Reviewed, UniProtKB/Swiss-Prot P0A7B1 (PPK_ECOLI)

Names and origin

Protein names

Recommended name:
    Polyphosphate kinase
    EC=2.7.4.1
Alternative name(s):
    Polyphosphoric acid kinase
    ATP-polyphosphate phosphotransferase

Gene names

Name:	ppk
Ordered Locus Names:	b2501, JW2486

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can also act in the reverse direction to form ATP in the presence of excess ADP. Can also use GTP instead of ATP; but the efficiency of GTP is 5% that of ATP. HAMAP MF_00347
Catalytic activity	ATP + (phosphate)(n) = ADP + (phosphate)(n+1). HAMAP MF_00347
Subunit structure	Homotetramer. HAMAP MF_00347
Subcellular location	Cell inner membrane; Peripheral membrane protein. HAMAP MF_00347
Post-translational modification	An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to histidine residues through a N-P bond. HAMAP MF_00347
Sequence similarities	Belongs to the polyphosphate kinase family. Contains 1 PLD phosphodiesterase domain.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	polyphosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell polyphosphate kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polyphosphate kinase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yafC	P30864	1	EBI-544152,EBI-548591
yfbV	P0A8D9	1	EBI-544152,EBI-1128326

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed HAMAP MF_00347

Chain

2 – 688

687

Polyphosphate kinase HAMAP MF_00347

PRO_0000128640

Regions

Domain

430 – 464

35

PLD phosphodiesterase

Sites

Active site

435

1

Phosphohistidine intermediate HAMAP MF_00347

Active site

454

1

Phosphohistidine intermediate HAMAP MF_00347

Secondary structure

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688

Helix Strand Turn

Details...

