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Reviewed, UniProtKB/Swiss-Prot P0A7B3 (PPNK_ECOLI)

Last modified February 9, 2010. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Probable inorganic polyphosphate/ATP-NAD kinase
      Short name=Poly(P)/ATP NAD kinase
    EC=2.7.1.23
Gene names
Name: ppnK
Synonyms: yfjB, yfjE
Ordered Locus Names: b2615, JW2596
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP MF_00361

Subcellular location

Cytoplasm By similarity HAMAP MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Sequence caution

The sequence X07863 differs from that shown. Reason: Frameshift at several positions.

The sequence Y00357 differs from that shown. Reason: Frameshift at positions 204, 215 and 282.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processNADP biosynthetic process

Inferred from direct assay. Source: UniProtKB

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from direct assay. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361
PRO_0000120616

Sequences

Sequence LengthMass (Da)Tools
P0A7B3-1 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: D1E631658408F2E1

FASTA29232,566
        10         20         30         40         50         60 
MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CTKGYEVIVE QQIAHELQLK NVKTGTLAEI 

        70         80         90        100        110        120 
GQLADLAVVV GGDGNMLGAA RTLARYDIKV IGINRGNLGF LTDLDPDNAQ QQLADVLEGH 

       130        140        150        160        170        180 
YISEKRFLLE AQVCQQDCQK RISTAINEVV LHPGKVAHMI EFEVYIDEIF AFSQRSDGLI 

       190        200        210        220        230        240 
ISTPTGSTAY SLSAGGPILT PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRNDL 

       250        260        270        280        290 
EISCDSQIAL PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF 

« Hide

References

« Hide 'large scale' references
[1]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein."
Lipinska B., King J., Ang D., Georgopoulos C.
Nucleic Acids Res. 16:7545-7562(1988) [PubMed: 3045760] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-163.
Strain: B178.
[5]"Nucleotide sequence and LexA regulation of the Escherichia coli recN gene."
Rostas K., Morton S.J., Picksley S.M., Lloyd R.G.
Nucleic Acids Res. 15:5041-5049(1987) [PubMed: 3037486] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-292.
Strain: K12.
[6]"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
Borodovsky M., Rudd K.E., Koonin E.V.
Nucleic Acids Res. 22:4756-4767(1994) [PubMed: 7984428] [Abstract]
Cited for: IDENTIFICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U36840 Genomic DNA. Translation: AAA79785.1.
U00096 Genomic DNA. Translation: AAC75664.1.
AP009048 Genomic DNA. Translation: BAA16500.1.
X07863 Genomic DNA. No translation available.
Y00357 Genomic DNA. No translation available.
PIRB65040.
RefSeqAP_003195.1.
NP_417105.1.

3D structure databases

SMRP0A7B3. Positions 4-292.
ModBaseSearch...

Protein-protein interaction databases

STRINGP0A7B3.

Genome annotation databases

GeneID947092.
GenomeReviewsGene locus JW2596 in contig AP009048_GR.
Gene locus b2615 in contig U00096_GR.
KEGGecj:JW2596.
eco:b2615.

Organism-specific databases

EchoBASEEB2109.
EcoGeneEG12192. ppnK.
CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHBG713904.
OMAMEQFRNI.

Enzyme and pathway databases

BioCycEcoCyc:MONOMER0-541.
ECOL168927:B2615-MONOMER.
MetaCyc:MONOMER0-541.

Gene expression databases

GenevestigatorP0A7B3.

Family and domain databases

HAMAPMF_00361. NAD_kinase.
[Tree]
InterProIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. ATP_NADK.
[Graphical view]
Gene3DG3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit.
PANTHERPTHR20275. ATP_NADK. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPNK_ECOLI
AccessionPrimary (citable) accession number: P0A7B3
Secondary accession number(s): P37768, P46140, P77490
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Recent format changes

Overview of recent format changes

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents