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Protein

Polyphosphate kinase

Gene

ppk

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP).UniRule annotation

Catalytic activityi

ATP + (phosphate)(n) = ADP + (phosphate)(n+1).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei435 – 4351Phosphohistidine intermediateUniRule annotation
Active sitei454 – 4541Phosphohistidine intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3325-MONOMER.
ECOO157:PPK-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphosphate kinaseUniRule annotation (EC:2.7.4.1UniRule annotation)
Alternative name(s):
ATP-polyphosphate phosphotransferaseUniRule annotation
Polyphosphoric acid kinaseUniRule annotation
Gene namesi
Name:ppkUniRule annotation
Ordered Locus Names:Z3764, ECs3363
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 688687Polyphosphate kinasePRO_0000128641Add
BLAST

Post-translational modificationi

An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to histidine residues through a N-P bond.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP0A7B2.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

MINTiMINT-1244096.
STRINGi155864.Z3764.

Structurei

3D structure databases

ProteinModelPortaliP0A7B2.
SMRiP0A7B2. Positions 2-688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini430 – 46435PLD phosphodiesteraseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyphosphate kinase family.UniRule annotation
Contains 1 PLD phosphodiesterase domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CUU. Bacteria.
COG0855. LUCA.
HOGENOMiHOG000248949.
KOiK00937.
OMAiLSSMIHA.
OrthoDBiEOG65TRRG.

Family and domain databases

Gene3Di3.30.1840.10. 1 hit.
HAMAPiMF_00347. Polyphosphate_kinase.
InterProiIPR001736. PLipase_D/transphosphatidylase.
IPR003414. PP_kinase.
IPR024953. PP_kinase_middle.
IPR025200. PPK_C_dom.
IPR025198. PPK_N_dom.
[Graphical view]
PfamiPF02503. PP_kinase. 1 hit.
PF13090. PP_kinase_C. 1 hit.
PF13089. PP_kinase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF015589. PP_kinase. 1 hit.
SUPFAMiSSF140356. SSF140356. 1 hit.
TIGRFAMsiTIGR03705. poly_P_kin. 1 hit.
PROSITEiPS50035. PLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQEKLYIEK ELSWLSFNER VLQEAADKSN PLIERMRFLG IYSNNLDEFY
60 70 80 90 100
KVRFAELKRR IIISEEQGSN SHSRHLLGKI QSRVLKADQE FDGLYNELLL
110 120 130 140 150
EMARNQIFLI NERQLSVNQQ NWLRHYFKQY LRQHITPILI NPDTDLVQFL
160 170 180 190 200
KDDYTYLAVE IIRGDTIRYA LLEIPSDKVP RFVNLPPEAP RRRKPMILLD
210 220 230 240 250
NILRYCLDDI FKGFFDYDAL NAYSMKMTRD AEYDLVHEME ASLMELMSSS
260 270 280 290 300
LKQRLTAEPV RFVYQRDMPN ALVEVLREKL TISRYDSIVP GGRYHNFKDF
310 320 330 340 350
INFPNVGKAN LVNKPLPRLR HIWFDKAQFR NGFDAIRERD VLLYYPYHTF
360 370 380 390 400
EHVLELLRQA SFDPSVLAIK INIYRVAKDS RIIDSMIHAA HNGKKVTVVV
410 420 430 440 450
ELQARFDEEA NIHWAKRLTE AGVHVIFSAP GLKIHAKLFL ISRKENGEVV
460 470 480 490 500
RYAHIGTGNF NEKTARLYTD YSLLTADARI TNEVRRVFNF IENPYRPVTF
510 520 530 540 550
DYLMVSPQNS RRLLYEMVDR EIANAQQGLP SGITLKLNNL VDKGLVDRLY
560 570 580 590 600
AASSSGVPVN LLVRGMCSLI PNLEGISDNI RAISIVDRYL EHDRVYIFEN
610 620 630 640 650
GGDKKVYLSS ADWMTRNIDY RIEVATPLLD PRLKQRVLDI IDILFSDTVK
660 670 680
ARYIDKELSN RYVPRGNRRK VRAQLAIYDY IKSLEQPE
Length:688
Mass (Da):80,432
Last modified:January 23, 2007 - v2
Checksum:iE1EA6C53432E6935
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57611.1.
BA000007 Genomic DNA. Translation: BAB36786.1.
PIRiC91049.
G85893.
RefSeqiNP_311390.1. NC_002695.1.
WP_000529576.1. NZ_LPWC01000179.1.

Genome annotation databases

EnsemblBacteriaiAAG57611; AAG57611; Z3764.
BAB36786; BAB36786; BAB36786.
GeneIDi912733.
KEGGiece:Z3764.
ecs:ECs3363.
PATRICi18356148. VBIEscCol44059_3269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57611.1.
BA000007 Genomic DNA. Translation: BAB36786.1.
PIRiC91049.
G85893.
RefSeqiNP_311390.1. NC_002695.1.
WP_000529576.1. NZ_LPWC01000179.1.

3D structure databases

ProteinModelPortaliP0A7B2.
SMRiP0A7B2. Positions 2-688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1244096.
STRINGi155864.Z3764.

Proteomic databases

PRIDEiP0A7B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57611; AAG57611; Z3764.
BAB36786; BAB36786; BAB36786.
GeneIDi912733.
KEGGiece:Z3764.
ecs:ECs3363.
PATRICi18356148. VBIEscCol44059_3269.

Phylogenomic databases

eggNOGiENOG4105CUU. Bacteria.
COG0855. LUCA.
HOGENOMiHOG000248949.
KOiK00937.
OMAiLSSMIHA.
OrthoDBiEOG65TRRG.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3325-MONOMER.
ECOO157:PPK-MONOMER.

Family and domain databases

Gene3Di3.30.1840.10. 1 hit.
HAMAPiMF_00347. Polyphosphate_kinase.
InterProiIPR001736. PLipase_D/transphosphatidylase.
IPR003414. PP_kinase.
IPR024953. PP_kinase_middle.
IPR025200. PPK_C_dom.
IPR025198. PPK_N_dom.
[Graphical view]
PfamiPF02503. PP_kinase. 1 hit.
PF13090. PP_kinase_C. 1 hit.
PF13089. PP_kinase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF015589. PP_kinase. 1 hit.
SUPFAMiSSF140356. SSF140356. 1 hit.
TIGRFAMsiTIGR03705. poly_P_kin. 1 hit.
PROSITEiPS50035. PLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPPK_ECO57
AccessioniPrimary (citable) accession number: P0A7B2
Secondary accession number(s): P28688, Q47549
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.