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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene

purC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC)
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:SAICARSYN-MONOMER
MetaCyc:SAICARSYN-MONOMER
BRENDAi6.3.2.6 2026
UniPathwayiUPA00074; UER00131

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Gene namesi
Name:purC
Ordered Locus Names:b2476, JW2461
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10791 purC

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • membrane Source: UniProtKB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001008251 – 237Phosphoribosylaminoimidazole-succinocarboxamide synthaseAdd BLAST237

Proteomic databases

EPDiP0A7D7
PaxDbiP0A7D7
PRIDEiP0A7D7

2D gel databases

SWISS-2DPAGEiP0A7D7

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

BioGridi4261983, 44 interactors
DIPiDIP-35900N
IntActiP0A7D7, 17 interactors
STRINGi316385.ECDH10B_2642

Structurei

Secondary structure

1237
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi11 – 17Combined sources7
Beta strandi23 – 28Combined sources6
Beta strandi30 – 32Combined sources3
Turni35 – 38Combined sources4
Beta strandi39 – 42Combined sources4
Helixi46 – 63Combined sources18
Beta strandi70 – 73Combined sources4
Beta strandi75 – 82Combined sources8
Beta strandi88 – 96Combined sources9
Helixi99 – 105Combined sources7
Beta strandi112 – 122Combined sources11
Helixi125 – 127Combined sources3
Helixi134 – 139Combined sources6
Helixi145 – 168Combined sources24
Beta strandi171 – 183Combined sources13
Beta strandi186 – 191Combined sources6
Beta strandi197 – 202Combined sources6
Turni203 – 205Combined sources3
Helixi212 – 215Combined sources4
Helixi221 – 232Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GQRX-ray2.00A/B1-237[»]
2GQSX-ray2.05A/B1-237[»]
ProteinModelPortaliP0A7D7
SMRiP0A7D7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7D7

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C8V Bacteria
COG0152 LUCA
HOGENOMiHOG000082629
InParanoidiP0A7D7
KOiK01923
OMAiFNAQKRG
PhylomeDBiP0A7D7

Family and domain databases

CDDicd01415 SAICAR_synt_PurC, 1 hit
HAMAPiMF_00137 SAICAR_synth, 1 hit
InterProiView protein in InterPro
IPR028923 SAICAR_synt/ADE2_N
IPR033934 SAICAR_synt_PurC
IPR001636 SAICAR_synth
IPR018236 SAICAR_synthetase_CS
PfamiView protein in Pfam
PF01259 SAICAR_synt, 1 hit
TIGRFAMsiTIGR00081 purC, 1 hit
PROSITEiView protein in PROSITE
PS01057 SAICAR_SYNTHETASE_1, 1 hit
PS01058 SAICAR_SYNTHETASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A7D7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKQAELYRG KAKTVYSTEN PDLLVLEFRN DTSAGDGARI EQFDRKGMVN
60 70 80 90 100
NKFNYFIMSK LAEAGIPTQM ERLLSDTECL VKKLDMVPVE CVVRNRAAGS
110 120 130 140 150
LVKRLGIEEG IELNPPLFDL FLKNDAMHDP MVNESYCETF GWVSKENLAR
160 170 180 190 200
MKELTYKAND VLKKLFDDAG LILVDFKLEF GLYKGEVVLG DEFSPDGSRL
210 220 230
WDKETLEKMD KDRFRQSLGG LIEAYEAVAR RLGVQLD
Length:237
Mass (Da):26,995
Last modified:June 7, 2005 - v1
Checksum:iE5D0E69E84E35C97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33928 Genomic DNA Translation: AAA24448.1
U00096 Genomic DNA Translation: AAC75529.1
AP009048 Genomic DNA Translation: BAA16353.1
X57402 Genomic DNA Translation: CAA40662.1
PIRiC36146
RefSeqiNP_416971.1, NC_000913.3
WP_001295467.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75529; AAC75529; b2476
BAA16353; BAA16353; BAA16353
GeneIDi946957
KEGGiecj:JW2461
eco:b2476
PATRICifig|1411691.4.peg.4263

Similar proteinsi

Entry informationi

Entry nameiPUR7_ECOLI
AccessioniPrimary (citable) accession number: P0A7D7
Secondary accession number(s): P21155
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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