Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0A799

- PGK_ECOLI

UniProt

P0A799 - PGK_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei146 – 1461SubstrateBy similarity
Binding sitei197 – 1971ATPBy similarity
Binding sitei314 – 3141ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi340 – 3434ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoglycerate kinase activity Source: EcoCyc

GO - Biological processi

  1. glycolytic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PGK.
ECOL316407:JW2893-MONOMER.
MetaCyc:PGK.
SABIO-RKP0A799.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:b2926, JW2893
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10703. pgk.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 387386Phosphoglycerate kinasePRO_0000145941Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 841N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP0A799.
PRIDEiP0A799.

2D gel databases

SWISS-2DPAGEP0A799.

PTM databases

PhosSiteiP0810419.

Expressioni

Gene expression databases

GenevestigatoriP0A799.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-36163N.
IntActiP0A799. 15 interactions.
MINTiMINT-1229247.
STRINGi511145.b2926.

Structurei

Secondary structure

1
387
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 83Combined sources
Beta strandi15 – 195Combined sources
Beta strandi30 – 323Combined sources
Helixi35 – 4915Combined sources
Beta strandi53 – 575Combined sources
Helixi70 – 723Combined sources
Helixi75 – 8410Combined sources
Beta strandi89 – 935Combined sources
Beta strandi106 – 1094Combined sources
Helixi112 – 1143Combined sources
Turni116 – 1216Combined sources
Helixi123 – 1319Combined sources
Beta strandi134 – 1385Combined sources
Helixi141 – 1433Combined sources
Turni149 – 1535Combined sources
Helixi154 – 1574Combined sources
Beta strandi158 – 1636Combined sources
Helixi165 – 17814Combined sources
Beta strandi182 – 19211Combined sources
Turni194 – 1974Combined sources
Helixi198 – 2058Combined sources
Beta strandi209 – 2157Combined sources
Helixi216 – 2249Combined sources
Helixi236 – 2383Combined sources
Helixi239 – 2468Combined sources
Beta strandi255 – 26612Combined sources
Beta strandi270 – 2734Combined sources
Helixi274 – 2763Combined sources
Beta strandi282 – 2865Combined sources
Helixi288 – 30013Combined sources
Beta strandi302 – 3087Combined sources
Helixi316 – 3183Combined sources
Helixi320 – 33112Combined sources
Beta strandi332 – 3387Combined sources
Helixi341 – 35010Combined sources
Helixi353 – 3553Combined sources
Beta strandi356 – 3594Combined sources
Helixi364 – 3707Combined sources
Helixi376 – 3849Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZMRX-ray2.40A1-387[»]
ProteinModelPortaliP0A799.
SMRiP0A799. Positions 2-387.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A799.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 233Substrate bindingBy similarity
Regioni59 – 624Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227107.
InParanoidiP0A799.
KOiK00927.
OMAiAGHPVGK.
OrthoDBiEOG64N9Z0.
PhylomeDBiP0A799.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A799-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVIKMTDLD LAGKRVFIRA DLNVPVKDGK VTSDARIRAS LPTIELALKQ
60 70 80 90 100
GAKVMVTSHL GRPTEGEYNE EFSLLPVVNY LKDKLSNPVR LVKDYLDGVD
110 120 130 140 150
VAEGELVVLE NVRFNKGEKK DDETLSKKYA ALCDVFVMDA FGTAHRAQAS
160 170 180 190 200
THGIGKFADV ACAGPLLAAE LDALGKALKE PARPMVAIVG GSKVSTKLTV
210 220 230 240 250
LDSLSKIADQ LIVGGGIANT FIAAQGHDVG KSLYEADLVD EAKRLLTTCN
260 270 280 290 300
IPVPSDVRVA TEFSETAPAT LKSVNDVKAD EQILDIGDAS AQELAEILKN
310 320 330 340 350
AKTILWNGPV GVFEFPNFRK GTEIVANAIA DSEAFSIAGG GDTLAAIDLF
360 370 380
GIADKISYIS TGGGAFLEFV EGKVLPAVAM LEERAKK
Length:387
Mass (Da):41,118
Last modified:January 23, 2007 - v2
Checksum:iFAD7D66D100514B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14436 Genomic DNA. Translation: CAA32604.1.
U28377 Genomic DNA. Translation: AAA69093.1.
U00096 Genomic DNA. Translation: AAC75963.1.
AP009048 Genomic DNA. Translation: BAE76990.1.
PIRiS04733. TVECG.
RefSeqiNP_417401.1. NC_000913.3.
YP_491126.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75963; AAC75963; b2926.
BAE76990; BAE76990; BAE76990.
GeneIDi12933840.
947414.
KEGGiecj:Y75_p2857.
eco:b2926.
PATRICi32121266. VBIEscCol129921_3021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14436 Genomic DNA. Translation: CAA32604.1 .
U28377 Genomic DNA. Translation: AAA69093.1 .
U00096 Genomic DNA. Translation: AAC75963.1 .
AP009048 Genomic DNA. Translation: BAE76990.1 .
PIRi S04733. TVECG.
RefSeqi NP_417401.1. NC_000913.3.
YP_491126.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZMR X-ray 2.40 A 1-387 [» ]
ProteinModelPortali P0A799.
SMRi P0A799. Positions 2-387.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36163N.
IntActi P0A799. 15 interactions.
MINTi MINT-1229247.
STRINGi 511145.b2926.

PTM databases

PhosSitei P0810419.

2D gel databases

SWISS-2DPAGE P0A799.

Proteomic databases

PaxDbi P0A799.
PRIDEi P0A799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75963 ; AAC75963 ; b2926 .
BAE76990 ; BAE76990 ; BAE76990 .
GeneIDi 12933840.
947414.
KEGGi ecj:Y75_p2857.
eco:b2926.
PATRICi 32121266. VBIEscCol129921_3021.

Organism-specific databases

EchoBASEi EB0697.
EcoGenei EG10703. pgk.

Phylogenomic databases

eggNOGi COG0126.
HOGENOMi HOG000227107.
InParanoidi P0A799.
KOi K00927.
OMAi AGHPVGK.
OrthoDBi EOG64N9Z0.
PhylomeDBi P0A799.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00185 .
BioCyci EcoCyc:PGK.
ECOL316407:JW2893-MONOMER.
MetaCyc:PGK.
SABIO-RK P0A799.

Miscellaneous databases

EvolutionaryTracei P0A799.
PROi P0A799.

Gene expression databases

Genevestigatori P0A799.

Family and domain databases

Gene3Di 3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPi MF_00145. Phosphoglyc_kinase.
InterProi IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view ]
PANTHERi PTHR11406. PTHR11406. 1 hit.
Pfami PF00162. PGK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000724. Pgk. 1 hit.
PRINTSi PR00477. PHGLYCKINASE.
SUPFAMi SSF53748. SSF53748. 1 hit.
PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli."
    Alefounder P.R., Perham R.N.
    Mol. Microbiol. 3:723-732(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / CS520.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-24.
    Strain: K12 / EMG2.
  5. Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F.
    Submitted (FEB-1996) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-12.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Cited for: PROTEIN SEQUENCE OF 2-5.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
    Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
    Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-84, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPGK_ECOLI
AccessioniPrimary (citable) accession number: P0A799
Secondary accession number(s): P11665, Q2M9R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3