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Protein

Polyphosphate kinase

Gene

ppk

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP).UniRule annotation

Catalytic activityi

ATP + (phosphate)(n) = ADP + (phosphate)(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATPUniRule annotation1
Metal bindingi375MagnesiumUniRule annotation1
Metal bindingi405MagnesiumUniRule annotation1
Active sitei435Phosphohistidine intermediateUniRule annotation1
Binding sitei468ATPUniRule annotation1
Binding sitei564ATPUniRule annotation1
Binding sitei592ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphosphate kinaseUniRule annotation (EC:2.7.4.1UniRule annotation)
Alternative name(s):
ATP-polyphosphate phosphotransferaseUniRule annotation
Polyphosphoric acid kinaseUniRule annotation
Gene namesi
Name:ppkUniRule annotation
Ordered Locus Names:Z3764, ECs3363
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001286412 – 688Polyphosphate kinaseAdd BLAST687

Post-translational modificationi

An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to a histidine residue through a N-P bond.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP0A7B2

Interactioni

Protein-protein interaction databases

STRINGi155864.Z3764

Structurei

3D structure databases

ProteinModelPortaliP0A7B2
SMRiP0A7B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini430 – 464PLD phosphodiesteraseUniRule annotationAdd BLAST35

Sequence similaritiesi

Belongs to the polyphosphate kinase 1 (PPK1) family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CUU Bacteria
COG0855 LUCA
HOGENOMiHOG000248949
KOiK00937
OMAiQIMVANL

Family and domain databases

Gene3Di3.30.1840.10, 1 hit
HAMAPiMF_00347 Polyphosphate_kinase, 1 hit
InterProiView protein in InterPro
IPR001736 PLipase_D/transphosphatidylase
IPR003414 PP_kinase
IPR024953 PP_kinase_middle
IPR036830 PP_kinase_middle_dom_sf
IPR025200 PPK_C_dom
IPR025198 PPK_N_dom
IPR036832 PPK_N_dom_sf
PANTHERiPTHR30218 PTHR30218, 1 hit
PfamiView protein in Pfam
PF02503 PP_kinase, 1 hit
PF13090 PP_kinase_C, 1 hit
PF13089 PP_kinase_N, 1 hit
PIRSFiPIRSF015589 PP_kinase, 1 hit
SUPFAMiSSF140356 SSF140356, 1 hit
TIGRFAMsiTIGR03705 poly_P_kin, 1 hit
PROSITEiView protein in PROSITE
PS50035 PLD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQEKLYIEK ELSWLSFNER VLQEAADKSN PLIERMRFLG IYSNNLDEFY
60 70 80 90 100
KVRFAELKRR IIISEEQGSN SHSRHLLGKI QSRVLKADQE FDGLYNELLL
110 120 130 140 150
EMARNQIFLI NERQLSVNQQ NWLRHYFKQY LRQHITPILI NPDTDLVQFL
160 170 180 190 200
KDDYTYLAVE IIRGDTIRYA LLEIPSDKVP RFVNLPPEAP RRRKPMILLD
210 220 230 240 250
NILRYCLDDI FKGFFDYDAL NAYSMKMTRD AEYDLVHEME ASLMELMSSS
260 270 280 290 300
LKQRLTAEPV RFVYQRDMPN ALVEVLREKL TISRYDSIVP GGRYHNFKDF
310 320 330 340 350
INFPNVGKAN LVNKPLPRLR HIWFDKAQFR NGFDAIRERD VLLYYPYHTF
360 370 380 390 400
EHVLELLRQA SFDPSVLAIK INIYRVAKDS RIIDSMIHAA HNGKKVTVVV
410 420 430 440 450
ELQARFDEEA NIHWAKRLTE AGVHVIFSAP GLKIHAKLFL ISRKENGEVV
460 470 480 490 500
RYAHIGTGNF NEKTARLYTD YSLLTADARI TNEVRRVFNF IENPYRPVTF
510 520 530 540 550
DYLMVSPQNS RRLLYEMVDR EIANAQQGLP SGITLKLNNL VDKGLVDRLY
560 570 580 590 600
AASSSGVPVN LLVRGMCSLI PNLEGISDNI RAISIVDRYL EHDRVYIFEN
610 620 630 640 650
GGDKKVYLSS ADWMTRNIDY RIEVATPLLD PRLKQRVLDI IDILFSDTVK
660 670 680
ARYIDKELSN RYVPRGNRRK VRAQLAIYDY IKSLEQPE
Length:688
Mass (Da):80,432
Last modified:January 23, 2007 - v2
Checksum:iE1EA6C53432E6935
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG57611.1
BA000007 Genomic DNA Translation: BAB36786.1
PIRiC91049
G85893
RefSeqiNP_311390.1, NC_002695.1
WP_000529576.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG57611; AAG57611; Z3764
BAB36786; BAB36786; BAB36786
GeneIDi912733
KEGGiece:Z3764
ecs:ECs3363
PATRICifig|386585.9.peg.3513

Similar proteinsi

Entry informationi

Entry nameiPPK1_ECO57
AccessioniPrimary (citable) accession number: P0A7B2
Secondary accession number(s): P28688, Q47549
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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