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Protein

Inorganic pyrophosphatase

Gene

ppa

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30SubstrateUniRule annotation1
Binding sitei44SubstrateUniRule annotation1
Binding sitei56SubstrateUniRule annotation1
Metal bindingi66Magnesium 1UniRule annotation1
Metal bindingi71Magnesium 1UniRule annotation1
Metal bindingi71Magnesium 2UniRule annotation1
Metal bindingi103Magnesium 1UniRule annotation1
Binding sitei142SubstrateUniRule annotation1

GO - Molecular functioni

  • inorganic diphosphatase activity Source: EcoCyc
  • magnesium ion binding Source: EcoCyc
  • triphosphatase activity Source: EcoCyc
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:INORGPYROPHOSPHAT-MONOMER.
ECOL316407:JW4185-MONOMER.
MetaCyc:INORGPYROPHOSPHAT-MONOMER.
BRENDAi3.6.1.1. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphataseUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Pyrophosphate phospho-hydrolaseUniRule annotation
Short name:
PPaseUniRule annotation
Gene namesi
Name:ppaUniRule annotation
Ordered Locus Names:b4226, JW4185
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10755. ppa.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21E → D: 16% activity. 1
Mutagenesisi30K → R: 2% activity. 1
Mutagenesisi32E → D: 6% activity. 1
Mutagenesisi44R → K: 10% activity. 1
Mutagenesisi52Y → F: 64% activity. 1
Mutagenesisi56Y → F: 7% activity. 1
Mutagenesisi66D → E: 6% activity. 1
Mutagenesisi68D → E: 1% activity. 1
Mutagenesisi71D → E: No activity. 1
Mutagenesisi98D → E: 22% activity. 1
Mutagenesisi98D → V: No activity. 1
Mutagenesisi99E → V: 33% activity. 1
Mutagenesisi103D → E: 3% activity. 1
Mutagenesisi103D → V: No activity. 1
Mutagenesisi105K → I: No activity. 1
Mutagenesisi105K → R: 3% activity. 1
Mutagenesisi142Y → F: 22% activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001374952 – 176Inorganic pyrophosphataseAdd BLAST175

Proteomic databases

EPDiP0A7A9.
PaxDbiP0A7A9.
PRIDEiP0A7A9.

2D gel databases

SWISS-2DPAGEP0A7A9.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

BioGridi4259312. 36 interactors.
DIPiDIP-36217N.
IntActiP0A7A9. 36 interactors.
MINTiMINT-1226708.
STRINGi511145.b4226.

