Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peptidase E

Gene

pepE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes dipeptides containing N-terminal aspartate residues. May play a role in allowing the cell to use peptide aspartate to spare carbon otherwise required for the synthesis of the aspartate family of amino acids.UniRule annotation

Catalytic activityi

Dipeptidase E catalyzes the hydrolysis of dipeptides Asp-|-Xaa. It does not act on peptides with N-terminal Glu, Asn or Gln, nor does it cleave isoaspartyl peptides.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei120 – 1201Charge relay systemUniRule annotation
Active sitei135 – 1351Charge relay systemUniRule annotation
Active sitei157 – 1571Charge relay systemUniRule annotation

GO - Molecular functioni

  1. dipeptidase activity Source: UniProtKB-HAMAP
  2. peptidase activity Source: EcoCyc
  3. serine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG11920-MONOMER.
ECOL316407:JW3981-MONOMER.
MetaCyc:EG11920-MONOMER.

Protein family/group databases

MEROPSiS51.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase EUniRule annotation (EC:3.4.13.21UniRule annotation)
Alternative name(s):
Alpha-aspartyl dipeptidaseUniRule annotation
Asp-specific dipeptidaseUniRule annotation
Dipeptidase EUniRule annotation
Gene namesi
Name:pepEUniRule annotation
Ordered Locus Names:b4021, JW3981
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11920. pepE.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229Peptidase EPRO_0000209955Add
BLAST

Proteomic databases

PaxDbiP0A7C6.
PRIDEiP0A7C6.

Expressioni

Gene expression databases

GenevestigatoriP0A7C6.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
pepBP370951EBI-555623,EBI-549539

Protein-protein interaction databases

DIPiDIP-48072N.
IntActiP0A7C6. 4 interactions.
MINTiMINT-1236370.
STRINGi511145.b4021.

Structurei

3D structure databases

ProteinModelPortaliP0A7C6.
SMRiP0A7C6. Positions 1-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S51 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3340.
HOGENOMiHOG000281834.
InParanoidiP0A7C6.
KOiK05995.
OMAiTPYIGWS.
OrthoDBiEOG6M6JST.
PhylomeDBiP0A7C6.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00510. Peptidase_E.
InterProiIPR029062. Class_I_gatase-like.
IPR005320. Peptidase_S51.
IPR023172. Peptidase_S51_dipeptidase-E.
[Graphical view]
PfamiPF03575. Peptidase_S51. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A7C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLLLSNST LPGKAWLEHA LPLIAEQLQG RRSAVFIPFA GVTQTWDDYT
60 70 80 90 100
AKTAAVLAPL GVSVTGIHSV VDPVAAIENA EIVIVGGGNT FQLLKQCRER
110 120 130 140 150
GLLAPITDVV KRGALYIGWS AGANLACPTI RTTNDMPIVD PQGFDALNLF
160 170 180 190 200
PLQINPHFTN ALPEGHKGET REQRIRELLV VAPELTIIGL PEGNWITVSK
210 220
GHATLGGPNT TYVFKAGEEA VPLEAGHRF
Length:229
Mass (Da):24,570
Last modified:June 7, 2005 - v1
Checksum:i53D4D8395DFC63FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43115.1.
U00096 Genomic DNA. Translation: AAC76991.1.
AP009048 Genomic DNA. Translation: BAE78023.1.
PIRiD65209.
RefSeqiNP_418445.1. NC_000913.3.
YP_492164.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76991; AAC76991; b4021.
BAE78023; BAE78023; BAE78023.
GeneIDi12933661.
948520.
KEGGiecj:Y75_p3908.
eco:b4021.
PATRICi32123571. VBIEscCol129921_4134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43115.1.
U00096 Genomic DNA. Translation: AAC76991.1.
AP009048 Genomic DNA. Translation: BAE78023.1.
PIRiD65209.
RefSeqiNP_418445.1. NC_000913.3.
YP_492164.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0A7C6.
SMRiP0A7C6. Positions 1-229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48072N.
IntActiP0A7C6. 4 interactions.
MINTiMINT-1236370.
STRINGi511145.b4021.

Protein family/group databases

MEROPSiS51.001.

Proteomic databases

PaxDbiP0A7C6.
PRIDEiP0A7C6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76991; AAC76991; b4021.
BAE78023; BAE78023; BAE78023.
GeneIDi12933661.
948520.
KEGGiecj:Y75_p3908.
eco:b4021.
PATRICi32123571. VBIEscCol129921_4134.

Organism-specific databases

EchoBASEiEB1864.
EcoGeneiEG11920. pepE.

Phylogenomic databases

eggNOGiCOG3340.
HOGENOMiHOG000281834.
InParanoidiP0A7C6.
KOiK05995.
OMAiTPYIGWS.
OrthoDBiEOG6M6JST.
PhylomeDBiP0A7C6.

Enzyme and pathway databases

BioCyciEcoCyc:EG11920-MONOMER.
ECOL316407:JW3981-MONOMER.
MetaCyc:EG11920-MONOMER.

Miscellaneous databases

PROiP0A7C6.

Gene expression databases

GenevestigatoriP0A7C6.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00510. Peptidase_E.
InterProiIPR029062. Class_I_gatase-like.
IPR005320. Peptidase_S51.
IPR023172. Peptidase_S51_dipeptidase-E.
[Graphical view]
PfamiPF03575. Peptidase_S51. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPEPE_ECOLI
AccessioniPrimary (citable) accession number: P0A7C6
Secondary accession number(s): P32666, Q2M6T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.