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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene

purC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC)
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:SAICARSYN-MONOMER.
ECOL316407:JW2461-MONOMER.
MetaCyc:SAICARSYN-MONOMER.
BRENDAi6.3.2.6. 2026.
UniPathwayiUPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Gene namesi
Name:purC
Ordered Locus Names:b2476, JW2461
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10791. purC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Phosphoribosylaminoimidazole-succinocarboxamide synthasePRO_0000100825Add
BLAST

Proteomic databases

EPDiP0A7D7.
PaxDbiP0A7D7.
PRIDEiP0A7D7.

2D gel databases

SWISS-2DPAGEP0A7D7.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

BioGridi4261983. 23 interactions.
DIPiDIP-35900N.
IntActiP0A7D7. 17 interactions.
MINTiMINT-1257522.
STRINGi511145.b2476.

Structurei

Secondary structure

1
237
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Beta strandi11 – 177Combined sources
Beta strandi23 – 286Combined sources
Beta strandi30 – 323Combined sources
Turni35 – 384Combined sources
Beta strandi39 – 424Combined sources
Helixi46 – 6318Combined sources
Beta strandi70 – 734Combined sources
Beta strandi75 – 828Combined sources
Beta strandi88 – 969Combined sources
Helixi99 – 1057Combined sources
Beta strandi112 – 12211Combined sources
Helixi125 – 1273Combined sources
Helixi134 – 1396Combined sources
Helixi145 – 16824Combined sources
Beta strandi171 – 18313Combined sources
Beta strandi186 – 1916Combined sources
Beta strandi197 – 2026Combined sources
Turni203 – 2053Combined sources
Helixi212 – 2154Combined sources
Helixi221 – 23212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GQRX-ray2.00A/B1-237[»]
2GQSX-ray2.05A/B1-237[»]
ProteinModelPortaliP0A7D7.
SMRiP0A7D7. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7D7.

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C8V. Bacteria.
COG0152. LUCA.
HOGENOMiHOG000082629.
InParanoidiP0A7D7.
KOiK01923.
OMAiFNAQKRG.
PhylomeDBiP0A7D7.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A7D7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKQAELYRG KAKTVYSTEN PDLLVLEFRN DTSAGDGARI EQFDRKGMVN
60 70 80 90 100
NKFNYFIMSK LAEAGIPTQM ERLLSDTECL VKKLDMVPVE CVVRNRAAGS
110 120 130 140 150
LVKRLGIEEG IELNPPLFDL FLKNDAMHDP MVNESYCETF GWVSKENLAR
160 170 180 190 200
MKELTYKAND VLKKLFDDAG LILVDFKLEF GLYKGEVVLG DEFSPDGSRL
210 220 230
WDKETLEKMD KDRFRQSLGG LIEAYEAVAR RLGVQLD
Length:237
Mass (Da):26,995
Last modified:June 7, 2005 - v1
Checksum:iE5D0E69E84E35C97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33928 Genomic DNA. Translation: AAA24448.1.
U00096 Genomic DNA. Translation: AAC75529.1.
AP009048 Genomic DNA. Translation: BAA16353.1.
X57402 Genomic DNA. Translation: CAA40662.1.
PIRiC36146.
RefSeqiNP_416971.1. NC_000913.3.
WP_001295467.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75529; AAC75529; b2476.
BAA16353; BAA16353; BAA16353.
GeneIDi946957.
KEGGiecj:JW2461.
eco:b2476.
PATRICi32120337. VBIEscCol129921_2571.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33928 Genomic DNA. Translation: AAA24448.1.
U00096 Genomic DNA. Translation: AAC75529.1.
AP009048 Genomic DNA. Translation: BAA16353.1.
X57402 Genomic DNA. Translation: CAA40662.1.
PIRiC36146.
RefSeqiNP_416971.1. NC_000913.3.
WP_001295467.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GQRX-ray2.00A/B1-237[»]
2GQSX-ray2.05A/B1-237[»]
ProteinModelPortaliP0A7D7.
SMRiP0A7D7. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261983. 23 interactions.
DIPiDIP-35900N.
IntActiP0A7D7. 17 interactions.
MINTiMINT-1257522.
STRINGi511145.b2476.

2D gel databases

SWISS-2DPAGEP0A7D7.

Proteomic databases

EPDiP0A7D7.
PaxDbiP0A7D7.
PRIDEiP0A7D7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75529; AAC75529; b2476.
BAA16353; BAA16353; BAA16353.
GeneIDi946957.
KEGGiecj:JW2461.
eco:b2476.
PATRICi32120337. VBIEscCol129921_2571.

Organism-specific databases

EchoBASEiEB0784.
EcoGeneiEG10791. purC.

Phylogenomic databases

eggNOGiENOG4105C8V. Bacteria.
COG0152. LUCA.
HOGENOMiHOG000082629.
InParanoidiP0A7D7.
KOiK01923.
OMAiFNAQKRG.
PhylomeDBiP0A7D7.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00131.
BioCyciEcoCyc:SAICARSYN-MONOMER.
ECOL316407:JW2461-MONOMER.
MetaCyc:SAICARSYN-MONOMER.
BRENDAi6.3.2.6. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A7D7.
PROiP0A7D7.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth. 1 hit.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR7_ECOLI
AccessioniPrimary (citable) accession number: P0A7D7
Secondary accession number(s): P21155
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.