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Beta strandi8 – 11Combined sources4
Turni12 – 14Combined sources3
Beta strandi15 – 22Combined sources8
Beta strandi28 – 33Combined sources6
Turni35 – 37Combined sources3
Beta strandi40 – 45Combined sources6
Beta strandi47 – 49Combined sources3
Beta strandi53 – 58Combined sources6
Beta strandi65 – 68Combined sources4
Beta strandi71 – 74Combined sources4
Beta strandi85 – 98Combined sources14
Beta strandi99 – 101Combined sources3
Beta strandi105 – 110Combined sources6
Turni112 – 114Combined sources3
Turni117 – 120Combined sources4
Helixi124 – 126Combined sources3
Helixi129 – 141Combined sources13
Turni142 – 145Combined sources4
Beta strandi151 – 157Combined sources7
Helixi159 – 175Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FAJX-ray2.15A2-176[»]
1I40X-ray1.10A2-176[»]
1I6TX-ray1.20A2-176[»]
1IGPX-ray2.20A2-176[»]
1INOX-ray2.20A2-176[»]
1IPWX-ray2.30A/B2-176[»]
1JFDX-ray2.20A/B2-176[»]
1MJWX-ray1.95A/B2-176[»]
1MJXX-ray2.15A/B2-176[»]
1MJYX-ray2.10A/B2-176[»]
1MJZX-ray2.20A2-176[»]
1OBWX-ray1.90A/B/C2-176[»]
2AU6X-ray1.20A2-176[»]
2AU7X-ray1.05A2-176[»]
2AU8X-ray1.65A2-176[»]
2AU9X-ray1.30A2-176[»]
2AUUX-ray1.22A2-176[»]
2EIPX-ray2.20A/B2-176[»]
4UM4X-ray2.65A/B/C1-176[»]
ProteinModelPortaliP0A7A9.
SMRiP0A7A9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7A9.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F0N. Bacteria.
COG0221. LUCA.
HOGENOMiHOG000236473.
InParanoidiP0A7A9.
KOiK01507.
OMAiPVDYGII.
PhylomeDBiP0A7A9.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM
60 70 80 90 100
FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA
110 120 130 140 150
GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW
160 170
VKVEGWENAE AAKAEIVASF ERAKNK
Length:176
Mass (Da):19,704
Last modified:January 23, 2007 - v2
Checksum:i4C97E51F3BE650DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23550 Unassigned DNA. Translation: AAB88583.1.
U14003 Genomic DNA. Translation: AAA97123.1.
U00096 Genomic DNA. Translation: AAC77183.1.
AP009048 Genomic DNA. Translation: BAE78227.1.
PIRiA27648. PWEC.
RefSeqiNP_418647.1. NC_000913.3.
WP_000055075.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77183; AAC77183; b4226.
BAE78227; BAE78227; BAE78227.
GeneIDi948748.
KEGGiecj:JW4185.
eco:b4226.
PATRICi32124027. VBIEscCol129921_4358.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23550 Unassigned DNA. Translation: AAB88583.1.
U14003 Genomic DNA. Translation: AAA97123.1.
U00096 Genomic DNA. Translation: AAC77183.1.
AP009048 Genomic DNA. Translation: BAE78227.1.
PIRiA27648. PWEC.
RefSeqiNP_418647.1. NC_000913.3.
WP_000055075.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FAJX-ray2.15A2-176[»]
1I40X-ray1.10A2-176[»]
1I6TX-ray1.20A2-176[»]
1IGPX-ray2.20A2-176[»]
1INOX-ray2.20A2-176[»]
1IPWX-ray2.30A/B2-176[»]
1JFDX-ray2.20A/B2-176[»]
1MJWX-ray1.95A/B2-176[»]
1MJXX-ray2.15A/B2-176[»]
1MJYX-ray2.10A/B2-176[»]
1MJZX-ray2.20A2-176[»]
1OBWX-ray1.90A/B/C2-176[»]
2AU6X-ray1.20A2-176[»]
2AU7X-ray1.05A2-176[»]
2AU8X-ray1.65A2-176[»]
2AU9X-ray1.30A2-176[»]
2AUUX-ray1.22A2-176[»]
2EIPX-ray2.20A/B2-176[»]
4UM4X-ray2.65A/B/C1-176[»]
ProteinModelPortaliP0A7A9.
SMRiP0A7A9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259312. 36 interactors.
DIPiDIP-36217N.
IntActiP0A7A9. 36 interactors.
MINTiMINT-1226708.
STRINGi511145.b4226.

2D gel databases

SWISS-2DPAGEP0A7A9.

Proteomic databases

EPDiP0A7A9.
PaxDbiP0A7A9.
PRIDEiP0A7A9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77183; AAC77183; b4226.
BAE78227; BAE78227; BAE78227.
GeneIDi948748.
KEGGiecj:JW4185.
eco:b4226.
PATRICi32124027. VBIEscCol129921_4358.

Organism-specific databases

EchoBASEiEB0748.
EcoGeneiEG10755. ppa.

Phylogenomic databases

eggNOGiENOG4105F0N. Bacteria.
COG0221. LUCA.
HOGENOMiHOG000236473.
InParanoidiP0A7A9.
KOiK01507.
OMAiPVDYGII.
PhylomeDBiP0A7A9.

Enzyme and pathway databases

BioCyciEcoCyc:INORGPYROPHOSPHAT-MONOMER.
ECOL316407:JW4185-MONOMER.
MetaCyc:INORGPYROPHOSPHAT-MONOMER.
BRENDAi3.6.1.1. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A7A9.
PROiP0A7A9.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPYR_ECOLI
AccessioniPrimary (citable) accession number: P0A7A9
Secondary accession number(s): P17288, Q2M679
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.