Reviewed, UniProtKB/Swiss-Prot P0A798 (K6PF1_SHIFL)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase isozyme 1
    EC=2.7.1.11
Alternative name(s):
    6-phosphofructokinase isozyme I
    Phosphofructokinase 1
    Phosphohexokinase 1

Gene names

Name:	pfkA
Ordered Locus Names:	SF3994, S3753

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subunit structure	Homotetramer By similarity. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	6-phosphofructokinase isozyme 1 HAMAP MF_00339		PRO_0000111974
Regions
	Nucleotide binding	22 – 26	5	ATP By similarity
	Nucleotide binding	155 – 159	5	ATP By similarity
	Nucleotide binding	172 – 188	17	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Metal binding	186	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	188	1	Magnesium By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A798-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: D03D79F6A5536A41	FASTA	320	34,842
	10 20 30 40 50 60 MIKKIGVLTS GGDAPGMNAA IRGVVRSALT EGLEVMGIYD GYLGLYEDRM VQLDRYSVSD 70 80 90 100 110 120 MINRGGTFLG SARFPEFRDE NIRAVAIENL KKRGIDALVV IGGDGSYMGA MRLTEMGFPC 130 140 150 160 170 180 IGLPGTIDND IKGTDYTIGF FTALSTVVEA IDRLRDTSSS HQRISVVEVM GRYCGDLTLA 190 200 210 220 230 240 AAIAGGCEFV VVPEVEFSRE DLVNEIKAGI AKGKKHAIVA ITEHMCDVDE LAHFIEKETG 250 260 270 280 290 300 RETRATVLGH IQRGGSPVPY DRILASRMGA YAIDLLLAGY GGRCVGIQNE QLVHHDIIDA 310 320 IENMKRPFKG DWLDCAKKLY

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN45428.1. Different initiation. AE014073 Genomic DNA. Translation: AAP18772.1. Different initiation.
RefSeq	NP_709721.2. NP_838961.1.
3D structure databases
SMR	P0A798. Positions 1-320.
ModBase	Search...
Genome annotation databases
GeneID	1026924. 1079965.
GenomeReviews	Gene locus SF3994 in contig AE005674_GR. Gene locus S3753 in contig AE014073_GR.
KEGG	sfl:SF3994. sfx:S3753.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG405044.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN45428.1-MONOMER.
BRENDA	2.7.1.11. 189495.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_SHIFL

Accession

Primary (citable) accession number: P0A798
Secondary accession number(s): P06998

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 7, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)