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P0A7E3

- PYRE_ECOLI

UniProt

P0A7E3 - PYRE_ECOLI

Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).By similarity

    Catalytic activityi

    Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Inhibited by sulfate and phosphate ions.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei26 – 2615-phosphoribose 1-diphosphateBy similarity
    Binding sitei99 – 9915-phosphoribose 1-diphosphate; shared with dimeric partner
    Binding sitei100 – 10015-phosphoribose 1-diphosphate
    Binding sitei103 – 10315-phosphoribose 1-diphosphate; shared with dimeric partner
    Binding sitei105 – 10515-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
    Binding sitei128 – 1281OrotateBy similarity
    Binding sitei156 – 1561OrotateBy similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: EcoCyc
    2. orotate phosphoribosyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: EcoCyc
    2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    3. pyrimidine nucleotide biosynthetic process Source: EcoliWiki

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciEcoCyc:OROPRIBTRANS-MONOMER.
    ECOL316407:JW3617-MONOMER.
    MetaCyc:OROPRIBTRANS-MONOMER.
    UniPathwayiUPA00070; UER00119.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Orotate phosphoribosyltransferase (EC:2.4.2.10)
    Short name:
    OPRT
    Short name:
    OPRTase
    Gene namesi
    Name:pyrE
    Ordered Locus Names:b3642, JW3617
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10808. pyrE.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: EcoliWiki

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 213212Orotate phosphoribosyltransferasePRO_0000110694Add
    BLAST

    Proteomic databases

    PRIDEiP0A7E3.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A7E3.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    IntActiP0A7E3. 1 interaction.
    STRINGi511145.b3642.

    Structurei

    Secondary structure

    1
    213
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1412
    Beta strandi17 – 248
    Beta strandi30 – 356
    Helixi37 – 393
    Helixi43 – 6018
    Beta strandi65 – 695
    Turni71 – 733
    Helixi74 – 8916
    Beta strandi94 – 985
    Beta strandi104 – 1074
    Beta strandi111 – 1144
    Beta strandi118 – 1236
    Helixi130 – 1334
    Helixi134 – 1429
    Beta strandi146 – 15510
    Beta strandi161 – 1644
    Helixi166 – 1749
    Beta strandi177 – 1837
    Helixi184 – 1918
    Helixi195 – 1973
    Helixi198 – 21114

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OROX-ray2.40A/B1-213[»]
    ProteinModelPortaliP0A7E3.
    SMRiP0A7E3. Positions 1-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A7E3.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni34 – 352Orotate bindingBy similarity
    Regioni72 – 7325-phosphoribose 1-diphosphate binding
    Regioni124 – 13295-phosphoribose 1-diphosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0461.
    HOGENOMiHOG000037974.
    KOiK00762.
    OMAiPYFFNTG.
    OrthoDBiEOG6091H8.
    PhylomeDBiP0A7E3.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    HAMAPiMF_01208. PyrE.
    InterProiIPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR00336. pyrE. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A7E3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPYQRQFIE FALSKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLALLG    50
    RFYAEALVDS GIEFDLLFGP AYKGIPIATT TAVALAEHHD LDLPYCFNRK 100
    EAKDHGEGGN LVGSALQGRV MLVDDVITAG TAIRESMEII QANGATLAGV 150
    LISLDRQERG RGEISAIQEV ERDYNCKVIS IITLKDLIAY LEEKPEMAEH 200
    LAAVKAYREE FGV 213
    Length:213
    Mass (Da):23,567
    Last modified:January 23, 2007 - v2
    Checksum:iF618A6D0A119B9D4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti133 – 1331Missing(PubMed:6349999)Curated
    Sequence conflicti133 – 1331Missing(PubMed:6207018)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00781 Genomic DNA. Translation: CAA25358.1.
    V01578 Genomic DNA. Translation: CAA24899.1.
    L10328 Genomic DNA. Translation: AAA61995.1.
    U00096 Genomic DNA. Translation: AAC76666.1.
    AP009048 Genomic DNA. Translation: BAE77650.1.
    PIRiD65165. XJEC.
    RefSeqiNP_418099.1. NC_000913.3.
    YP_491791.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76666; AAC76666; b3642.
    BAE77650; BAE77650; BAE77650.
    GeneIDi12930578.
    948157.
    KEGGiecj:Y75_p3532.
    eco:b3642.
    PATRICi32122771. VBIEscCol129921_3762.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00781 Genomic DNA. Translation: CAA25358.1 .
    V01578 Genomic DNA. Translation: CAA24899.1 .
    L10328 Genomic DNA. Translation: AAA61995.1 .
    U00096 Genomic DNA. Translation: AAC76666.1 .
    AP009048 Genomic DNA. Translation: BAE77650.1 .
    PIRi D65165. XJEC.
    RefSeqi NP_418099.1. NC_000913.3.
    YP_491791.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ORO X-ray 2.40 A/B 1-213 [» ]
    ProteinModelPortali P0A7E3.
    SMRi P0A7E3. Positions 1-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0A7E3. 1 interaction.
    STRINGi 511145.b3642.

    Proteomic databases

    PRIDEi P0A7E3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76666 ; AAC76666 ; b3642 .
    BAE77650 ; BAE77650 ; BAE77650 .
    GeneIDi 12930578.
    948157.
    KEGGi ecj:Y75_p3532.
    eco:b3642.
    PATRICi 32122771. VBIEscCol129921_3762.

    Organism-specific databases

    EchoBASEi EB0801.
    EcoGenei EG10808. pyrE.

    Phylogenomic databases

    eggNOGi COG0461.
    HOGENOMi HOG000037974.
    KOi K00762.
    OMAi PYFFNTG.
    OrthoDBi EOG6091H8.
    PhylomeDBi P0A7E3.

    Enzyme and pathway databases

    UniPathwayi UPA00070 ; UER00119 .
    BioCyci EcoCyc:OROPRIBTRANS-MONOMER.
    ECOL316407:JW3617-MONOMER.
    MetaCyc:OROPRIBTRANS-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A7E3.
    PROi P0A7E3.

    Gene expression databases

    Genevestigatori P0A7E3.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    HAMAPi MF_01208. PyrE.
    InterProi IPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR00336. pyrE. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region."
      Poulsen P., Jensen K.F., Valentin-Hansen P., Carlsson P., Lundberg L.G.
      Eur. J. Biochem. 135:223-229(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, SUBUNIT.
      Strain: K12.
    2. "Structure of the Escherichia coli pyrE operon and control of pyrE expression by a UTP modulated intercistronic attentuation."
      Poulsen P., Bonekamp F., Jensen K.F.
      EMBO J. 3:1783-1790(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
      Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
      Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    7. "A flexible loop at the dimer interface is a part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase."
      Henriksen A., Aghajari N., Jensen K.F., Gajhede M.
      Biochemistry 35:3803-3809(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH DIPHOSPHATE ANALOG, FUNCTION, ENZYME REGULATION, SUBUNIT.

    Entry informationi

    Entry nameiPYRE_ECOLI
    AccessioniPrimary (citable) accession number: P0A7E3
    Secondary accession number(s): P00495, Q2M7V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3