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Protein

Uridylate kinase

Gene

pyrH

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.UniRule annotation

Catalytic activityi

ATP + UMP = ADP + UDP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP. Inhibited by UTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571UMP; via amide nitrogenUniRule annotation
Binding sitei58 – 581ATP; via amide nitrogenUniRule annotation
Binding sitei62 – 621ATPUniRule annotation
Binding sitei77 – 771UMPUniRule annotation
Binding sitei165 – 1651ATPUniRule annotation
Binding sitei171 – 1711ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei174 – 1741ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 184ATPUniRule annotation
Nucleotide bindingi138 – 1458UMPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-171-MONOMER.
ECOO157:PYRH-MONOMER.
UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinaseUniRule annotation (EC:2.7.4.22UniRule annotation)
Short name:
UKUniRule annotation
Alternative name(s):
Uridine monophosphate kinaseUniRule annotation
Short name:
UMP kinaseUniRule annotation
Short name:
UMPKUniRule annotation
Gene namesi
Name:pyrHUniRule annotation
Ordered Locus Names:Z0182, ECs0173
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 241240Uridylate kinasePRO_0000143843Add
BLAST

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

MINTiMINT-1222932.
STRINGi155864.Z0182.

Structurei

3D structure databases

ProteinModelPortaliP0A7F1.
SMRiP0A7F1. Positions 3-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 286Involved in allosteric activation by GTPUniRule annotation

Sequence similaritiesi

Belongs to the UMP kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C41. Bacteria.
COG0528. LUCA.
HOGENOMiHOG000047187.
KOiK09903.
OMAiIIVFDMN.
OrthoDBiEOG6M0T8S.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNAKPVYK RILLKLSGEA LQGTEGFGID ASILDRMAQE IKELVELGIQ
60 70 80 90 100
VGVVIGGGNL FRGAGLAKAG MNRVVGDHMG MLATVMNGLA MRDALHRAYV
110 120 130 140 150
NARLMSAIPL NGVCDSYSWA EAISLLRNNR VVILSAGTGN PFFTTDSAAC
160 170 180 190 200
LRGIEIEADV VLKATKVDGV FTADPAKDPT ATMYEQLTYS EVLEKELKVM
210 220 230 240
DLAAFTLARD HKLPIRVFNM NKPGALRRVV MGEKEGTLIT E
Length:241
Mass (Da):25,970
Last modified:January 23, 2007 - v2
Checksum:i82EFA75F7226E201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54473.1.
BA000007 Genomic DNA. Translation: BAB33596.1.
PIRiE85501.
E90650.
RefSeqiNP_308200.1. NC_002695.1.
WP_000224573.1. NZ_LPWC01000161.1.

Genome annotation databases

EnsemblBacteriaiAAG54473; AAG54473; Z0182.
BAB33596; BAB33596; BAB33596.
GeneIDi913857.
KEGGiece:Z0182.
ecs:ECs0173.
PATRICi18349290. VBIEscCol44059_0175.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54473.1.
BA000007 Genomic DNA. Translation: BAB33596.1.
PIRiE85501.
E90650.
RefSeqiNP_308200.1. NC_002695.1.
WP_000224573.1. NZ_LPWC01000161.1.

3D structure databases

ProteinModelPortaliP0A7F1.
SMRiP0A7F1. Positions 3-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1222932.
STRINGi155864.Z0182.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54473; AAG54473; Z0182.
BAB33596; BAB33596; BAB33596.
GeneIDi913857.
KEGGiece:Z0182.
ecs:ECs0173.
PATRICi18349290. VBIEscCol44059_0175.

Phylogenomic databases

eggNOGiENOG4105C41. Bacteria.
COG0528. LUCA.
HOGENOMiHOG000047187.
KOiK09903.
OMAiIIVFDMN.
OrthoDBiEOG6M0T8S.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.
BioCyciECOL386585:GJFA-171-MONOMER.
ECOO157:PYRH-MONOMER.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPYRH_ECO57
AccessioniPrimary (citable) accession number: P0A7F1
Secondary accession number(s): P29464
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.