Helix

10 – 26

17

Helix

32 – 51

20

Helix

53 – 65

13

Helix

73 – 104

32

Beta strand

107 – 110

4

Helix

112 – 114

3

Helix

119 – 130

12

Helix

132 – 134

3

Turn

146 – 149

4

Beta strand

155 – 163

9

Beta strand

166 – 173

8

Turn

176 – 178

3

Beta strand

181 – 184

4

Beta strand

194 – 198

5

Helix

199 – 211

13

Turn

212 – 214

3

Beta strand

218 – 229

12

Beta strand

237 – 239

3

Helix

242 – 246

5

Helix

249 – 256

8

Beta strand

260 – 264

5

Helix

270 – 279

10

Beta strand

287 – 290

4

Helix

297 – 302

6

Helix

309 – 311

3

Helix

322 – 325

4

Helix

332 – 338

7

Beta strand

341 – 344

4

Turn

345 – 347

3

Helix

351 – 360

10

Beta strand

366 – 375

10

Helix

381 – 391

11

Beta strand

395 – 400

6

Helix

409 – 411

3

Helix

416 – 420

5

Beta strand

424 – 427

4

Beta strand

437 – 445

9

Beta strand

448 – 458

11

Helix

464 – 467

4

Beta strand

471 – 475

5

Helix

478 – 492

15

Beta strand

504 – 506

3

Helix

511 – 525

15

Turn

526 – 528

3

Beta strand

533 – 538

6

Helix

543 – 554

12

Beta strand

559 – 565

7

Turn

574 – 576

3

Beta strand

580 – 585

6

Beta strand

588 – 591

4

Beta strand

594 – 598

5

Beta strand

605 – 610

6

Helix

615 – 619

5

Beta strand

621 – 627

7

Helix

631 – 645

15

Beta strand

648 – 653

6

Helix

673 – 684

12

Sequences

	Sequence		Length	Mass (Da)
	P0A7B1-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E1EA6C53432E6935	FASTA	688	80,432
	10 20 30 40 50 60 MGQEKLYIEK ELSWLSFNER VLQEAADKSN PLIERMRFLG IYSNNLDEFY KVRFAELKRR 70 80 90 100 110 120 IIISEEQGSN SHSRHLLGKI QSRVLKADQE FDGLYNELLL EMARNQIFLI NERQLSVNQQ 130 140 150 160 170 180 NWLRHYFKQY LRQHITPILI NPDTDLVQFL KDDYTYLAVE IIRGDTIRYA LLEIPSDKVP 190 200 210 220 230 240 RFVNLPPEAP RRRKPMILLD NILRYCLDDI FKGFFDYDAL NAYSMKMTRD AEYDLVHEME 250 260 270 280 290 300 ASLMELMSSS LKQRLTAEPV RFVYQRDMPN ALVEVLREKL TISRYDSIVP GGRYHNFKDF 310 320 330 340 350 360 INFPNVGKAN LVNKPLPRLR HIWFDKAQFR NGFDAIRERD VLLYYPYHTF EHVLELLRQA 370 380 390 400 410 420 SFDPSVLAIK INIYRVAKDS RIIDSMIHAA HNGKKVTVVV ELQARFDEEA NIHWAKRLTE 430 440 450 460 470 480 AGVHVIFSAP GLKIHAKLFL ISRKENGEVV RYAHIGTGNF NEKTARLYTD YSLLTADARI 490 500 510 520 530 540 TNEVRRVFNF IENPYRPVTF DYLMVSPQNS RRLLYEMVDR EIANAQQGLP SGITLKLNNL 550 560 570 580 590 600 VDKGLVDRLY AASSSGVPVN LLVRGMCSLI PNLEGISDNI RAISIVDRYL EHDRVYIFEN 610 620 630 640 650 660 GGDKKVYLSS ADWMTRNIDY RIEVATPLLD PRLKQRVLDI IDILFSDTVK ARYIDKELSN 670 680 RYVPRGNRRK VRAQLAIYDY IKSLEQPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." Akiyama M., Crooke E., Kornberg A. J. Biol. Chem. 267:22556-22561(1992) [PubMed: 1331061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12." Smith J.M., Daum H.A. III J. Biol. Chem. 262:10565-10569(1987) [PubMed: 3301838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-91. Strain: K12.
[6]	"An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon." Akiyama M., Crooke E., Kornberg A. J. Biol. Chem. 268:633-639(1993) [PubMed: 8380170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 679-688. Strain: K12.
[7]	"Phosphohistidyl active sites in polyphosphate kinase of Escherichia coli." Kumble K.D., Ahn K., Kornberg A. Proc. Natl. Acad. Sci. U.S.A. 93:14391-14395(1996) [PubMed: 8962061] [Abstract] Cited for: PROTEIN SEQUENCE OF 434-437, MUTAGENESIS OF HISTIDINE RESIDUES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L03719 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75554.1.
AP009048 Genomic DNA. Translation: BAA16389.1.
M13747 Genomic DNA. Translation: AAA83900.1. Different initiation.
L06129 Genomic DNA. No translation available.

PIR

A44306.

RefSeq

AP_003087.1.
NP_416996.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XDO	X-ray	3.00	A/B	2-687	[»]
1XDP	X-ray	2.50	A/B	2-687	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A7B1. 23 interactions.

Genome annotation databases

GeneID

946971.

GenomeReviews

Gene locus JW2486 in contig AP009048_GR.
Gene locus b2501 in contig U00096_GR.

KEGG

ecj:JW2486.
eco:b2501.

Organism-specific databases

EchoBASE

EB1472.

EcoGene

EG11510. ppk.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A7B1.

OMA

P0A7B1. DEFFRVR.

Enzyme and pathway databases

BioCyc

EcoCyc:PPK-MON.
MetaCyc:PPK-MON.

Family and domain databases

HAMAP

MF_00347.
[Tree]

InterPro

IPR001736. PLipase_D/transphosphatidylase.
IPR003414. PP_kinase.
[Graphical view]

Pfam

PF02503. PP_kinase. 1 hit.
[Graphical view]

PIRSF

PIRSF015589. PP_kinase. 1 hit.

PROSITE

PS50035. PLD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PPK_ECOLI

Accession

Primary (citable) accession number: P0A7B1
Secondary accession number(s): P28688, Q47549

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)