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Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. This enzyme does not act on D-aspartyl residues.

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei59By similarity1

GO - Molecular functioni

  • protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10689-MONOMER.
ECOL316407:JW2713-MONOMER.
MetaCyc:EG10689-MONOMER.
BRENDAi2.1.1.77. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate O-methyltransferase (EC:2.1.1.77)
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name:
PIMT
Gene namesi
Name:pcm
Ordered Locus Names:b2743, JW2713
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10689. pcm.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001118862 – 208Protein-L-isoaspartate O-methyltransferaseAdd BLAST207

Proteomic databases

PaxDbiP0A7A5.
PRIDEiP0A7A5.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4263145. 10 interactors.
IntActiP0A7A5. 2 interactors.
STRINGi511145.b2743.

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 15Combined sources12
Helixi21 – 29Combined sources9
Helixi32 – 35Combined sources4
Helixi38 – 43Combined sources6
Beta strandi46 – 48Combined sources3
Helixi61 – 70Combined sources10
Beta strandi78 – 82Combined sources5
Helixi88 – 96Combined sources9
Beta strandi97 – 106Combined sources10
Helixi107 – 119Combined sources13
Beta strandi124 – 130Combined sources7
Helixi132 – 134Combined sources3
Helixi137 – 139Combined sources3
Beta strandi142 – 150Combined sources9
Helixi157 – 160Combined sources4
Beta strandi162 – 172Combined sources11
Beta strandi174 – 176Combined sources3
Beta strandi178 – 185Combined sources8
Beta strandi188 – 196Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LBFX-ray1.80A/B/C/D1-208[»]
ProteinModelPortaliP0A7A5.
SMRiP0A7A5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A7A5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105E26. Bacteria.
COG2518. LUCA.
HOGENOMiHOG000257189.
InParanoidiP0A7A5.
KOiK00573.
OMAiPYTVAFQ.
PhylomeDBiP0A7A5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A7A5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRRVQALL DQLRAQGIQD EQVLNALAAV PREKFVDEAF EQKAWDNIAL
60 70 80 90 100
PIGQGQTISQ PYMVARMTEL LELTPQSRVL EIGTGSGYQT AILAHLVQHV
110 120 130 140 150
CSVERIKGLQ WQARRRLKNL DLHNVSTRHG DGWQGWQARA PFDAIIVTAA
160 170 180 190 200
PPEIPTALMT QLDEGGILVL PVGEEHQYLK RVRRRGGEFI IDTVEAVRFV

PLVKGELA
Length:208
Mass (Da):23,258
Last modified:January 23, 2007 - v2
Checksum:iBFA179567527A118
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63493 Genomic DNA. Translation: AAA24302.1.
U29579 Genomic DNA. Translation: AAA69253.1.
U00096 Genomic DNA. Translation: AAC75785.1.
AP009048 Genomic DNA. Translation: BAE76820.1.
L07942 Genomic DNA. Translation: AAA79840.1.
L07869 Unassigned DNA. Translation: AAA17874.1.
D17549 Genomic DNA. Translation: BAA04486.1.
PIRiJH0242.
RefSeqiNP_417223.1. NC_000913.3.
WP_000254708.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75785; AAC75785; b2743.
BAE76820; BAE76820; BAE76820.
GeneIDi944923.
KEGGiecj:JW2713.
eco:b2743.
PATRICi32120892. VBIEscCol129921_2838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63493 Genomic DNA. Translation: AAA24302.1.
U29579 Genomic DNA. Translation: AAA69253.1.
U00096 Genomic DNA. Translation: AAC75785.1.
AP009048 Genomic DNA. Translation: BAE76820.1.
L07942 Genomic DNA. Translation: AAA79840.1.
L07869 Unassigned DNA. Translation: AAA17874.1.
D17549 Genomic DNA. Translation: BAA04486.1.
PIRiJH0242.
RefSeqiNP_417223.1. NC_000913.3.
WP_000254708.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LBFX-ray1.80A/B/C/D1-208[»]
ProteinModelPortaliP0A7A5.
SMRiP0A7A5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263145. 10 interactors.
IntActiP0A7A5. 2 interactors.
STRINGi511145.b2743.

Proteomic databases

PaxDbiP0A7A5.
PRIDEiP0A7A5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75785; AAC75785; b2743.
BAE76820; BAE76820; BAE76820.
GeneIDi944923.
KEGGiecj:JW2713.
eco:b2743.
PATRICi32120892. VBIEscCol129921_2838.

Organism-specific databases

EchoBASEiEB0683.
EcoGeneiEG10689. pcm.

Phylogenomic databases

eggNOGiENOG4105E26. Bacteria.
COG2518. LUCA.
HOGENOMiHOG000257189.
InParanoidiP0A7A5.
KOiK00573.
OMAiPYTVAFQ.
PhylomeDBiP0A7A5.

Enzyme and pathway databases

BioCyciEcoCyc:EG10689-MONOMER.
ECOL316407:JW2713-MONOMER.
MetaCyc:EG10689-MONOMER.
BRENDAi2.1.1.77. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A7A5.
PROiP0A7A5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMT_ECOLI
AccessioniPrimary (citable) accession number: P0A7A5
Secondary accession number(s): P24206, Q2MA86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.