Phosphoribosylaminoimidazole-succinocarboxamide synthase

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P0A7D7 (PUR7_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 66. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	b2476, JW2461

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Subunit structure	Homotrimer.
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB membrane Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 237

237

Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137

PRO_0000100825

Secondary structure

237

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A7D7 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: E5D0E69E84E35C97
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FASTA

237

26,995

        10         20         30         40         50         60 
MQKQAELYRG KAKTVYSTEN PDLLVLEFRN DTSAGDGARI EQFDRKGMVN NKFNYFIMSK 

        70         80         90        100        110        120 
LAEAGIPTQM ERLLSDTECL VKKLDMVPVE CVVRNRAAGS LVKRLGIEEG IELNPPLFDL 

       130        140        150        160        170        180 
FLKNDAMHDP MVNESYCETF GWVSKENLAR MKELTYKAND VLKKLFDDAG LILVDFKLEF 

       190        200        210        220        230 
GLYKGEVVLG DEFSPDGSRL WDKETLEKMD KDRFRQSLGG LIEAYEAVAR RLGVQLD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12." Tiedemann A.A., Demarini D.J., Parker J., Smith J.M. J. Bacteriol. 172:6035-6041(1990) [PubMed: 2120198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome." Bouvier J., Pugsley A.P., Stragier P. J. Bacteriol. 173:5523-5531(1991) [PubMed: 1885529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28. Strain: K12.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
[7]	"Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway." Meyer E., Leonard N.J., Bhat B., Stubbe J., Smith J.M. Biochemistry 31:5022-5032(1992) [PubMed: 1534690] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M33928 Genomic DNA. Translation: AAA24448.1.
U00096 Genomic DNA. Translation: AAC75529.1.
AP009048 Genomic DNA. Translation: BAA16353.1.
X57402 Genomic DNA. Translation: CAA40662.1.

PIR

C36146.

RefSeq

NP_416971.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GQR	X-ray	2.00	A/B	1-237	[»]
2GQS	X-ray	2.05	A/B	1-237	[»]

ProteinModelPortal

P0A7D7.

SMR

P0A7D7. Positions 1-237.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35900N.

IntAct

P0A7D7. 19 interactions.

MINT

MINT-1257522.

2D gel databases

SWISS-2DPAGE

P0A7D7.

ECO2DBASE

B026.3. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000003853; EBESCP00000003853; EBESCG00000003148.
EBESCT00000014498; EBESCP00000013789; EBESCG00000013559.

GeneID

946957.

GenomeReviews

Gene locus JW2461 in contig AP009048_GR.
Gene locus b2476 in contig U00096_GR.

KEGG

ecj:JW2461.
eco:b2476.

PATRIC

32120337. VBIEscCol129921_2571.

Organism-specific databases

EchoBASE

EB0784.

EcoGene

EG10791. purC.

Phylogenomic databases

eggNOG

COG0152.

GeneTree

EBGT00050000009817.

HOGENOM

HBG306070.

OMA

YKDDALG.

PhylomeDB

P0A7D7.

ProtClustDB

PRK09362.

Enzyme and pathway databases

BioCyc

EcoCyc:SAICARSYN-MONOMER.
MetaCyc:SAICARSYN-MONOMER.

Gene expression databases

Genevestigator

P0A7D7.

Family and domain databases

HAMAP

MF_00137. SAICAR_synth.
[Tree]

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.

K01923.

PANTHER

PTHR11609. SAICAR_synt. 1 hit.

Pfam

PF01259. SAICAR_synt. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00081. PurC. 1 hit.

PROSITE

PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PUR7_ECOLI

Accession

Primary (citable) accession number: P0A7D7
Secondary accession number(s): P21155

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents