B/D1-153[»]
1AT1X-ray2.80B/D1-153[»]
1D09X-ray2.10B/D1-153[»]
1EZZX-ray2.70B/D1-153[»]
1F1BX-ray2.30B/D1-153[»]
1I5OX-ray2.80B/D1-153[»]
1NBEX-ray2.60B/D1-153[»]
1Q95X-ray2.46G/H/I/J/K/L1-153[»]
1R0BX-ray2.90G/H/I/J/K/L1-153[»]
1R0CX-ray2.37B/H1-153[»]
1RAAX-ray2.50B/D1-153[»]
1RABX-ray2.50B/D1-153[»]
1RACX-ray2.50B/D1-153[»]
1RADX-ray2.50B/D1-153[»]
1RAEX-ray2.50B/D1-153[»]
1RAFX-ray2.50B/D1-153[»]
1RAGX-ray2.50B/D1-153[»]
1RAHX-ray2.50B/D1-153[»]
1RAIX-ray2.50B/D1-153[»]
1SKUX-ray2.60B/D1-153[»]
1TTHX-ray2.80B/D1-153[»]
1TU0X-ray2.55B/D1-153[»]
1TUGX-ray2.10B/D1-153[»]
1XJWX-ray2.71B/D1-153[»]
1ZA1X-ray2.20B/D1-153[»]
1ZA2X-ray2.50B/D1-153[»]
2A0FX-ray2.90B/D1-153[»]
2AIRX-ray2.00B/H1-153[»]
2AT1X-ray2.80B/D1-153[»]
2ATCX-ray3.00B1-153[»]
2FZCX-ray2.10B/D1-153[»]
2FZGX-ray2.25B/D1-153[»]
2FZKX-ray2.50B/D1-153[»]
2H3EX-ray2.30B/D1-153[»]
2HSEX-ray2.60B/D1-153[»]
2IPOX-ray2.60B/D1-153[»]
2QG9X-ray2.70B/D1-153[»]
2QGFX-ray2.20B/D1-153[»]
3AT1X-ray2.80B/D1-153[»]
3D7SX-ray2.80B/D1-153[»]
3MPUX-ray2.86B/D/F1-153[»]
4AT1X-ray2.60B/D1-153[»]
4E2FX-ray2.80B/D/F/H/J/L1-153[»]
4F04X-ray2.30B/D1-153[»]
4FYVX-ray2.10B/D1-153[»]
4FYWX-ray2.10B/D1-153[»]
4FYXX-ray2.09B/D1-153[»]
4FYYX-ray1.94B/D1-153[»]
4WTOX-ray2.03B/D1-153[»]
5AT1X-ray2.60B/D1-153[»]
6AT1X-ray2.60B/D1-153[»]
7AT1X-ray2.80B/D1-153[»]
8AT1X-ray2.80B/D1-153[»]
8ATCX-ray2.50B/D1-153[»]
9ATCX-ray2.40B8-153[»]
ProteinModelPortaliP0A7F3.
SMRiP0A7F3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7F3.

Family & Domainsi

Sequence similaritiesi

Belongs to the PyrI family.Curated

Phylogenomic databases

eggNOGiENOG4108R9M. Bacteria.
COG1781. LUCA.
HOGENOMiHOG000113530.
InParanoidiP0A7F3.
KOiK00610.
OMAiMNVPSDR.
PhylomeDBiP0A7F3.

Family and domain databases

Gene3Di2.30.30.20. 1 hit.
3.30.70.140. 1 hit.
HAMAPiMF_00002. Asp_carb_tr_reg. 1 hit.
InterProiIPR020545. Asp_carbamoyltransf_reg_N.
IPR002801. Asp_carbamoylTrfase_reg.
IPR020542. Asp_carbamoyltrfase_reg_C.
[Graphical view]
PfamiPF01948. PyrI. 1 hit.
PF02748. PyrI_C. 1 hit.
[Graphical view]
ProDomiPD006194. Asp_carbamoylTrfase_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54893. SSF54893. 1 hit.
SSF57825. SSF57825. 1 hit.
TIGRFAMsiTIGR00240. ATCase_reg. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHDNKLQVE AIKRGTVIDH IPAQIGFKLL SLFKLTETDQ RITIGLNLPS
60 70 80 90 100
GEMGRKDLIK IENTFLSEDQ VDQLALYAPQ ATVNRIDNYE VVGKSRPSLP
110 120 130 140 150
ERIDNVLVCP NSNCISHAEP VSSSFAVRKR ANDIALKCKY CEKEFSHNVV

