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P0A7B3 (PPNK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable inorganic polyphosphate/ATP-NAD kinase

Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23
Gene names
Name:ppnK
Synonyms:yfjB, yfjE
Ordered Locus Names:b2615, JW2596
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Sequence caution

The sequence X07863 differs from that shown. Reason: Frameshift at several positions.

The sequence Y00357 differs from that shown. Reason: Frameshift at positions 204, 215 and 282.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361
PRO_0000120616

Sequences

Sequence LengthMass (Da)Tools
P0A7B3 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: D1E631658408F2E1

FASTA29232,566
        10         20         30         40         50         60 
MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CTKGYEVIVE QQIAHELQLK NVKTGTLAEI 

        70         80         90        100        110        120 
GQLADLAVVV GGDGNMLGAA RTLARYDIKV IGINRGNLGF LTDLDPDNAQ QQLADVLEGH 

       130        140        150        160        170        180 
YISEKRFLLE AQVCQQDCQK RISTAINEVV LHPGKVAHMI EFEVYIDEIF AFSQRSDGLI 

       190        200        210        220        230        240 
ISTPTGSTAY SLSAGGPILT PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRNDL 

       250        260        270        280        290 
EISCDSQIAL PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF 

« Hide

References

« Hide 'large scale' references
[1]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein."
Lipinska B., King J., Ang D., Georgopoulos C.
Nucleic Acids Res. 16:7545-7562(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-163.
Strain: B178.
[5]"Nucleotide sequence and LexA regulation of the Escherichia coli recN gene."
Rostas K., Morton S.J., Picksley S.M., Lloyd R.G.
Nucleic Acids Res. 15:5041-5049(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-292.
Strain: K12.
[6]"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
Borodovsky M., Rudd K.E., Koonin E.V.
Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U36840 Genomic DNA. Translation: AAA79785.1.
U00096 Genomic DNA. Translation: AAC75664.1.
AP009048 Genomic DNA. Translation: BAA16500.1.
X07863 Genomic DNA. No translation available.
Y00357 Genomic DNA. No translation available.
PIRB65040.
RefSeqNP_417105.1. NC_000913.3.
YP_490837.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A7B3.
SMRP0A7B3. Positions 4-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid851428. 4 interactions.
DIPDIP-48103N.
IntActP0A7B3. 3 interactions.
STRING511145.b2615.

Proteomic databases

PaxDbP0A7B3.
PRIDEP0A7B3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75664; AAC75664; b2615.
BAA16500; BAA16500; BAA16500.
GeneID12931613.
947092.
KEGGecj:Y75_p2562.
eco:b2615.
PATRIC32120629. VBIEscCol129921_2713.

Organism-specific databases

EchoBASEEB2109.
EcoGeneEG12192. ppnK.

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227221.
KOK00858.
OMAGMVELRV.
OrthoDBEOG6PZXDR.
PhylomeDBP0A7B3.
ProtClustDBPRK03378.

Enzyme and pathway databases

BioCycEcoCyc:MONOMER0-541.
ECOL316407:JW2596-MONOMER.
MetaCyc:MONOMER0-541.

Gene expression databases

GenevestigatorP0A7B3.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK_prd.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Other

PROP0A7B3.

Entry information

Entry namePPNK_ECOLI
AccessionPrimary (citable) accession number: P0A7B3
Secondary accession number(s): P37768, P46140, P77490
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene