Reviewed, UniProtKB/Swiss-Prot P0A7B3 (PPNK_ECOLI)

Names and origin

Protein names

Recommended name:
    Probable inorganic polyphosphate/ATP-NAD kinase
      Short name=Poly(P)/ATP NAD kinase
    EC=2.7.1.23

Gene names

Name:	ppnK
Synonyms:	yfjB, yfjE
Ordered Locus Names:	b2615, JW2596

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity. HAMAP MF_00361
Subcellular location	Cytoplasm By similarity HAMAP MF_00361.
Sequence similarities	Belongs to the NAD kinase family.
Sequence caution	The sequence X07863 differs from that shown. Reason: Frameshift at several positions. The sequence Y00357 differs from that shown. Reason: Frameshift at positions 204, 215 and 282.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP biosynthetic process Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from direct assay. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361		PRO_0000120616

Sequences

	Sequence		Length	Mass (Da)
	P0A7B3-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: D1E631658408F2E1	FASTA	292	32,566
	10 20 30 40 50 60 MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CTKGYEVIVE QQIAHELQLK NVKTGTLAEI 70 80 90 100 110 120 GQLADLAVVV GGDGNMLGAA RTLARYDIKV IGINRGNLGF LTDLDPDNAQ QQLADVLEGH 130 140 150 160 170 180 YISEKRFLLE AQVCQQDCQK RISTAINEVV LHPGKVAHMI EFEVYIDEIF AFSQRSDGLI 190 200 210 220 230 240 ISTPTGSTAY SLSAGGPILT PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRNDL 250 260 270 280 290 EISCDSQIAL PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein." Lipinska B., King J., Ang D., Georgopoulos C. Nucleic Acids Res. 16:7545-7562(1988) [PubMed: 3045760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-163. Strain: B178.
[5]	"Nucleotide sequence and LexA regulation of the Escherichia coli recN gene." Rostas K., Morton S.J., Picksley S.M., Lloyd R.G. Nucleic Acids Res. 15:5041-5049(1987) [PubMed: 3037486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-292. Strain: K12.
[6]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed: 7984428] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36840 Genomic DNA. Translation: AAA79785.1. U00096 Genomic DNA. Translation: AAC75664.1. AP009048 Genomic DNA. Translation: BAA16500.1. X07863 Genomic DNA. No translation available. Y00357 Genomic DNA. No translation available.
PIR	B65040.
RefSeq	AP_003195.1. NP_417105.1.
3D structure databases
SMR	P0A7B3. Positions 4-292.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A7B3.
Genome annotation databases
GeneID	947092.
GenomeReviews	Gene locus JW2596 in contig AP009048_GR. Gene locus b2615 in contig U00096_GR.
KEGG	ecj:JW2596. eco:b2615.
Organism-specific databases
EchoBASE	EB2109.
EcoGene	EG12192. ppnK.
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HBG713904.
OMA	MEQFRNI.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-541. ECOL168927:B2615-MONOMER. MetaCyc:MONOMER0-541.
Gene expression databases
Genevestigator	P0A7B3.
Family and domain databases
HAMAP	MF_00361. NAD_kinase. [Tree]
InterPro	IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. ATP_NADK. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPNK_ECOLI

Accession

Primary (citable) accession number: P0A7B3
Secondary accession number(s): P37768, P46140, P77490

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)