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P0A7B2 (PPK_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyphosphate kinase
EC=2.7.4.1
Alternative name(s):
ATP-polyphosphate phosphotransferase
Polyphosphoric acid kinase
Gene names
Name:ppk
Ordered Locus Names:Z3764, ECs3363
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length688 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP) By similarity. HAMAP-Rule MF_00347

Catalytic activity

ATP + (phosphate)(n) = ADP + (phosphate)(n+1). HAMAP-Rule MF_00347

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00347

Subcellular location

Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00347.

Post-translational modification

An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to histidine residues through a N-P bond By similarity. HAMAP-Rule MF_00347

Sequence similarities

Belongs to the polyphosphate kinase family.

Contains 1 PLD phosphodiesterase domain.

Ontologies

Keywords
   Cellular componentCell inner membrane
Cell membrane
Membrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpolyphosphate biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

polyphosphate kinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyphosphate kinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 688687Polyphosphate kinase HAMAP-Rule MF_00347
PRO_0000128641

Regions

Domain430 – 46435PLD phosphodiesterase

Sites

Active site4351Phosphohistidine intermediate By similarity
Active site4541Phosphohistidine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A7B2 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: E1EA6C53432E6935

FASTA68880,432
        10         20         30         40         50         60 
MGQEKLYIEK ELSWLSFNER VLQEAADKSN PLIERMRFLG IYSNNLDEFY KVRFAELKRR 

        70         80         90        100        110        120 
IIISEEQGSN SHSRHLLGKI QSRVLKADQE FDGLYNELLL EMARNQIFLI NERQLSVNQQ 

       130        140        150        160        170        180 
NWLRHYFKQY LRQHITPILI NPDTDLVQFL KDDYTYLAVE IIRGDTIRYA LLEIPSDKVP 

       190        200        210        220        230        240 
RFVNLPPEAP RRRKPMILLD NILRYCLDDI FKGFFDYDAL NAYSMKMTRD AEYDLVHEME 

       250        260        270        280        290        300 
ASLMELMSSS LKQRLTAEPV RFVYQRDMPN ALVEVLREKL TISRYDSIVP GGRYHNFKDF 

       310        320        330        340        350        360 
INFPNVGKAN LVNKPLPRLR HIWFDKAQFR NGFDAIRERD VLLYYPYHTF EHVLELLRQA 

       370        380        390        400        410        420 
SFDPSVLAIK INIYRVAKDS RIIDSMIHAA HNGKKVTVVV ELQARFDEEA NIHWAKRLTE 

       430        440        450        460        470        480 
AGVHVIFSAP GLKIHAKLFL ISRKENGEVV RYAHIGTGNF NEKTARLYTD YSLLTADARI 

       490        500        510        520        530        540 
TNEVRRVFNF IENPYRPVTF DYLMVSPQNS RRLLYEMVDR EIANAQQGLP SGITLKLNNL 

       550        560        570        580        590        600 
VDKGLVDRLY AASSSGVPVN LLVRGMCSLI PNLEGISDNI RAISIVDRYL EHDRVYIFEN 

       610        620        630        640        650        660 
GGDKKVYLSS ADWMTRNIDY RIEVATPLLD PRLKQRVLDI IDILFSDTVK ARYIDKELSN 

       670        680 
RYVPRGNRRK VRAQLAIYDY IKSLEQPE

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG57611.1.
BA000007 Genomic DNA. Translation: BAB36786.1.
PIRC91049.
G85893.
RefSeqNP_289054.1. NC_002655.2.
NP_311390.1. NC_002695.1.

3D structure databases

ProteinModelPortalP0A7B2.
SMRP0A7B2. Positions 2-688.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-1244096.
STRING155864.Z3764.

Proteomic databases

PRIDEP0A7B2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG57611; AAG57611; Z3764.
BAB36786; BAB36786; BAB36786.
GeneID912733.
957762.
KEGGece:Z3764.
ecs:ECs3363.
PATRIC18356148. VBIEscCol44059_3269.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0855.
HOGENOMHOG000248949.
KOK00937.
OMAILLYYPY.
ProtClustDBPRK05443.

Enzyme and pathway databases

BioCycECOL386585:GJFA-3325-MONOMER.

Family and domain databases

Gene3D3.30.1840.10. 1 hit.
HAMAPMF_00347. Polyphosphate_kinase.
InterProIPR001736. PLipase_D/transphosphatidylase.
IPR003414. PP_kinase.
IPR024953. PP_kinase_middle.
IPR025200. PPK_C_dom.
IPR025198. PPK_N_dom.
[Graphical view]
PfamPF02503. PP_kinase. 1 hit.
PF13090. PP_kinase_C. 1 hit.
PF13089. PP_kinase_N. 1 hit.
[Graphical view]
PIRSFPIRSF015589. PP_kinase. 1 hit.
SUPFAMSSF140356. SSF140356. 1 hit.
TIGRFAMsTIGR03705. poly_P_kin. 1 hit.
PROSITEPS50035. PLD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPK_ECO57
AccessionPrimary (citable) accession number: P0A7B2
Secondary accession number(s): P28688, Q47549
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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