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P0A7C6 (PEPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidase E

EC=3.4.13.21
Alternative name(s):
Alpha-aspartyl dipeptidase
Asp-specific dipeptidase
Dipeptidase E
Gene names
Name:pepE
Ordered Locus Names:b4021, JW3981
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes dipeptides containing N-terminal aspartate residues. May play a role in allowing the cell to use peptide aspartate to spare carbon otherwise required for the synthesis of the aspartate family of amino acids By similarity. HAMAP-Rule MF_00510

Catalytic activity

Dipeptidase E catalyzes the hydrolysis of dipeptides Asp-|-Xaa. It does not act on peptides with N-terminal Glu, Asn or Gln, nor does it cleave isoaspartyl peptides. HAMAP-Rule MF_00510

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00510.

Sequence similarities

Belongs to the peptidase S51 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionDipeptidase
Hydrolase
Protease
Serine protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondipeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

peptidase activity

Inferred from direct assay PubMed 9928148. Source: EcoCyc

serine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

pepBP370951EBI-555623,EBI-549539

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229Peptidase E HAMAP-Rule MF_00510
PRO_0000209955

Sites

Active site1201Charge relay system By similarity
Active site1351Charge relay system By similarity
Active site1571Charge relay system By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A7C6 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 53D4D8395DFC63FD

FASTA22924,570
        10         20         30         40         50         60 
MELLLLSNST LPGKAWLEHA LPLIAEQLQG RRSAVFIPFA GVTQTWDDYT AKTAAVLAPL 

        70         80         90        100        110        120 
GVSVTGIHSV VDPVAAIENA EIVIVGGGNT FQLLKQCRER GLLAPITDVV KRGALYIGWS 

       130        140        150        160        170        180 
AGANLACPTI RTTNDMPIVD PQGFDALNLF PLQINPHFTN ALPEGHKGET REQRIRELLV 

       190        200        210        220 
VAPELTIIGL PEGNWITVSK GHATLGGPNT TYVFKAGEEA VPLEAGHRF 

« Hide

References

[1]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00006 Genomic DNA. Translation: AAC43115.1.
U00096 Genomic DNA. Translation: AAC76991.1.
AP009048 Genomic DNA. Translation: BAE78023.1.
PIRD65209.
RefSeqNP_418445.1. NC_000913.3.
YP_492164.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A7C6.
SMRP0A7C6. Positions 1-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48072N.
IntActP0A7C6. 4 interactions.
MINTMINT-1236370.
STRING511145.b4021.

Protein family/group databases

MEROPSS51.001.

Proteomic databases

PaxDbP0A7C6.
PRIDEP0A7C6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76991; AAC76991; b4021.
BAE78023; BAE78023; BAE78023.
GeneID12933661.
948520.
KEGGecj:Y75_p3908.
eco:b4021.
PATRIC32123571. VBIEscCol129921_4134.

Organism-specific databases

EchoBASEEB1864.
EcoGeneEG11920. pepE.

Phylogenomic databases

eggNOGCOG3340.
HOGENOMHOG000281834.
KOK05995.
OMAHFISGKM.
OrthoDBEOG6M6JST.
PhylomeDBP0A7C6.
ProtClustDBPRK05282.

Enzyme and pathway databases

BioCycEcoCyc:EG11920-MONOMER.
ECOL316407:JW3981-MONOMER.
MetaCyc:EG11920-MONOMER.

Gene expression databases

GenevestigatorP0A7C6.

Family and domain databases

HAMAPMF_00510. Peptidase_E.
InterProIPR005320. Peptidase_S51.
IPR023172. Peptidase_S51_dipeptidase-E.
[Graphical view]
PfamPF03575. Peptidase_S51. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0A7C6.

Entry information

Entry namePEPE_ECOLI
AccessionPrimary (citable) accession number: P0A7C6
Secondary accession number(s): P32666, Q2M6T3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene