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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation1 Publication

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Inhibited by sulfate and phosphate ions.1 Publication

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265-phosphoribose 1-diphosphateUniRule annotation1
Binding sitei995-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1 Publication1
Binding sitei1005-phosphoribose 1-diphosphateUniRule annotation1 Publication1
Binding sitei1035-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1 Publication1
Binding sitei1055-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei128OrotateUniRule annotation1
Binding sitei156OrotateUniRule annotation1

GO - Molecular functioni

  • magnesium ion binding Source: EcoCyc
  • orotate phosphoribosyltransferase activity Source: EcoCyc

GO - Biological processi

  • 'de novo' pyrimidine nucleobase biosynthetic process Source: EcoCyc
  • 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  • pyrimidine nucleotide biosynthetic process Source: EcoliWiki

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPyrimidine biosynthesis
LigandMagnesium

Enzyme and pathway databases

BioCyciEcoCyc:OROPRIBTRANS-MONOMER
MetaCyc:OROPRIBTRANS-MONOMER
UniPathwayiUPA00070; UER00119

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrE1 Publication
Ordered Locus Names:b3642, JW3617
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10808 pyrE

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001106942 – 213Orotate phosphoribosyltransferaseAdd BLAST212

Proteomic databases

EPDiP0A7E3
PaxDbiP0A7E3
PRIDEiP0A7E3

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi4259572, 15 interactors
IntActiP0A7E3, 1 interactor
STRINGi316385.ECDH10B_3824

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Beta strandi17 – 24Combined sources8
Beta strandi30 – 35Combined sources6
Helixi37 – 39Combined sources3
Helixi43 – 60Combined sources18
Beta strandi65 – 69Combined sources5
Turni71 – 73Combined sources3
Helixi74 – 89Combined sources16
Beta strandi94 – 98Combined sources5
Beta strandi104 – 107Combined sources4
Beta strandi111 – 114Combined sources4
Beta strandi118 – 123Combined sources6
Helixi130 – 133Combined sources4
Helixi134 – 142Combined sources9
Beta strandi146 – 155Combined sources10
Beta strandi161 – 164Combined sources4
Helixi166 – 174Combined sources9
Beta strandi177 – 183Combined sources7
Helixi184 – 191Combined sources8
Helixi195 – 197Combined sources3
Helixi198 – 211Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OROX-ray2.40A/B1-213[»]
ProteinModelPortaliP0A7E3
SMRiP0A7E3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7E3

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 35Orotate bindingUniRule annotation2
Regioni72 – 735-phosphoribose 1-diphosphate bindingUniRule annotation1 Publication2
Regioni124 – 1325-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP2 Bacteria
COG0461 LUCA
HOGENOMiHOG000037974
InParanoidiP0A7E3
KOiK00762
OMAiMKAYQRQ
PhylomeDBiP0A7E3

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_01208 PyrE, 1 hit
InterProiView protein in InterPro
IPR023031 OPRT
IPR004467 Or_phspho_trans_dom
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR00336 pyrE, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7E3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPYQRQFIE FALSKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLALLG
60 70 80 90 100
RFYAEALVDS GIEFDLLFGP AYKGIPIATT TAVALAEHHD LDLPYCFNRK
110 120 130 140 150
EAKDHGEGGN LVGSALQGRV MLVDDVITAG TAIRESMEII QANGATLAGV
160 170 180 190 200
LISLDRQERG RGEISAIQEV ERDYNCKVIS IITLKDLIAY LEEKPEMAEH
210
LAAVKAYREE FGV
Length:213
Mass (Da):23,567
Last modified:January 23, 2007 - v2
Checksum:iF618A6D0A119B9D4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133Missing (PubMed:6349999).Curated1
Sequence conflicti133Missing (PubMed:6207018).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00781 Genomic DNA Translation: CAA25358.1
V01578 Genomic DNA Translation: CAA24899.1
L10328 Genomic DNA Translation: AAA61995.1
U00096 Genomic DNA Translation: AAC76666.1
AP009048 Genomic DNA Translation: BAE77650.1
PIRiD65165 XJEC
RefSeqiNP_418099.1, NC_000913.3
WP_000806177.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76666; AAC76666; b3642
BAE77650; BAE77650; BAE77650
GeneIDi948157
KEGGiecj:JW3617
eco:b3642
PATRICifig|1411691.4.peg.3064

Similar proteinsi

Entry informationi

Entry nameiPYRE_ECOLI
AccessioniPrimary (citable) accession number: P0A7E3
Secondary accession number(s): P00495, Q2M7V6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health