LAN
Length:153
Mass (Da):17,121
Last modified:January 23, 2007 - v2
Checksum:i81A1631614E549EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01472 Genomic DNA. Translation: AAA24477.1.
U14003 Genomic DNA. Translation: AAA97141.1.
U00096 Genomic DNA. Translation: AAC77201.1.
AP009048 Genomic DNA. Translation: BAE78243.1.
M28578 Genomic DNA. Translation: AAA24487.1.
PIRiA93985. DTECR.
RefSeqiNP_418665.1. NC_000913.3.
WP_000148581.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77201; AAC77201; b4244.
BAE78243; BAE78243; BAE78243.
GeneIDi948763.
KEGGiecj:JW4203.
eco:b4244.
PATRICi32124065. VBIEscCol129921_4377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01472 Genomic DNA. Translation: AAA24477.1.
U14003 Genomic DNA. Translation: AAA97141.1.
U00096 Genomic DNA. Translation: AAC77201.1.
AP009048 Genomic DNA. Translation: BAE78243.1.
M28578 Genomic DNA. Translation: AAA24487.1.
PIRiA93985. DTECR.
RefSeqiNP_418665.1. NC_000913.3.
WP_000148581.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ACMX-ray2.80B/D1-153[»]
1AT1X-ray2.80B/D1-153[»]
1D09X-ray2.10B/D1-153[»]
1EZZX-ray2.70B/D1-153[»]
1F1BX-ray2.30B/D1-153[»]
1I5OX-ray2.80B/D1-153[»]
1NBEX-ray2.60B/D1-153[»]
1Q95X-ray2.46G/H/I/J/K/L1-153[»]
1R0BX-ray2.90G/H/I/J/K/L1-153[»]
1R0CX-ray2.37B/H1-153[»]
1RAAX-ray2.50B/D1-153[»]
1RABX-ray2.50B/D1-153[»]
1RACX-ray2.50B/D1-153[»]
1RADX-ray2.50B/D1-153[»]
1RAEX-ray2.50B/D1-153[»]
1RAFX-ray2.50B/D1-153[»]
1RAGX-ray2.50B/D1-153[»]
1RAHX-ray2.50B/D1-153[»]
1RAIX-ray2.50B/D1-153[»]
1SKUX-ray2.60B/D1-153[»]
1TTHX-ray2.80B/D1-153[»]
1TU0X-ray2.55B/D1-153[»]
1TUGX-ray2.10B/D1-153[»]
1XJWX-ray2.71B/D1-153[»]
1ZA1X-ray2.20B/D1-153[»]
1ZA2X-ray2.50B/D1-153[»]
2A0FX-ray2.90B/D1-153[»]
2AIRX-ray2.00B/H1-153[»]
2AT1X-ray2.80B/D1-153[»]
2ATCX-ray3.00B1-153[»]
2FZCX-ray2.10B/D1-153[»]
2FZGX-ray2.25B/D1-153[»]
2FZKX-ray2.50B/D1-153[»]
2H3EX-ray2.30B/D1-153[»]
2HSEX-ray2.60B/D1-153[»]
2IPOX-ray2.60B/D1-153[»]
2QG9X-ray2.70B/D1-153[»]
2QGFX-ray2.20B/D1-153[»]
3AT1X-ray2.80B/D1-153[»]
3D7SX-ray2.80B/D1-153[»]
3MPUX-ray2.86B/D/F1-153[»]
4AT1X-ray2.60B/D1-153[»]
4E2FX-ray2.80B/D/F/H/J/L1-153[»]
4F04X-ray2.30B/D1-153[»]
4FYVX-ray2.10B/D1-153[»]
4FYWX-ray2.10B/D1-153[»]
4FYXX-ray2.09B/D1-153[»]
4FYYX-ray1.94B/D1-153[»]
4WTOX-ray2.03B/D1-153[»]
5AT1X-ray2.60B/D1-153[»]
6AT1X-ray2.60B/D1-153[»]
7AT1X-ray2.80B/D1-153[»]
8AT1X-ray2.80B/D1-153[»]
8ATCX-ray2.50B/D1-153[»]
9ATCX-ray2.40B8-153[»]
ProteinModelPortaliP0A7F3.
SMRiP0A7F3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263117. 2 interactors.
DIPiDIP-35088N.
IntActiP0A7F3. 2 interactors.
MINTiMINT-1541364.
STRINGi511145.b4244.

2D gel databases

SWISS-2DPAGEP0A7F3.

Proteomic databases

EPDiP0A7F3.
PaxDbiP0A7F3.
PRIDEiP0A7F3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77201; AAC77201; b4244.
BAE78243; BAE78243; BAE78243.
GeneIDi948763.
KEGGiecj:JW4203.
eco:b4244.
PATRICi32124065. VBIEscCol129921_4377.

Organism-specific databases

EchoBASEiEB0804.
EcoGeneiEG10811. pyrI.

Phylogenomic databases

eggNOGiENOG4108R9M. Bacteria.
COG1781. LUCA.
HOGENOMiHOG000113530.
InParanoidiP0A7F3.
KOiK00610.
OMAiMNVPSDR.
PhylomeDBiP0A7F3.

Enzyme and pathway databases

BioCyciEcoCyc:ASPCARBREG-MONOMER.
ECOL316407:JW4203-MONOMER.
MetaCyc:ASPCARBREG-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A7F3.
PROiP0A7F3.

Family and domain databases

Gene3Di2.30.30.20. 1 hit.
3.30.70.140. 1 hit.
HAMAPiMF_00002. Asp_carb_tr_reg. 1 hit.
InterProiIPR020545. Asp_carbamoyltransf_reg_N.
IPR002801. Asp_carbamoylTrfase_reg.
IPR020542. Asp_carbamoyltrfase_reg_C.
[Graphical view]
PfamiPF01948. PyrI. 1 hit.
PF02748. PyrI_C. 1 hit.
[Graphical view]
ProDomiPD006194. Asp_carbamoylTrfase_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54893. SSF54893. 1 hit.
SSF57825. SSF57825. 1 hit.
TIGRFAMsiTIGR00240. ATCase_reg. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRI_ECOLI
AccessioniPrimary (citable) accession number: P0A7F3
Secondary accession number(s): P00478, Q2M663
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.